1. Human monoclonal antibodies against Staphylococcus aureus A protein identified by high-throughput single-cell sequencing of phase I clinical volunteers' B cells.
- Author
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Wang W, Gu Y, Ou Y, Zhou J, Liu B, Zuo H, Du Y, Wang Y, Tang T, Zou Q, and Zuo Q
- Subjects
- Humans, Antibodies, Bacterial, Antibodies, Monoclonal, Immunoglobulin G, Methicillin-Resistant Staphylococcus aureus genetics, Staphylococcal Infections, Staphylococcus aureus genetics, Staphylococcus aureus metabolism
- Abstract
Methicillin-resistant Staphylococcus aureus, poses a significant threat through infections in both community and hospital settings. To address this challenge, we conducted a phase I clinical trial study involving a recombinant Staphylococcus aureus vaccine. Utilizing peripheral blood lymphocytes from 64 subjects, we isolated antigen-specific memory B cells for subsequent single-cell sequencing. Among the 676 identified antigen-binding IgG1
+ clones, we selected the top 10 antibody strains for construction within expression vectors. Successful expression and purification of these monoclonal antibodies led to the discovery of a highly expressed human antibody, designated as IgG-6. This antibody specifically targets the pentameric form of the Staphylococcus aureus protein A (SpA5). In vivo assessments revealed that IgG-6 provided prophylactic protection against MRSA252 infection. This study underscores the potential of human antibodies as an innovative strategy against Staphylococcus aureus infections, offering a promising avenue for further research and clinical development., Competing Interests: Declaration of Competing Interest The authors have no financial conflicts of interest., (Copyright © 2023 Elsevier Inc. All rights reserved.)- Published
- 2023
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