1. A three-year evolution and comparison of the bla CTX-M genes in pathogenic and non-pathogenic Escherichia coli isolated from young diarrheic and septicaemic calves in Belgium.
- Author
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Guérin V, Farchi A, Cawez F, Mercuri P, Lucas P, Blanchard Y, Saulmont M, Mainil J, and Thiry D
- Subjects
- Cattle, Animals, Escherichia coli genetics, Belgium epidemiology, beta-Lactamases genetics, Anti-Bacterial Agents, Escherichia coli Infections epidemiology, Escherichia coli Infections veterinary, Escherichia coli Proteins genetics, Cattle Diseases epidemiology
- Abstract
Escherichia coli producing Extended-Spectrum-β-Lactamases (ESBL) are a major public health hazard worldwide. The most frequent ESBL belong to the CTX-M family. This study follows their prevalence in pathogenic and non-pathogenic ESBL-producing E. coli isolated from young diarrheic and septicaemic calves over three calving seasons. The triplex PCR targeted three main groups: CTX-M-1, CTX-M-2 and CTX-M-9. Of the 394 isolates studied, 388 (98.5%) were positive, with a majority of CTX-M-1 (243, 61.7%), following by CTX-M-9 (74, 18.8%) and CTX-M-2 (64, 16.2%). The progressive decrease of ESBL-resistance of pathogenic E. coli is not linked to any shift in genetic background, bla
CTX-M genes still present in 99% of the isolates, or to the proportion of the three CTX-M groups. Moreover, no significant difference was observed in the CTX-M content between pathogenic and non-pathogenic E. coli., Competing Interests: Declaration of Competing Interest None to declare., (Copyright © 2022. Published by Elsevier Ltd.)- Published
- 2022
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