129 results on '"Chen, X.-S."'
Search Results
2. Quantitative image features of gadoxetic acid-enhanced MRI for predicting glypican-3 expression of small hepatocellular carcinoma ≤3 cm.
- Author
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Dong SY, Sun W, Xu B, Wang WT, Yang YT, Chen XS, Zeng MS, and Rao SX
- Subjects
- Humans, alpha-Fetoproteins analysis, Glypicans, Retrospective Studies, Gadolinium DTPA, Magnetic Resonance Imaging methods, Contrast Media, Carcinoma, Hepatocellular diagnostic imaging, Carcinoma, Hepatocellular pathology, Liver Neoplasms pathology
- Abstract
Aim: To explore the value of quantitative image features of gadoxetic acid-enhanced magnetic resonance imaging (MRI) for predicting Gglypican-3 (GPC3) expression of single hepatocellular carcinoma (HCC) ≤3 cm., Materials and Methods: One hundred and forty-nine patients with histopathologically confirmed HCC were included retrospectively. Quantitative image features and clinicopathological parameters were analysed. The significant predictors for GPC3 expression were identified using multivariate logistic regression analyses. Nomograms were constructed from the prediction model and the progression-free survival (PFS) rate was evaluated by the Kaplan-Meier method., Results: The tumour-to-liver signal intensity (SI) ratio on the hepatobiliary phase (HBP; odds ratio [OR] = 0.004; p=0.001), serum alpha-fetoprotein (AFP) > 20 ng/ml (OR=6.175; p<0.001), and non-smooth tumour margin (OR=4.866; p=0.002) were independent significant factors for GPC3 expression. When the three factors were combined, the diagnostic specificity was 97.7% (42/43). The nomogram based on the predictive model performed satisfactorily (C-index: 0.852). Kaplan-Meier curves showed that patients with GPC3-positive HCCs have lower PFS rates than patients with GPC3-negative HCCs (Log-rank test, p=0.006)., Conclusion: The tumour-to-liver SI ratio on the HBP combined with serum AFP >20 ng/ml and non-smooth tumour margin are potential predictive factors for GPC3 expression of small HCC ≤3cm. GPC3 expression is correlated with a poor prognosis in HCC patients., (Copyright © 2023. Published by Elsevier Ltd.)
- Published
- 2023
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3. Genome Sequence Resource of Fusarium proliferatum f. sp. malus domestica MR5, the Causative Agent of Apple Replant Disease.
- Author
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Duan YN, Ma SR, Chen XS, Shen X, Yin CM, and Mao ZQ
- Subjects
- Genomics, Virulence genetics, Malus microbiology, Fusarium
- Abstract
Apple replant disease (ARD) caused by the fungal pathogen Fusarium proliferatum f. sp. malus domestica ( Fpmd ) MR5 brings annual losses to apple production within China. However, the genomic information of the pathogen is not yet available. Here, we obtained the whole-genome sequence of the highly virulent Fpmd MR5 using the Illumina PE150 platform. The genome size was 42.76 Mb and consisted of 9,047 genes. The GC content was 48.80%, and several genes potentially associated with pathogenicity were identified, such as carbohydrate-active enzymes, secreted proteins, and secondary metabolite gene clusters. There were 260 specific virulence factor genes, mainly related to fungal vegetative growth and the production of cell wall-degrading enzymes. These data will aid future studies investigating host-pathogen interactions and help us develop suitable disease management strategies.
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- 2023
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4. [Accuracy of pooling specimens from different individuals in the detection of genital Chlamydia trachomatis infection: a Meta analysis].
- Author
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Jiang TT and Chen XS
- Subjects
- Humans, Sensitivity and Specificity, ROC Curve, Genitalia, Chlamydia trachomatis, Chlamydia Infections diagnosis
- Abstract
Objective: To determine the accuracy of pooled specimens from multiple individuals for detection of infection with Chlamydia trachomatis . Methods: By April 2022, PubMed and Embase searched relevant studies published in peer-reviewed journals. The QUADAS-2 scale of a quality assessment tool was used to assess the quality of the studies. A curve of summary receiver operating characteristic was applied as a comprehensive assessment of diagnosed accuracy. A bivariate mixed-effects model was used for overall value merging in sensitivity and specificity. In addition, the subgroup analyses regarding sample type, testing method, and the number of samples per pool were performed. Results: A total of 14 846 subjects were included in the analysis. Three studies were from the United States, three from Canada, three from Denmark, two from Lithuania, two from India, two from the Netherlands, and one from Australia, Russia, and Singapore. Compared with the individual specimens, the pooled specimens of multiple individuals had an overall sensitivity of 0.98 (95% CI: 0.97-0.99) and specificity of 1.00 (95% CI: 1.00-1.00) for the detection of Chlamydia trachomatis . Results from the subgroup showed that the overall sensitivity of ligase chain reaction was significantly higher than that of PCR in the diagnosis of pooled samples. Conclusion: It is concluded from the published studies that the pooled specimens were substantially consistent with the single specimens in detecting infection with Chlamydia trachomatis .
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- 2022
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5. Discovery of Fusarium proliferatum f. sp. malus domestica Causing Apple Replant Disease in China.
- Author
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Duan YN, Jiang WT, Zhang R, Chen R, Chen XS, Yin CM, and Mao ZQ
- Subjects
- Soil, Malus microbiology, Fusarium genetics, Ascomycota
- Abstract
Apple replant disease (ARD) is the most serious threat facing the apple industry globally. ARD is mainly manifested as decreased plant growth, serious root rot disease, and considerable yield loss. Microbial factors are the dominant factors leading to the occurrence of ARD. Research on soil-borne pathogenic fungi leading to the occurrence of ARD in China is limited. In the present study, we selected 16 replanting orchards from the Northwest Loess region and around the Bohai Gulf. Diseased roots and rhizosphere soil from healthy apple trees and trees showing ARD symptoms were sampled at random. High-throughput sequencing was used to study the fungal communities in the rhizosphere soil, which showed that the composition of the rhizosphere soil fungal community of ARD-symptomatic and healthy apple trees was different. Nectriaceae at the family level and Fusarium at the genus level dominated the rhizosphere soil fungal community in the two regions, while for healthy apple trees, the relative abundance of Mortierella , Minimedusa , Tetracladium , and Chaetomium was higher. Tissue separation and serial dilution were used to separate fungi, and a total of 89 genera and 219 species were obtained, most of which were Fusarium . Fusarium was further confirmed to be the most abundant pathogen species leading to the occurrence of ARD in China through pathogenicity assays. A pathogenicity assay was carried out by the dip-and-cut technique using different host plants. It was found that Fusarium MR5 showed strong aggressiveness to apple rootstocks. Diseased seedlings specifically exhibited chlorosis of the leaves, browning from the edge of the leaf, followed by rolling and yellowing of the leaves, resulting in wilting and eventually death. Strain MR5 was preliminarily identified as F. proliferatum according to the morphological and cultural characteristics. A maximum likelihood analysis of identities based on six gene sequence (ITS, TUB2, IGS, mtSSU, RPB2, and the TEF gene) alignments between the MR5 strain and other strains showed 99 to 100% homology with F. proliferatum . Based on our test results, strain MR5 was identified as F. proliferatum f. sp. malus domestica , which is of great significance for finding new measures to control ARD in China.
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- 2022
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6. [Long-term outcomes and failure patterns of definitive radiotherapy for cervical esophageal carcinoma].
- Author
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Liu X, Luo JW, Zhou ZM, Wu RY, Zhang Y, Wang K, Chen XS, Qu Y, Huang XD, Wang X, Bi N, Feng QF, Lyu JM, Chen DF, Xiao ZF, Xiao JP, Yi JL, and Gao L
- Subjects
- Humans, Retrospective Studies, Prognosis, Treatment Outcome, Chemoradiotherapy methods, Radiotherapy Dosage, Esophageal Neoplasms pathology, Carcinoma drug therapy
- Abstract
Objective: To evaluate the long-term outcomes, failure patterns and prognostic factors of definitive radiotherapy in patients with cervical esophageal carcinoma (CEC). Methods: We retrospectively reviewed the clinical data of 148 CEC patients who treated with definitive radiotherapy in Cancer Hospital of Chinese Academy of Medical Sciences from January 2001 to December 2017. The median radiation dose was 66 Gy (59.4-70 Gy) and 33.1% of patients received concurrent chemotherapy. The Kaplan-Meier method was used to calculate survival rates. The log rank test was used for survival comparison and univariate prognostic analysis. The Cox model was used for multivariate prognostic analysis. Results: The median follow-up time was 102.6 months. The median survival time, 2- and 5-year overall survival (OS) were 22.7 months, 49.9% and 28.3%. The median, 2- and 5-year progression-free survival were 12.6 months, 35.8% and 25.8%. The 2- and 5-year locoregional recurrence-free survival were 59.1% and 50.8%. The 2- and 5-year distant metastases-free survival were 74.6% and 65.9%. Multivariate analysis showed that EQD(2)>66 Gy was the only independent prognostic indicator for OS ( P =0.040). The median survival time and 5-year OS rate significantly improved in patients who received EQD(2)>66 Gy than those who received≤66 Gy (31.2 months vs. 19.2 months, 40.1% vs. 19.1%, P =0.027). A total of 87 patients (58.8%) developed tumor progression. There were 50 (33.8%), 23 (15.5%) and 39 (26.4%) patients developed local, regional recurrence and distant metastases, respectively. Eleven patients (7.4%) underwent salvage surgery, and the laryngeal preservation rate for entire group was 93.9%. Conclusions: Definitive radiotherapy is an effective treatment for cervical esophageal carcinoma with the advantage of larynx preservation. Local recurrence is the major failure pattern. EQD(2)>66 Gy is associated with the improved overall survival.
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- 2022
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7. [Treatment and prognosis analysis of perineural invasion on sinonasal adenoid cystic carcinoma].
- Author
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Wang ZK, Zhang JH, Chen XS, Liu QF, Wang JB, Wu RY, Zhang Y, Wang K, Qu Y, Huang XD, Xiao JP, Gao L, Xu GZ, Yi JL, and Luo JW
- Subjects
- Humans, Prognosis, Proportional Hazards Models, Retrospective Studies, Carcinoma, Adenoid Cystic pathology, Paranasal Sinus Neoplasms pathology, Paranasal Sinus Neoplasms therapy
- Abstract
Objective: To analyze the efficacy of sinonasal adenoid cystic carcinoma (ACC) with perineural invasion (PNI), and explore the prognostic value of PNI on sinonasal adenoid cystic carcinoma. Methods: The clinical data of 105 patients with sinonasal ACC admitted to Cancer Hospital, Chinese Academy of Medical Sciences from January 2000 to December 2016 were retrospectively reviewed. All patients were restaged according to American Joint Committee on Cancer 8th edition. Follow-up visits were conducted to obtain information of treatment failure and survival outcome. The Log rank test was used for univariate analysis of prognostic factors, and Cox regression model was used for multivariate prognostic analysis. Results: The maxillary sinus ( n =59) was the most common primary site, followed by the nasal cavity ( n =38). There were 93 patients with stage Ⅲ-Ⅳ. The treatment modalities included surgery alone ( n =14), radiotherapy alone ( n =13), preoperative radiotherapy plus surgery ( n =10), and surgery plus postoperative radiotherapy ( n =68). The median follow-up time was 91.8 months, the 5-year local control (LC), distant metastasis-free survival (DMFS), progression-free survival (PFS), and overall survival (OS) rates were 72.6%, 73.0%, 52.9% and 78.0%, respectively. There were 33 patients (31.4%) with PNI-positive. The 5-year DMFS, PFS, and OS rates of PNI-positive group were 53.7%, 29.4% and 56.5%, respectively, which were significantly inferior to those of PNI-negative group (80.8%, 63.0% and 86.8%, respectively, P <0.05), while there was no significant difference in the 5-year LC rate between both groups (64.5% vs 76.5%, P =0.273). The multivariate Cox regression analysis showed PNI was one of the poor prognostic factors of DMFS ( HR =3.514, 95% CI: 1.557-7.932), PFS ( HR =2.562, 95% CI: 1.349-4.866) and OS ( HR =2.605, 95% CI: 1.169-5.806). Among patients with PNI-positive, the 5-year LC, PFS and OS rates of patients received surgery combined with radiotherapy were 84.9%, 41.3% and 72.7%, respectively, which were significantly higher than 23.3%, 10.0% and 26.7% of patients receiving surgery or radiotherapy alone ( P <0.05). Conclusion: The presence of PNI increases the risk of distant metastasis in patients with sinonasal ACC. Compared with patients with PNI-negative, the prognosis of patients with PNI-positive is relatively poor, and surgery combined with radiotherapy for PNI-positive sinonasal ACC results in good clinical outcomes.
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- 2022
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8. Protective effects of lycopene on mitochondrial oxidative injury and dysfunction in the liver of aflatoxin B 1 -exposed broilers.
- Author
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Wan XL, Li N, Chen YJ, Chen XS, Yang Z, Xu L, Yang HM, and Wang ZY
- Subjects
- Animal Feed analysis, Animals, Hydrogen Peroxide, Liver metabolism, Lycopene metabolism, Male, Oxidative Stress, Aflatoxin B1 metabolism, Aflatoxin B1 toxicity, Chickens
- Abstract
This study was conducted to investigate the effects of lycopene (LYC) on mitochondrial oxidative injury and dysfunction in the liver of aflatoxin B
1 (AFB1 )-exposed broilers. A total of 192 healthy 1-day-old male broilers were randomly divided into 3 groups with 8 replicates of 8 birds each. Birds in the 3 groups were fed basal diet (control), basal diet with 100 µg/kg AFB1 , and basal diet with 100 µg/kg AFB1 and 200 mg/kg LYC, respectively. The experiment lasted 42 d. The results showed that AFB1 decreased average daily body weight gain (ADG), average daily feed intake, and gain to feed ratio (G :F) compared to the control group, the LYC supplementation increased ADG and G/F compared to AFB1 group (P < 0.05). Broilers in the AFB1 group had lower mitochondrial glutathione (mGSH) concentration and glutathione peroxidase (GSH-Px), manganese superoxide dismutase (MnSOD), and thioredoxin reductase activities, and higher hydrogen peroxide (H2 O2 ) and reactive oxygen species (ROS) concentrations than the control group (P < 0.05). The LYC increased mGSH concentration and GSH-Px and MnSOD activities, and decreased H2 O2 and ROS concentrations compared to AFB1 group (P < 0.05). Broilers fed the AFB1 diet showed increased mitochondrial swelling and decreased adenosine triphosphate concentration than the control group, and LYC had opposite effects (P < 0.05). The AFB1 decreased the activities of mitochondrial electron transfer chain (ETC) complexes I, II, III, and V, downregulated the mRNA expression levels of hepatic MnSOD, thioredoxin 2, thioredoxin reductase, peroxiredoxin-3, peroxisome proliferator-activated receptor γ coactivator 1α, nuclear respiratory factor 1, and mitochondrial transcription factor A compared with the control group (P < 0.05), and LYC increased activities of mitochondrial ETC complexes III and V, and upregulated mRNA expression levels of these genes in comparison to AFB1 group (P < 0.05). In conclusion, the LYC protected broilers from AFB1 -induced liver mitochondrial oxidative injury and dysfunction by stimulating mitochondrial antioxidant capacity and maintaining mitochondrial biogenesis., (Copyright © 2021 The Authors. Published by Elsevier Inc. All rights reserved.)- Published
- 2021
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9. [The characteristics and citation analysis of the publications in National Medical Journal of China during 2016].
- Author
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Zhou Y, Zhang ZW, Guo R, Zhang Y, Huo YF, Zhu Y, Li J, Chen XS, and Lyu XZ
- Subjects
- Beijing, China, Ultrasonography, Bibliometrics, Neurosurgery
- Abstract
Objective: To analyze the characteristics and citations of articles in National Medical Journal of China ( NMJC ) during 2016, and to discuss the academic level and quality of the journal. Methods: All the literature published in NMJC during 2016 were retrieved through the Chinese Medical Citation index, and the citation frequency data in China's core journals of science and technology from January 2017 to December 2018 was obtained through Institute of Scientific and Technical Information of China. The citation status of articles published in NMJC was statistically analyzed by the method of literature metrology. The main indicators included the citation rate of articles published in each year, the citation frequency of all articles, the citation status of individual papers and authors, the regional and high-yield institution distribution of cited authors, and the main citation journals. Results: In 2016, a total of 962 articles were published in 22 columns of NMJC . The total number of published pages was 3 940, and the average number of articles was 4.09 pages. A total of 28 key topics have been published. The total citation was 2 077 times, with 2.16 times per paper. Among them, 322 papers were not cited, accounting for 33.47%. The maximum citation frequency of a single paper was 66 times. There were good citations in the columns of Guidelines, Epidemiology, New technology and methods, Clinical research and Editorial. Oncology, neurology/psychiatry, imaging/ultrasound/radiology, respiratory medicine and orthopedics accounted for a large part [40.43% (389 articles)]. Articles in neurosurgery, respiratory medicine, preventive medicine and gastrointestinal surgery were all cited with high frequency (all ≥2.75 times/article). A total of 483(50.21%) articles had obtained fund support, and the rate (68.12%) of them cited was slightly higher than that of articles without fund support (64.93%). A total of 23 articles were cited ≥10 times, and 16 first authors were cited ≥10 times. In addation, 26 corresponding authors were cited ≥10 times and 10 institutions were cited more than 15 times. The authors of the cited papers were distributed in 29 provinces (autonomous regions, municipalities directly under the central government), and there were more articles and higher total citation frequency in Beijing, Shanghai, Jiangsu and Tianjin. From 2017 to 2018, papers published in NMJC were cited 2 077 times by a total of 490 journals. Conclusions: The guidelines published in NMJC is highly cited. The editorial department should adjust the column setting timely, strengthen the planning of key topic selection and the solicitation and publicity of excellent papers, and further improve the influence of the magazine.
- Published
- 2020
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10. [Analysis on Neisseria gonorrhoeae infection status and related factors in outpatients of sexually transmitted diseases in Shenzhen].
- Author
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Wang HL, Cai YM, Zhang CL, Ye JB, Weng RX, Hong FC, and Chen XS
- Subjects
- Adolescent, Adult, China, Cross-Sectional Studies, Female, Humans, Male, Middle Aged, Outpatients, Sexual Behavior, Young Adult, Gonorrhea, Sexually Transmitted Diseases
- Abstract
Objective: To understand the prevalence of Neisseria gonorrhoeae (NG) infection and related factors in outpatients of sexually transmitted disease (STD) in Shenzhen and provide scientific evidence for targeted intervention. Methods: A cross-sectional study was conducted in STD outpatients who were aged 18-49 years, had had sexual intercourse, had not received antibiotics in the last 2 weeks and sought medical cares in 22 medical institutions in 6 districts of Shenzhen from 15 April, 2018 to 16 May, 2018, their basic information and urine samples were collected, and NG in urine sample was detected by nucleic acid amplification test. Logistic regression analysis was conducted to identify the related factors for NG infection. Results: The information collection and sample collection were completed for 8 324 STD outpatients, among these STD outpatients, 196 were NG positive, with a positive rate of 2.4% (196/8 324). The positive rate of NG in men (5.8%, 148/2 567) was higher than that in women (0.8%, 47/5 742) ( χ (2)=189.43, P <0.05). Aged 24 years or below ( OR =2.11, 95% CI : 1.44-3.09), single/divorced/widowed ( OR =1.98, 95% CI :1.38-2.84), having casual sex in the last 3 months ( OR =1.77, 95% CI :1.29-2.43) were the related factors for NG infection. Conclusions: We found that NG infection rate was high in STD outpatients in Shenzhen. The infection rates in men and in women differed obviously. It is necessary to conduct gonorrhea screening and intervention in STD outpatients with high-risk behaviors, such as extramarital and casual sex behaviors, and standardize the treatment to reduce the incidence and spread of gonorrhea and other STDs.
- Published
- 2020
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11. LINC01535 promotes proliferation and inhibits apoptosis in esophageal squamous cell cancer by activating the JAK/STAT3 pathway.
- Author
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Fang Y, Zhang S, Yin J, Shen YX, Wang H, Chen XS, and Tang H
- Subjects
- Apoptosis, Cell Cycle, Cell Proliferation, Cells, Cultured, Esophageal Neoplasms pathology, Esophageal Squamous Cell Carcinoma pathology, Humans, RNA, Long Noncoding genetics, Signal Transduction, Esophageal Neoplasms metabolism, Esophageal Squamous Cell Carcinoma metabolism, Janus Kinases metabolism, RNA, Long Noncoding metabolism, STAT3 Transcription Factor metabolism
- Abstract
Objective: To detect the expression of long intergenic non-coding ribonucleic acid (LINC) 01535 in esophageal squamous cell cancer (ESCC) tissues and cells, and to investigate the influences of LINC01535 on the proliferation and apoptosis of ESCC cells and the possible mechanism., Patients and Methods: Quantitative real-time polymerase chain reaction (qRT-PCR) was performed to determine the relative expression of LINC01535 in 43 cases of ESCC tissues and human esophageal cancer cells (KYSE30, EC9706, TE-13, and Ecal09) compared with human esophageal mucosal epithelial cells (HET-1A). The esophageal cancer cells with the highest expression were selected and transfected with small interfering RNA (si)-LINC01535 (experimental group) or si-negative control (NC) (control group). The interference efficiency was measured via qRT-PCR assay. Regulatory effects of LINC01535 on cell proliferative capacity was examined through colony formation assay and cell proliferation assay [Cell Counting Kit-8 (CCK-8)]. Cell cycle and apoptosis influenced by LINC01535 were detected via flow cytometry. Western blotting was applied to determine the expression changes in the molecular markers of the Janus kinase/signal transducer and activator of transcription 3 (JAK/STAT3) signaling pathway., Results: LINC01535 expression in ESCC tissues and cells was remarkably higher than that in para-carcinoma tissues and esophageal mucosal epithelial cells. Knockdown of LINC01535 decreased proliferative capacity, arrested cell cycle in G1/G0 phase, and increased apoptotic rate of ESCC cells. The expressions of the molecular markers of the JAK/STAT3 signaling pathway were altered after knockdown of LINC01535. The above results illustrated that LINC01535 accelerated the proliferation but repressed the apoptosis of ESCC cells by regulating the JAK/STAT3 signaling pathway., Conclusions: The expression of LINC01535 is up-regulated in ESCC tissues and cells, and the highly expressed LINC01535 promotes the proliferation and inhibits the apoptosis of ESCC cells by regulating the JAK/STAT3 signaling pathway. Our findings provide new directions for the diagnosis and treatment of esophageal cancer.
- Published
- 2020
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12. Time series analysis and forecasting of chlamydia trachomatis incidence using surveillance data from 2008 to 2019 in Shenzhen, China.
- Author
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Weng RX, Fu HL, Zhang CL, Ye JB, Hong FC, Chen XS, and Cai YM
- Subjects
- China epidemiology, Humans, Incidence, Models, Biological, Retrospective Studies, Seasons, Chlamydia Infections epidemiology, Chlamydia trachomatis isolation & purification
- Abstract
Chlamydia trachomatis (CT) infection has been a major public health threat globally. Monitoring and prediction of CT epidemic status and trends are important for programme planning, allocating resources and assessing impact; however, such activities are limited in China. In this study, we aimed to apply a seasonal autoregressive integrated moving average (SARIMA) model to predict the incidence of CT infection in Shenzhen city, China. The monthly incidence of CT between January 2008 and June 2019 in Shenzhen was used to fit and validate the SARIMA model. A seasonal fluctuation and a slightly increasing pattern of a long-term trend were revealed in the time series of CT incidence. The monthly CT incidence ranged from 4.80/100 000 to 21.56/100 000. The mean absolute percentage error value of the optimal model was 8.08%. The SARIMA model could be applied to effectively predict the short-term CT incidence in Shenzhen and provide support for the development of interventions for disease control and prevention.
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- 2020
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13. A novel 'surgeon-dominated' approach to the design of 3D-printed patient-specific surgical plates in mandibular reconstruction: a proof-of-concept study.
- Author
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Yang WF, Zhang CY, Choi WS, Zhu WY, Li DTS, Chen XS, Du R, and Su YX
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- Bone Plates, Computer-Aided Design, Humans, Mandible, Printing, Three-Dimensional, Mandibular Reconstruction, Plastic Surgery Procedures, Surgeons, Surgery, Computer-Assisted
- Abstract
Three-dimensionally (3D) printed patient-specific surgical plates have been proposed to facilitate mandibular reconstruction and are attracting extensive attention. We have recently reported the high accuracy of 3D-printed patient-specific surgical plates used in head and neck reconstruction. Based on this previous work, the current study proposes a novel 'surgeon-dominated' approach to the design of 3D-printed patient-specific surgical plates. The aim of this proof-of-concept study was to explore the workflow and technical procedures of the surgeon-dominated approach. The workflow includes virtual surgery, the design and printing of patient-specific surgical devices, and real surgery. The prototype of the patient-specific surgical plate was designed by surgeons and further optimized for 3D printing by engineers. Different types of mandibular defect were tested to confirm the wide applicability of this approach. Cases in which this approach was used were reviewed and the duration of time spent on each case studied. Based on a total of 16 patients, the time spent on virtual surgery and plate design was 18.83±13.19hours, and the time taken for 3D printing, post-processing, and product delivery was 162.9±55.15hours. Therefore, this novel surgeon-dominated approach is feasible and time-saving, which would likely promote the wide application of patient-specific surgical plates and lead to a new era of 'digitization and precision' in mandibular reconstruction. ClinicalTrials.gov registration: NCT03057223., (Copyright © 2019 International Association of Oral and Maxillofacial Surgeons. Published by Elsevier Ltd. All rights reserved.)
- Published
- 2020
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14. [Factor analysis of diagnosis and surgical treatment of local regional recurrence in breast cancer patients].
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Jin ZY, Lu YJ, Chen WG, Li YF, Chen XS, and Shen KW
- Subjects
- Adult, Aged, Aged, 80 and over, Breast Neoplasms mortality, China, Disease-Free Survival, Factor Analysis, Statistical, Female, Humans, Lymph Nodes pathology, Lymphatic Metastasis, Middle Aged, Neoplasm Recurrence, Local mortality, Neoplasm Staging, Retrospective Studies, Survival Analysis, Thoracic Wall pathology, Breast Neoplasms pathology, Breast Neoplasms surgery, Mastectomy, Segmental adverse effects, Neoplasm Recurrence, Local diagnosis, Neoplasm Recurrence, Local surgery
- Abstract
Objective: To analyze the association between clinicopathological factors and clinical diagnosis, treatment and surgery of local regional recurrence (LRR) in breast cancer. Methods: A retrospective study was done to evaluate consecutive 7 823 breast cancer LRR cases between January 2009 and August 2018 at Comprehensive Breast Health Center, Ruijin Hospital, Shanghai Jiaotong University School of Medicine. A total of 108 LRR patients were enrolled: 35 cases (32.4%) with ipsilateral breast tumor recurrence (IBTR) after breast conserving surgery, 40 cases (37.0%) of chest wall recurrence (CR), and 33 cases (30.6%) with regional lymph node recurrence (LNR). All patients were female, aged from 26 to 83 years with a mean of 49 years. Clinicopathological factor and its relationship with different sites of LRR and following surgical choice were analyzed by χ(2) test, rank-sum test and Logistic regression. Survival analysis were performed between different LRR patterns and whether undergoing second surgery. Kaplan-Meier survival curves and Log-rank tests demonstrated the distribution of overall survival. Results: Both univariate analysis and multivariate analysis found that axillary lymph nodes (ALN) status ( OR= 7.27, 95% CI: 1.30 to 40.53, P= 0.042) and disease-free interval ( OR= 0.18, 95% CI: 0.06 to 0.60, P= 0.013) were related to different site of LRR. Compared with patients with IBTR, LNR and CR patients had a higher rate of ALN metastasis and a shorter disease-free interval. A total of 36 LRR patients underwent following surgery. In univariate analysis, initial ALN surgery (χ(2)=16.705, P= 0.001), pathological type (χ(2)=7.047, P= 0.03), ALN status (χ(2)=10.812, P= 0.002), disease-free interval (χ(2)=6.118, P= 0.023) and LRR site(χ(2)=19.328, P= 0.000) were associated with surgical treatment for LRR patients. Multivariate analysis demonstrated that only site of LRR was independently associated with surgery ( OR= 0.17, 95% CI: 0.05 to 0.65, P= 0.024). The 5-year overall survival was 100% and 60.1% ( P= 0.018) for LRR patients treated with surgery or not. Furthermore, CR patients had significantly worse overall survival than LNR and IBTR patients, with 5-year overall survival 53.1%, 73.5%, and 100% respectively ( P= 0.021). Conclusions: Initial lymph nodes metastasis and disease-free interval are associated with different site of LRR. LRR site significantly influenced following surgery choice after LRR, which are both related with overall survival after LRR.
- Published
- 2019
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15. [Strategies of surgical and comprehensive management of breast cancer patients with local regional recurrence].
- Author
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Chen XS and Shen KW
- Subjects
- Axilla, Breast Neoplasms pathology, Breast Neoplasms therapy, Female, Humans, Lymph Nodes pathology, Lymphatic Metastasis, Mastectomy, Segmental, Neoplasm Recurrence, Local pathology, Neoplasm Recurrence, Local therapy, Neoplasm Staging, Prospective Studies, Sentinel Lymph Node Biopsy, Breast Neoplasms surgery, Neoplasm Recurrence, Local surgery
- Abstract
Breast conserving surgery and sentinel lymph node biopsy are widely used in early breast cancer treatment. Right now, the mode of local regional recurrence (LRR) has significantly changed and the rate of ipsilateral breast tumor recurrence and axillary lymph node recurrence are steadily increasing. Due to its relatively low incidence of LRR compared with distant metastasis, inconsistent of pre-recurrence treatment, difficulty in surgical treatment, and few prospective clinical studies, there are rising new challenges for clinical management of LRR patients. In this article, based on new theory of LRR, clinical diagnosis and treatment progress, and our own clinical practice experience for LRR breast cancer patients, we propose that we should make pathological diagnosis and do systemic evaluation for LRR disease, then considering it as a curable disease, and integrating local and systemic comprehensive treatment for LRR patients, thus to improve their disease outcome.
- Published
- 2019
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16. Transcriptome analysis of hepatic gene expression and DNA methylation in methionine- and betaine-supplemented geese (Anser cygnoides domesticus).
- Author
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Yang Z, Yang HM, Gong DQ, Rose SP, Pirgozliev V, Chen XS, and Wang ZY
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- Animal Feed analysis, Animals, Avian Proteins metabolism, Betaine administration & dosage, DNA Methylation drug effects, Diet veterinary, Dietary Supplements analysis, Geese blood, Geese growth & development, Geese metabolism, Gene Expression Profiling veterinary, Liver metabolism, Male, Methionine administration & dosage, Random Allocation, Avian Proteins genetics, Betaine metabolism, Epigenesis, Genetic drug effects, Geese genetics, Methionine metabolism, Transcriptome drug effects
- Abstract
Dietary methionine (Met) restriction produces a coordinated series of transcriptional responses in the liver that limits growth performance and amino acid metabolism. Methyl donor supplementation with betaine (Bet) may protect against this disturbance and affect the molecular basis of gene regulation. However, a lack of genetic information remains an obstacle to understand the mechanisms underlying the relationship between Met and Bet supplementation and its effects on genetic mechanisms. The goal of this study was to identify the effects of dietary supplementation of Met and Bet on growth performance, transcriptomic gene expression, and epigenetic mechanisms in geese on a Met-deficient diet. One hundred and fifty 21-day-old healthy male Yangzhou geese of similar body weight were randomly distributed into 3 groups with 5 replicates per treatment and 10 geese per replicate: Met-deficient diet (Control), Control+1.2 g/kg of Met (Met), and Control+0.6 g/kg of Bet (Bet). All geese had free access to the diet and water throughout rearing. Our results indicated that supplementation of 1.2 g/kg of Met in Met-deficient feed increased growth performance and plasma homocysteine (HCY) levels, indicating increased transsulfuration flux in the liver. Supplementation of 0.6 g/kg Bet had no apparent sparing effect on Met needs for growth performance in growing geese. The expression of many genes critical for Met metabolism is increased in Met supplementation group. In the Bet-supplemented group, genes involved in energy production and conversion were up-regulated. Dietary supplementation with Bet and Met also altered DNA methylation. We observed changes in the methylation of the LOC106032502 promoter and corresponding changes in mRNA expression. In conclusion, Met and Bet supplementation in geese affects the transcriptional regulatory network and alters the hepatic DNA methylation of LOC106032502.
- Published
- 2018
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17. Screening of parathyroid gland by high frequency ultrasound and the relationship between recurrent urinary calculi and primary hyperparathyroidism.
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Dai HX, Li JW, Zhang YQ, Chen XS, Huang X, Liu LJ, Yang YP, Liu EQ, Wang JX, Chen XF, Zeng S, Liang T, and Xu XH
- Subjects
- Adolescent, Adult, Aged, Female, Humans, Hyperparathyroidism, Primary surgery, Male, Middle Aged, Parathyroidectomy methods, Radionuclide Imaging methods, Random Allocation, Recurrence, Urinary Calculi surgery, Young Adult, Hyperparathyroidism, Primary diagnostic imaging, Parathyroid Glands diagnostic imaging, Ultrasonography, Doppler, Color methods, Urinary Calculi diagnostic imaging
- Abstract
Objective: To understand the value of high frequency ultrasound in the clinical screening of parathyroid gland, and to summarize the intrinsic relationship between primary hyperparathyroidism and recurrent urinary calculi., Patients and Methods: 98 cases of urinary calculi were randomly selected, and the patients were admitted to our hospital from March 2014 to August 2017. A total of 100 healthy subjects were selected as group B in the same period. High frequency color Doppler ultrasonography scan recorded the results., Results: Among the subjects in group A, 67 (68.37%) showed parathyroid gland, 14 cases (14.29%) had tumor mass in the parathyroid system, 40 cases more than those in group B (40.00%) and 2 cases (2.00%), (p <0.05). There were 10 cases (10.20%) of primary hyperparathyroidism in group A and no cases of primary hyperparathyroidism in group B (p < 0.05). The occurrence of primary hyperparathyroidism was 26.92% (7/26) in the number of cases, with 3 and more cases of urinary calculi, which was higher than that in the first recurrent cases (3/72), (p<0.005)., Conclusions: One of the key causes of recurrent episodes of urinary calculi is primary hyperparathyroidism, which can be applied to high frequency ultrasonography to develop professional screening of parathyroid gland in cases of urinary calculi.
- Published
- 2018
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18. Next-gen sequencing identifies non-coding variation disrupting miRNA-binding sites in neurological disorders.
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Devanna P, Chen XS, Ho J, Gajewski D, Smith SD, Gialluisi A, Francks C, Fisher SE, Newbury DF, and Vernes SC
- Subjects
- Adult, Autistic Disorder genetics, Binding Sites genetics, Bipolar Disorder genetics, Child, Cohort Studies, DNA, Intergenic genetics, Female, Gene Expression Regulation genetics, Genetic Predisposition to Disease, Genetic Variation genetics, Genomics, High-Throughput Nucleotide Sequencing methods, Humans, Language Development Disorders genetics, Male, MicroRNAs genetics, Nervous System Diseases genetics, Schizophrenia genetics, Sequence Analysis methods, 3' Untranslated Regions genetics, Mental Disorders genetics, Neurodevelopmental Disorders genetics
- Abstract
Understanding the genetic factors underlying neurodevelopmental and neuropsychiatric disorders is a major challenge given their prevalence and potential severity for quality of life. While large-scale genomic screens have made major advances in this area, for many disorders the genetic underpinnings are complex and poorly understood. To date the field has focused predominantly on protein coding variation, but given the importance of tightly controlled gene expression for normal brain development and disorder, variation that affects non-coding regulatory regions of the genome is likely to play an important role in these phenotypes. Herein we show the importance of 3 prime untranslated region (3'UTR) non-coding regulatory variants across neurodevelopmental and neuropsychiatric disorders. We devised a pipeline for identifying and functionally validating putatively pathogenic variants from next generation sequencing (NGS) data. We applied this pipeline to a cohort of children with severe specific language impairment (SLI) and identified a functional, SLI-associated variant affecting gene regulation in cells and post-mortem human brain. This variant and the affected gene (ARHGEF39) represent new putative risk factors for SLI. Furthermore, we identified 3'UTR regulatory variants across autism, schizophrenia and bipolar disorder NGS cohorts demonstrating their impact on neurodevelopmental and neuropsychiatric disorders. Our findings show the importance of investigating non-coding regulatory variants when determining risk factors contributing to neurodevelopmental and neuropsychiatric disorders. In the future, integration of such regulatory variation with protein coding changes will be essential for uncovering the genetic causes of complex neurological disorders and the fundamental mechanisms underlying health and disease.
- Published
- 2018
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19. S-1 chemotherapy and intensity-modulated radiotherapy after D1/D2 lymph node dissection in patients with node-positive gastric cancer: a phase I/II study.
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Wang X, Zhao DB, Yang L, Chi Y, Tang Y, Li N, Wang SL, Song YW, Liu YP, Liu WY, Ren H, Zhang T, Wang JY, Chen XS, Fang H, Wang WH, Li YX, and Jin J
- Subjects
- Adult, Aged, Antimetabolites, Antineoplastic administration & dosage, Antimetabolites, Antineoplastic adverse effects, Chemoradiotherapy, Adjuvant adverse effects, Disease-Free Survival, Drug Combinations, Female, Gastrectomy, Humans, Lymph Node Excision, Lymphatic Metastasis, Male, Middle Aged, Oxonic Acid administration & dosage, Oxonic Acid adverse effects, Survival Rate, Tegafur administration & dosage, Tegafur adverse effects, Antimetabolites, Antineoplastic therapeutic use, Neoplasm Recurrence, Local, Oxonic Acid therapeutic use, Radiotherapy, Intensity-Modulated adverse effects, Stomach Neoplasms pathology, Stomach Neoplasms therapy, Tegafur therapeutic use
- Abstract
Background: This phase I/II clinical trial investigated S-1 administered with intensity-modulated radiotherapy (IMRT) as adjuvant therapy for node-positive gastric cancer. Patients had undergone radical resection and D1/D2 lymph node dissection., Methods: In phase I, patients received adjuvant chemoradiotherapy of IMRT (45 Gy in 25 fractions) with concurrent S-1 administered on a dose-escalation schedule to determine the recommended dose (RD). In phase II, the safety and efficacy of the RD of S-1 combined with IMRT were assessed., Results: We consecutively enrolled 73 patients (56 men; median age, 53 years; range, 29-73 years) and the phase I portion of the study included 27 patients. The RD of S-1 administered concomitantly with IMRT was 80 mg m
-2 day-1 orally, twice daily. The phase II analysis included 52 patients (46 new patients plus 6 from phase I). 8 patients (15.4%) developed grade 3 or 4 toxicities. There were 21 recurrence events and 15 deaths (1 bowel obstruction, 14 gastric cancer). Three-year disease-free survival and overall survival were 62.2% (95% confidence interval (CI), 48.5-75.9) and 70.0% (95% CI, 56.3-83.7), respectively. The median time to recurrence was 17.5 months (range, 3.8-42.0). The median time from recurrence to death was 7.0 months (range, 1.5-28.7)., Conclusions: S-1 combined with IMRT adjuvant chemoradiotherapy is safe and efficacious for advanced gastric cancer.- Published
- 2018
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20. Cooperative polariton dynamics in feedback-coupled cavities.
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Yao B, Gui YS, Rao JW, Kaur S, Chen XS, Lu W, Xiao Y, Guo H, Marzlin K-, and Hu C-
- Abstract
The emerging field of cavity spintronics utilizes the cavity magnon polariton (CMP) induced by magnon Rabi oscillations. In contrast to a single-spin quantum system, such a cooperative spin dynamics in the linear regime is governed by the classical physics of harmonic oscillators. It makes the magnon Rabi frequency independent of the photon Fock state occupation, and thereby restricts the quantum application of CMP. Here we show that a feedback cavity architecture breaks the harmonic-oscillator restriction. By increasing the feedback photon number, we observe an increase in the Rabi frequency, accompanied with the evolution of CMP to a cavity magnon triplet and a cavity magnon quintuplet. We present a theory that explains these features. Our results reveal the physics of cooperative polariton dynamics in feedback-coupled cavities, and open up new avenues for exploiting the light-matter interactions.
- Published
- 2017
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21. [Clinical utility study of 21-gene assay in 927 Chinese patients with early breast cancer].
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Wu JY, Fang Y, Lin L, Zong Y, Chen XS, Huang O, He JR, Zhu L, Chen WG, Li YF, and Shen KW
- Subjects
- Aged, Asian People, Breast Neoplasms chemistry, Breast Neoplasms pathology, China, Female, Humans, Immunohistochemistry, Logistic Models, Neoplasm Grading, Neoplasm Staging, Retrospective Studies, Reverse Transcriptase Polymerase Chain Reaction, Breast Neoplasms genetics, Ki-67 Antigen analysis, Receptors, Estrogen analysis, Receptors, Progesterone analysis
- Abstract
Objective: To investigate the distribution patterns of 21-gene assay and its influencing factors in Chinese patients with early breast cancer. Methods: Nine hundred and twenty-seven early breast cancer patients were retrospectively recruited from January 2009 to December 2015 at Ruijin Hospital, Shanghai Jiaotong University School of Medicine. The 21-gene reverse transcriptase-polymerase chain reaction(RT-PCR) assay were conducted in paraffin-embedded tumor tissues to calculate the Recurrence Score(RS). Immunohistochemistry(IHC) assay was used to measure the expression levels of estrogen receptor(ER), progesterone receptor(PR) and Ki-67. Concordances of RT-PCR and IHC results were assessed. Correlations of RS and classical clinicopathological factors were evaluated, and logistic regression were applied to determine independent predictive factors for RS. Results: The median RS of 927 patients was 23(range: 0~90), and the proportions of patients categorized as having a low, intermediate, or high risk were 26.5%, 47.7% and 25.8%, respectively. The distribution of RS varied significantly according to different tumor grade, T stage, PR status, Ki-67 index and molecular subtypes( P <0.05 for all). Grade, PR status and Ki-67 index were independent predictive factors for RS. ER, PR status and Ki-67 index showed significantly correlation between RT-PCR and IHC assays, and the concordance rates for ER and PR status were 98.7% and 87.8%, respectively. Conclusions: RS significantly correlated with tumor grade, T stage, PR status, Ki-67 index and subtypes. Grade, PR status and Ki-67 index can independently predict RS. Remarkable concordances of ER, PR status and Ki-67 index are found between RT-PCR and IHC assays.
- Published
- 2017
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22. [Effect of 21-gene recurrence score on chemotherapy decisions for patients with estrogen receptor-positive, epidermal growth factor receptor 2-negative and lymph node-negative early stage-breast cancer].
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Mao Y, Chen XS, Liang Y, Wu JY, Huang O, Zong Y, Fang Q, He JR, Zhu L, Chen WG, Li YF, Lin L, Fei XC, and Shen KW
- Subjects
- Age Factors, Breast Neoplasms genetics, Breast Neoplasms pathology, Chemotherapy, Adjuvant, China, ErbB Receptors analysis, Female, Humans, Lymph Nodes, Middle Aged, Neoplasm Staging, Breast Neoplasms chemistry, Breast Neoplasms drug therapy, Clinical Decision-Making, Neoplasm Recurrence, Local genetics, Receptor, ErbB-2, Receptors, Estrogen
- Abstract
Objective: To investigate the effect of 21-gene recurrence score on adjuvant chemotherapy decisions for patients with estrogen receptor (ER)-positive, epidermal growth factor receptor 2 (HER-2)-negative and lymph node (LN)-negative early stage-breast cancer. Methods: One hundred and forty-eight patients with ER+ , HER-2- and LN- early stage breast cancer were recruited in the Ruijin hospital, Shanghai Jiao Tong University School of Medicine. The 21-gene recurrence score (RS)assay was performed and systemic therapeutic decisions were made before and after knowing the RS results under multidisciplinary discussion. The effects of RS assay and the other influential factors on adjuvant chemotherapy decision were further analyzed. Results: After knowing the RS results, treatment decisions were changed in 26 out of 148 patients(17.6%). Among them, 9 out of 26 patients were not recommended for chemotherapy; 16 of 26 had treatment recommendation changed to chemotherapy, and chemotherapy regimen was changed in the last one patient. Multivariate analysis showed that RS, age and histological grade were independent factors of decision-making for adjuvant chemotherapy. Conclusion: Our results suggest that 21-gene recurrence score significantly influences decision making for adjuvant chemotherapy in patients with ER+ , HER-2- and LN- early stage breast cancer.
- Published
- 2017
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23. Zigzag C 2 N nanoribbons with edge modifications as multi-functional spin devices.
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Yang XF, Kuang YW, Yu HL, Shao ZG, Zhang J, Feng JF, Chen XS, and Liu YS
- Abstract
Recently, a holey two-dimensional (2D) C
2 N crystal with a wide band gap has been successfully synthesized. However, its non-magnetic property largely limits real applications in spintronics. Here we find that edge magnetism can be introduced by tailoring the holey 2D C2 N crystal into nanoribbons with zigzag edges. When edge N atoms are bare or passivated by H atoms, the device can be used to design high-performance thermospin devices and thermal rectifiers. This is ascribed to the emergence of a spin semiconducting property with a wide band gap. Moreover, if the edge N atoms are passivated by O atoms, the device shows a half-metallic property; meanwhile an obvious spin Seebeck effect can also be observed when a temperature difference is applied across the device.- Published
- 2017
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24. A minicircuitry comprised of microRNA-9 and SIRT1 contributes to leukemogenesis in t(8;21) acute myeloid leukemia.
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Zhou L, Fu L, Lv N, Chen XS, Liu J, Li Y, Xu QY, Huang S, Zhang XD, Dou LP, Wang LL, Li YH, and Yu L
- Subjects
- 3' Untranslated Regions, Cell Line, Tumor, Cell Proliferation, Core Binding Factor Alpha 2 Subunit genetics, Humans, Oncogene Proteins, Fusion genetics, RUNX1 Translocation Partner 1 Protein, Signal Transduction, Translocation, Genetic, Leukemia, Myeloid, Acute genetics, MicroRNAs genetics, Sirtuin 1 genetics
- Abstract
Objective: The AML1-ETO fusion protein (AE) resulting from the t(8;21) translocation is highly related to the pathogenesis and development of leukemia. microRNA-9 (miR-9) acts as a tumor suppressor gene in AE-positive acute myeloid leukemia (AML). Silent mating type information regulation 2 homolog-1 (SIRT1) is overexpressed in most cancer cells by increasing proliferation as a tumorigenic gene. The present study was performed to investigate the underlying interaction between miR-9 and SIRT1 in AE-positive AML., Patients and Methods: Expression of miR-9 and SIRT1 in AE-positive AML patients, healthy donors and AML cell lines were detected by qPCR. Relevance between miR-9 and SIRT1 was assessed by plasmid transfection, Western blot and correlation analysis. Luciferase assay was used to confirm the target gene of miR-9. Knockdown of SIRT1 in different cell lines was achieved by shRNA transfection and CCK-8 assay was used to investigate the effects on cell proliferation., Results: The miR-9 expression was lower in AE-positive cell lines compared to that in other AE-negative AML cell lines, while expression of SIRT1 was higher in AE-positive cell lines. Expression of miR-9 was also downregulated in adult primary t(8;21) AML patients compared to healthy donors. The over-expression of miR-9 decreased luciferase activity of wild-type SIRT1, which was recovered after transfection with mutant SIRT1. The miR-9 directly targets SIRT1 by binding to its 3'-untranslated region and reducing its protein levels. Importantly, miR-9 and SIRT1 mRNA levels were inversely correlated in AE-positive AML cell lines and t(8;21) AML primary leukemia cells. Knockdown of SIRT1 levels using shSIRT1 inhibited cell proliferation in AE-positive AML cell lines., Conclusions: SIRT1 is the target gene of miR-9 and the signaling pathway connecting miR-9 and SIRT1 is a therapeutic target for t(8;21) AML.
- Published
- 2017
25. [Multi-gene assay profiling testing and individualized therapy in breast cancer].
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Chen XS and Shen KW
- Subjects
- Breast Neoplasms genetics, Chemotherapy, Adjuvant, Female, Gene Expression Profiling, Humans, Neoplasm Recurrence, Local, Prognosis, Prospective Studies, Breast Neoplasms therapy, Precision Medicine
- Abstract
Breast cancer is a heterogenetic disease. Multi-gene assay profiling can classify breast cancer into several molecular subtypes, which is better to predict disease prognosis and treatment response. Two prospective clinical trials validated the prognosis predictive value of 21 gene recurrence score and MammaPrint in early breast cancer, than to decrease the prescription of adjuvant chemotherapy and avoid side effect of chemotherapy. New multi-gene assay profiling integrating clinic-pathological factors or reflecting disease development and metastasis can help us predict disease outcome better, thus to achieve individualized therapy in breast cancer.
- Published
- 2017
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26. [Surgical treatment and prognosis of ductal carcinoma in situ: 526 cases analysis].
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Zhu SJ, Chen XS, Wu JY, Huang O, He JR, Zhu L, Chen WG, Li YF, Fei XC, and Shen KW
- Subjects
- Adult, Aged, Axilla, China, Disease-Free Survival, Female, Humans, Kaplan-Meier Estimate, Lymph Node Excision, Lymph Nodes, Mastectomy, Mastectomy, Segmental, Middle Aged, Prognosis, Retrospective Studies, Sentinel Lymph Node Biopsy, Survival Rate, Breast Neoplasms therapy, Carcinoma, Ductal, Breast therapy, Carcinoma, Intraductal, Noninfiltrating therapy
- Abstract
Objective: To evaluate the choice of surgical treatment of ductal carcinoma in situ (DCIS) and its impact on long-term outcomes. Methods: A retrospective analysis of the clinicopathological features and treatment protocol of DCIS patients who underwent surgical treatment in Comprehensive Breast Health Center, Ruijin Hospital, Shanghai Jiaotong University School of Medicine from January 2009 to August 2016 was done. The factors which could affect surgical treatment were analyzed by χ(2) test and Logistic regression. Survival analysis were performed between different surgical approaches. Kaplan-Meier survival curves and Log-rank tests demonstrated the distribution of disease free survival and overall survival. Results: A total of 526 patients were enrolled in this study, 405 cases (77.0%) underwent mastectomy, 121 cases (23.0%) underwent breast-conserving surgery, of which 88 cases received radiotherapy after breast-conserving surgery. It was shown by univariate and multivariate analysis that age>50 years ( OR =0.631, 95% CI: 0.413 to 0.965, P =0.034), first symptom of nipple discharge ( OR =0.316, 95% CI: 0.120 to 0.834, P =0.020), excision biopsy ( OR =1.831, 95% CI: 1.182 to 2.835, P =0.007) and tumor size >3 cm ( OR =0.422, 95% CI: 0.206 to 0.864, P =0.018) were significantly correlated with choice of surgical treatment for breast lesions. Axillary lymph node dissection was performed for 118 cases (22.4%), with sentinel lymph node biopsy for 327 cases (62.2%), and none for 81 cases (15.4%). There was significant statistical difference in the choice of axillary lymph node management in patients of different age (χ(2)=8.124, P =0.017), biopsy type (χ(2)=35.567, P =0.000), breast operation type (χ(2)=149.118, P =0.000) and tumor size (χ(2)=13.394, P =0.010). The 5-year disease free survival rates was 95.7%, 89.6% and 100%, respectively, for mastectomy group, breast-conserving surgery group and breast-conserving surgery plus radiotherapy group. And the 5-year overall survival rates for three groups were 99.0%, 100% and 100%. The differences were not statistically significant ( P =0.427, 0.777). Conclusions: For DCIS patients, age, first symptom and tumor size are independent predictors of breast surgery. The choice of axillary lymph node surgery is influenced by age, biopsy, operation type, and tumor size. Different surgical treatment options has no significant effect on disease-free survival and overall survival in DCIS patients.
- Published
- 2017
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27. [Analysis of the factors influencing adjuvant chemotherapy decisions for triple negative breast cancer].
- Author
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Hong J, Chen XS, Wu JY, Huang O, Zhu L, He JR, Fang Q, Chen WG, Li YF, and Shen KW
- Subjects
- Adult, Aged, Carcinoma, Ductal, Breast pathology, Carcinoma, Ductal, Breast secondary, Chemotherapy, Adjuvant statistics & numerical data, Consensus, Cyclophosphamide administration & dosage, Docetaxel, Epirubicin administration & dosage, Female, Fluorouracil administration & dosage, Humans, Middle Aged, Paclitaxel administration & dosage, Patient Care Team statistics & numerical data, Platinum Compounds administration & dosage, Retrospective Studies, Taxoids administration & dosage, Treatment Outcome, Triple Negative Breast Neoplasms pathology, Antineoplastic Combined Chemotherapy Protocols therapeutic use, Carcinoma, Ductal, Breast drug therapy, Decision Making, Triple Negative Breast Neoplasms drug therapy
- Abstract
Objective: To analyze adjuvant chemotherapy decisions for triple negative breast cancer (TNBC), and explore the influencing factors in the multidisciplinary treatment (MDT) modality. Methods: A retrospective analysis was performed. The cases with invasive TNBC who underwent surgery and MDT discussion for adjuvant treatment in Ruijin Hospital, from April 2013 to June 2015, were recruited. The patients' clinicopathological characteristics were analyzed and adjuvant treatment suggestions from MDT were obtained. Here the chemotherapy decision alteration was defined as a disagreement in chemotherapy or not, or inconsistence in regimens between the attending doctor and the multidisciplinary team. Results: A total of 194 patients aged ≤70 years old were enrolled in the multidisciplinary discussion, and 187 patients (96.4%) were suggested to receive chemotherapy. When compared the opinions of the attending doctor to suggestions of the multidisciplinary team, we found that the percentage of chemotherapy decision alteration reached 22.7% (39/172), of which 94.9% (37/39) were inconsistence in chemotherapy regimens. There were 119 patients who were recommended to receive epirubicin plus cyclophosphamide (EC) followed by docetaxel (T) or weekly paclitaxel (wP) regimens. Before the announcement of results for the E1199 trial, EC-T accounted for 62.5% (55/88), and EC-wP accounted for 37.5% (33/88) for this group of patients. After that, the proportion of EC-T was decreased to 22.6% (7/31) and proportion of EC-wP increased to 77.4%(24/31) ( P <0.001). In addition, a total of 20 patients were suggested to receive platinum based chemotherapy. The proportions were 9.3% in cases with invasive ductal carcinoma, and 33.3% in cases with metaplastic carcinoma, respectively ( P =0.016). Conclusions: The adjuvant chemotherapy decision for TNBC patients is altered in 22.7% of the patients after MDT discussion. After the announcement of SABCS E1199 results, more patients are suggested to receive EC followed by weekly paclitaxel. There is a lack of detailed evidence for platinum based adjuvant chemotherapy for TNBC, and more patients with metaplastic carcinoma receive platinum based adjuvant chemotherapy.
- Published
- 2017
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28. [Strengthening the reform and innovation, promoting the construction of healthy China].
- Author
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Chen XS and Gao RL
- Published
- 2017
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29. Construction of a genetic linkage map and identification of QTL associated with growth traits in Malus sieversii.
- Author
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Liu ZC, Bao DE, Liu DL, and Chen XS
- Subjects
- Chromosomes, Plant, DNA, Plant genetics, Chromosome Mapping, Gene Expression Regulation, Plant, Genetic Linkage, Malus genetics, Malus growth & development, Quantitative Trait Loci
- Abstract
110 F(1) individuals of apple were obtained by crossing between a good quality cultivar'Red Fuji'ofMalus domestica and an accession'Hongrou Apple'ofMalus sieversi. Using Joinmap3.0, a molecular genetic linkage map of Malus sieversii was constructed by 175 SSR and 105 SRAP markers which were integrated into 17 linkage groups and spanned 1299.67cM in genome with an average distance of 4.6cM between the markers. By using interval mapping method,17 QTLs for eight growth traits of Malus sieversii were detected, including 2 QTLs for tree height on cl and c16, 2 QTLs for stem height on c7, 2 QTLs for stem thickness on c3, 2 QTLs for new shoot length on c15 and c16, 2 QTLs for new shoot diameter on c2, 3 QTLs for internode number on c1, c2 and c2, 2 QTLs for internode length on c 1 and c2, 2 QTLs for lenticel density on c17, respectively. The phenotypic variations explained by each QTL ranged from 10.15% to 41.66%, and their LOD values varied from 2.54 to 4.53, of which five QTLs were major effect genes (LOD≥3.5).
- Published
- 2016
30. Carbon-based molecular devices: Fano effects controlled by the molecule length and the gate voltage.
- Author
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Yang XF, Kuang YW, Liu YS, Zhang DB, Shao ZG, Yu HL, Hong XK, Feng JF, Chen XS, and Wang XF
- Abstract
Fano effect is an important quantum phenomenon in mesoscopic systems, which arises from an interference between the localized state and the extended state. Here we observe an obvious Fano effect near the Fermi level in an all-carbon molecular device consisting of an acene molecule sandwiched between two zigzag graphene nanoribbon (ZGNR) electrodes. By increasing the length of the molecule, an extended state gradually evolves into a localized state. With the aid of the nearby extended state, a Fano effect is achieved. Using a gate voltage, we can easily tune the Fano effect induced by the single-transmission channel. When the spin degree of freedom is involved, the all-carbon device can show a half-metallic property with positive or negative 100% spin polarization at the Fermi level under the gate voltage; meanwhile the spin thermoelectric effect can also be enhanced.
- Published
- 2016
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31. Scaled Particle Theory for Multicomponent Hard Sphere Fluids Confined in Random Porous Media.
- Author
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Chen W, Zhao SL, Holovko M, Chen XS, and Dong W
- Abstract
The formulation of scaled particle theory (SPT) is presented for a quite general model of fluids confined in a random porous media, i.e., a multicomponent hard sphere (HS) fluid in a multicomponent hard sphere or a multicomponent overlapping hard sphere (OHS) matrix. The analytical expressions for pressure, Helmholtz free energy, and chemical potential are derived. The thermodynamic consistency of the proposed theory is established. Moreover, we show that there is an isomorphism between the SPT for a multicomponent system and that for a one-component system. Results from grand canonical ensemble Monte Carlo simulations are also presented for a binary HS mixture in a one-component HS or a one-component OHS matrix. The accuracy of various variants derived from the basic SPT formulation is appraised against the simulation results. Scaled particle theory, initially formulated for a bulk HS fluid, has not only provided an analytical tool for calculating thermodynamic properties of HS fluid but also helped to gain very useful insight for elaborating other theoretical approaches such as the fundamental measure theory (FMT). We expect that the general SPT for multicomponent systems developed in this work can contribute to the study of confined fluids in a similar way.
- Published
- 2016
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32. [Factors related to adjuvant ovarian function suppression in premenopausal breast cancer patients].
- Author
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Liang Y, Chen XS, Wu JY, Huang O, Zong Y, Nie LJ, Fang Q, He JR, Zhu L, Chen WG, Li YF, and Shen KW
- Subjects
- Adjuvants, Immunologic, Adult, Age Factors, Aromatase Inhibitors therapeutic use, Breast Neoplasms chemistry, Breast Neoplasms physiopathology, Female, Humans, Middle Aged, Ovary physiopathology, Tamoxifen therapeutic use, Androstadienes therapeutic use, Antineoplastic Agents, Hormonal therapeutic use, Breast Neoplasms drug therapy, Estrogen Antagonists therapeutic use, Ovary drug effects, Premenopause
- Abstract
Objective: To analyze the applied condition of ovary function suppression (OFS) before and after joint analysis of TEXT and SOFT trials and SOFT trial, and to identify the relevant factors of OFS usage., Methods: The analysis was performed in premenopausal women with hormone receptor (HR) positive breast cancer receiving surgical treatment from Apr 2013 to Oct 2015 in Ruijin Hospital, Shanghai Jiaotong University School of Medicine. Adjuvant treatment strategy was made in the multidisciplinary team (MDT) meetings. We analyzed the applied condition of OFS before and after joint analysis, SOFT trial and its relevant factors., Results: Among 454 patients, 114 (25.1%) patients received OFS. Before the results of joint analysis came out, all the patients (38/38) received OFS together with tamoxifen (TAM); after the results came out, clinicians began to put OFS with exemestane into practice, among 76 patients, 41(53.9%) patients received OFS with exemestane while 35 (46.1%) patients received OFS together with TAM. Before the results of SOFT trial came out, 71 out of 310 (22.9%) patients received OFS while 43 out of 144 (29.9%) patients received OFS after that. No significant difference was found between the proportion of patients receiving OFS before and after the results of SOFT trial came out (P=0.112). Age, histological grade, pN status, Ki-67 status, molecular subtype and acceptance of chemotherapy were correlated with OFS treatment (P<0.05). Age, tumor grade and pN were independent significant predictors of OFS usage., Conclusions: After the results of joint analysis came out, clinicians began to apply OFS with exemestane to premenopausal women with HR positive breast cancer. There is no significant difference between the proportion of patients receiving OFS before and after SOFT trial. Age, tumor grade and pN status are independent significant predictors of OFS treatment. Patients younger than 40, with histological grade Ⅱ or Ⅲ tumor and with pN1 or pN2 status are prone to receive OFS.
- Published
- 2016
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33. Evaluation in vivo of autologous cell derived vein grafts based on tissue engineering concept.
- Author
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Wu YF, He FL, Gu YQ, Chen XS, Chen L, Chen L, Zhang J, and Wang ZG
- Subjects
- Animals, Cells, Cultured, Dogs, Female, Male, Organic Chemicals chemistry, Swine, Vena Cava, Inferior, Blood Vessel Prosthesis veterinary, Endothelial Progenitor Cells transplantation, Tissue Engineering methods
- Abstract
Aim: Aim of the study was to construct and evaluate of autologous cell derived vein grafts based on tissue engineering concept., Methods: In this study, we constructed venous grafts (VGs) in 12 days based on tissue engineering concept. We draw out 8-12 mL of bone marrow from the intended recipient canines (N.=8) to culture and expand endothelial progenitor cells (EPCs). After having been labeled with PKH26-GL, the cells were seeded onto the luminal surface of decellularized scaffolds (DSs) with single, rotative method for 4 hours. Following static culture for 24-72 hours, the hybrids were implanted to recipient canine inferior vena cava. Non-seeded DSs (N.=4) were performed as control., Results: Angiography disclosed that patent number of test (control) group were 7/7 (2/4), 6/6 (2/2) and 4/4 (1/2) at postoperative 10 days, 4 weeks and 12 weeks, respectively. At 12 weeks, confluenced endothelial cells which covered the whole inner luminal surface of the explants were detected. Meanwhile, fibroblasts and α-actin positive cells in the matrices were found. PKH26-GL labeled EPCs sustained on the luminal surface accompanied by newly formed endothelial cells. However, the explants in both groups showed partial stenosis., Conclusion: These results indicate that such constructed VGs based on autologous bone marrow-derived EPCs and porcine DSs are promising and deserve to further improvement and testing.
- Published
- 2015
34. Molecular cloning and characterization, and prokaryotic expression of the GnRH1 gene obtained from Jinghai yellow chicken.
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Zhang T, Zhang GX, Han KP, Tang Y, Wang JY, Fan QC, Chen XS, Wei Y, and Wang YJ
- Subjects
- Amino Acid Sequence, Amino Acids chemistry, Amino Acids genetics, Amino Acids metabolism, Animals, Avian Proteins metabolism, Base Sequence, Blotting, Western, Cloning, Molecular, Computational Biology methods, DNA, Complementary chemistry, DNA, Complementary genetics, Gene Expression, Gonadotropin-Releasing Hormone classification, Gonadotropin-Releasing Hormone metabolism, Hydrophobic and Hydrophilic Interactions, Molecular Sequence Data, Phylogeny, Recombinant Proteins metabolism, Sequence Analysis, DNA, Avian Proteins genetics, Chickens genetics, Escherichia coli genetics, Gonadotropin-Releasing Hormone genetics
- Abstract
The gonadotropin-releasing hormone (GnRH) plays an important role in the control of reproductive functions. Recent studies have reported the occurrence of GnRH molecular variants in numerous species. In this study, the GnRH1 gene from Jinghai yellow chicken was cloned by reverse transcriptase-polymerase chain reaction and transformed into BL21 (DE3) competent cells. The GnRH1 gene and amino acid sequences were subjected to bioinformatic analyses. The GnRH1 gene nucleotide sequence was discovered to be 352 bp long, containing a coding, promoter, and section of the 3'-regions. The GnRH1 gene shared 93, 81, 54, 58, 61, 76, 76, 59, 76, and 66% sequence identity with Meleagris gallopavo, Columba livia, Homo sapiens, Bos taurus, swines, Capra hircus, Ovis aries, Pantholops hodgsonii, Equus caballus, and Rattus norvegicus, respectively. The GnRH1 gene showed conserved domains. The GnRH1 protein was a secreted protein comprising 92 amino acids, with a molecular weight of 10205.6 Da and a theoretical pI of 5.67. Most of the amino acid residues were observed to be hydrophilic, indicating water solubility. The predicted secondary structures of proteins included α-helices (h; 23.08%), β-extensions (e; 10.92%), and random coils (c; 66.0%). The successful construction of prokaryotic expression vector pET32a-GnRH1 was confirmed by restriction and sequence analysis. SDS-PAGE analysis showed the successful expression of recombinant plasmid in Escherichia coli BL21 (molecular weight = 25-28 kDa). Larger quantities of protein were expressed in supernatant, indicating greater expression in soluble form. Western blot analysis confirmed the expression of the target protein.
- Published
- 2015
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35. Current noise in three-terminal hybrid quantum point contacts.
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Wu BH, Wang CR, Chen XS, and Xu GJ
- Abstract
We investigate the current noise of three-terminal hybrid structures at arbitrary bias voltages. Our results indicate that the noise can be a useful tool to extract dynamical information in multi-terminal hybrid structures. The zero-frequency noise is sensitive to the coupling with a normal lead. As a result, the characteristic multiple-step structure of the noise Fano factor due to multiple Andreev reflection will be suppressed as we increase this coupling. In addition, the internal dynamics due to processes of Andreev reflection and multiple Andreev reflection raises rich features in the noise spectrum corresponding to the energy differences of various dynamical processes.
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- 2014
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36. Ground penetrating detection using miniaturized radar system based on solid state microwave sensor.
- Author
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Yao BM, Fu L, Chen XS, Lu W, Guo H, Gui YS, and Hu CM
- Subjects
- Copper, Electric Impedance, Time Factors, Microwaves, Miniaturization instrumentation, Radar instrumentation
- Abstract
We propose a solid-state-sensor-based miniaturized microwave radar technique, which allows a rapid microwave phase detection for continuous wave operation using a lock-in amplifier rather than using expensive and complicated instruments such as vector network analyzers. To demonstrate the capability of this sensor-based imaging technique, the miniaturized system has been used to detect embedded targets in sand by measuring the reflection for broadband microwaves. Using the reconstruction algorithm, the imaging of the embedded target with a diameter less than 5 cm buried in the sands with a depth of 5 cm or greater is clearly detected. Therefore, the sensor-based approach emerges as an innovative and cost-effective way for ground penetrating detection.
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- 2013
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37. High prevalence of azithromycin resistance to Treponema pallidum in geographically different areas in China.
- Author
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Chen XS, Yin YP, Wei WH, Wang HC, Peng RR, Zheng HP, Zhang JP, Zhu BY, Liu QZ, and Huang SJ
- Subjects
- Adult, Anti-Bacterial Agents pharmacology, China epidemiology, Cross-Sectional Studies, Drug Resistance, Bacterial, Humans, Male, Prevalence, Treponema pallidum isolation & purification, Azithromycin pharmacology, Syphilis epidemiology, Syphilis microbiology, Treponema pallidum drug effects
- Abstract
Treatment with effective antibiotics is one important strategy for syphilis control in China. This study aimed to evaluate the prevalence of azithromycin resistance to T. pallidum in China. A cross-sectional study was conducted among 391 patients with early syphilis recruited from STD clinics in eight cities during October 2008 and October 2011. The swabs were obtained from the moist lesions of the participating patients. A touchdown/nested PCR of the 23S ribosomal RNA (rRNA) gene was performed on DNA samples extracted from these specimens. The presence or absence of the A2058G point mutation, conferring resistance to azithromycin, was determined by restriction enzyme digestion analysis of the PCR amplicon by MboII. Two hundred and eleven patients with primary or secondary syphilis were found to have T. pallidum DNA in their moist lesions by PCR assays. The A2058G mutation was present in 91.9% (194/211, 95% CI, 87.2-95.1%) of these patients, with no significant differences noted between patients from the eastern part (93.8%), southern part (88.6%) and northern part (95.2%) of China (χ(2) = 2.303, p 0.316). Compared with patients who had not taken macrolides in previous years before study entry, the patients who had taken the antibiotics had a significantly higher prevalence of azithromycin resistance (97.0% vs. 62.5%), with an odds ratio of 19.65 (95% CI, 5.77-66.93). It can be concluded that prevalence of azithromycin resistance is substantial in China and consequently that the macrolides should not be used as a treatment option for early or incubating syphilis in China., (© 2012 The Authors Clinical Microbiology and Infection © 2012 European Society of Clinical Microbiology and Infectious Diseases.)
- Published
- 2013
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38. Single sevoflurane exposure decreases neuronal nitric oxide synthase levels in the hippocampus of developing rats.
- Author
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Feng X, Liu JJ, Zhou X, Song FH, Yang XY, Chen XS, Huang WQ, Zhou LH, and Ye JH
- Subjects
- Animals, Animals, Newborn, Behavior, Animal drug effects, Carbon Dioxide blood, Caspase 3 drug effects, Caspase 3 metabolism, Cell Death drug effects, Hippocampus enzymology, Hippocampus pathology, Male, Maze Learning drug effects, Memory drug effects, Neurons drug effects, Neurons enzymology, Neurons pathology, Nitric Oxide Synthase Type I metabolism, Oxygen blood, Partial Pressure, Rats, Rats, Sprague-Dawley, Sevoflurane, Anesthetics, Inhalation pharmacology, Hippocampus drug effects, Methyl Ethers pharmacology, Nitric Oxide Synthase Type I drug effects
- Abstract
Background: The use of general anaesthetics in young children and infants has raised concerns regarding the adverse effects of these drugs on brain development. Sevoflurane might have harmful effects on the developing brain; however, these effects have not been well investigated., Methods: Postnatal day 7 (P7) Sprague-Dawley rats were continuously exposed to 2.3% sevoflurane for 6 h. We used the Fox battery test and Morris water maze (MWM) to examine subsequent neurobehavioural performance. Cleaved caspase-3 and neuronal nitric oxide synthase (nNOS) were quantified by immunoblotting, and the Nissl staining was used to observe the histopathological changes in the hippocampus., Results: A single 6 h sevoflurane exposure at P7 rats resulted in increased cleaved caspase-3 expression and decreased nNOS levels in the hippocampus, and induced the loss of pyramidal neurones in the CA1 and CA3 subfields of the hippocampus at P7-8. These changes were accompanied by temporal retardation of sensorimotor reflexes. However, neither the Fox battery test at P1-21 nor the MWM test at P28-32 showed differences between the air- and sevoflurane-treated groups., Conclusions: Although early exposure to sevoflurane increases activated caspase-3 expression and neuronal loss and decreases nNOS in the neonatal hippocampus, it does not affect subsequent neurobehavioural performances in juvenile rats.
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- 2012
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39. Pre-combustion CO2 capture by transition metal ions embedded in phthalocyanine sheets.
- Author
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Lü K, Zhou J, Zhou L, Chen XS, Chan SH, and Sun Q
- Abstract
Transition metal (TM) embedded two-dimensional phthalocyanine (Pc) sheets have been recently synthesized in experiments [M. Abel, S. Clair, O. Ourdjini, M. Mossoyan, and L. Porte, J. Am. Chem. Soc. 133, 1203 (2010)], where the transition metal ions are uniformly distributed in porous structures, providing the possibility of capturing gas molecules. Using first principles and grand canonical Monte Carlo simulations, TMPc sheets (TM = Sc, Ti, and Fe) are studied for pre-combustion CO(2) capture by considering the adsorptions of H(2)/CO(2) gas mixtures. It is found that ScPc sheet shows a good selectivity for CO(2), and the excess uptake capacity of single-component CO(2) on ScPc sheet at 298 K and 50 bar is found to be 2949 mg/g, larger than that of any other reported porous materials. Furthermore, electrostatic potential and natural bond orbital analyses are performed to reveal the underlying interaction mechanisms, showing that electrostatic interactions as well as the donation and back donation of electrons between the transition metal ions and the CO(2) molecules play a key role in the capture.
- Published
- 2012
- Full Text
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40. The role of smoking status and collagen IX polymorphisms in the susceptibility to cervical spondylotic myelopathy.
- Author
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Wang ZC, Shi JG, Chen XS, Xu GH, Li LJ, and Jia LS
- Subjects
- Adult, Female, Humans, Male, Middle Aged, Polymerase Chain Reaction, Collagen Type IX genetics, Genetic Predisposition to Disease, Polymorphism, Genetic, Smoking, Spinal Cord Diseases genetics, Spondylosis genetics
- Abstract
We investigated a possible association of collagen IX tryptophan (Trp) alleles (Trp2 and Trp3) and smoking with cervical spondylotic myelopathy (CSM) in 172 Chinese patients and 176 age- and gender-matched controls. The smoking status was evaluated by smoking index (SI). The CSM cases had a significantly higher prevalence of Trp2 alleles (Trp2+) than controls (19.8 vs 6.2%, P = 0.002), but the prevalence of Trp3 alleles (Trp3+) was similar between the two groups (23.3 vs 21.6%, P = 0.713). Logistic regression analyses showed that the subjects with Trp2+ had a higher risk for CSM. We thus analyzed whether smoking status influenced the association between Trp2 alleles and CSM risk. Among Trp2+ subjects with an SI less than 100, the smoking status did not influence the effect of risk for SCM [odds ratio (OR) = 1.34, 95% confidential interval (95%CI) = 0.85-2.18, P > 0.05]. When SI increased from 101 to 300, the OR for CSM reached 3.34 (95%CI = 2.11-5.67, P = 0.011); when SI was more than 300, the OR for CSM reached 5.56 (95%CI = 3.62-7.36, P < 0.001). Among Trp2- subjects with SI more than 300, the OR for CSM increased 2.14 (95%CI = 1.15-4.07, P = 0.024). We found a significant association between the Trp2 alleles and CSM risk and smoking amplifies this risk, suggesting that smoking abstinence is important for reducing CSM occurrence in subjects with high genetic risk.
- Published
- 2012
- Full Text
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41. Observation of electron-antineutrino disappearance at Daya Bay.
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An FP, Bai JZ, Balantekin AB, Band HR, Beavis D, Beriguete W, Bishai M, Blyth S, Boddy K, Brown RL, Cai B, Cao GF, Cao J, Carr R, Chan WT, Chang JF, Chang Y, Chasman C, Chen HS, Chen HY, Chen SJ, Chen SM, Chen XC, Chen XH, Chen XS, Chen Y, Chen YX, Cherwinka JJ, Chu MC, Cummings JP, Deng ZY, Ding YY, Diwan MV, Dong L, Draeger E, Du XF, Dwyer DA, Edwards WR, Ely SR, Fang SD, Fu JY, Fu ZW, Ge LQ, Ghazikhanian V, Gill RL, Goett J, Gonchar M, Gong GH, Gong H, Gornushkin YA, Greenler LS, Gu WQ, Guan MY, Guo XH, Hackenburg RW, Hahn RL, Hans S, He M, He Q, He WS, Heeger KM, Heng YK, Hinrichs P, Ho TH, Hor YK, Hsiung YB, Hu BZ, Hu T, Hu T, Huang HX, Huang HZ, Huang PW, Huang X, Huang XT, Huber P, Isvan Z, Jaffe DE, Jetter S, Ji XL, Ji XP, Jiang HJ, Jiang WQ, Jiao JB, Johnson RA, Kang L, Kettell SH, Kramer M, Kwan KK, Kwok MW, Kwok T, Lai CY, Lai WC, Lai WH, Lau K, Lebanowski L, Lee J, Lee MK, Leitner R, Leung JK, Leung KY, Lewis CA, Li B, Li F, Li GS, Li J, Li QJ, Li SF, Li WD, Li XB, Li XN, Li XQ, Li Y, Li ZB, Liang H, Liang J, Lin CJ, Lin GL, Lin SK, Lin SX, Lin YC, Ling JJ, Link JM, Littenberg L, Littlejohn BR, Liu BJ, Liu C, Liu DW, Liu H, Liu JC, Liu JL, Liu S, Liu X, Liu YB, Lu C, Lu HQ, Luk A, Luk KB, Luo T, Luo XL, Ma LH, Ma QM, Ma XB, Ma XY, Ma YQ, Mayes B, McDonald KT, McFarlane MC, McKeown RD, Meng Y, Mohapatra D, Morgan JE, Nakajima Y, Napolitano J, Naumov D, Nemchenok I, Newsom C, Ngai HY, Ngai WK, Nie YB, Ning Z, Ochoa-Ricoux JP, Oh D, Olshevski A, Pagac A, Patton S, Pearson C, Pec V, Peng JC, Piilonen LE, Pinsky L, Pun CS, Qi FZ, Qi M, Qian X, Raper N, Rosero R, Roskovec B, Ruan XC, Seilhan B, Shao BB, Shih K, Steiner H, Stoler P, Sun GX, Sun JL, Tam YH, Tanaka HK, Tang X, Themann H, Torun Y, Trentalange S, Tsai O, Tsang KV, Tsang RH, Tull C, Viren B, Virostek S, Vorobel V, Wang CH, Wang LS, Wang LY, Wang LZ, Wang M, Wang NY, Wang RG, Wang T, Wang W, Wang X, Wang X, Wang YF, Wang Z, Wang Z, Wang ZM, Webber DM, Wei YD, Wen LJ, Wenman DL, Whisnant K, White CG, Whitehead L, Whitten CA Jr, Wilhelmi J, Wise T, Wong HC, Wong HL, Wong J, Worcester ET, Wu FF, Wu Q, Xia DM, Xiang ST, Xiao Q, Xing ZZ, Xu G, Xu J, Xu J, Xu JL, Xu W, Xu Y, Xue T, Yang CG, Yang L, Ye M, Yeh M, Yeh YS, Yip K, Young BL, Yu ZY, Zhan L, Zhang C, Zhang FH, Zhang JW, Zhang QM, Zhang K, Zhang QX, Zhang SH, Zhang YC, Zhang YH, Zhang YX, Zhang ZJ, Zhang ZP, Zhang ZY, Zhao J, Zhao QW, Zhao YB, Zheng L, Zhong WL, Zhou L, Zhou ZY, Zhuang HL, and Zou JH
- Abstract
The Daya Bay Reactor Neutrino Experiment has measured a nonzero value for the neutrino mixing angle θ(13) with a significance of 5.2 standard deviations. Antineutrinos from six 2.9 GWth reactors were detected in six antineutrino detectors deployed in two near (flux-weighted baseline 470 m and 576 m) and one far (1648 m) underground experimental halls. With a 43,000 ton-GWth-day live-time exposure in 55 days, 10,416 (80,376) electron-antineutrino candidates were detected at the far hall (near halls). The ratio of the observed to expected number of antineutrinos at the far hall is R=0.940±0.011(stat.)±0.004(syst.). A rate-only analysis finds sin(2)2θ(13)=0.092±0.016(stat.)±0.005(syst.) in a three-neutrino framework.
- Published
- 2012
- Full Text
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42. Electronic structures and bonding of graphyne sheet and its BN analog.
- Author
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Zhou J, Lv K, Wang Q, Chen XS, Sun Q, and Jena P
- Abstract
Using density functional theory and generalized gradient approximation for exchange and correlation, we present theoretical analysis of the electronic structure of recently synthesized graphyne and its boron nitride analog (labeled as BN-yne). The former is composed of hexagonal carbon rings joined by C-chains, while the latter is composed of hexagonal BN rings joined by C-chains. We have explored the nature of bonding and energy band structure of these unique systems characterized by sp and sp(2) bonding. Both graphyne and BN-yne are found to be direct bandgap semiconductors. The bandgap can be modulated by changing the size of hexagonal ring and the length of carbon chain, providing more flexibilities of energy band engineering for device applications. The present study sheds theoretical insight on better understanding of the properties of the novel carbon-based 2D structures beyond the graphene sheet.
- Published
- 2011
- Full Text
- View/download PDF
43. Aspergillus vertebral osteomyelitis and ureteral obstruction after liver transplantation.
- Author
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Zhu LP, Chen XS, Wu JQ, Yang FF, and Weng XH
- Subjects
- Administration, Oral, Antifungal Agents therapeutic use, Aspergillosis pathology, Aspergillosis therapy, Humans, Injections, Intravenous, Itraconazole administration & dosage, Itraconazole therapeutic use, Lumbar Vertebrae pathology, Male, Middle Aged, Osteomyelitis etiology, Osteomyelitis pathology, Osteomyelitis therapy, Sputum microbiology, Ureteral Obstruction pathology, Ureteral Obstruction therapy, Aspergillosis etiology, Aspergillus flavus isolation & purification, Liver Transplantation adverse effects, Lumbar Vertebrae microbiology, Osteomyelitis microbiology, Ureteral Obstruction microbiology
- Abstract
Aspergillus osteomyelitis has been reported as a result of dissemination in solid organ transplant recipients. Vertebral osteomyelitis is one of the most common forms of Aspergillus osteomyelitis. An Aspergillus fungal ball is a rare cause of ureteral obstruction. We describe an unusual case of simultaneous vertebral osteomyelitis and ureteral obstruction caused by A. flavus in a hepatic transplant recipient, who was successfully treated with sequential intravenous and oral itraconazole solution., (© 2011 John Wiley & Sons A/S.)
- Published
- 2011
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44. Weekly paclitaxel plus carboplatin is an effective nonanthracycline-containing regimen as neoadjuvant chemotherapy for breast cancer.
- Author
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Chen XS, Nie XQ, Chen CM, Wu JY, Wu J, Lu JS, Shao ZM, Shen ZZ, and Shen KW
- Subjects
- Adult, Aged, Breast Neoplasms metabolism, Breast Neoplasms pathology, Carboplatin administration & dosage, Carcinoma, Ductal, Breast metabolism, Carcinoma, Ductal, Breast secondary, Female, Humans, Immunoenzyme Techniques, In Situ Hybridization, Fluorescence, Lymphatic Metastasis, Middle Aged, Paclitaxel administration & dosage, Receptors, Estrogen metabolism, Receptors, Progesterone metabolism, Survival Rate, Treatment Outcome, Antineoplastic Combined Chemotherapy Protocols therapeutic use, Breast Neoplasms drug therapy, Carcinoma, Ductal, Breast drug therapy, Neoadjuvant Therapy
- Abstract
Background: To evaluate the activity and safety of nonanthracycline-containing weekly PCb [paclitaxel (Taxol) plus carboplatin] regimen in neoadjuvant treatment of breast cancer., Patients and Methods: Eligible patients were assigned to receive four cycles of PCb with dose of paclitaxel 80 mg/m(2) and carboplatin at an area under the curve of 2 mg x min/ml, given day 1, day 8 and day 15 of every 4 weeks. Pathological complete remission (pCR) was defined as no invasive cancer in breast and axillary samples., Results: Overall, 107 consecutive patients received weekly PCb treatment from December 2007 to December 2008, and one was diagnosed with bilateral breast cancer. A total of 85.2% of patients were initially diagnosed with stage III diseases. Clinical response rate was 86.1% with complete remission rate 32.4%. Twenty-one patients achieved pCR after neoadjuvant treatment, with pCR rate 19.4%. The incidence of grade 3-4 neutropenia was 40.2% and only one patient was reported with febrile neutropenia. Severe anemia and thrombocytopenia occurred in 4.7% and 0.9%, respectively, of patients. Peripheral neuropathy was frequent but never severe. Patients with estrogen receptor-negative, progesterone receptor-negative, triple-negative or human epidermal growth factor receptor 2 (Her2)-positive subtype disease had higher pCR., Conclusions: Weekly PCb regimen was very active and tolerable as neoadjuvant treatment of breast cancer. This weekly PCb regimen should consider as a reasonable nonanthracycline-containing option in the neoadjuvant treatment of breast cancer.
- Published
- 2010
- Full Text
- View/download PDF
45. Electric field enhanced hydrogen storage on polarizable materials substrates.
- Author
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Zhou J, Wang Q, Sun Q, Jena P, and Chen XS
- Subjects
- Boron Compounds chemistry, Kinetics, Organosilicon Compounds chemistry, Thermodynamics, Electromagnetic Fields, Energy Transfer, Hydrogen chemistry
- Abstract
Using density functional theory, we show that an applied electric field can substantially improve the hydrogen storage properties of polarizable substrates. This new concept is demonstrated by adsorbing a layer of hydrogen molecules on a number of nanomaterials. When one layer of H(2) molecules is adsorbed on a BN sheet, the binding energy per H(2) molecule increases from 0.03 eV/H(2) in the field-free case to 0.14 eV/H(2) in the presence of an electric field of 0.045 a.u. The corresponding gravimetric density of 7.5 wt% is consistent with the 6 wt% system target set by Department of Energy for 2010. The strength of the electric field can be reduced if the substrate is more polarizable. For example, a hydrogen adsorption energy of 0.14 eV/H(2) can be achieved by applying an electric field of 0.03 a.u. on an AlN substrate, 0.006 a.u. on a silsesquioxane molecule, and 0.007 a.u. on a silsesquioxane sheet. Thus, application of an electric field to a polarizable substrate provides a novel way to store hydrogen; once the applied electric field is removed, the stored H(2) molecules can be easily released, thus making storage reversible with fast kinetics. In addition, we show that materials with rich low-coordinated nonmetal anions are highly polarizable and can serve as a guide in the design of new hydrogen storage materials.
- Published
- 2010
- Full Text
- View/download PDF
46. Comment on "A highly accurate and analytic equation of state for a hard sphere fluid in random porous media".
- Author
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Chen W, Dong W, Holovko M, and Chen XS
- Published
- 2010
- Full Text
- View/download PDF
47. Ferromagnetism in semihydrogenated graphene sheet.
- Author
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Zhou J, Wang Q, Sun Q, Chen XS, Kawazoe Y, and Jena P
- Abstract
Single layer of graphite (graphene) was predicted and later experimentally confirmed to undergo metal-semiconductor transition when fully hydrogenated (graphane). Using density functional theory we show that when half of the hydrogen in this graphane sheet is removed, the resulting semihydrogenated graphene (which we refer to as graphone) becomes a ferromagnetic semiconductor with a small indirect gap. Half-hydrogenation breaks the delocalized pi bonding network of graphene, leaving the electrons in the unhydrogenated carbon atoms localized and unpaired. The magnetic moments at these sites couple ferromagnetically with an estimated Curie temperature between 278 and 417 K, giving rise to an infinite magnetic sheet with structural integrity and magnetic homogeneity. This is very different from the widely studied finite graphene nanostrucures such as one-dimensional nanoribbons and two-dimensional nanoholes, where zigzag edges are necessary for magnetism. From graphene to graphane and to graphone, the system evolves from metallic to semiconducting and from nonmagnetic to magnetic. Hydrogenation provides a novel way to tune the properties with unprecedented potentials for applications.
- Published
- 2009
- Full Text
- View/download PDF
48. Primary molecular features of self-incompatible and self-compatible F(1) seedling from apricot (Prunus armeniaca L.) Katy x Xinshiji.
- Author
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Feng JR, Chen XS, Yuan ZH, Zhang LJ, Ci ZJ, Liu XL, and Zhang CY
- Subjects
- Electrophoresis, Gene Expression Regulation, Plant, Genotype, Proteomics, Prunus, Plant Proteins analysis, Ribonucleases genetics, Seedlings enzymology
- Abstract
Expression of the S-RNase genes in the self-compatible (SC) apricot (Prunus armeniaca L.) cultivar Katy, the self-incompatible (SI) cultivar Xinshiji and their F(1) seedling was examined in this study. Three S-genotypes, S(9)Sc (Sc, self-compatibility S-gene absent from the style), S(8)S(9), and S(8)S(10), were obtained. Seedlings with S-RNase that migrated as a single band in gel electrophoresis were SC, despite high transcript abundance, and those with S-RNase that migrated as two bands were SI with high transcript abundance or SC with low transcript expression. S(8)-RNase was induced in SI cultivars only 24 h after self-pollination, indicating post-transcriptional regulation of S(8)-RNase in SI apricots. A Proteomic study showed that 35 protein spots were synthesized differently between SC and SI pistils. Fifteen of the 35 protein spots were identified; nine proteins, including receptor protein kinase-like protein, reversibly glycosylated polypeptide-2, and isoflavone reductase-like protein, were detected only in the SC pistils; while nine proteins, including actin 7, a putative serine/threonine kinase, and S-RNase, were detected only in the SI pistils. A mitochondrial NAD-dependent malate dehydrogenase and a probable elongation factor G were up-regulated, while heat shock cognate 70 was down-regulated in the SC pistils compared to those in the SI pistils. The results suggest that the proteins responsible for self-compatibility and self-incompatibility may be different.
- Published
- 2009
- Full Text
- View/download PDF
49. Burden of syphilis infections in Shenzhen, China: a preliminary estimation.
- Author
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Hong FC, Feng TJ, Cai YM, Wen LZ, Pan P, Lan LN, Lai YH, Zhou H, Liu XL, Lin SP, Chen G, and Chen XS
- Subjects
- China epidemiology, Female, Homosexuality, Male, Humans, Male, Prevalence, Risk-Taking, Sex Work, Sexual Behavior, Sexually Transmitted Diseases prevention & control, Syphilis prevention & control, Unsafe Sex, Disease Outbreaks, Population Surveillance methods, Sexually Transmitted Diseases epidemiology, Syphilis epidemiology
- Abstract
Information on the prevalence and incidence of sexually transmitted infections is important for developing prevention and control strategies and allocating human and financial resources. However, there are no available estimates of such information for many areas in China. In this study, we used the existing data to make a preliminary estimation of syphilis infections in Shenzhen city, in south-eastern China. Data on prevalence rates of syphilis infections among different populations were obtained from the local HIV/sexually transmitted disease second-generation surveillance programme, and the sizes of different populations were estimated based on the most recently available figures. It was estimated that 83,760 (range 77,490-90,020) people are currently infected with syphilis, giving a prevalence of 0.71-0.82% (0.76% on average) in Shenzhen. Around 18% of these syphilis infections occur among men who have sex with men and another 15.8% and 8.7% occur among female sex workers and their clients, respectively. These estimates suggest that a combination of unprotected paid sex and sex between men may be sustaining the epidemic of syphilis in the study area. The preliminary estimates will assist the government in planning and improving its comprehensive intervention programmes for the future control and prevention of syphilis.
- Published
- 2009
- Full Text
- View/download PDF
50. Prevalence of syphilis and HIV infections among men who have sex with men from different settings in Shenzhen, China: implications for HIV/STD surveillance.
- Author
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Hong FC, Zhou H, Cai YM, Pan P, Feng TJ, Liu XL, and Chen XS
- Subjects
- Adult, China epidemiology, Humans, Male, Prevalence, Young Adult, HIV Infections epidemiology, Homosexuality, Male statistics & numerical data, Syphilis epidemiology
- Abstract
HIV/STD surveillance system data are important for developing prevention and control programmes for men who have sex with men (MSM), now a core group in Chinese HIV/STD surveillance activities. MSM were recruited from different settings in Shenzhen and were tested for HIV and syphilis. A substantial prevalence of HIV and syphilis infections was found in this population. However, risk behaviours and sociodemographic characteristics varied greatly among MSM recruited from the different settings (gay sauna, gay bar and MSM clinic), suggesting that carefully considering and selecting appropriate settings to represent the MSM population is critical for developing HIV and STD surveillance and prevention programmes.
- Published
- 2009
- Full Text
- View/download PDF
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