Search

The microalgal-bacterial granular sludge (MBGS) process, enhanced with sodium bicarbonate (NaHCO 3 ), offers a sustainable alternative for wastewater treatment aiming for carbon neutrality. This study demonstrates that NaHCO 3 , which can be derived from the flue gases and alkaline textile wastewater, significantly enhances pollutant removal and biomass production. Optimal addition of NaHCO 3 was found to achieve an inorganic-to-organic carbon ratio of 1.0 and a total carbon-to-nitrogen ratio of 5.0. Metagenomic analysis and structural equation modeling showed that NaHCO 3 addition increased dissolved oxygen concentrations and pH levels, creating a more favorable environment for key microbial communities, including Proteobacteria, Chloroflexi, and Cyanobacteria. Confocal laser scanning microscopy further confirmed enhanced interactions between Cyanobacteria and Proteobacteria/Chloroflexi, facilitating the MBGS process. These microbes harbored functional genes (gap2, GLU, and ppk) critical for removing organics, nitrogen, and phosphorus. Carbon footprint analysis revealed significant reductions in CO 2 emissions by the NaHCO 3 -added MBGS process in representative countries (China, Australia, Canada, Germany, and Morocco), compared to the conventional activated sludge process. These findings highlight the effectiveness of NaHCO 3 in optimizing MBGS process, establishing it as a key strategy in achieving carbon-neutral wastewater treatment globally., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier Ltd. All rights reserved.)

Autotrophic microorganisms, the pivotal carbon fixers, exhibit a broad distribution across diverse environments, playing critical roles in the process of carbon sequestration. However, insights into their distribution characteristics in aquifers, particularly in those petroleum-hydrocarbon-contaminated (PHC) aquifers that were known for rich in heterotrophs, have been limited. In the study, groundwater samples were collected from red clay aquifers in the storage tank leakage area of a PHC site, a prevalent aquifer type in southern China and other regions. Metagenomics combined with hydrochemical and inorganic carbon isotope analyses were employed to elucidate the presence of microbial carbon fixation and its driving forces. Results showed that there were hundreds of autotrophic microorganisms participating in distinct carbon fixation processes in the red clay PHC aquifers. Reductive tricarboxylic acid (rTCA) and dicarboxylate/4-hydroxybutyrate (DC/4HB), as well as 3-hydroxypropionate (3HP or/and 3-hydroxypropionate/4-hydroxybutyrate (3HP/4HB)) were the predominant carbon fixation pathways. The abundances of carbon fixation genes and autotrophic microorganisms were significantly and positively correlated with hydrocarbon concentrations and δ 13 C of dissolved inorganic carbon (δ 13 C-DIC) values. This finding indicated that the petroleum hydrocarbon significantly promoted the proliferation of carbon fixation microorganisms, leading to a substantial uptake of inorganic carbon. Therefore, we deduce that this process holds considerable potential for carbon sequestration in PHC-contaminated aquifers., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier Ltd. All rights reserved.)

The sequestration of soil inorganic carbon (SIC) especially pedogenic carbonate (PC) is one of the important pathways reducing the concentration of atmospheric carbon dioxide and thus mitigating climate change in coastal areas. Using the technology of 13 C stable isotope, we analyzed the differences in the composition and storage of SIC, and explored the key physicochemical properties influencing soil PC storage in different horizons (0-10, 11-20, 21-40, 41-60, 61-80 and 81-100 cm) from Suaeda salsa wetland (SS), Spartina alterniflora wetland (SA), young poplar plantation (YP), and mature poplar plantation (MP) in coastal area of the northern Jiangsu Province. The results showed that except for the surface (0-10 cm) soil in MP, the SIC content was higher than SOC in all soil horizons. Overall, neither the soil PC to SIC ratio nor the SIC storage were significantly different in SA and SS soils. Compared to wetland soils (0-40 cm), the soil PC to SIC ratio was reduced by 32.7% and 54.1% and the PC storage was reduced by 40.5% and 59.2%, the lithogenic carbonate (LC) storage changed little, while the SIC storage was reduced by 21.0% and 17.9%, respectively in the YP and MP soils. Compared to the YP soils (0-100 cm), both the soil PC to SIC ratio and the PC storage were significantly reduced while the LC storage was significantly increased, especially at the 41-100 cm soil horizons, meanwhile, the SIC storage was not significantly changed in the MP soils. Results of the structural equation modeling (SEM) indicated that key factors influencing soil PC storage were the ratio of PC to SIC, followed by the SOC content and bulk density. SOC could inhibit the formation of soil PC. Generally, the coastal wetlands have greater SIC storage and sequestration potential than poplar plantations, and the PC sequestration can be regulated by modulating the ratio of PC to SIC and SOC content.

Ammonium removal by a symbiosis system of algae (Chlorella vulgaris) and nitrifying bacteria was evaluated in a long-term photo-sequencing batch reactor under varying influent inorganic carbon (IC) concentrations (15, 10, 5 and 2.5 mmol L -1 ) and different nitrogen loading rate (NLR) conditions (270 and 540 mg-N L -1 d -1 ). The IC/N ratios provided were 2.33, 1.56, 0.78 and 0.39, respectively, for an influent NH 4 + -N concentration of 90 mg-N L -1 (6.43 mmol L -1 ). The results confirmed that both ammonium removal and N 2 O production were positively related with IC concentration. Satisfactory ammonium removal efficiencies (>98 %) and rates (29-34 mg-N gVSS -1  h -1 ) were achieved regardless of NLR levels under sufficient IC of 10 and 15 mmol L -1 , while insufficient IC at 2.5 mmol L -1 led to the lowest ammonium removal rates of 0 mg-N gVSS -1  h -1 . The ammonia oxidation process by ammonia oxidizing bacteria (AOB) played a predominant role over the algae assimilation process in ammonium removal. Long-time IC deficiency also resulted in the decrease in biomass and pigments of algae and nitrifying bacteria. IC limitation led to the decreasing N 2 O production, probably due to its negative effect on ammonia oxidation by AOB. The optimal IC concentration was determined to be 10 mmol L -1 (i.e., IC/N of 1.56, alkalinity of 500 mg CaCO 3 L -1 ) in the algae-bacteria symbiosis reactor, corresponding to higher ammonia oxidation rate of ∼41 mg-N gVSS -1  h -1 and lower N 2 O emission factor of 0.13 %. This suggests regulating IC concentrations to achieve high ammonium removal and low carbon emission simultaneously in the algae-bacteria symbiosis wastewater treatment process., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier B.V. All rights reserved.)

The difficulty of the microbial conversion process for the degradation of sotol vinasse due to its high acidity and organic load makes it an effluent with high potential for environmental contamination, therefore its treatment is of special interest. Calcium carbonate is found in great abundance and has the ability to act as a neutralizing agent, maintaining the alkalinity of the fermentation medium as well as, through its dissociation, releasing CO 2 molecules that can be used by phototrophic CO 2 -fixing bacteria. This study evaluated the use of Rhodopseudomonas telluris (OR069658) for the degradation of vinasse in different concentrations of calcium carbonate (0, 2, 4, 6, 8 and 10% m/v). The results showed that calcium carbonate concentration influenced volatile fatty acids (VFA), alkalinity and pH, which in turn influenced changes in the degradation of chemical oxygen demand (COD), phenol and sulfate. Maximum COD and phenol degradation values of 83.16 ± 0.15% and 90.16 ± 0.30%, respectively, were obtained at a calcium carbonate concentration of 4%. At the same time, the lowest COD and phenol degradation values of 52.01 ± 0.38% and 68.21 ± 0.81%, respectively, were obtained at a calcium carbonate concentration of 0%. The data obtained also revealed to us that at high calcium carbonate concentrations of 6-10%, sotol vinasse can be biosynthesized by Rhodopseudomonas telluris (OR069658) to VFA, facilitating the degradation of sulfates. The findings of this study confirmed the potential for using Rhodopseudomonas telluris (OR069658) at a calcium carbonate concentration of 4% as an appropriate alternative treatment for sotol vinasse degradation., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Published by Elsevier Ltd.)

The anaerobic ammonium oxidation (anammox) process is adversely affected by the limitation of inorganic carbon (IC). In this research, a new technique was introduced to assist anammox biomass in counteracting the adverse effects of IC limitation by incorporating waste iron scraps (WIS), a cheap and easily accessible byproduct of lathe cutting. Results demonstrated that reducing the influent IC/TN ratio from 0.08-0.09 to 0.04 resulted in a 20 % decrease in the nitrogen removal rate (NRR) for the control reactor, with an average specific anammox activity (SAA) of 0.65 g N/g VSS/day. Nevertheless, the performance of the WIS-assisted anammox reactor remained robust despite the reduction in IC supply. In fact, the NRR and SAA of the WIS-assisted reactor exhibited substantial improvements, reaching approximately 1.86 kg/(m 3 ·day) and 0.98 g N/g VSS/day, respectively. These values surpassed those achieved by the control reactor by approximately 39 % and 51 %, respectively. The microbial analysis confirmed that the WIS addition significantly stimulated the proliferation of anammox bacteria (dominated by Candidatus Kuenenia) under IC limitation. The anammox gene abundances in the WIS-assisted anammox reactor were 3-4 times higher than those in the control reactor. Functional genes prediction based on the KEGG database revealed that the addition of WIS significantly enhanced the relative abundances of genes associated with nitrogen metabolism, IC fixation, and central carbon metabolism. Together, the results suggested that WIS promoted carbon dioxide fixation of anammox species to resist IC limitation. This study provided a promising approach for effectively treating high ammonium-strength wastewater using anammox under IC limitation., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023. Published by Elsevier B.V.)

A better understanding of blue carbon (BC) sequestration can not only contribute to a better elucidation of global carbon cycle processes but can also lay the foundation for the incorporation of BC ecosystems into regional and global carbon offset schemes. In this study, the surface soils of seven plots along a landward to seaward distance gradient were analyzed for the concentrations and stocks of soil organic carbon (SOC), soil inorganic carbon (SIC), dissolved organic carbon (DOC), and dissolved inorganic carbon (DIC), as well as soil physical (bulk density, texture, moisture), chemical (pH, electrical conductivity), and microbiological (phospholipid fatty acid) properties in the coastal wetlands. Correlation, variation partition and random forest (RF) analyses were used to identify key variables correlating with BC fraction distribution patterns. The results suggested that SIC, DIC, and DOC, exhibited similar landward-increasing trends but the driving factors were distinct from each other. Based on correlation and RF analysis, both SIC and DIC were closely related to soil moisture and clay contents, but microbial indicators of arbuscular mycorrhizal fungi and actinomycete, were found to be associated with SIC, and abiotic properties played less important but still substantial roles in predicting DIC dynamics. In contrast with the other three investigated BC fractions, SOC showed a slight tendency toward enrichment in the seaward direction, and SIC was identified as the main driving factor. DOC showed no significant correlations with the other BC fractions, and its variation could not be explained well by the selected edaphic parameters. The soils in the YRD's tidal Suaeda salsa salt marshes showed a significant negative coupled SOC-SIC correlation, which was potentially related to divergent sedimentary processes and potential biotransformation between SOC and SIC. These results highlight the importance of integrating multiple BC fractions and their interactions into attempts to explore key mechanisms of BC cycling., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 Elsevier Ltd. All rights reserved.)

The allocation dynamics of soil carbon pools during soil development and land use are the key to revealing the carbon cycle process. To clarify the distribution of the soil carbon pool and its change trend, a soil reclamation chronosequence (0 a, 60 a, 160 a, 280 a, 1 000 a, and 1 500 a reclamation) was established in a typical alluvial plain in the Lower Yangtze River, and the content and density of soil organic carbon (SOC), soil inorganic carbon (SIC), particulate organic carbon (POC), and mineral-associated organic carbon (MAOC), along with carbon sequestration potential (CSP) indicators of topsoil under different land use types were measured and analyzed. The results showed that after approximately 1 500 a reclamation, the SOC content developed from the Yangtze River alluvial deposits generally increased by 4.9% after the initial decline, whereas the SIC content decreased to 0.2% from 25.8% of the total carbon content due to its rapid leaching. The MAOC content was normally higher than that of POC, and MAOC was contributing 48.0%-79.7% of the SOC accumulation. In this region, the soil organic carbon density (SOCD) accounted for 57.4%-100% of the total carbon density, the soil carbon sequestration levels (CSL) ranged from 18.6% to 56.1%, and CSP under paddy-dryland rotation increased by 20.8% compared to that under dryland. The C/N ratio and total nitrogen content are key factors in explaining soil carbon accumulation processes, and the reclamation year plays an important role in evaluating soil carbon sequestration levels. After long-term utilization, the cultivated soil in the Yangtze River floodplain must be carefully managed through balanced fertilization to maintain soil productivity, promote the accumulation of SOC, and avoid the decline in soil carbon sequestration capacity.

Soil application of Ca- and Mg-rich silicates can capture and store atmospheric carbon dioxide as inorganic carbon but could also have the potential to stabilise soil organic matter (SOM). Synergies between these two processes have not been investigated. Here, we apply finely ground silicate rock mining residues (basalt and granite blend) to a loamy sand in a pot trial at a rate of 4% (equivalent to 50 t ha -1 ) and investigate the effects of a wheat plant and two watering regimes on soil carbon sequestration over the course of 6 months. Rock dust addition increased soil pH, electric conductivity, inorganic carbon content and soil-exchangeable Ca and Mg contents, as expected for weathering. However, it decreased exchangeable levels of micronutrients Mn and Zn, likely related to the elevated soil pH. Importantly, it increased mineral-associated organic matter by 22% due to the supply of secondary minerals and associated sites for SOM sorption. Additionally, in the nonplanted treatments, rock supply of Ca and Mg increased soil microaggregation that subsequently stabilised labile particulate organic matter as organic matter occluded in aggregates by 46%. Plants, however, reduced soil-exchangeable Mg and Ca contents and hence counteracted the silicate rock effect on microaggregates and carbon within. We suggest this cation loss might be attributed to plant exudates released to solubilise micronutrients and hence neutralise plant deficiencies. The effect of enhanced silicate rock weathering on SOM stabilisation could substantially boost its carbon sequestration potential., (© 2023 The Authors. Global Change Biology published by John Wiley & Sons Ltd.)

Protein phosphorylation via serine/threonine protein kinases (Spk) is a widespread mechanism to adjust cellular processes toward changing environmental conditions. To study their role(s) in cyanobacteria, we investigated a collection of 11 completely segregated spk mutants among the 12 annotated Spks in the model cyanobacterium Synechocystis sp. PCC 6803. Screening of the mutant collection revealed that especially the mutant defective in SpkB encoded by slr1697 showed clear deviations regarding carbon metabolism, that is, reduced growth rates at low CO 2 or in the presence of glucose, and different glycogen accumulation patterns compared to WT. Alterations in the proteome of ΔspkB indicated changes of the cell surface but also metabolic functions. A phospho-proteome analysis revealed the absence of any phosphorylation in two proteins, while decreased phosphorylation of the carboxysome-associated protein CcmM and increased phosphorylation of the allophycocyanin alpha subunit ApcA was detected in ΔspkB. Furthermore, the regulatory P II protein appeared less phosphorylated in the mutant compared to WT, which was verified in Western blot experiments, indicating a clearly delayed P II phosphorylation in cells shifted from nitrate-containing to nitrate-free medium. Our results indicate that SpkB is an important regulator in Synechocystis that is involved in phosphorylation of the P II protein and additional proteins., Competing Interests: Conflict of interest The authors declare no competing interests., (Copyright © 2023 The Authors. Published by Elsevier Inc. All rights reserved.)

Total organic carbon (TOC) analysis with accurate determination of particulate organic carbon (POC) content in suspended solids (SS) containing water is critical for evaluating the environmental impact of particulate organic pollutants in water and calculating the carbon cycle mass balance. TOC analysis is divided into the non-purgeable organic carbon (NPOC) and differential (known as TC-TIC) methods; although the selection of method is greatly affected by the sample matrix characteristics of SS, no studies have investigated this. This study quantitatively evaluates the effect of SS containing inorganic carbon (IC) and purgeable organic carbon (PuOC), as well as that of sample pretreatment, on the accuracy and precision of TOC measurement in both methods for various environmental water sample types (12 wastewater influents and effluents and 12 types of stream water). For influent and stream water with high SS, the TC-TIC method expressed 110-200 % higher TOC recovery than that for the NPOC method due to POC component losses in SS owing to its conversion into PuOC during sample pretreatment (using ultrasonic) and subsequent loss in the NPOC purging process. Correlation analysis confirmed that particulated organic matter (POM, mg/L) content in SS directly affected this difference (r > 0.74, p < 0.01, n = 24); for POC water samples (those containing >10 mg/L of POM) featuring purgeable dissolved organic matter, TC-TIC was appropriate in securing TOC measurement accuracy. In constrast, in effluent and stream water with low SS (i.e., < ∼5 mg/L) and high IC (> 70 %) contents, the TOC measurement ratios (TC-TIC/NPOC) of both methods were similar, between 0.96 and 1.08, suggesting that NPOC is appropriate for improving precision. Our results provide useful basic data to establish the most reliable TOC analysis method considering SS contents and its properties along with the matrix characteristics of the sample., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 Elsevier B.V. All rights reserved.)

Carbon dioxide (CO 2 ) emitted by human activities not only brings about a serious greenhouse effect but also accelerates global climate change. This has resulted in extreme climate hazards that can obstruct human development in the near future. Hence, there is an urgent need to achieve carbon neutrality by increasing negative emissions. The ocean plays a vital role in absorbing and sequestering CO 2 . Current research on marine carbon storage and sink enhancement mainly focuses on biological carbon sequestration using carbon sinks (macroalgae, shellfish, and fisheries). However, seawater inorganic carbon accounts for more than 95 % of the total carbon in marine carbon storage. Increasing total alkalinity at a constant dissolved inorganic carbon shifts the balance of existing seawater carbonate system and prompts a greater absorption of atmospheric CO 2 , thereby increasing the ocean's "carbon sink". This review explores two main mechanisms (i.e., enhanced weathering and ocean alkalinization) and materials (e.g., silicate rocks, metal oxides, and metal hydroxides) that regulate marine chemical carbon sink (MCCS). This work also compares MCCS with other terrestrial and marine carbon sinks and discusses the implementation of MCCS, including the following aspects: chemical reaction rate, cost, and possible ecological and environmental impacts., Competing Interests: Declaration of competing interest The authors declare no known competing financial interests or personal relationships that could have influenced the work reported in this study., (Copyright © 2023 Elsevier B.V. All rights reserved.)

In hardwater lakes, calcite precipitation is an important yet poorly understood process in the lacustrine carbon cycle, in which catchment-derived alkalinity (Alk) is both transformed and translocated. While the physico-chemical conditions supporting the supersaturation of water with respect to calcite are theoretically well described, the magnitude and conditions underlying calcite precipitation at fine temporal and spatial scales are poorly constrained. In this study, we used high frequency, depth-resolved (0-30 m) data collected over 18 months (June 2019 - November 2020) in the deeper basin of Lake Geneva to describe the dynamics of calcite precipitation fluxes at a fine temporal resolution (day to season) and to scale them to carbon fixation by primary production. Calcite precipitation occurred during the warm stratified periods when surface water CO 2 concentrations were below atmospheric equilibrium. Seasonally, the extent of Alk loss due to calcite precipitation (i.e., [30-42] g C m -2 ) depended upon the level of Alk in surface waters. Moreover, interannual variability in seasonal calcite precipitation depended on the duration of stratification, which determined the volume of the water layer susceptible to calcite precipitation. At finer timescales, calcite precipitation was characterized by marked daily variability with dynamics strongly related to that of planktonic autotrophic metabolism. Increasing daily calcite precipitation rates (i.e., maximum values 9 mmol C m -3 d -1 ) coincided with increasing net ecosystem production (NEP) during periods of enhanced water column stability. In these conditions, calcite precipitation could remove as much inorganic carbon from the productive layers as NEP. This study provides mechanistic insights into the conditions driving pelagic calcite precipitation, and quantifies its essential contribution to the coupling of organic and inorganic carbon cycling in lakes., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2022 The Authors. Published by Elsevier B.V. All rights reserved.)

Algae-bacteria systems are used widely in wastewater treatment. N-hexanoyl-L-homoserine lactone (AHL) plays an important role in algal-bacteria communication. However, little study has been conducted on the ability of AHLs to regulate algal metabolism and the carbon fixation ability, especially in algae-bacteria system. In this study, we used the Microcystis aeruginosa + Staphylococcus ureilyticus strain as a algae-bacteria system. The results showed that 10 ng/L C 6 -HSL effectively increased the chlorophyll-a (Chl-a) concentration and carbon fixation enzyme activities in the algae-bacteria group and algae group, in which Chl-a, carbonic anhydrase activity, and Rubisco enzyme increased by 40% and 21%, 56.4% and 137.65%, and 66.6% and 10.2%, respectively, in the algae-bacteria group and algae group, respectively. The carbon dioxide concentration mechanism (CCM) model showed that C 6 -HSL increased the carbon fixation rate of the algae-bacteria group by increasing the CO 2 transport rate in the water and the intracellular CO 2 concentration. Furthermore, the addition of C 6 -HSL promoted the synthesis and secretion of the organic matter of algae, which provided biogenic substances for bacteria in the system. This influenced the metabolic pathways and products of bacteria and finally fed back to the algae. This study provided a strategy to enhance the carbon fixation rate of algae-bacteria consortium based on quorum sensing.

Sulfur-based autotrophic bioremediation is recognized as an environmentally-friendly and effective method for the treatment of Cr(VI) in groundwater. However, inorganic carbon (IC), especially IC-rich solid kitchen waste, has rarely been reported as an important factor in the autotrophic process. In China, kitchen waste containing IC is generated in large quantities, and in combination with Cr(VI) autotrophic treatment technology in groundwater can achieve a win-win situation. Herein, the efficiency of Cr(VI)-bioreduction coupling solid inorganic carbon (SIC) (e.g. marble, egg shell, oyster shell, and NSAD synthetic material) and liquid inorganic carbon (LIC) was compared for the first time. After 18 d incubation, there were significant differences in Cr(VI) reduction efficiency and microbial community between SIC-bioreactors and LIC-bioreactors. Higher electron transfer activity, greater bioavailability of organics, and multiple Cr(VI) reductases were detected in SIC-biosystems, which effectively promoted Cr(VI) energy metabolism and enzyme-mediated biological reduction. High-throughput 16S rRNA gene sequencing reveled multiple cooperative mechanism in different substrate biosystems. This study not only advances the understanding of SIC coupled with Cr(VI) autotrophic bioreduction, but also provides new insights for the treatment of solid kitchen waste and groundwater bioremediation., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2022 Elsevier B.V. All rights reserved.)

The significance of inorganic carbon (IC) for anaerobic ammonium oxidation (anammox) bacteria has been verified. However, the regulation of metabolic pathways under IC stress is not clear, limiting the optimization of IC supply. In this study, the regulatory pathways at IC concentration of 5-150 mg/L were explored to achieve optimal control of IC. The results show that the changes of metabolic pathway under IC stress determined anammox characteristics. At IC concentration of 5 mg/L, the anammox activity distinctly decreased due to the guanosine tetraphosphate (ppGpp) -mediated regulation under IC limitation. With less than 15 mg/L of IC, the decrease of carbon fixation limited the biosynthesis of gluconeogenesis and amino acids, causing the decline of extracellular polymeric substance synthesis. With more than 50 mg/L of IC, the improvement of purine and pyrimidine metabolism enhanced the electron transport capacity and growth potential of anammox bacteria. This study provides metabolic insights into IC influence on anammox consortia and a novel method of IC concentration optimization using metabolomics analysis., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2022 Elsevier B.V. All rights reserved.)

Global change impacts important environmental drivers for pelagic gross primary production (GPP) in northern lakes, such as temperature, light, nutrient, and inorganic carbon availability. Separate and/or synergistic impacts of these environmental drivers on pelagic GPP remain largely unresolved. Here, we assess key drivers of pelagic GPP by combining detailed depth profiles of summer pelagic GPP with environmental and climatic data across 45 small and shallow lakes across northern Sweden (20 boreal, 6 subarctic, and 19 arctic lakes). We found that across lakes summer pelagic GPP was strongest associated with lake water temperatures, lake carbon dioxide (CO 2 ) concentrations impacted by lake water pH, and further moderated by dissolved organic carbon (DOC) concentrations influencing light and nutrient conditions. We further used this dataset to assess the extent of additional DOC-induced warming of epilimnia (here named internal warming), which was especially pronounced in shallow lakes (decreasing 0.96°C for every decreasing m in average lake depth) and increased with higher concentrations of DOC. Additionally, the total pools and relative proportion of dissolved inorganic carbon and DOC, further influenced pelagic GPP with drivers differing slightly among the boreal, subarctic and Arctic biomes. Our study provides novel insights in that global change affects pelagic GPP in northern lakes not only by modifying the organic carbon cycle and light and nutrient conditions, but also through modifications of inorganic carbon supply and temperature. Considering the large-scale impacts and similarities of global warming, browning and recovery from acidification of lakes at higher latitudes throughout the northern hemisphere, these changes are likely to operate on a global scale., (© 2022 The Authors. Global Change Biology published by John Wiley & Sons Ltd.)

Selective production of carboxylic acids (CAs) from mixed culture fermentation remains a difficult task in organic waste valorization. Herein, we developed a facile and sustainable carbon loop strategy to regulate the fermentation micro-environment and steer acidogenesis towards selective butyric acid production. This new ion substitution electrodialysis-anaerobic membrane bioreactor (ISED-AnMBR) integrated system demonstrated a high butyric acid production at 11.19 g/L with a mass fraction of 76.05%. In comparison, only 1.04 g/L with a mass fraction of 30.56% was observed in the uncoupled control reactor. The carbon recovery reached a maximum of 96.09% with the assistance of ISED. Inorganic carbon assimilation was believed to be an important contributor, which was verified by 13 C isotopic tracing. Microbial community structure shows the dominance of Clostridia (80.16%) in the unique micro-environment (e.g., pH 4.80-5.50) controlled by ISED, which is believed beneficial to the growth of such fermentative bacteria with main products of butyric acid and acetic acid. In addition, the emergence of chain elongators such as Clostridium sensu stricto 12 was observed to have a great influence on butyric acid production. This work provides a new approach to generate tailored longer chain carboxylic acids from organic waste with high titer thus contributing to a circular economy., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2022. Published by Elsevier Ltd.)

A comprehensive dataset of 138 surficial sediment samples retrieved from the shallow marine waters of six secondary compartments off the western coast of Victoria, Australia, is presented. Samples were collected between October 2018 and November 2020 at water depths ranging from four to 55 m using Shipek and Van Veen grabs. Sampling design targeted unconsolidated areas of the seafloor based on bathymetric and seafloor habitat data. Retrieved sediments were subsampled and subject to grain size analysis using a combination of dry sieving and laser diffraction methods, carbonate and organic matter content determination via Loss-on-Ignition, colour description using a Munsell chart, and roundness analysis using microscopic photography. This dataset, the most comprehensive surficial shallow water sedimentary record of the Otway Shelf, serves as a benchmark to understand sediment dynamics and conectivity along the coast, and can be used in environmental and engineering studies to support a range of management decisions., Competing Interests: The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. The authors declare the following financial interests/personal relationships which may be considered as potential competing interests:, (© 2022 The Authors. Published by Elsevier Inc.)

Copper can be beneficial or harmful to coral at environmentally relevant levels, making environmental monitoring a challenging. Membrane lipids make the cell a dynamic environment according to the circumstances; thus, the lipid profile should be indicative of an environmental/physiological state. To gain more insight into the copper effect on coral health and be a basis of biomonitoring, glycerophosphocholine profiling of coral exposed to microenriched copper levels was conducted in this study. The copper microenrichments resulted in a diacritical effect of decreasing carbonic anhydrase activity, following a supplementation effect, on coral lipid metabolism. Microdifferences in copper levels are critical to determine the coral metabolic state and were therefore included in this study. In addition, an excellent quantitative model correlating the coral lipid variation with the exposed copper levels or the induced physiological effect was obtained to demonstrate its performance for biomonitoring., (Copyright © 2022 Elsevier Ltd. All rights reserved.)

Due to an increasing CO 2 concentration leading to global warming, the techniques as carbon capture utilization and storage are currently critical issues. This study aimed to investigate a cultivation strategy using optimal inorganic carbon level, irradiance, and photoperiod for producing the highest biomass and photosynthesis pigment contents (chlorophyll and phycobiliprotein) in the macroalga Colaconema formosanum. The results revealed that adding 1 g L -1 carbon increases phycoerythrin ratio by 12.52-13.74% and decreases allophycocyanin by 10.4-9.57%. Optimal conditions can increase algal growth by 60%, providing 5-6 mg g -1 total phycobiliprotein and 650-680 µg g -1 total chlorophyll. The results in this study illustrate the sensitivity of photosynthesis pigment after treatment with carbon, and suggest a hypothesis explaining the mechanism. The results also provide a feasible use of carbon for high-value large-scale production of pigment in the macroalgae industry., (Copyright © 2022 Elsevier Ltd. All rights reserved.)

The usage of fossil fuels results in a high amount of greenhouse gas (GHG) emissions and renewable green energy requirements entail saving ecological balance. Therefore, microalgae cultivation is widespread as a suitable raw material to produce renewable and sustainable fuel. Mathematical models are useful tools for the estimation of different conditions of a system. In this study, mathematical models were developed for monitoring the cultivation of local species of microalgae based on the chlorophyll-a and biomass concentration. Coefficients that were calculated from the Monod kinetic model were μmax; 0.03 day -1 , K S , C i ; 0.53 mM with an R 2 value of 0.93 and from the Aiba inhibition kinetic model was μmax and K S , C i 1.48 day -1 and 0.08 mM with an R 2 value of 0,73. According to the literature, there was no model was developed for the determination of kinetic coefficients based on chlorophyll-a production due to the inorganic carbon consumption. While both growth and inhibition models were developed for the inorganic carbon consumption, chlorophyll-a concentration was used for the growth model and biomass concentration was used for the inhibition model which caused and directly affected by the decrease of light penetration. The maximum biomass and chlorophyll-a concentrations were found as 1.2 g/L and 27.8 mg/L respectively with 10.24 mg/L. day -1 nitrogen and 1.19 mg/L.day -1 phosphorus uptake rate., (Copyright © 2021. Published by Elsevier Ltd.)

As a promising wastewater treatment technology, little is known about whether the greenhouse gas CO 2 can be applied for microalgal-bacterial granular sludge (MBGS) process. This article applied CO 2 for improving MBGS process. It was found that the physical structure of MBGS with no CO 2 addition appeared to have a trend to be loose and disintegrated, with a sludge volume index at 5 min (SVI 5 ) of over 150 mL/g and an average pore size of 35 nm in 60 d operation. However, CO 2 could maintain the compact and integrated structure of MBGS with a SVI 5 lower than 50 mL/g and an average pore size ranging from 10 to 13 nm. CO 2 could enhance the production of extracellular polysaccharides and aromatic protein, thus favoring the granular stability of MBGS. CO 2 could change the aqueous environment, e.g. lowering the pH values, which resulted in different microbial communities as well as metabolic potentials of MBGS. As for the reactor performance, CO 2 could significantly improve the removals of organics and phosphorus, evidenced by the enhancement of genes encoding acetate-CoA ligase and ATPase, respectively. Although the mass ratio of algae to bacteria was elevated by CO 2 addition, the ammonia removal related enzymes of glutamate dehydrogenase and glutamine synthetase could be negatively and positively impacted by CO 2 , respectively. Mass balance analysis of carbon indicated that CO 2 could provide additional carbon source as well as enhance the buffering capacity for the MBGS system. Further estimations suggested that the MBGS process could achieve a carbon-negative objective for municipal wastewater treatment by supplying CO 2 as additional carbon source. Hence, CO 2 supply for MBGS process in municipal wastewater treatment can be deemed as a two-birds-one-stone strategy, i.e. maintaining the granular stability and eliminating the carbon emission. This article can advance our basic knowledge on MBGS process towards environment-sustainable wastewater treatment., (Copyright © 2021 Elsevier Ltd. All rights reserved.)

Herein, the effect of inorganic carbon (IC) limitation on the nitrogen removal performance of the single-stage reactor containing nitritation and anammox gel carriers was investigated. As a result of a continuous feeding test, the effluent ammonium concentration increased as the IC concentration decreased, indicating the deterioration of nitritation activity, not anammox. Furthermore, the sensitivity of IC to anammox and nitritation activity was investigated in anammox and nitritation reactors, respectively. Consequently, the relationship between the effluent IC concentration and nitritation rate was well described using the Michaelis-Menten equation. The apparent K m value of nitritation was calculated as 4.4 mg-C L -1 . In anammox reactor, it was calculated as 1.7 mg-C L -1 . These results revealed that the affinity of nitritation gel carriers to IC was lower than that of anammox, supporting that nitritation activity was easily deactivated by decrease in the IC concentration rather than anammox. Microbial community analysis revealed that Nitrosomonas europaea and Candidatus Jettenia asiatica were the dominant species of ammonium-oxidizing and anammox bacteria., (Copyright © 2021 The Society for Biotechnology, Japan. Published by Elsevier B.V. All rights reserved.)

The uptake of inorganic carbon in cyanobacteria is facilitated by an energetically intensive CO 2 -concentrating mechanism (CCM). This includes specialized Type-1 NDH complexes that function to couple photosynthetic redox energy to CO 2 hydration forming the bicarbonate that accumulates to high cytoplasmic concentrations during the operation of the CCM, required for effective carbon fixation. Here we used a Synechococcus PCC7942 expression system to investigate the role of conserved histidine and cysteine residues in the CupB (also designated, ChpX) protein, which has been hypothesized to participate in a vectoral CO 2 hydration reaction near the interface between CupB protein and the proton-pumping subunits of the NDH-1 complex. A homology model has been constructed and most of the targeted conserved residues are in the vicinity of a Zn ion modeled to form the catalytic site of deprotonation and CO 2 hydration. Growth and CO 2 uptake assays show that the most severe defects in activity among the targeted residues are due to a substitution of the predicted Zn ligand, CupB-His86. Mutations at other sites produced intermediate effects. Proteomic analysis revealed that some amino acid substitution mutations of CupB caused the induction of bicarbonate uptake proteins to a greater extent than complete deletion of CupB, despite growth under CO 2 -enriched conditions. The results are discussed in terms of hypotheses on the catalytic function of this unusual enzyme., (Copyright © 2021 Elsevier B.V. All rights reserved.)

Norwegian fjords have been recently recognized as hot spots for carbon burial due to the large amounts of terrestrial organic matter delivered to fjord sediments, as well as the high sediment accumulation rates. Here, we present the first data on the contribution of benthic foraminiferal inorganic carbon to the sediments of three Norwegian fjords. Our study shows that calcareous foraminifera, which are among the most abundant calcifying organisms in the modern global oceans, can constitute between 15% and 33% of inorganic carbon accumulated in the sediments of the two studied southern Norwegian fjords (Raunefjorden and Hjeltefjorden). In a northern Norwegian fjord (Balsfjorden), the contribution of calcareous foraminifera to the inorganic carbon pool is smaller (<1%) than the one observed in southern fjords. We also found that the amount of foraminifera-derived carbon is primarily dependent on the species composition of the foraminifera community. Large calcareous foraminifera species, despite a lower number of individuals, constitute, on average, 13%-29% of the inorganic carbon in the two southern Norwegian fjords, while the contribution of small, highly abundant species does not exceed 4% of the inorganic carbon pools in the sediments. Calcareous foraminifera species that are indicative of dysoxic conditions have been found to have low inorganic carbon contents per specimen compared to other analysed similar-sized calcareous foraminifera species. This relationship most likely exists due to the thin test walls of these foraminifera species, which may facilitate gas exchange. The results of our case study suggest that the climate-driven formation of near-bottom low-oxygen zones may lead to the dominance of foraminifera associated with dysoxic conditions and, in consequence, to the decrease of foraminifera-derived inorganic carbon. However, to properly analyse the contribution of carbon from thin-walled foraminifera to the sedimentary carbon pool, further studies analysing a broader range of these species is needed., (© 2021 John Wiley & Sons Ltd.)

Nitrogen and phosphorous concentrations are widely considered to drive macrophyte assemblages in rivers. However, Dissolved Inorganic Carbon (DIC) - available for plants as CO 2 and HCO 3 - - is also of major relevance. Based on literature, we present a conceptual model on the interaction between algae, macrophytes, DIC, pH, light, N, P and the surface water and sedimental compartment. Analysing two separate datasets (i) on river physico-chemistry and chlorophyll-a, and (ii) on river physico-chemistry and macrophytes we quantify three connections within this concept: (1) the correlation of chlorophyll-a versus pH, (2) the correlation of TP versus chlorophyll-a and (3) the occurrence of HCO 3 -users and CO 2 -only-users among macrophytes along the DIC gradient. Chlorophyll-a correlated positively with pH (R-squared = 77%, p < .001) due to increased carbon dioxide uptake of phytoplankton. Surface water TP did not linearly correlate with chlorophyll-a concentrations. Obligate and optionally submerged macrophyte species that utilise HCO 3 - were separated from CO 2 -only-users by HCO 3 - concentrations, with an area under the curve (AUC) of 68% and 70% (both p < .001) between groups. Obligate and optionally submerged macrophyte assemblages only composed of HCO 3 -users and those exclusively composed of CO 2 -only-users showed an even stronger separation based on the HCO 3 - concentration, with both an AUC of 82% and 78% (both p < .001). Our results underline that DIC can greatly affect riverine macrophytes. However, absolute concentrations of HCO 3 - are less relevant, while the connection to pH is more important, reflecting CO 2 concentrations. River monitoring and management should consider the interaction between nutrients DIC, surface water and sedimental compartment as important factors affecting macrophyte occurrence, rather than solely focussing on surface water nutrients., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2021 Elsevier B.V. All rights reserved.)

This study intended to examine the influence of biochar application on soil carbon content under different tillage conditions. For this, an indoor incubation experiment was performed with treatments included wheat straw-derived biochar application (0, 5, and 20 g·kg -1 ) and soil with different tillage measures (ploughing and no-tillage). The effects of biochar addition on soil organic carbon (SOC), dissolved organic carbon (DOC), soil microbial biomass carbon (MBC), readily oxidized organic carbon (ROC), soil inorganic carbon (SIC), pH, water soluble calcium and magnesium, and soil CO 2 emissions were analyzed. The results showed that:① Compared with the control, the contents of SOC, ROC, DOC, and water soluble Ca and Mg increased by 20.3%-105.6%, 0.5%-36.0%, 0.8%-30.5%, 3.5%-42.3%, and 2.4%-75.2% in the no-tillage treatments, respectively; and the contents of SOC, ROC, DOC, water-soluble Ca and Mg increased by 29.2%-145.1%, 1.3%-63.9%, 2.4%-55.6%, 18.2%-89.8%, and 10.1%-150.5% in the ploughing treatment, respectively, under different dosage biochar amendments, and was enhanced with an increase in the biochar application amount. Cumulative CO 2 emissions were highest with biochar amendment at 5 g·kg -1 under the no-tillage soil condition; however, this increased with an increase in the biochar amount in the ploughing treatment. At the end of incubation experiment, the soil MBC content increased by 35.5%-45.7% compared with the control treatment; however, there was no significant effect on soil pH and SIC between the treatments. ② Compared with the ploughing treatment, the cumulative CO 2 emissions, SOC, ROC, DOC, MBC, and water-soluble Ca and Mg contents of the no tillage treatment increased by 34.2%-79.0%, 8.9%-45.5%, 28.2%-73.9%, 40.4%-78.4%, 0.2%-131.7%, 8.7%-39.8%, and 0.3%-61.0%, respectively, while soil pH and SIC decreased by 0.08-0.17 unit and 2.4%-13.9%, respectively, under the same biochar amendment treatments. Overall, the addition of biochar significantly increased soil organic carbon, active organic carbon, soil water soluble calcium and magnesium content, and soil cumulative CO 2 emissions, but no significant effect was observed on soil inorganic carbon content.

Cell size influences the rate at which phytoplankton assimilate dissolved inorganic carbon (DIC), but it is unclear whether volume-specific carbon uptake should be greater in smaller or larger cells. On the one hand, Fick's Law predicts smaller cells to have a superior diffusive CO 2 supply. On the other, larger cells may have greater scope to invest metabolic energy to upregulate active transport per unit area through CO 2 -concentrating mechanisms (CCMs). Previous studies have focused on among-species comparisons, which complicates disentangling the role of cell size from other covarying traits. In this study, we investigated the DIC assimilation of the green alga Dunaliella tertiolecta after using artificial selection to evolve a 9.3-fold difference in cell volume. We compared CO 2 affinity, external carbonic anhydrase (CA ext ), isotopic signatures (δ 13 C) and growth among size-selected lineages. Evolving cells to larger sizes led to an upregulation of CCMs that improved the DIC uptake of this species, with higher CO 2 affinity, higher CA ext and higher δ 13 C. Larger cells also achieved faster growth and higher maximum biovolume densities. We showed that evolutionary shifts in cell size can alter the efficiency of DIC uptake systems to influence the fitness of a phytoplankton species., (© 2020 The Authors New Phytologist © 2020 New Phytologist Foundation.)

The data on stress-related changes in the expression and activity of plant carbonic anhydrases (CAs) suggest that they are generally upregulated at moderate stress severity. This indicates probable involvement of CAs in adaptation to drought, high salinity, heat, high light, C i deficit, and excess bicarbonate. The changes in CA levels under cold stress are less studied and generally represented by the downregulation of CAs excepting βCA2. Excess Cd 2+ and deficit of Zn 2+ specifically reduce CA activity and reduce its synthesis. Probable roles of βCAs in stress adaptation include stomatal closure, ROS scavenging and partial compensation for decreased mesophyll CO 2 conductance. βCAs play contrasting roles in pathogen responses, interacting with phytohormone signaling networks. Their role can be either negative or positive, probably depending on the host-pathogen system, pathogen initial titer, and levels of ·NO and ROS. It is still not clear why CAs are suppressed under severe stress levels. It should be noted, that the role of βCAs in the facilitation of CO 2 diffusion and their involvement in redox signaling or ROS detoxication are potentially antagonistic, as they are inactivated by oxidation or nitrosylation. Interestingly, some chloroplastic βCAs may be relocated to the cytoplasm under stress conditions, but the physiological meaning of this effect remains to be studied.

Main Conclusion: β-carbonic anhydrases, which function in regulating plant growth, C/N status, and stomata number, showed functional redundancy and divergence in Physcomitrella patens. Carbonic anhydrases (CAs) catalyze the interconversion of CO 2 and HCO 3 - . Plants have three evolutionarily unrelated CA families: α-, β-, and γ-CAs. βCAs are abundant in plants and are involved in CO 2 assimilation, stress responses, and stomata formation. Recent studies of βCAs have mainly examined C 3 or C 4 plants, whereas their functions in non-vascular plants are mostly unknown. In this study, phylogenetic analysis revealed that the evolution of βCAs were conserved between subaerial green algae and bryophytes after terrestrialization event, and βCAs from some cyanobacteria might begin evolving for the adaptation of terrestrial environment/habitat. In addition, we investigated the physiological roles of βCAs in the basal land plant Physcomitrella patens. High PpβCA expression levels in different tissues suggest that PpβCAs play important roles in development in P. patens. Plants treated with 1-10 mM NaHCO 3 had higher fresh and dry weight, PpβCA expression, total CA activity, and photosynthetic yield (Fv/Fm) compared with water-treated plants. However, treatment with 10 mM NaHCO 3 influenced the C/N status. Further study of six Ppβca single-gene mutants revealed that PpβCAs have functional redundancy and divergence in regulating the C/N ratio of plants and stomatal formation. This study provides new insight into the physiological roles of βCAs in basal land plants.

Global change includes invasion by exotic (nonnative) plant species and altered precipitation patterns, and these factors may affect terrestrial carbon (C) storage. We measured soil C changes in experimental mixtures of all exotic or all native grassland plant species under two levels of summer drought stress (0 and +128 mm). After 8 yr, soils were sampled in 10-cm increments to 100-cm depth to determine if soil C differed among treatments in deeper soils. Total soil C (organic + inorganic) content was significantly higher under native than exotic plantings, and differences increased with depth. Surprisingly, differences after 8 yr in C were due to carbonate and not organic C fractions, where carbonate was ~250 g C/m 2 lower to 1-m soil depth under exotic than native plantings. Our results indicate that soil carbonate is an active pool and can respond to differences in plant species traits over timescales of years. Significant losses of inorganic C might be avoided by conserving native grasslands in subhumid ecosystems., (© 2020 by the Ecological Society of America.)

In response to high CO 2 environmental variability, green algae, such as Chlamydomonas reinhardtii, have evolved multiple physiological states dictated by external CO 2 concentration. Genetic and physiological studies demonstrated that at least three CO 2 physiological states, a high CO 2 (0.5-5% CO 2 ), a low CO 2 (0.03-0.4% CO 2 ) and a very low CO 2 (< 0.02% CO 2 ) state, exist in Chlamydomonas. To acclimate in the low and very low CO 2 states, Chlamydomonas induces a sophisticated strategy known as a CO 2 -concentrating mechanism (CCM) that enables proliferation and survival in these unfavorable CO 2 environments. Active uptake of C i from the environment is a fundamental aspect in the Chlamydomonas CCM, and consists of CO 2 and HCO 3 - uptake systems that play distinct roles in low and very low CO 2 acclimation states. LCI1, a putative plasma membrane C i transporter, has been linked through conditional overexpression to active C i uptake. However, both the role of LCI1 in various CO 2 acclimation states and the species of C i , HCO 3 - or CO 2 , that LCI1 transports remain obscure. Here we report the impact of an LCI1 loss-of-function mutant on growth and photosynthesis in different genetic backgrounds at multiple pH values. These studies show that LCI1 appears to be associated with active CO 2 uptake in low CO 2 , especially above air-level CO 2 , and that any LCI1 role in very low CO 2 is minimal., (© 2020 Society for Experimental Biology and John Wiley & Sons Ltd.)

This study illustrated the growth of Scenedesmus obliquus and recycle of nutrients in wastewater combined with inorganic carbon under autotrophic conditions. Scenedesmus obliquus was cultivated under different conditions by adding sodium carbonate (Na 2 CO 3 ) at 15-40 mg l -1 separately in wastewater containing high nitrogen and phosphorus content. The growth characteristics of S. obliquus , pH and dissolved inorganic carbon (DIC) changes of microalgae liquid, the recycle rate of ammonia and phosphorus and lipid content were determined. The changes of pH and DIC showed that S. obliquus could use Na 2 CO 3 to grow, with lipid contents of 18-25%. Among all Na 2 CO 3 concentrations, 20 mg l -1 was the optimum, of which S. obliquus had the highest NH 3 -N recycle of 52% and P O 4 3 - P recycle of 67%. By the 14th day, its biomass production also reaches the maximum of 0.21 g l -1 . However, inorganic carbon fixation rate was inversely proportional to its concentration. Moreover, the biomass was in positive correlation with the Na 2 CO 3 concentration except 20 mg l -1 , which provided a possibility that S. obliquus could be acclimatized to adjust to high concentrations of inorganic carbon to promote biomass accumulation and recycle of nutrients., Competing Interests: There are no competing interests to declare, (© 2020 The Authors.)

Current algal-bacterial consortia require high hydraulic retention times (HRTs, 2-10 days) to efficiently remove pollutants from domestic wastewaters. A novel algal-bacterial biofilm reactor was developed for a much lower HRT. The results showed that an HRT of 12 h ensured 90% removal of organic matter and ammonium, and phosphate removal was approximately 30%. Decreasing the HRT to 8 h significantly deteriorated the reactor's pollutant removal efficiencies and increasing the HRT to 24 h did not improve these efficiencies. Illumination, which was light source for algae, was provided by a LED light. Activity tests showed that organic matter and ammonium removal rates resulting from illumination were 70% and 50%, respectively, of the rates when dissolved oxygen concentration was maintained at 2 mg/L. Chemical oxygen demand (COD) removal rates resulted from illumination and aeration were 18.63 and 25.38 mg COD/L.h, respectively. The phosphate removal rate was 0.26 and 0.43 mg/L.h when illumination and aeration were applied, respectively. The ammonium removal rates were approximately 10,390 and 5000 mg [Formula: see text] when the reactor was aerated or illuminated, respectively. These two rates were significantly higher than reported nitrification rates. Moreover, the percentage of Oscillatoria sp. increased from below 10% to over 90% under the applied organic load and temperature, while the percentage of fast growing algae, Chlorella , chroococcus sp and Scenedesmus sp., decreased from over 90% to below 10%. These results showed that an algal-bacterial biofilm reactor with a low reactor footprint was developed.

The aim of this study was to investigate the PHB and glycogen accumulation dynamics in two photobioreactors inoculated with different monocultures of wastewater-borne cyanobacteria, using a three-stage feeding strategy (growth phase, feast-famine phase and feast phase). Two cyanobacterial monocultures containing members of Synechocystis sp. or Synechococcus sp. were collected from treated wastewater and inoculated in lab-scale photobioreactors to evaluate the PHB and glycogen accumulation. A third photobioreactor with a complex microbial community grown in real wastewater was also set up. During each experimental phase different concentrations of inorganic carbon were applied to the cultures, these shifts allowed to discern the accumulation mechanism of carbon storage polymers (PHB and glycogen) in cyanobacteria. Conversion of one into the other was directly related to the carbon content. The highest PHB and glycogen contents (5.04% dcw and 69% dcw , respectively) were achieved for Synechocystis sp., (Copyright © 2019 Elsevier Ltd. All rights reserved.)

Rising concentrations of atmospheric CO 2 results in higher equilibrium concentrations of dissolved CO 2 in natural waters, with corresponding increases in hydrogen ion and bicarbonate concentrations and decreases in hydroxyl ion and carbonate concentrations. Superimposed on these climate change effects is the dynamic nature of carbon cycling in coastal zones, which can lead to seasonal and diel changes in pH and CO 2 concentrations that can exceed changes expected for open ocean ecosystems by the end of the century. Among harmful algae, i.e. some species and/or strains of Cyanobacteria, Dinophyceae, Prymnesiophyceae, Bacillariophyceae, and Ulvophyceae, the occurrence of a CO 2 concentrating mechanisms (CCMs) is the most frequent mechanism of inorganic carbon acquisition in natural waters in equilibrium with the present atmosphere (400 μmol CO 2  mol -1 total gas), with varying phenotypic modification of the CCM. No data on CCMs are available for Raphidophyceae or the brown tide Pelagophyceae. Several HAB species and/or strains respond to increased CO 2 concentrations with increases in growth rate and/or cellular toxin content, however, others are unaffected. Beyond the effects of altered C concentrations and speciation on HABs, changes in pH in natural waters are likely to have profound effects on algal physiology. This review outlines the implications of changes in inorganic cycling for HABs in coastal zones, and reviews the knowns and unknowns with regard to how HABs can be expected to ocean acidification. We further point to the large regions of uncertainty with regard to this evolving field., (Copyright © 2019 Elsevier B.V. All rights reserved.)

Inorganic carbon is an important carbon source of autotrophic bacteria, e.g., ammonia-oxidizing bacteria. Ammonia-oxidizing bacteria are chemoautotrophic bacteria with carbon sequestration capacity. Experiments were performed on partial nitrification sludge with different influent matrices, and optimal experimental operational conditions were established. The carbon fixation pathway of ammonia-oxidizing sludge was determined via 13 C isotope tracers and qPCR. The denitrification effect was better when the NH 4 + -N, HCO 3 - , Ca 2+ , Mg 2+ , and microbial accelerant concentrations were 15, 250, 113, 100 and 1 mL/L, respectively. The nitrite accumulation rate reached 96.95%. 13 C isotope tracing showed that 13 C abundance in sludge increased significantly. The results showed that IC added into the influent participated in the carbon metabolism of microorganisms. The functional gene cbbL, which follows the Calvin cycle carbon sequestration pathway, was identified in the ammonia-oxidizing bacteria, and the effect of influent NH 4 + -N on the gene abundance was greater than that of other substrates., (Copyright © 2019 Elsevier Ltd. All rights reserved.)

With different inorganic carbon (IC) doses, nitrogen removal performance of marine anammox bacteria (MAB) treating nitrogen-rich saline wastewater was investigated in a sequencing batch reactor. Ammonium removal efficiency (ARE) was above 99% at 108-3600 mg/L IC, which indicated MAB had a good tolerance to high IC dose. When IC was 108-1200 mg/L, ARE reached 90% within 2.5 h. MAB activity was greatly promoted by providing adequate IC. Besides, the maximal substrate conversion rate (3.4 kg/(m 3  d)) was achieved at 180 mg/L IC. Both the modified Logistic and Boltzmann models were appropriate to describe nitrogen removal at low IC doses, while the modified Gompertz model was more accurate at high IC doses. Calcium carbonate crystal was formed on the surface of MAB granule at high IC doses, which resulted in a significant deterioration of nitrogen removal performance., (Copyright © 2019 Elsevier Ltd. All rights reserved.)

Anolyte acidification is inevitable in the operation of buffer-free microbial fuel cells (MFCs), which restricts the proliferation and metabolism of electroactive bacteria, and results in electric-power deterioration. The anodic metabolic end-products, inorganic carbons (IC), which are composed of H 2 CO 3 (dissolved CO 2 ), HCO 3 - , and CO 3 2- , are ideal endogenous buffers, whereas the naturally accumulated IC are far from enough to prevent anolyte acidification. In this work, different volume ratios of the anolytes (10%, 30%, and 50%) were recycled to increase the IC concentrations of the single-chamber air-cathode buffer-free MFCs. Under anolyte recycling running mode, IC accumulation agreed with the SGompertz model and the fitting IC-asymptotic concentrations (IC AC ) grew exponentially to 18.5 mM, 24.4 mM, and 32.8 mM as the anolyte recycling ratio increased from 10% to 30% and 50%. Self-buffering running can be realized when the anolyte recycling ratio exceeds 50% for the MFC feeding on 1 g·L -1 of acetate. The electric power for the 50% recycling scenario increased from the baseline control of 272.4 mW·m -2 to 628.5 mW·m -2 . The coulombic efficiency (CE) was also apparently improved. This paper for the first time clarifies the accumulation law of endogenous IC buffers under anolyte partially recycling mode and their self-buffering effects., (Copyright © 2019 Elsevier B.V. All rights reserved.)

The fluted giant clam, Tridacna squamosa, lives in symbiosis with photosynthetic zooxanthellae, and can engage in light-enhanced growth and shell formation. Light-enhanced shell formation necessitates the elimination of excess H + from the extrapallial fluid adjacent to the shell. This study aimed to clone Na + /H + Exchanger (NHE) from the whitish inner mantle adjacent to the extrapallial fluid of T. squamosa, to determine its cellular and subcellular localization, and to evaluate the effect of light exposure on its mRNA expression level and protein abundance therein. The complete coding cDNA sequence of NHE obtained was identified as a homolog of beta NHE (βNHE-like). It consisted of 2925 bp, encoding for a polypeptide of 974 amino acids and 107.1 kDa, and was expressed predominantly in the inner mantle. There, βNHE-like was localized in the apical membrane of the seawater-facing epithelium by immunofluorescence microscopy. After exposure to light for 12 h, the seawater-facing epithelium of the inner mantle displayed consistently stronger immunostaining than that of the control exposed to 12 h of darkness. Western blotting confirmed that light exposure significantly enhanced the protein abundance of βNHE-like in the inner mantle. These results denote that some of the excess H + generated during light-enhanced shell formation can be excreted through the light-dependent βNHE-like of the seawater-facing epithelium to minimize the impact on the whole-body pH. Importantly, the excreted H + could dehydrate exogenous HCO 3 , and facilitate the absorption of inorganic carbon through the seawater-facing epithelium dedicated for light-enhanced shell formation due to its close proximity with the shell-facing epithelium. NUCLEOTIDE SYMBOL COMBINATIONS: Pairs: R = A/G; W = A/T; Y = C/T. Triples: D = A/G/T.- , and facilitate the absorption of inorganic carbon through the seawater-facing epithelium dedicated for light-enhanced shell formation due to its close proximity with the shell-facing epithelium. NUCLEOTIDE SYMBOL COMBINATIONS: Pairs: R = A/G; W = A/T; Y = C/T. Triples: D = A/G/T., (Copyright © 2019 Elsevier B.V. All rights reserved.)

This paper investigates a mathematical model of competition between two species for inorganic carbon and light in a well-mixed water column. The population growth of the species depends on the consumption of two substitutable forms of inorganic carbon, "CO2" (dissolved CO2 and carbonic acid) and "CARB" (bicarbonate and carbonate ions), which are stored internally. Besides, uptake rates also includes self-shading by the phytoplankton population, that is, an increase in population density will reduce light available for photosynthesis, and thereby reducing further carbon assimilation and population growth. We also incorporate the fact that carbon is lost by respiration, and the respiration rate is assumed to be proportional to the size of the transient carbon pool. Then we study the extinction and persistence of a single-species system. Finally, we show that coexistence of the two-species system is possible, depending on parameter values, and both persistence of one population.

Water scarcity in arid, semiarid and dry regions is a limiting factor for the development of sustainable agriculture. As a consequence, the adoption of new strategies such as regulated deficit irrigation (RDI) to reduce water and energy consumption will be essential. Decreases in irrigation water content may also have positive effects on soil C cycle. Thus, an experiment was setup in three woody crop orchards during two years, with the objective of assessing if RDI can reduce soil CO 2 and N 2 O emissions, modify soil inorganic C and organic C quality and stability and affect soil aggregation. Soil CO 2 and N 2 O emissions were measured every two weeks while soil samplings were carried out every three months. Results indicated that decreases in soil moisture by RDI implementation were related to significant decreases in CO 2 emissions in all crops. RDI contributed to an average decrease, compared with full irrigation, of 1088-1664 g CO 2  m -2 in the experimental period. Furthermore, CO 2 emission was negatively correlated with inorganic C, suggesting the protective effect of soil carbonates towards organic matter. RDI also contributed to significantly decrease soil N 2 O emissions. However, N 2 O emission patterns did not directly follow soil moisture patterns and were constant in the experimental period. RDI contributed to an average decrease, compared with full irrigation, of 90-409 mg N 2 O m -2 . No physicochemical property was significantly affected by irrigation regime. Although microbial biomass was not significantly affected by RDI, β-glucosidase activity was significantly higher under full irrigation during the warm seasons, with significant positive correlation with CO 2 emissions. This seems to suggest that a significant fraction of CO 2 emitted from soil derives from organic matter degradation, which is limited with low water content. So, RDI could contribute to promote soil C sequestration by reduced greenhouse gas emissions, with no negative effects on soil structure at short-term., (Copyright © 2018 Elsevier B.V. All rights reserved.)

Stable nitritation for low-strength ammonium wastewater was the key obstacle for cost-effective and low-carbon biological nitrogen removal. A zeolite biological fixed bed (ZBFB) and an anoxic sequencing batch reactor (ASBR) were successfully applied for achieving nitritation-denitrification of low-strength ammonium wastewater by adsorption, biological desorption and denitrification. Based on free ammonia inhibition on biofilm, stable nitrite accumulation could be realized with suitable operational time and aeration in biological desorption. During cycle operation, adsorption effluent NH 4 + -N kept at 3.0-4.0 mg/L, biological desorption effluent NO 2 - -N maintained at 226.8-243.2 mg/L with average nitrite accumulation ratio of 97.18%, and nitrite removal rate was about 0.628-0.672 kg NO 2 - -N·m -3 ·day -1 , revealing obvious feasibility of ZBFB and ASBR for low-strength ammonium wastewater treatment. High-throughput sequencing analysis results further presented significant microbial community variations happened after cycle operation, with ammonia oxidizing bacteria enrichment and nitrite oxidizing bacteria inhibition in ZBFB and dominance of denitrifiers in ASBR., (Copyright © 2018 Elsevier Ltd. All rights reserved.)

The application of nitrogen (N) fertilizer results in decreases of soil pH, but its effects on CO 2 emission from soils with different inorganic carbon contents remain unclear. An closed-jar incubation experiment was conducted to examine the effects of N fertilizer and nitrification inhibitor (DCD) on soil pH and CO 2 emissions from three soil types with different contents of soil inorganic carbon (SIC), including paddy soil (PS), lime concretion black soil (CS), and eum-orthic anthrosols (AS). There were three treatments for each soil type, including control (N 0 ), 0.2 g·kg -1 N fertilizer (N 0.2 ), and its combination with DCD (N 0.2+DCD ). Soil pH, contents of mineral N (NH 4 + , NO 3 - ), and CO 2 emissions were measured. The results showed that N fertilizer addition significantly reduced soil pH and increased soil CO 2 cumulative emissions in each type of soil. Compared to control, the CO 2 cumulative emissions after 49 days incubation from the three soil types were enhanced by 39.4%, 23.4%, and 71.8% for PS, CS, AS soils, respectively. The soil pH of N 0.2+DCD for three soil types were significantly higher than N 0.2 after 49 days incubation, indicating that DCD application inhibited soil nitrification process. There were no significant differences in the mean CO 2 cumulative emissions of PS and CS soils between N 0.2 and N 0.2+DCD emissions from AS soil by 12.5%. Soil inorganic carbon can effectively buffer soil acidification caused by N fertilizer addition. The CO 0.2+DCD emission in calcareous soil following N addition is not only derived from the mineralization of soil organic carbon, but also from the dissolution of inorganic carbon. Given the large differences in soil inorganic carbon content in different regions of China, the problem of soil acidification and soil inorganic carbon pool consumption caused by long-term large amount of N fertilizer inputs deserve more attention.2 emissions from AS soil by 12.5%. Soil inorganic carbon can effectively buffer soil acidification caused by N fertilizer addition. The CO 2 emission in calcareous soil following N addition is not only derived from the mineralization of soil organic carbon, but also from the dissolution of inorganic carbon. Given the large differences in soil inorganic carbon content in different regions of China, the problem of soil acidification and soil inorganic carbon pool consumption caused by long-term large amount of N fertilizer inputs deserve more attention.

Nontuberculous mycobacteria (NTM) are widely distributed in the environment. On one hand, they are opportunistic pathogens for humans and animals, and on the other hand, they are effective in biodegradation of some persistent pollutants. Following the recently recorded large abundance of NTM in extreme geothermal environments, the aim of the study was to ascertain the occurrence of NTM in the extreme environment of the water zone of the Hranice Abyss (HA). The HA mineral water is acidic, with large concentrations of free CO 2 , and bacterial slimes creating characteristic mucilaginous formations. Both culture and molecular methods were used to compare the mycobacterial diversity across the linked but distinct ecosystems of HA and the adjacent Zbrašov Aragonite Caves (ZAC) with consideration of their pathogenic relevance. Six slowly growing NTM species (M. arupense, M. avium, M. florentinum, M. gordonae, M. intracellulare) and two rapidly growing NTM species (M. mucogenicum, M. sediminis) were identified in the water and in the dry zones at both sites. Proteobacteria were dominant in all the samples from both the HA and the ZAC. The bacterial microbiomes of the HA mineral water and HA slime were similar, but both differed from the microbiome in the ZAC mineral water. Actinobacteria, a phylum containing mycobacteria, was identified in all the samples at low proportional abundance. The majority of the detected NTM species belong among environmental opportunistic pathogens.

In this study, the effects of the ammonium loading rate (ALR) and inorganic carbon loading rate (ILR) on the nitrification performance and composition of a nitrifying bacterial community were investigated in a moving bed biofilm reactor, using poly(vinyl alcohol) (PVA) sponge cubes as a supporting carrier. Between the two ALRs of 0.36 and 2.16 kg-N m -1 d -1 , stable partial nitritation was achieved at the higher ALR. Inorganic carbon was dosed at high levels: 33.1, 22.0, 16.4, 11.0, and 5.4 times the theoretical amount. Nonetheless, nitrification efficiency was not affected by the ILR at the two ALRs. Quantitative PCR analysis of ammonia- and nitrite-oxidizing bacteria revealed that ALR is an important determinant of partial nitritation by accumulating ammonia-oxidizing bacteria in the nitrification system. In comparison, two nitrite-oxidizing bacterial genera (Nitrobacter and Nitrospira) showed almost the same relative abundance at various ALRs and ILRs. Terminal restriction fragment length polymorphism targeting the gene of ammonia monooxygenase subunit A revealed that Nitrosomonas europaea dominated under all conditions.

Even though nitrification/denitrification is a robust technology to remove nitrogen from sewage, economic incentives drive its future replacement by shortcut nitrogen removal processes. The latter necessitates high potential activity ratios of ammonia oxidizing to nitrite oxidizing bacteria (rAOB/rNOB). The goal of this study was to identify which wastewater and process parameters can govern this in reality. Two sewage treatment plants (STP) were chosen based on their inverse rAOB/rNOB values (at 20 °C): 0.6 for Blue Plains (BP, Washington DC, US) and 1.6 for Nieuwveer (NV, Breda, NL). Disproportional and dissimilar relationships between AOB or NOB relative abundances and respective activities pointed towards differences in community and growth/activity limiting parameters. The AOB communities showed to be particularly different. Temperature had no discriminatory effect on the nitrifiers' activities, with similar Arrhenius temperature dependences (Θ AOB  = 1.10, Θ NOB  = 1.06-1.07). To uncouple the temperature effect from potential limitations like inorganic carbon, phosphorus and nitrogen, an add-on mechanistic methodology based on kinetic modelling was developed. Results suggest that BP's AOB activity was limited by the concentration of inorganic carbon (not by residual N and P), while NOB experienced less limitation from this. For NV, the sludge-specific nitrogen loading rate seemed to be the most prevalent factor limiting AOB and NOB activities. Altogether, this study shows that bottom-up mechanistic modelling can identify parameters that influence the nitrification performance. Increasing inorganic carbon in BP could invert its rAOB/rNOB value, facilitating its transition to shortcut nitrogen removal., (Copyright © 2017 Elsevier Ltd. All rights reserved.)

While traditional laboratory methods of determining soil organic carbon (SOC) content are generally simple, this becomes more challenging when carbonates are present in the soil; such is commonly found in semi-arid areas. Additionally, soil inorganic carbon (SIC) content itself is difficult to determine. This study uses visible near infrared (VisNIR) spectra to predict SOC and SIC contents of samples, and the impact of including soil pH and soil total carbon (STC) data as predictor variables was evaluated. The results indicated that combining available soil pH and STC content data with VisNIR spectra dramatically improved prediction accuracy of the Cubist models. Using the full suite of predictor variables, Cubist models trained on the calibration dataset (75%) could predict the validation dataset (25%) for SOC content with a Lin's concordance correlation coefficient (LCCC) of 0.94, and an LCCC of 0.83 for SIC content. This is compared to an LCCC of 0.81 and 0.35 for SOC and SIC content, respectively, when no ancillary soil data was included with VisNIR spectra as predictor variables. These results suggest that there may be promise for using other readily available soil data in combination with VisNIR spectra to improve the predictions of different soil properties. •It can be laborious and expensive to measure soil organic and inorganic carbon content with traditional laboratory methods, and there has been recent focus on using spectroscopic techniques to overcome this.•This study demonstrates that combining ancillary soil data (pH and total carbon content) with these spectroscopic techniques can considerably improve predictions of SOC and SIC content.

Good start-up and performance are essential for the completely autotrophic nitrogen removal over nitrite (CANON) process, and inorganic carbon (IC) is also important for this process. In this study, a lab-scale submerged aerated biological filter (SABF) was adopted for the CANON process. A 16S rRNA gene high-throughput sequencing analysis showed that the phyla Proteobacteria and Planctomycetes were the dominant microorganisms and that the genus Candidatus Brocadia functioned as the nitrogen remover. The effect of IC on the nitrogen removal was analyzed. The results showed that the optimum concentration ratio of IC to nitrogen (IC/N) was 1.2, which produced the highest average ammonium nitrogen removal rate (ANR) and total nitrogen removal rate (TNR) values of 95.5% and 80.3%, respectively. The average AOB and AnAOB activities were 2.45 mg·L -1 ·h -1 and 3.57 mg·L -1 ·h -1 , respectively. This research could promote the nitrogen removal ability of the CANON process with a SABF in the future., (Copyright © 2018 Elsevier Ltd. All rights reserved.)