1. A substitution mutation in OsPELOTA confers bacterial blight resistance by activating the salicylic acid pathway.
- Author
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Zhang XB, Feng BH, Wang HM, Xu X, Shi YF, He Y, Chen Z, Sathe AP, Shi L, and Wu JL
- Subjects
- Amino Acid Sequence, Base Sequence, Cell Membrane metabolism, Chromosome Mapping, Cloning, Molecular, Conserved Sequence, Endoplasmic Reticulum metabolism, Gene Expression Regulation, Plant, Genetic Complementation Test, Green Fluorescent Proteins metabolism, Phenotype, Phylogeny, Plant Leaves anatomy & histology, Plant Leaves genetics, Plant Leaves metabolism, Plant Proteins chemistry, Plant Proteins metabolism, Protein Transport, RNA, Messenger genetics, RNA, Messenger metabolism, Disease Resistance genetics, Mutation genetics, Oryza genetics, Oryza microbiology, Plant Diseases genetics, Plant Diseases microbiology, Plant Proteins genetics, Salicylic Acid metabolism
- Abstract
We previously reported a spotted-leaf mutant pelota (originally termed HM47) in rice displaying arrested growth and enhanced resistance to multiple races of Xanthomonas oryzae pv. oryzae. Here, we report the map-based cloning of the causal gene OsPELOTA (originally termed spl
HM47 ). We identified a single base substitution from T to A at position 556 in the coding sequence of OsPELOTA, effectively mutating phenylalanine to isoleucine at position 186 in the translated protein sequence. Both functional complementation and over-expression could rescue the spotted-leaf phenotype. OsPELOTA, a paralogue to eukaryotic release factor 1 (eRF1), shows high sequence similarity to Drosophila Pelota and also localizes to the endoplasmic reticulum and plasma membrane. OsPELOTA is constitutively expressed in roots, leaves, sheaths, stems, and panicles. Elevated levels of salicylic acid and decreased level of jasmonate were detected in the pelota mutant. RNA-seq analysis confirmed that genes responding to salicylic acid were upregulated in the mutant. Our results indicate that the rice PELOTA protein is involved in bacterial leaf blight resistance by activating the salicylic acid metabolic pathway., (© 2017 China National Rice Research Institute. Journal of Integrative Plant Biology published by John Wiley & Sons Australia, Ltd on behalf of Institute of Botany, Chinese Academy of Sciences.)- Published
- 2018
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