1. The physical and biochemical effects of pre-rigor high pressure processing of beef.
- Author
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Morton JD, Lee HY, Pearson RG, and Bickerstaffe R
- Subjects
- Animals, Back Muscles metabolism, Back Muscles ultrastructure, Calpain metabolism, Cattle, Cooking, Female, Food Preferences, Glycogen Phosphorylase metabolism, Humans, Hydrogen-Ion Concentration, Male, Mastication, Microscopy, Electron, Transmission, Myofibrils chemistry, Myofibrils metabolism, Myofibrils ultrastructure, New Zealand, Sarcomeres chemistry, Sarcomeres metabolism, Sarcomeres ultrastructure, Tandem Mass Spectrometry, Back Muscles chemistry, Food Quality, Meat analysis, Meat Products analysis, Meat-Packing Industry methods, Stress, Mechanical
- Abstract
High pressure processing (HPP) of pre-rigor longissimus thoracis (strip loin) from prime and bull animals substantially decreased the shear force and improved consumer eating attributes of the final meat product. The improved tenderness in both prime and bull meat was associated with a lower myofibrillar fragmentation index and reduced calpain 1 activity which indicated the mechanism of tenderisation was different from that which occurred in chill aged meat. Light microscopy showed disruption to the fibre packing within the muscle and electron microscopy confirmed significant disruption of the Z discs and M lines and disappearance of the A lines. Thus, HPP is associated with a reduction in the structural integrity and strength of the sarcomeres. These effects were consistent in strip loins sourced from prime and bull stock. HPP also led to the movement of glycogen phosphorylase from the sarcoplasmic fraction to the insoluble myofibrillar fraction in all animals and this was associated with a higher pH at 24 h., (Copyright © 2018 Elsevier Ltd. All rights reserved.)
- Published
- 2018
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