1. Isolation and characterization of the Chrysanthemum nitrate transporter CmNRT1.
- Author
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Gu CS, Zhang XX, Chen SM, Li T, Chen Y, Jiang JF, and Chen FD
- Subjects
- Amino Acid Sequence, Anion Transport Proteins chemistry, Anion Transport Proteins genetics, Anion Transport Proteins isolation & purification, Arabidopsis genetics, Molecular Sequence Data, Nitrate Transporters, Phylogeny, Plant Proteins chemistry, Plants, Genetically Modified, Sequence Alignment, Anion Transport Proteins metabolism, Chrysanthemum metabolism, Plant Proteins metabolism, Plant Roots metabolism
- Abstract
In this study, the nitrate transporter gene CmNRT1 was isolated from the chrysanthemum variety 'Nannongxuefeng'. The full-length cDNA contains an open reading frame of 1761 bp encoding 587 residues. Using qRT-PCR, we found that CmNRT1 was induced by 10 mM NO3(-) in roots and shoots. Two Arabidopsis thaliana transgenic plants expressing CmNRT1 were selected for functional analyses. Root (15)N influx in wild-type and transgenic A. thaliana lines under 10 or 0.2 mM (15)NO3 was tested. Our results indicate that CmNRT1 encodes a constitutive component for a low-affinity transporter.
- Published
- 2016
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