1. Structure of recombinant Ves v 2 at 2.0 Angstrom resolution: structural analysis of an allergenic hyaluronidase from wasp venom.
- Author
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Skov LK, Seppälä U, Coen JJ, Crickmore N, King TP, Monsalve R, Kastrup JS, Spangfort MD, and Gajhede M
- Subjects
- Amino Acid Sequence, Binding Sites, Crystallography, X-Ray, Glycosylation, Hyaluronoglucosaminidase genetics, Hyaluronoglucosaminidase isolation & purification, Molecular Sequence Data, Protein Folding, Recombinant Proteins chemistry, Recombinant Proteins isolation & purification, Sequence Alignment, Sequence Analysis, Protein, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization, Wasp Venoms genetics, Wasp Venoms isolation & purification, Hyaluronoglucosaminidase chemistry, Models, Molecular, Wasp Venoms chemistry
- Abstract
Wasp venom from Vespula vulgaris contains three major allergens: Ves v 1, Ves v 2 and Ves v 5. Here, the cloning, expression, biochemical characterization and crystal structure determination of the hyaluronidase Ves v 2 from family 56 of the glycoside hydrolases are reported. The allergen was expressed in Escherichia coli as an insoluble protein and refolded and purified to obtain full enzymatic activity. Three N-glycosylation sites at Asn79, Asn99 and Asn127 were identified in Ves v 2 from a natural source by enzymatic digestions combined with MALDI-TOF mass spectrometry. The crystal structure of recombinant Ves v 2 was determined at 2.0 A resolution and reveals a central (beta/alpha)(7) core that is further stabilized by two disulfide bonds (Cys19-Cys308 and Cys185-Cys197). Based on sequence alignments and structural comparison with the honeybee allergen Api m 2, it is proposed that a conserved cavity near the active site is involved in binding of the substrate. Surface epitopes and putative glycosylation sites have been compared with those of two other major group 2 allergens from Apis mellifera (honeybee) and Dolichovespula maculata (white-faced hornet). The analysis suggests that the harboured allergic IgE-mediated cross-reactivity between Ves v 2 and the allergen from D. maculata is much higher than that between Ves v 2 and the allergen from A. mellifera.
- Published
- 2006
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