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Objective: This study aims to investigate the bacterial biofilm-inhibitory effect of mushroom extracts., Methods: Mushrooms were collected from Arabuko-Sokoke and Kakamega forests and identified using morphological and molecular approaches. Auricularia auricula-judae , Microporus xanthopus , Termitomyces umkowaani , Trametes elegans , and Trametes versicolor were extracted by chloroform, 70% ethanol, and hot water. Extracts were tested against Escherichia coli , Pseudomonas aeruginosa , and Staphylococcus aureus (ATCC25923). Data were analyzed using SPSS ver. 20.0., Results: Chloroform, 70% ethanol, and hot water extracts of A. auricula-judae (50  μ g/mL) showed statistically significant antibiofilm activities against P. aeruginosa , E. coli , and S. aureus ( p ≤ 0.05). M. xanthopus extracts (250  μ g/mL) revealed significantly significant antibiofilm activities against each test bacterium ( p ≤ 0.05). All extracts of T. umkowaani (250  μ g/mL) exhibited statistically significant antibiofilm activities against S. aureus only ( p ≤ 0.05). Chloroform extract of T. elegans (250  μ g/mL) showed the best antibiofilm activity (69.75 ± 0.01%) against S. aureus. All T. versicolor extracts (250  μ g/mL) indicated the best antibiofilm activities against S. aureus ., Conclusions: Being the first study of its kind to be conducted in Kenya, it added a novel concept to the body of knowledge already known about medical biotechnology research. It offers a fresh understanding of the various varieties of mushrooms found in Kenya, their potential biological function in the production of drugs, particularly those that combat drug resistance, and perhaps even a peek at their bioactive elements. Wild mushrooms, a hidden gem, might help to reopen the pipeline of new antibiotics that have been on the decline. However, further research is required to determine the potential mechanism(s) of action of the extracts that are in charge of the apparent antibiofilm activity., Competing Interests: The authors declare that they have no conflicts of interest., (Copyright © 2024 Gebreselema Gebreyohannes et al.)

Objective: This study was aimed at determining the antioxidant, anti-quorum sensing, and in vitro cytotoxic activities of five wild mushroom extracts., Methods: Wild mushrooms of Auricularia auricula-judae , Termitomyces umkowaani , Trametes elegans , Trametes versicolor, and Microporus xanthopus were collected from Arabuko-Sokoke and Kakamega National Forests, in Kenya. Specimens were identified and extracted using chloroform (CHL), 70% ethanol (Eth), and hot water (HW) solvents. Antioxidant and cytotoxic activities of the extracts were determined using 2,2-diphenyl-1-picrylhydrazyl (DPPH) and Vero cell lines, respectively, while anti-quorum sensing activities were tested against Chromobacterium violaceum . All data were compared using relevant descriptive and inferential statistics at a significance level of p  ≤ 0.05., Results: A total of 35 wild mushrooms were collected, identified, and classified into 14 genera. Among screened mycochemicals, fatty acids, flavonoids, polyphenols, and saponins were detected at higher concentrations. The highest free radical scavenging activities of A. auricula-judae , T. umkowaani , T. elegans , and T. versicolor were observed in 70% Eth extract with the percentage values of 76.40 ± 0.12%, 68.40 ± 0.01%, 62.40 ± 0.07%, and 66.40 ± 0.04%, respectively, whereas the HW extract of Microporus xanthopus showed free radical scavenging activity at 65.90 ± 0.02%. None of the extracts, at the tested concentrations (up to 1000  µ g/mL), had shown cytotoxic activity against the Vero cell line. The HW extract of T. umkowaani and the 70% Eth extract of T. versicolor showed a statistically significant difference in the inhibitory activity of violacein production against C. violaceum at the concentration of 200  µ g/mL., Conclusions: The antioxidant activity of wild mushrooms can help to tackle the diseases caused by free radicals. The anti-quorum sensing potential of wild mushrooms could also provide future alternatives to conventional drug therapies cost-effectively. Further detailed chemistry of the bioactive compounds and their possible mechanisms of action responsible for the observed antioxidant and anti-quorum sensing activities are needed., Competing Interests: The authors declare that they have no conflicts of interest., (Copyright © 2023 Gebreselema Gebreyohannes et al.)