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Tea polyphenol and epigallocatechin gallate (EGCG) were considered as key components of tea. The rapid prediction of these two components can be beneficial for tea quality control and product development for tea producers, breeders and consumers. This study aimed to develop reliable models for tea polyphenols and EGCG content prediction during the breeding process using Fourier Transform-near infrared (FT-NIR) spectroscopy combined with machine learning algorithms. Various spectral preprocessing methods including Savitzky-Golay smoothing (SG), standard normal variate (SNV), vector normalization (VN), multiplicative scatter correction (MSC) and first derivative (FD) were applied to improve the quality of the collected spectra. Partial least squares regression (PLSR) and least squares support vector regression (LS-SVR) were introduced to establish models for tea polyphenol and EGCG content prediction based on different preprocessed spectral data. Variable selection algorithms, including competitive adaptive reweighted sampling (CARS) and random forest (RF), were further utilized to identify key spectral bands to improve the efficiency of the models. The results demonstrate that the optimal model for tea polyphenols calibration was the LS-SVR with R p = 0.975 and RPD = 4.540 based on SG-smoothed full spectra. For EGCG detection, the best model was the LS-SVR with R p = 0.936 and RPD = 2.841 using full original spectra as model inputs. The application of variable selection algorithms further improved the predictive performance of the models. The LS-SVR model for tea polyphenols prediction with R p = 0.978 and RPD = 4.833 used 30 CARS-selected variables, while the LS-SVR model build on 27 RF-selected variables achieved the best predictive ability with R p = 0.944 and RPD = 3.049, respectively, for EGCG prediction. The results demonstrate a potential of FT-NIR spectroscopy combined with machine learning for the rapid screening of genotypes with high tea polyphenol and EGCG content in tea leaves.

Rice is one of the most important food crops in the world, and rice seed varieties are related to the yield and quality of rice. This study used near-infrared (NIR) hyperspectral technology with conventional machine learning methods (support vector machine (SVM), logistic regression (LR), and random forest (RF)) and deep learning methods (LeNet, GoogLeNet, and residual network (ResNet)) to establish variety identification models for five common types of rice seeds. Among the deep learning methods, the classification accuracies of most models were higher than 95%. This study further used the deep learning methods to establish variety identification models for 10 varieties of rice seeds without considering their types. Among them, the ResNet model had the best classification results. The classification accuracy on the test set was 86.08%. This study used the saliency map method to visualize each convolutional neural network (CNN) model to find the band region that contributed the most to the data. The results showed that the bands with the largest data contribution were mainly concentrated at approximately 1300-1400 nm and secondarily concentrated at approximately 1050-1250 nm. The overall results showed that NIR hyperspectral imaging technology combined with deep learning could effectively distinguish rice seeds of different varieties. This method provided an effective way to identify rice seed varieties in a quick and nondestructive manner., Competing Interests: The authors declare no competing financial interest., (© 2022 The Authors. Published by American Chemical Society.)