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Objective: This study aims to investigate the potential functions of miR-19a-3p in HCC., Method: We collected serum samples to analyze miR-19a-3p expression. We utilized CCK8 and Transwell assays to access miR-19a-3p's influence on HCC cells malignancy. We used dual-luciferase reporter and western blotting to validate the impact of p53/miR-19 on miR-19/SOX4., Results: The results demonstrated that miR-19a-3p was highly expressed in pre-operative serum samples and HCC cells, which can promote cell proliferation, migration and invasion in HCC under in vitro conditions. Additionally, there was a p53 binding site on the upstream of miR-19a-3p, which was inhibited by p53. SOX4 was the direct gene targeted by miR-19a-3p. The imbalance of p53-miR-19-SOX4 loop was one reason for the progress of HCC., Conclusion: Our findings validate the mechanisms of miR-19a-3p and highlight its potential as a therapeutic target in HCC., Competing Interests: The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (© 2024 The Authors.)

Objectives: This study aimed to compare the analgesic effects of continuous femoral nerve block (FNB), femoral triangle block (FTB), and adductor canal block (ACB) following total knee arthroplasty (TKA). The goal was to identify the most effective nerve block technique among these., Methods: Patients undergoing TKA were randomly assigned to 1 of 3 groups: FNB, FTB, or ACB. Nerve blocks were administered preoperatively, with catheters placed for patient-controlled nerve analgesia (PCNA). The primary end point was the Numeric Rating Scale (NRS) score at movement at 24 hours postsurgery. Secondary end points included NRS scores at rest and movement, quadriceps strength, Timed Up and Go (TUG) test performance, range of motion, effective PCNA utilization, and opioid consumption at various postsurgery time points., Results: Of the 94 valid data sets analyzed (FNB: 31, FTB: 31, ACB: 32), significant differences were observed in the primary end point (H=7.003, P =0.03). Post hoc analysis with Bonferroni correction showed that the FNB group had a significantly lower median pain score (3 [2 to 4]) compared with the ACB group (4 [3 to 5], Bonferroni-adjusted P =0.03). Regarding secondary end points, both the FNB and FTB groups had significantly lower NRS scores than the ACB group at various time points after surgery. Quadriceps strength and TUG completion were better in the FTB and ACB groups. There were no statistically significant differences among the groups for the other end points., Discussion: Continuous FTB provides postoperative analgesia comparable to FNB but with the advantage of significantly less impact on quadriceps muscle strength, a benefit not seen with FNB. Both FTB and ACB are effective in preserving quadriceps strength postoperatively., Competing Interests: The authors declare no conflict of interest., (Copyright © 2024 The Author(s). Published by Wolters Kluwer Health, Inc.)

Kaempferol, also known as kaempferol-3 or kaempferide, is a flavonoid compound that naturally occurs in tea, as well as numerous common vegetables and fruits, including beans, broccoli, cabbage, gooseberries, grapes, kale, strawberries, tomatoes, citrus fruits, brussel sprouts, apples and grapefruit. The present review mainly summarizes the application of kaempferol in treating diseases and the underlying mechanisms that are currently being studied. Due to its anti-inflammatory properties, it may be used to treat numerous acute and chronic inflammation-induced diseases, including intervertebral disc degeneration and colitis, as well as post-menopausal bone loss and acute lung injury. In addition, it has beneficial effects against cancer, liver injury, obesity and diabetes, inhibits vascular endothelial inflammation, protects the cranial nerve and heart function, and may be used for treating fibroproliferative disorders, including hypertrophic scar., (Copyright: © Ren et al.)

A series of Ca-doped lithium vanadates Li 3-x Ca x VO 4 (x=0, 0.01, 0.03, and 0.05) are synthesized successfully through a simple sol-gel method. XRD patterns and energy-dispersive X-ray spectroscopy (EDS) mappings reveal that the doped Ca 2+ ions enter into the lattice successfully and are distributed uniformly throughout the Li 3 VO 4 (LVO) grains. XRD spectra and SEM images show that Ca doping can lead to an enlarged lattice and refined Li 3 VO 4 particles. A small quantity of V ions will transfer from V 5+ to V 4+ in the Ca-doped samples, as demonstrated by the X-ray photoelectron spectroscopy (XPS) analysis, which leads to an increase of an order of magnitude in the electronic conductivity. Improved rate capability and cycling stability are observed for the Ca-doped samples, and Li 2.97 Ca 0.03 VO 4 exhibits the best electrochemical performance among the studied materials. The initial charge/discharge capacities at 0.1 C increase from 480/645 to 527/702 mA h g -1 as x varies from 0 to 0.03. The charge capacity of Li 2.97 Ca 0.03 VO 4 at 1 C retains 95.3 % of its initial value after 180 cycles, whereas the capacity retention is only 40 % for the pristine sample. Moreover, Li 2.97 Ca 0.03 VO 4 maintains a high discharge capacity of 301.7 mA h g -1 at a high discharge rate (4 C), whereas the corresponding value is only 95.2 mA h g -1 for the pristine LVO sample. The enhanced cycling and rate performances are ascribed to the increased lithium ion diffusivity and electrical conductivity induced by Ca doping., (© 2017 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.)