1. A membrane associated tandem kinase from wild emmer wheat confers broad-spectrum resistance to powdery mildew.
- Author
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Li M, Zhang H, Xiao H, Zhu K, Shi W, Zhang D, Wang Y, Yang L, Wu Q, Xie J, Chen Y, Qiu D, Guo G, Lu P, Li B, Dong L, Li W, Cui X, Li L, Tian X, Yuan C, Li Y, Yu D, Nevo E, Fahima T, Li H, Dong L, Zhao Y, and Liu Z
- Subjects
- Plant Breeding, Genes, Plant, Chromosome Mapping, Disease Resistance genetics, Plant Diseases genetics, Triticum genetics, Ascomycota genetics
- Abstract
Crop wild relatives offer natural variations of disease resistance for crop improvement. Here, we report the isolation of broad-spectrum powdery mildew resistance gene Pm36, originated from wild emmer wheat, that encodes a tandem kinase with a transmembrane domain (WTK7-TM) through the combination of map-based cloning, PacBio SMRT long-read genome sequencing, mutagenesis, and transformation. Mutagenesis assay reveals that the two kinase domains and the transmembrane domain of WTK7-TM are critical for the powdery mildew resistance function. Consistently, in vitro phosphorylation assay shows that two kinase domains are indispensable for the kinase activity of WTK7-TM. Haplotype analysis uncovers that Pm36 is an orphan gene only present in a few wild emmer wheat, indicating its single ancient origin and potential contribution to the current wheat gene pool. Overall, our findings not only provide a powdery mildew resistance gene with great potential in wheat breeding but also sheds light into the mechanism underlying broad-spectrum resistance., (© 2024. The Author(s).)
- Published
- 2024
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