1. Insulin resistance, adipokine profile and hepatic expression of SOCS-3 gene in chronic hepatitis C.
- Author
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Wójcik K, Jabłonowska E, Omulecka A, and Piekarska A
- Subjects
- Adult, Biomarkers blood, Biopsy, Case-Control Studies, Enzyme-Linked Immunosorbent Assay, Fatty Liver blood, Fatty Liver genetics, Fatty Liver physiopathology, Fatty Liver virology, Female, Genotype, Hepacivirus genetics, Hepacivirus pathogenicity, Hepatitis C, Chronic complications, Hepatitis C, Chronic diagnosis, Hepatitis C, Chronic genetics, Hepatitis C, Chronic physiopathology, Humans, Inflammation Mediators blood, Interferons, Interleukins genetics, Liver pathology, Liver virology, Liver Cirrhosis blood, Liver Cirrhosis genetics, Liver Cirrhosis physiopathology, Liver Cirrhosis virology, Male, Middle Aged, Polymorphism, Single Nucleotide, RNA, Messenger analysis, Reverse Transcriptase Polymerase Chain Reaction, Risk Factors, Suppressor of Cytokine Signaling 3 Protein, Young Adult, Adipokines blood, Hepatitis C, Chronic blood, Insulin Resistance, Liver chemistry, Suppressor of Cytokine Signaling Proteins genetics
- Abstract
Aim: To analyze adipokine concentrations, insulin resistance and hepatic expression of suppressor of cytokine signaling 3 (SOCS-3) in patients with chronic hepatitis C genotype 1 with normal body weight, glucose and lipid profile., Methods: The study group consisted of 31 patients with chronic hepatitis C and 9 healthy subjects. Total levels of adiponectin, leptin, resistin, visfatin, omentin, osteopontin and insulin were measured using an ELISA kit. The hepatic expression of SOCS-3 was determined by the use of the reverse transcription polymerase chain reaction method., Results: Homeostasis model assessment for insulin resistance (HOMA-IR) values were significantly higher in hepatitis C virus (HCV) infected patients without metabolic disorders compared to healthy controls (2.24 vs 0.59, P = 0.0003). Hepatic steatosis was observed in 32.2% of patients with HCV infection and was found in patients with increased HOMA-IR index (2.81 vs 1.99, P = 0.05) and reduced adiponectin level (5.96 vs 8.37, P = 0.04). Inflammatory activity (G ≥ 2) was related to increased osteopontin concentration (34.04 vs 23.35, P = 0.03). Advanced liver fibrosis (S ≥ 2) was associated with increased levels of omentin and osteopontin (436.94 vs 360.09, P = 0.03 and 32.84 vs 20.29, P = 0.03) and reduced resistin concentration (1.40 vs 1.74, P = 0.047). No correlations were reported between adipokine profile, HOMA-IR values and hepatic expression of the SOCS-3 gene., Conclusion: We speculated that no relationship between adipokines and HOMA-IR values may indicate that HCV can induce insulin resistance itself. Some adipokines appear to be biochemical markers of steatosis, inflammation and fibrosis in patients with chronic HCV infection. © 2014 Baishideng Publishing Group Inc. All rights reserved.
- Published
- 2014
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