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Investigating urban expansion patterns aids in the management of urbanization and in ameliorating the socioeconomic and environmental issues associated with economic transformation and sustainable development. Applying Harmonized Defense Meteorological Satellite Program-Operational Line-scan System (DMSP-OLS) and the Suomi National Polar-Orbiting Partnership-Visible Infrared Imagery Radiometer Suite (NPP-VIIRS) Nighttime Light (NTL) data, this paper investigated the characteristics of urban landscape in West Africa. Using the harmonized NTL data, spatial comparison and empirical threshold methods were employed to detect urban changes from 1993 to 2018. We examined the rate of urban change and calculated the direction of the urban expansion of West Africa using the center-of-gravity method for urban areas. In addition, we used the landscape expansion index method to assess the processes and stages of urban growth in West Africa. The accuracy of urban area extraction based on NTL data were R 2 = 0.8314 in 2000, R 2 = 0.8809 in 2006, R 2 = 0.9051 in 2012 for the DMSP-OLS and the simulated NPP-VIIRS was R 2 = 0.8426 in 2018, by using Google Earth images as validation. The results indicated that there was a high rate and acceleration of urban landscapes in West Africa, with rates of 0.016 0, 0.017 3, 0.018 9, and 0.068 6, and accelerations of 0.31, 0.42, 0.54, and 0.90 for the periods of 1998-2003, 2003-2008, 2008-2013, and 2013-2018, respectively. The expansion direction of urban agglomeration in West Africa during 1993-2018 was mainly from the coast to inland. However, cities located in the Sahel Region of Africa and in the middle zone expanded from north to south. Finally, the results showed that the urban landscape of West Africa was mainly in a scattered and disordered 'diffusion' process, whereas only a few cities located in coastal areas experiencing the process of 'coalescence' according to urban growth phase theory. This study provides urban planners with relevant insights for the urban expansion characteristics of West Africa., (© Science Press, Northeast Institute of Geography and Agroecology, CAS and Springer-Verlag GmbH Germany, part of Springer Nature 2022.)

2',5'-Oligoadenylate synthetase (2',5'-OAS) is a double-stranded RNA-dependent nucleotidyl-transferase induced by interferon (IFN). Several 2',5'-OAS cDNA have been cloned from human, pig, rat, mouse, and chicken. A P-loop motif followed by an Asp-containing sequence (referred to as D-box) and a region with a high content of Lys and Arg (KR-rich region) are well conserved in 2',5'-OAS. The sequence 196DFLKQR201 of 40-kDa human 2',5'-OAS, to which 8-azido-ATP binds (N. Kon and R.J. Suhadolnik, J. Biol. Chem. 271, 19983-19990, 1996), is included in the KR-rich region. We introduced several site-directed mutations into these active motifs of 42-kDa murine 2',5'-OAS. Each mutant enzyme studied bound to poly(I):poly(C) to the same extent as wild-type enzyme. Both K67R, a P-loop mutant, and K200R, a KR-rich region mutant, exhibited a reduced but considerable rate of enzymatic activities. The activity of the double mutant K67R/K200R was about 10% of the wild type. On the other hand, the activities of both K67M and K200M were not more than 2% of the wild-type enzyme, and no activity was detected in another P-loop mutant, G62A/G63A. The binding of Mg2+ to a D-box mutant D76N/D78N was markedly reduced, and only a very low level of enzymatic activity was detected in this mutant. These results demonstrate that the P-loop, the D-box that binds Mg2+, and the KR-rich region are important for the enzymatic activities of 2',5'-OAS.