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Background: The Gα family plays a crucial role in the complex reproductive regulatory network of teleosts. However, the characterization and function of Gα family members, especially Gαq, remain poorly understood in teleosts. To analyze the characterization, expression, and function of grass carp (Ctenopharyngodon idella) Gαq, we identified the Gα family members in grass carp genome, and analyzed the expression, distribution, and signal transduction of Gαq/gnaq. We also explored the role of Gαq in the reproductive regulation of grass carp., Results: Our results showed that the grass carp genome contains 27 Gα genes with 46 isoforms, which are divided into four subfamilies: Gαs, Gαi/o, Gαq/11, and Gα12/13. The expression level of Cignaq in the testis was the highest and significantly higher than in other tissues, followed by the hypothalamus and brain. The luteinizing hormone receptor (LHR) was mainly localized to the nucleus in grass carp oocytes, with signals also present in follicular cells. In contrast, Gαq signal was mainly found in the cytoplasm of oocytes, with no signal in follicular cells. In the testis, Gαq and LHR were co-localized in the cytoplasm. Furthermore, the grass carp Gαq recombinant protein significantly promoted Cipgr expression., Conclusions: These results provided preliminary evidence for understanding the role of Gαq in the reproductive regulation of teleosts., (© 2024. The Author(s).)

Neonicotinoid insecticides (neonics) are extensively employed in agriculture and pervade various environmental matrices. However, few studies have documented the occurrence and potential chronic ecological risks of these chemicals in the marine environment. We collected 720 seawater samples from Xiangshan Bay during 2015-2019 and the integrated concentrations of seven neonics were determined using the relative potency factor method. Trend analyses using the Mann-Kendall test in time series, along with the estimation of the flux of neonics into the sea, were conducted. At last, the ecological risk of neonics was evaluated by water quality criteria derivation based on species sensitivity distribution. Our findings revealed that 47.6% of samples contained at least one neonic, with the integrated concentration of neonics ranging from 63.30 to 1684.14 ng/L. Imidacloprid and dinotefuran exhibited the highest frequency of detection in the analysis. The significance level of the Mann-Kendall test ranged from 2.16 × 10 -10 to 1.21 × 10 -5 ( S > 0), indicating all neonics behaved with sharply increasing trends. Approximately 8.47 × 10 -2 tons of neonics were discharged into Xiangshan Bay. Notably, the integrated concentrations of neonics represented a potential chronic ecological risk to marine organisms. This study provided novel insights into the spatial distribution, source, and migration of neonic species and their impacts on marine ecosystems.

Cypermethrin (CYP), a prototypical synthetic pyrethroid, reportedly causes metabolic disruption, while its stereoselective impact remains elusive. This study initially revealed that only α-CYP caused significant weight loss at 8.5 mg/(kg•day) in rats. All three CYP isomers caused the accumulation of hepatic glycogen, and hyperlipemia phenotype as the increment of total triglyceride. Rats treated with α-CYP had markedly high blood glucose levels and homeostasis model assessment of insulin resistance index. The systematic inflammation of θ-CYP group rats was evidenced by high lipopolysaccharide-binding protein levels and abnormalities of leukocytes indices. By examining the gut microbiome, we found that α-CYP-treated rats had low contents of Firmicutes and high levels of Verrucomicrobia while Elusimicrobia was enriched in the β-CYP group. The increasing alpha diversity in the θ-CYP group may be due to the dominance of pathogenic bacteria and the increase of probiotics to counteract adverse effects. Exclusively, the α-CYP group enriched total short-chain fatty acids (SCFAs), whereas most SCFAs depleted in the θ-CYP group. The correlation analysis further found Firmicutes, an energy storage modulator, was positive to body weight (BW), while SCFAs exerted the opposite, confirming the low BW in α-CYP. Blood glucose that correlated well with SCFAs and Verrucomicrobia can be accounted for the discrepancy between α-CYP and θ-CYP. Overall, the three isomers exerted stereoselective glycolipid disruption in rats, and gut homeostasis acted as vital indicators., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2022. Published by Elsevier B.V.)

The burden of neonicotinoid insecticides (neonics) in humans has attracted widespread attention in recent years due to the potential adverse effects. Nonetheless, information on the partitioning behavior and distribution in human blood is still limited. Herein, we obtained 115 adult whole blood and plasma specimens for analysis of eight neonics to better understand neonics' partitioning and distribution in human blood. At least one neonic was detected in 49.6% of the red blood cells and 55.7% of the plasma. In red blood cells, the highest detection rate and concentration was thiamethoxam (THI) with 19.1% and 3832 ng/L, respectively. Imidacloprid had the highest detection rate with 26.1% in the plasma. The mass fraction (Fp) of neonics detected indicates that thiacloprid, imidacloprid, and dinotefuran are mostly resided in plasma upon entering into human blood, while thiamethoxam is mostly present in red blood cells. The distribution of clothianidin and acetamiprid between plasma and red blood cells is similar. The mass fraction (Fp) values for THI were significantly different compared to other neonics, and the effect of age and gender on THI partitioning concluded that there may not be significant variability in the distribution of THI in the sampled population. Overall, this study was the first to investigate neonics residuals in red blood cells and provided fundamental information on the partitioning and distribution of neonics in human blood., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 Elsevier Ltd. All rights reserved.)

This research surveyed the concentrations of five organophosphorus pesticides (OPs) in vegetables with the purpose of assessing the potential integrated health risks of residents. From 2018 to 2020, 870 samples of eight kinds of vegetables from Zhejiang Province were collected. Gas chromatography coupled with a flame photometric detector (GC-FPD) analyzed the five OPs. OPs were most frequently detected in celery (18.9% of samples), cowpeas (18.3% of samples), and leeks (16.9% of samples) compared to other vegetables. Among the 11 cities in Zhejiang, the cities with high detection rates of OPs were Ningbo and Hangzhou. The integrated concentrations of OPs in different cities ranged from 71.9 to 376 μg/kg. The cumulative risk assessment revealed that the estimated daily intake (EDI) of leek in Wenzhou was the highest, which was 0.0077 (mg/kg bw) and 0.0059 (mg/kg bw) in adults and children respectively. The health risks of residents who consume these vegetables were within a safe range. The data provided demonstrate the distribution and potential health hazards of OPs in commonly consumed vegetables., (© 2022. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.)

Dietary exposure is the primary route of human exposure to neonicotinoids (neonics), and vegetables are essential foods in people's daily diet. However, the residues and potential health risks of neonics in vegetables from different sources have not been well examined. In this study, we collected 1588 samples of vegetables from organic vegetable bases, farmer's markets, and supermarkets in Zhejiang, China. Three frequently used neonics, acetamiprid, imidacloprid, and thiamethoxam, were selected and analyzed. We investigated the residue, temporal and spatial distribution, and potential health risks. Cowpea was detected with the highest mean imidacloprid-equivalent total neonics (IMI RPF ) by value of 655 μg/kg. Vegetable samples from farmer's markets were detected with the highest mean IMI RPF by value of 168 μg/kg, followed by supermarkets (134 μg/kg) and the lowest in organic vegetable bases (76.9 μg/kg). The outcomes of integrated risk assessment for dietary intake of those three neonics in vegetables were all within the safety., (© 2022. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.)

The usage of Organophosphorus flame retardants (OPFRs) is gradually increased as the ban on brominated flame retardants (BFRs) worldwide. The frequent accessibility of OPFRs in aquatic environment poses potential risk to human. Previous studies have concerned on surface water, while studies on tap water are limited. In this research, we aim to evaluate the removal efficiency of the tap water treatment process and investigate the exposure risk of OPFRs in tap water. Herein, we collected 14 samples from water source, 10 samples from water treatment plants and 47 from tap to analyze the concentrations and removal efficiency of OPFRs in Hangzhou tap water supply system. The results showed the concentrations of ∑OPFRs ranged from 9.25 to 224.74 ng/L in all samples, with Tris(1-chloro-2-propyl) Phosphate (TCPP), Tris(2-chloroethyl) phosphate (TCEP), Triphenyl phosphate (TPHP), and Tributyl phosphate (TBP) being the predominant compounds. Levels of the OPFRs had a 10.0 % - 50.4 % declination when compared samples after treatment with that before. The maximum exposure doses of ∑OPFRs via tap water for both adults and children were much lower than the reference dose (RfD). As a result, the hazard index (HI) and the carcinogenic risk (CR) pinpointed a negligible non-carcinogenic and carcinogenic risk for the residents. Even so, given the pervasive usage of OPFRs, the residual levels and the potential risk of OPFRs in watershed should be continuously concerned., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2022 Elsevier B.V. All rights reserved.)

The different physicochemical properties of various agro-waste biomasses require a diversity of bioenergy utilization patterns. This study investigated the characteristics of a total of 74 manures and 78 crop straw samples from East China to identify the primary characteristic indicators that are essential to distinguish specific agro-wastes from others. Principal component analysis was applied, to discover critical features of biomass for the decision-making regarding the bioenergy production mode. The results identified the following four principal components of manures: "organic nutrients", "metals", "bioavailability of nutrients", and "toxic potential". For crop straws these were "lignocellulose/plant strength elements", "organics/inorganic metals for chlorophyll", "C/N", and "Na/Zn/fixed C". Considering the practical application significance of anaerobic digestion (AD) in the ecological civilization construction in rural China, the theoretical bio-methane potential was calculated based on the average values of different agro-biomasses from different areas. The results were 335.5-620.4 STP mL/g VS for manures and 434.0-540.3 STP mL/g VS for crop straws., (Copyright © 2019 Elsevier Ltd. All rights reserved.)