1. Identification and functional expression of a new xylose isomerase from the goat rumen microbiome in Saccharomyces cerevisiae.
- Author
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de Souza Colombo G, Viana Mendes I, de Morais Souto B, Chaves Barreto C, Assis Serra L, Ferreira Noronha E, Skorupa Parachin N, Moreira de Almeida JR, and Ferraz Quirino B
- Subjects
- Animals, Fermentation, Phylogeny, Xylose metabolism, Aldose-Ketose Isomerases genetics, Aldose-Ketose Isomerases metabolism, Goats microbiology, Microbiota, Rumen enzymology, Rumen microbiology, Saccharomyces cerevisiae metabolism
- Abstract
The current climate crisis demands replacement of fossil energy sources with sustainable alternatives. In this scenario, second-generation bioethanol, a product of lignocellulosic biomass fermentation, represents a more sustainable alternative. However, Saccharomyces cerevisiae cannot metabolize pentoses, such as xylose, present as a major component of lignocellulosic biomass. Xylose isomerase (XI) is an enzyme that allows xylose consumption by yeasts, because it converts xylose into xylulose, which is further converted to ethanol by the pentose-phosphate pathway. Only a few XI were successfully expressed in S. cerevisiae strains. This work presents a new bacterial XI, named GR-XI 1, obtained from a Brazilian goat rumen metagenomic library. Phylogenetic analysis confirmed the bacterial origin of the gene, which is related to Firmicutes XIs. After codon optimization, this enzyme, renamed XySC1, was functionally expressed in S. cerevisiae, allowing growth in media with xylose as sole carbon source. Overexpression of XySC1 in S. cerevisiae allowed the recombinant strain to efficiently consume and metabolize xylose under aerobic conditions., (© 2022 The Society for Applied Microbiology.)
- Published
- 2022
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