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Within the capacitive deionization (CDI) realm, the two-dimensional (2D) layered material T 3 C 2 T x MXene has drawn lots of attention because of its excellent electrical conductivity, reversible ionic intercalation/deintercalation capacity, and extensive active sites. However, the performance of MXene is compromised by the fact that its surface is susceptible to oxidation by dissolved oxygen and has inherent defects of self-stacking. In this paper, CuNiFe-Prussian blue analogue@MXene (CuNiFe-PBA@MXene) with three-dimensional (3D) coated heterostructure is successfully prepared by the in-situ coprecipitation. CuNiFe-PBA is uniformly coated on the MXene surface as a functional layer to avoid the re-stacking of MXene, to enlarge their layer spacing, and to slow down the damage of MXene by dissolved oxygen. Based on a coated structure constructed by a good combination of dual pseudocapacitive materials, the CuNiFe-PBA@MXene electrode has excellent electrochemical performance (250F g -1 ). The composed MXene//CuNiFe-PBA@MXene hybrid cell in 500 mg/L NaCl has higher desalination capacity (44.94 mg/g), lower energy consumption (0.66 kWh kg -1 ), and excellent desalination capacity retention (93.46 % after 40 adsorption/desorption cycles). Furthermore, the lower oxidation degree of CuNiFe-PBA@MXene after 40 desalination cycles compared to MXene indicates that the constructed 3D heterogeneous structure can protect MXene and slow down the oxidation of MXene by dissolved oxygen., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier Inc. All rights reserved.)

Purpose: To estimate the time trend in the prevalence of suicidal behaviors including ideation and attempts in Chinese adolescents; to examine the association between suicidal behaviors and overweight (OW)/obesity (OB) and self-perceived weight status., Methods: Data from Youth Risk Behavior Surveys conducted in Ningbo in 2007, 2012, 2017 and 2022 were used for the study. A multistage sampling procedure was used to select respondents aged 12 to 19 years who participated in each survey. Repeated cross-sectional data of anthropometry, weight perception and suicidal behaviors were collected through self-administered questionnaires. A generalized linear model was used to assess associations of ideation and attempts, and weight status and self-perceived weight status., Results: The sample sizes for each survey wave were 937, 889, 1528 and 2655. The prevalence of ideation increased from 11.95% in 2007 to 18.15% in 2022, while the prevalence of attempts remained stable (4.48%-4.26%). An increasing trend in ideation and attempts was found in adolescents who were OW/obese and self-perceived OW/OB from 2007 to 2022. Self-perceived OW/OB adolescents had a significant increase in ideation. Girls had higher likelihood of ideation and attempts for both weight status and self- perceived weight status. While, adolescents aged 12.0-15.9 years had higher odds of ideation than those aged 16.0-19.9 years., Conclusion: Adolescents' self-weight perception, rather than actual weight status, is a critical risk factor of increased likelihood of ideation and attempts in Chinese adolescents. Our findings can support to promote self-esteem growth in school-based health education programmes for prevention of suicidality and psychological health problems., Competing Interests: Declarations. Competing interests: The authors declare no competing interests., (© 2024. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany.)

Diabetes mellitus necessitates strict control of postprandial hyperglycemia via α-glucosidase inhibitors. In this study, novel azole derivatives of Echinocystic acid (EA), a natural pentacyclic triterpenoid, were synthesized through molecular hybridization to enhance hypoglycemic potential. Compound A4 exhibited superior α-glucosidase inhibition (IC 50  = 2.72 μM) than that of EA (IC 50  = 59.91 μM), and acarbose (IC 50  = 342.0 μM). Kinetic analysis revealed mixed-type inhibition (Ki = 15.31 μM, Kis = 10.23 μM), suggesting ternary complex formation. Analysis by spectroscopic studies confirmed A4 altered the enzyme's microenvironment and secondary structure. The combination of molecular docking and molecular dynamics simulations further elucidated the interaction between A4 and α-glucosidase. A4 enhanced binding stability through π-cation and π-π stacking interactions, with low RMSD values indicating structural stability. In vivo studies showed that A4 has an excellent safety profile, with no organ damage observed at a single dose of 0.5 g/kg. In a sucrose loading test in normal mice, A4 demonstrated glucose control comparable to that of acarbose at the same dose. Diabetic mice treated with A4 exhibited reduced fasting blood glucose, improved glucose tolerance, lipid normalization, and antioxidant effects, underscoring its therapeutic promise., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2025 Elsevier Masson SAS. All rights reserved.)

KO t Bu-promoted [3 + 2] cycloaddition of dimethyl sulfoxide (DMSO) with fullerenes has been developed for facile and efficient one-pot synthesis of 1,2,3,4-cyclic sulfoxide-fused [60]/[70]fullerene dihydrides, which offers a versatile platform for the site-selective preparation of various fullerene multiadducts with a wide range of functional groups. The utility of these tetra-functionalized fullerenes is demonstrated by the successful application as electron-transport materials in perovskite solar cells.

With the development of society and technological progress, people pay more and more attention to the inheritance, protection and promotion of cultural heritage. Under the conditions of new technology and new media, communication media and channels are changing rapidly. Improving the speed, breadth and accuracy of the dissemination of information on Guangdong culture in the new era is a new requirement of the times for the dissemination of Guangdong culture. In order to effectively improve the accuracy of Guandong culture recommendation, the study first built a human-machine interactive visual communication platform. Among them, the random forest algorithm was improved by incorporating word embedding technology to facilitate the acquisition of semantic similarity. In addition, it was combined with long-short feature embedding technology and a lightweight gradient boosting tree model, which enabled the acquisition of user interest preferences. The results showed that the model classification accuracy after combining long short-term feature embedding techniques reached 95%, which could better capture the time series features of user behavior. The platform built reduced the risk of overfitting and improved the generalization ability of the model through the integration of multiple decision trees in random forest. Light gradient boosting machine performed excellently and had high computational efficiency when processing large-scale data through an efficient gradient boosting framework. The platform thus built had a recommendation accuracy rate of 97%. At the same time, the platform obtained personalized and similar content recommendation test sections, and the test results were all passed. This proves that the platform can not only accurately recommend Guandong cultural content, but also provide a good communication experience through visualization, providing new ideas and methods for research and practice in the field of Guandong cultural communication., Competing Interests: Declarations. Competing interests: The authors declare no competing interests., (© 2025. The Author(s).)

Arsenic is a ubiquitous hazardous metalloid that poses a significant threat to human health. Although researchers have investigated the detrimental effects of arsenic on the thyroid, a comprehensive exploration of its toxicological impact and underlying molecular mechanisms remains to be conducted. Both this study and our previous reports demonstrated that chronic exposure to sodium arsenite (NaAsO 2 ) results in histological impairment and dysfunction of the thyroid glands in Sprague-Dawley (SD) rats. Proteomic and phosphoproteomic analyses were performed to investigate the molecular mechanisms underlying the effects of chronic NaAsO 2 exposure on thyroid function in SD rats. NaAsO 2 disrupts the synthesis of thyroid hormones (THs) and alters the expression of the THs-synthesizing enzyme dual oxidase 2. In addition, oxidative phosphorylation, the AMP-activated protein kinase signaling pathway, central carbon metabolism in cancer, cysteine and methionine metabolism, cellular response to heat stress, and protein processing in the endoplasmic reticulum were upregulated, whereas glutathione metabolism was downregulated. In conclusion, this study revealed thyroid damage in SD rats induced by chronic NaAsO 2 exposure and elucidated the disrupted molecular pathways, thereby providing novel insights into the molecular mechanisms underlying arsenic exposure and its impact on thyroid function., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier B.V. All rights reserved.)

Background: Distant metastasis is a leading cause of thyroid cancer (TC)-related deaths. Genetic profiling is typically limited to one sample per patient due to cost and sampling-risk concerns. Differences between samples from thyroid and distant metastasis within individual patients are unclear., Methods: Patients with TC and distant metastasis were recruited for genetic analysis., Results: Using a TC-specific NGS panel, 66 specimens from 29 patients were analyzed, identifying 16 mutations and 4 fusions, including two novel fusions (FGFR2-SHTN1 and RFTN1-BRAF). Genetic alterations differed between primary and metastatic sites in nine patients (31%), predominantly in additional oncogenic alterations (89%). More genetic alterations were found at the primary site in three patients and metastatic sites in four. Distinct mutations were found in two patients. A longer time interval between specimen acquisitions was significantly associated with genetic discrepancies (p = 0.032)., Conclusion: Patterns of genetic discrepancies between primary and metastatic TC vary, offering valuable insights for clinical practice., (© 2025 Wiley Periodicals LLC.)

Introduction: TAVO412, a multi-specific antibody targeting epidermal growth factor receptor (EGFR), mesenchymal epithelial transition factor (c-Met), and vascular endothelial growth factor A (VEGF-A), is undergoing clinical development for the treatment of solid tumors. TAVO412 has multiple mechanisms of action for tumor growth inhibition that include shutting down the EGFR, c-Met, and VEGF signaling pathways, having enhanced Fc effector functions, addressing drug resistance that can be mediated by the crosstalk amongst these three targets, as well as inhibiting angiogenesis. TAVO412 demonstrated strong in vivo tumor growth inhibition in 23 cell-line derived xenograft (CDX) models representing diverse cancer types, as well as in 9 patient-derived xenograft (PDX) lung tumor models., Methods: Using preclinical CDX data, we established transcriptomic biomarkers based on gene expression profiles that were correlated with anti-tumor response or distinguished between responders and non-responders. Together with specific driver mutation that associated with efficacy and the targets of TAVO412, a set of 21-gene biomarker was identified to predict the efficacy. A biomarker predictor was formulated based on the Linear Prediction Score (LPS) to estimate the probability of patients or tumor model response to TAVO412 treatment., Results: This efficacy predictor for TAVO412 demonstrated 78% accuracy in the CDX training models. The biomarker model was further validated in the PDX data set and resulted in comparable accuracy., Conclusions: In implementing precision medicine by leveraging preclinical model data, a predictive transcriptomic biomarker empowered by next-generation sequencing was identified that could optimize the selection of patients that may benefit most from TAVO412 treatment., Competing Interests: All authors are employees of Tavotek Biotherapeutics, Inc., with the exception of HZ, who is employed by Crown Bioscience Inc. in Suzhou, PRC., (Copyright © 2025 Jin, Chen, Zhou, Mu, Wu, Zha, Ma, Han and Chiu.)

RNA-binding motif 47 (RBM47) is a recently identified RNA-binding protein involved in early vertebrate development, immune homeostasis, and cancer development. This study examined the biological functions of RBM47 in thyroid-associated ophthalmopathy (TAO). Orbital fibroblasts (OFs) were obtained from the control (n = 6) and TAO groups (n = 6). Protein and gene expression in the obtained samples were investigated using immunohistochemistry, western blotting (WB), and RT-PCR. OFs with RBM47 knockdown were established using small interfering RNA. Subsequently, Oil Red O staining, WB, and RT-PCR were performed to assess adipogenesis in the OFs. The IL-1β-induced expression of proinflammatory molecules and hyaluronan (HA) was determined using enzyme-linked immunosorbent assay and RT-PCR. Moreover, TGF-β-induced fibrosis was evaluated using scratch assays, RT-PCR, and WB. RBM47 expression was markedly increased in orbital tissues and OFs obtained from individuals with TAO. RBM47 knockdown decreased adipogenesis and fibrosis in OFs, and downregulated the levels of insulin-like growth factor 1 receptor (IGF-1R), proinflammatory molecules, and HA. Furthermore, low RBM47 expression downregulated IGF-1R, which subsequently inhibited adipocyte differentiation by decreasing extracellular signal-regulated kinase signalling. These findings indicate that RBM47 may be involved in the regulation of adipogenesis, inflammation, HA production, and fibrosis, highlighting its potential of RBM47 as a therapeutic target for TAO., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 The Authors. Published by Elsevier B.V. All rights reserved.)

Background: The pathogenesis is of livedoid vasculopathy (LV)-a rare, chronic, and recurrent cutaneous vascular obstructive disease-is not fully understood. Conventional anticoagulant therapy sometimes exhibits limited efficacy, and although JAK inhibitors have demonstrated some efficacy in the treatment of refractory LV, the evidence remains insufficient., Case Presentation: This study reports a case of a 14-year-old girl who were failed to maintain remission following treatment with oral rivaroxaban combined with external use of heparin sodium cream. However, the symptoms improved significantly 2 weeks after treatment with baricitinib, and remission was maintained during its gradual reduction., Conclusion: Successful treatment of this refractory patient with baricitinib provides another evidence for the potential therapeutic effect of JAK1/JAK2 inhibitors in LV, and also offers a reference for the dosage tapering regime of baricitinib in LV., Competing Interests: All authors declare that they have no competing interests., (© 2025 Liu et al.)

Asymmetric synthesis of spiro[4H-chromene-3,3'-oxindole] derivatives was realized through an organocatalytic cascade Knoevenagel/Michael/cyclization reaction using a quinidine-derived squaramide. Under the optimized conditions, the reactions of isatins, malononitrile, and sesamol yield the desired spirooxindoles in good yields (75-87%) and moderate to high ee values (up to 90% ee)., Competing Interests: Declarations. Competing interests: The authors declare no competing interests., (© 2024. The Author(s), under exclusive licence to Springer Nature Switzerland AG.)

Intraspinal cold abscesses caused by macrolide-resistant Mycoplasma pneumoniae in patients with X-linked agammaglobulinemia have not yet been described to our knowledge. Here we describe a patient with X-linked agammaglobulinemia who developed an intraspinal cold abscess caused by macrolide-resistant M. pneumoniae . Genetic analysis revealed a hemizygous c.1566 + 1G > C (IVS15 + 1G > C) mutation in BTK gene. The patient showed relatively naive T cells and a significant proliferative defect., Competing Interests: The authors have no funding or conflicts of interest to disclose., (Copyright © 2024 The Author(s). Published by Wolters Kluwer Health, Inc.)

Advanced gastric cancer (AGC) is characterized by poor prognosis and limited responsiveness to immunotherapy. Tumor-associated macrophages (TAMs) play a pivotal role in cancer progression and therapeutic outcomes. In this study, we developed a novel gene signature associated with M1-like TAMs using data from the Gene Expression Omnibus (GEO) and The Cancer Genome Atlas (TCGA) to predict prognosis and immunotherapy response. This gene signature was determined as an independent prognostic indicator for AGC, with high-risk patients exhibiting an immunosuppressive tumor immune microenvironment (TIME) and poorer survival outcomes. Furthermore, Interferon regulatory factor 8 ( IRF8 ) was identified as a key gene and validated through in vitro and in vivo experiments. IRF8 overexpression reshaped the suppressive TIME, leading to an increased presence of M1-like TAMs, IFN-γ + CD8 + T cells, and Granzyme B + CD8 + T cells. Notably, the combination of IRF8 overexpression and anti-PD-1 therapy significantly inhibited tumor growth in syngeneic mouse models. AGC patients with elevated IRF8 expression were found to be more responsive to anti-PD-1 treatment. These findings highlight potential biomarkers for prognostic evaluation and immunotherapy in AGC, offering insights that could guide personalized treatment strategies.

Background/objectives: Optimal nutrition is essential for the health of breastfeeding women and their infants. This study aimed to assess food and nutrient intake and alignment with nutrition guidelines for breastfeeding women living in New Zealand., Methods: Seventy-six breastfeeding women were enrolled in the longitudinal Mother and Infant Nutrition Investigation study and completed a weighed four-day diet diary including supplement use at three months postpartum. The number of servings consumed for each food group were calculated based on the 2020 Eating and Activity Guidelines for New Zealand Adults. Nutrient intakes were compared to the nutrient reference values for Australia and New Zealand., Results: Overall, the percentages of women who met the recommended number of servings for fruits, vegetables, grain foods, meats and milk/milk products were 25%, 0%, 5%, 34%, and 13%, respectively. None of women met the current recommendations for all food groups. Many participants had intakes below the estimated average requirement or adequate intake and were at risk of nutrient inadequacy for vitamin E (55%), vitamin D (53%), manganese (61%), and selenium (55%)., Conclusions: Breastfeeding women had a low alignment with the current dietary guidelines and were at risk of an inadequate intake of vitamin E, D, manganese, and selenium. Research to investigate the barriers and enablers of healthy food choices is needed.

Gastrointestinal perforation in patients with juvenile dermatomyositis has been reported as a life-threatening complication in the literature. However, effective treatment of juvenile dermatomyositis with gastrointestinal perforation remains challenging. We report the case of a patient who developed intestinal perforation 5 months after being diagnosed with anti-nuclear matrix protein 2 antibody-positive juvenile dermatomyositis. We systematically reviewed the literature on the medical and/or surgical treatment of gastrointestinal perforation in juvenile dermatomyositis. In addition to our case, as of October 2023, we identified 29 cases of gastrointestinal perforation in patients with juvenile dermatomyositis. Current treatment options for gastrointestinal perforation in juvenile dermatomyositis mainly include corticosteroids, methylprednisolone pulses, rituximab, intravenous immunoglobulin, cyclophosphamide, methotrexate, cyclosporine A, mycophenolate mofetil, and other traditional disease-modifying anti-rheumatic drugs. Notably, juvenile dermatomyositis complicated by gastrointestinal perforation is always associated with disease severity and activity. However, these are extremely severe patients who may not respond to treatment with methylprednisolone pulses and rituximab. Given the limited efficacy of conventional high-intensity systemic immunosuppressive therapy for juvenile dermatomyositis with gastrointestinal perforation, there is an urgent need to explore novel therapeutic approaches. We report on the successful use of vedolizumab in combination with corticosteroids, cyclophosphamide, and intravenous immunoglobulin as a novel therapeutic strategy for treating juvenile dermatomyositis complicated by gastrointestinal perforation. Importantly, up to now, there has been no report of juvenile dermatomyositis with gastrointestinal perforation treated with vedolizumab combined with traditional disease-modifying anti-rheumatic drugs in children., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (© 2025 Xu, Ye, Jin, Liu, Bao, Huang, Ding, Yang and Jin.)

Streptomyces sp. MJM3502 is a promising producer of rufomycins, which are a class of potent anti-tuberculosis lead compounds. Although the structure, activity, and mechanism of the main rufomycin 4/6 and its analogs have been extensively studied, a significant gap remains in our understanding of the genome sequence and biosynthetic pathway of Streptomyces sp. MJM3502, and its metabolic engineering has not yet been reported. This study established the genetic manipulation platform for the strain. Using CRISPR/Cas9-based technology to in-frame insert the strong kasO∗p promoter upstream of the rufB and rufS genes of the rufomycin BGC, we increased rufomycin 4/6 production by 4.1-fold and 2.8-fold, respectively. Furthermore, designing recombinant strains by inserting the kasO∗p promoter upstream of the biosynthetic genes encoding cytochrome P450 enzymes led to new rufomycin derivatives. These findings provide the basis for enhancing the production of valuable natural compounds in Streptomyces and offer insights into the generation of novel active natural products via synthetic biology and metabolic engineering., Competing Interests: The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: Nguyen-QuangTuan is currently employed by R&D Center, Manbangbio Co. Ltd. Jin-HuaCheng is currently employed by Microbio Healthcare Co. Ltd. Other authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (© 2025 The Authors.)

A series of heterogeneous catalysts, designated as POP-n-Pd (where n = 1, 2, 3, or 4), were synthesized by polymerizing a six-membered N-heterocyclic compound with an alkyl substituted group monomer (S1), using divinylbenzene (DVB) as crosslinkers. This process was followed by the incorporation of palladium (Pd) nanoparticles. The impact of the substituted group and the S1:DVB ratio in the catalysts, together with the reaction conditions, was investigated to assess their influence on the catalytic performance in converting propylamine, carbon dioxide (CO 2 ) and 4-iodoanisole to oxazolidinones. The POP-1-Pd catalyst, featuring a methyl substituted group and a S1:DVB ratio of 1:4, exhibited remarkable efficiency, resulting in an excellent yield of 96 % under room temperature and ambient pressure conditions. Furthermore, it has demonstrated wide applicability across a variety of substrates and in the treatment of lime kiln exhaust gas. Additionally, POP-1-Pd can be used in a gram-scale reaction and maintains its performance after six recycles, with no significant decline in yield. The possible catalytic mechanism is proposed as follows: the catalyst's pores adsorb both CO 2 and substrates, creating a high concentration reactant enrichment microenvironment. This facilitates the activation of both CO 2 and substrates by the imidazole moiety and Pd nanoparticles in the catalyst, thereby generating oxazolidinones., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier Inc. All rights reserved.)

Background: The C-reactive protein-triglyceride glucose index (CTI) is a promising new marker for evaluating the severity of inflammation. Endometriosis (EM) is a prevalent chronic inflammatory condition influenced by estrogen, primarily affecting women of reproductive age. However, no study has demonstrated an association between the CTI and EM., Methods: This cross-sectional study sourced data from females 20-50 years of age from the National Health and Nutrition Examination Survey (NHANES) 1996-2006, and included those with self-reported diagnoses of EM and sufficient information to calculate the CTI, computed as 0.412 × ln (C-reactive protein [CRP]) + ln (triglycerides [mg/dL] × fasting plasma glucose [mg/dL]/2). Multivariate logistic regression, restricted cubic splines, and subgroup analyses were performed to examine the association between the CTI and EM., Results: Data from 2235 women (175 [7.82%] with EM, 2060 [92.18%] without EM [controls]), were included: those with EM exhibited a tendency toward higher CTI (p = 0.005), and CTI was positively associated with the prevalence of EM (p = 0.011). In Model 1, a 1 mg/dL increment in CTI was associated with a 56% higher prevalence of EM (odds ratio [OR] 1.563 [95% confidence interval (CI) 1.295-1.885]; P < 0.001). This association in Model 2 (OR 1.609 [95% CI 1.334-1.941]; p < 0.001) and Model 3 (OR 1.565 [95% CI 1.246-1.966]; p < 0.001) remained significant. Notably, individuals in the uppermost remnant cholesterol tertile exhibited a notably higher prevalence of EM than those in the lowest tertile (OR 3.029, p = 0.051). Restricted cubic splines revealed a nonlinear positive association between CTI and the prevalence of EM. In addition, greater EM prevalence was observed with CTI in those > 40 years of age (OR 1.57 [95% CI 1.16-2.13]), body mass index ≥ 25 kg/m 2 (OR 1.38 [95% CI 1.06-1.80]), smoking ≥ 100 cigarettes (OR 1.43 [95% CI 1.06-1.96]), married or living with partner (OR 1.41 [95% CI 1.09-1.85]), and oral contraceptive use (OR 1.35 [95% CI 1.07-1.69])., Conclusions: CTI was positively associated with EM in women in the United States. Use of the CTI as an indicator of inflammation may provide new insights for the prevention and management of EM., Competing Interests: Declarations. Ethics approval and consent to participate: The portions of this study involving human participants, human materials, or human data were conducted in accordance with the Declaration of Helsinki and were approved by the NCHS Ethics Review Board. The patients/participants provided written informed consent to participate in this study. Consent for publication: Not applicable. Competing interests: The authors declare no competing interests., (© 2025. The Author(s).)

Background: Physical inactivity is a global concern and poses serious health risks. Physical inactivity is exacerbated and affects the health of both generations during the special period of pregnancy. Appropriate physical activity (PA) intervention programmes based on health behaviour change theory are lacking. This study aims to evaluate the effectiveness of a PA intervention based on the multi-theory model (MTM) by assessing changes in PA levels, MTM constructs, exercise self-efficacy, quality of life, prenatal depressive symptoms, gestational weight gain and pregnancy and delivery outcomes., Methods and Analysis: This single-centre, parallel, randomised, controlled trial will be conducted at a tertiary care institution in Hangzhou, China. A total of 72 pregnant women without contraindications to exercise at 14-27 weeks+6 days of gestation will be included. Participants will be randomly assigned to either the intervention or control group. The intervention group will engage in a 12 week PA intervention programme based on the MTM and delivered through WeChat, while the control group will receive standard antenatal care. Data will be collected at baseline, 6 weeks of the intervention, 12 weeks of the intervention and within 1 week of delivery. The primary outcomes will include PA levels measured by accelerometers and the PA in Pregnancy Questionnaire, along with the constructs of the MTM. Secondary outcomes will comprise exercise self-efficacy during pregnancy, quality of life, prenatal depressive symptoms, gestation weight gain and pregnancy and delivery outcomes. Data analysis will adhere to the Consolidated Standards of Reporting Trials 2010 statement and follow the intention-to-treat principle., Ethics and Dissemination: The study followed the ethical guidelines approved by the ethics committee of Women's Hospital School of Medicine Zhejiang University (IRB-20230254-R, Approval Date: 27 July 2023). The results of the study will be submitted for publication and dissemination in a peer-reviewed journal. Participants will receive clarification regarding the study objectives, procedures and will be asked to sign informed consent in written form before the start of the study., Trial Registration Number: ChiCTR2400080843. Prospectively registered on 8 February 2024., Competing Interests: Competing interests: None declared., (© Author(s) (or their employer(s)) 2025. Re-use permitted under CC BY-NC. No commercial re-use. See rights and permissions. Published by BMJ Group.)

Natural products play a pivotal role in drug development, including their direct use as pharmaceuticals and their structural modification, yielding molecules with enhanced therapeutic potential. The discovery of bioactive molecules, lead compounds, and novel drugs is intrinsically linked to the structural optimization of natural products. In this study, forty-one derivatives of dihydroartemisinin (DHA) were synthesized by incorporating fragments with anti-tumour activity via molecular hybridization, and assessed for their anti-proliferative activity against human cancer cell lines (A549, Bel-7402, HCT-116, and SW620) and normal human liver cells (LO2). Most derivatives exhibited superior anti-proliferative activity compared to DHA. Notably, compound A3, featuring a 4-Cl phenyl carbamate moiety, demonstrated significant anti-proliferative activity against HCT-116 cells with an IC 50 of 0.31 μM, making it 16-fold more potent than DHA (IC 50  = 5.10 μM). The anti-proliferative mechanism did not involve cytotoxicity (SI = 54.13), indicating its superior safety profile compared to DHA (SI = 1.65). Further mechanistic studies revealed that compound A3 inhibits HCT-116 cell proliferation by modulating the expression of PI3K/AKT/mTOR and STAT3 proteins. STAT3 downregulation represses the expression of the critical ferroptosis protein glutathione peroxidase 4 (GPX4), aggravating the accumulation of reactive oxygen species (ROS) and depletion of glutathione (GSH). This redox imbalance triggers and accelerates ferroptosis. Additionally, A3 also induces apoptosis by damaging mitochondria and influencing MAPK signaling. Compound A3 arrested cells in the G2/M phase by regulating p53 expression. In an HCT-116 xenograft mouse model, compound A3 exhibited significant anti-cancer efficacy, with a tumor growth inhibition rate of 58.7 %. Therefore, compound A3 thus has the potential to serve as a lead compound for the development of new anti-tumor drugs., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier Masson SAS. All rights reserved.)

Self-healing materials can recover the materials from physical damage, and extend the life of equipment. Metal coordination bonds are supramolecular interactions with tunable stability and sensitivity to external stimuli, which are crucial for developing self-healing materials. Incorporating metal coordination bonds into elastomers and coatings can enable materials to repair damage and enhance material performance. This review details the advance in self-healing elastomers and coatings. The structural characteristics, mechanical properties, and self-healing efficacy of these materials are discussed, and a perspective on the challenges and development directions is given., (© 2025 Wiley-VCH GmbH.)

An efficient stereoselective synthesis of 10-hydroxy-10-(1 H -indol-3-yl)-9-(10 H )-phenanthrene derivatives was realized through an organocatalyzed Friedel-Crafts reaction of phenanthrenequinones and indoles using a ( S , S )-dimethylaminocyclohexyl-squaramide as the catalyst. Under the optimized conditions, the desired chiral products were obtained in good yields (73-90%) with moderate to high ee values (up to 97% ee). Two pairs of synthesized enantiomers were subjected to evaluation of their antiproliferative activities on four types of human cancer cell lines and one human umbilical vein endothelial cell line using the CCK-8 assay. The results indicated that stereoselectivity had obvious impacts on biological activity. ( S )- 4g was found to have optimal cytotoxicity against the A549 cell line and a good safety profile for human normal cells, which was better than the inhibitory activity of the positive control drug (doxorubicin).

The case was a 15-year-old male with a history of paroxysmal supraventricular tachycardia refractory to medical therapy and prior catheter. A repeat electrophysiology study and catheter ablation were applied. Baseline AH and HV intervals were 100 and 55 ms during normal sinus rhythm (NSR), respectively. Programmed atrial stimulation induced a short RP narrow complex tachycardia (HV interval 22 ms) with an incomplete right bundle branch block configuration and right axis deviation. Tachycardia was terminated with ATP 5 mg injection. An atrial premature beat within the His refractory period advanced and reset tachycardia. Entrainment performed from the coronary sinus and left ventricle both showed a post-pacing interval minus tachycardia cycle length (TCL) of 90 ms. After confirming the diagnosis, left atrial mapping along mitral annulus was performed using trans-septal access and accessory pathway potentials were recorded during NSR and tachycardia at the superior mitral annulus. An irrigated ablation catheter guided by 3-D mapping was used to perform ablation during tachycardia. Tachycardia terminated immediately during the first RF application with ensuing automaticity exhibiting a warm-up during radiofrequency delivery and a cooling down upon the suspension of ablation. Therefore, all phenomena of Mahaim-fiber associated tachycardia were observed. In this case, we describe an antidromic atrio-ventricular reentry tachycardia using a left atrio-fascicular fiber inserting into the proximal left anterior fascicle., (© 2024 Wiley Periodicals LLC.)

Implant-associated Staphylococcus aureus (S. aureus) osteomyelitis (IASO) leads to high orthopedic implant failure rates due to the formation of Staphylococcal abscess community within the bone marrow and bacterial colonization in the osteocyte lacuno-canalicular network (OLCN). To address this, antimicrobial peptides (HHC36)-loaded titania nanotubes (NTs) are developed on titanium screws (Ti-NTs-P-A), which integrate pH-responsive polymethacrylic acid to control HHC36 release for eradicating bacteria in IASO. Colony-forming unit assay confirmed that Ti-NTs-P-A screws maintained sustainable antibacterial effectiveness, killing over 65% of S. aureus even after multiple bacterial solution replacements. Notably, Ti-NTs-P-A screws exhibit significant pH-responsive HHC36 release behavior and bactericidal activity, consistent with the phenotype of peptides-killed bacteria from scanning electron microscopy. Transcriptome sequencing results reveal that Ti-NTs-P-A screws interfered with ribosome formation and disrupted the arginine biosynthesis, which is crucial for bacterial survival in acidic environments. In the non-infected implant model, the bone-implant contact ratio of the Ti-NTs-P-A screw is 2.3 times that of the clinically used titanium screw. In an IASO model, Ti-NTs-P-A screws effectively eradicated bacteria within the OLCN, achieving an 80% infection control rate and desirable osteointegration. Collectively, Ti-NTs-P-A screws with pH-responsive antibacterial properties exhibit great potential for eradicating bacteria and achieving osseointegration in IASO., (© 2024 The Author(s). Advanced Healthcare Materials published by Wiley‐VCH GmbH.)

This study aimed to investigate the effects of curcumin-carbon dot conjugates (CUR-CD) on periodontitis. Porphyromonas gingivalis lipopolysaccharide (LPS) was used to establish periodontitis mode in vivo and in vitro. Histological analysis was conducted using hematoxylin and eosin (HE) staining. Bone morphogenetic protein 2 (BMP2) and secreted phosphoprotein 1 (OPN) expression was determined using immunohistochemistry. mRNA levels were detected using reverse transcription quantitative real-time PCR (RT-qPCR). Protein expression was determined using Western blot. N6-methyladenosine (m6A) enrichment was determined using methylated RNA immunoprecipitation (MeRIP) assay. Cytokine release was determined using enzyme-linked immunosorbent assay (ELISA) assay. Osteogenic differentiation was detected using alkaline phosphatase (ALP) staining. The results showed that CUR-CD inhibited the inflammatory response, as well as promoted bone healing in vivo and osteogenic differentiation in vitro. Moreover, CUR-CD downregulated methyltransferase 3 (METTL3), which inhibited m6A modification of endoplasmic reticulum to nucleus signaling 1 (IRElα) and downregulated IRElα expression. However, overexpression of IRElα reversed the effects of CUR-CD, stimulating inflammatory response and inhibiting bone healing and osteogenic differentiation. Collectively, CUR-CD inhibits the progression of periodontitis via downregulating IRElα. Therefore, CUR-CD may be an alternative strategy for periodontitis.

Purpose: To assess the impact of incorporating internet-based resources and popular science workshops on self-care management among hemodialysis patients with arteriovenous fistula., Design: A randomized controlled trial involving 110 hemodialysis patients with arteriovenous fistula, randomly assigned to either the experimental or control group., Methods: The experimental group received self-management training utilizing internet resources and interactive workshops, while the control group received conventional health education methods. After the intervention, the self-management proficiency of both groups was assessed, including comprehension of arteriovenous fistula-related information, satisfaction levels, self-care abilities, engagement in functional exercises, quality of life, and other relevant factors., Findings: Patients in the experimental group exhibited significantly greater proficiency in arteriovenous fistula self-management knowledge, higher levels of patient satisfaction, enhanced self-management abilities, improved functional exercise performance, and better overall quality of life compared to those in the control group ( p  < 0.05)., Conclusions: The combination of internet resources and popular science workshops presents a novel and promising approach for educating and promoting self-care management among hemodialysis patients with arteriovenous fistula. This method holds potential for widespread dissemination and implementation in science education., Clinical Evidence: The study demonstrates the tangible benefits of integrating internet-based resources and popular science workshops into self-management training for arteriovenous fistula among hemodialysis patients, as evidenced by improved knowledge, satisfaction, abilities, exercise performance, and quality of life in the experimental group compared to the control group.

Purpose: This case report aims to present a rare case of endometrial carcinosarcoma, a highly malignant tumor with a poor prognosis. The primary objective is to describe this unique case's clinical presentation, multimodal magnetic resonance imaging (MRI) features, typical histopathological characteristics and surgical treatment., Methods: A detailed analysis of the patient's medical history, preoperative imaging evaluation, and treatment approach was conducted. This case report includes high-resolution images and figures, showcasing MRI scans, surgical treatment, and histopathology slides related to the case., Results: The case report outlines imaging findings of a rare case of endometrial carcinosarcoma. Multimodal imaging such as T1-weighted imaging (T1WI), T2-weighted imaging (T2WI) and multi-b-value diffusion-weighted imaging (DWI), apparent diffusion coefficient (ADC) and dynamic contrast-enhanced (DCE) scanning could accurately identify the histopathological features of the case. Surgical resection is the best treatment, and preoperative imaging evaluation should be particularly important., Conclusion: This case report highlights endometrial carcinosarcoma's rarity and diagnostic challenges. Multimodal MRI has significant value in diagnosing endometrial carcinosarcoma. This technology not only improves the sensitivity, specificity, and accuracy of diagnosis, but also helps to more accurately evaluate the staging and grading of tumors. By comparing imaging features and pathological results, studies have found that multimodal MRI can clearly show the anatomical structure, pathological nature, and extent of the tumor, with a high degree of consistency with the pathological diagnosis. In particular, when differentiating endometrial carcinosarcoma from low-risk endometrial cancer, multimodal MRI combined with serum carbohydrate antigen 125 (CA125) and E-box binding zinc finger protein 1 (ZEB1) detection can further improve the sensitivity and specificity of differential diagnosis. In addition, research has found that the ADC value of the tumor tissue in different pathological grades is related to the multimodal MRI, which helps to better understand the biological behavior and prognosis of the tumor. In summary, multimodal MRI is an effective diagnostic tool that can provide important evidence for the precise diagnosis and treatment of endometrial carcinosarcoma., Competing Interests: Declarations. Conflict of interest: The authors do not have any possible conflicts of interest. Ethical approval: The study did not require ethics approval. Consent to participate: Written informed consent was obtained from the patient. Consent to publication: The authors affirm that human research participants provided informed consent for publication of the images in Fig. 1., (© 2024. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.)

In recent years, it has become a development trend to design multi-application luminescent materials with rare earth ion doping. In this work, a series of Eu 3+ /Sm 3+ doped self-activated Na 2 YMg 2 V 3 O 12 (NYMVO) phosphors were synthesized through a simple high-temperature solid-state reaction method. Interestingly, due to the energy transfer (ET) from the matrix to the activators, the luminescence color of the phosphors changed from turquoise to orange-red and yellow-green under near-ultraviolet (n-UV) 365 nm excitation. Based on the fluorescence intensity ratio of the matrix to Eu 3+ /Sm 3+ , the optical thermometry performances of the NYMVO:0.20Eu 3+ and NYMVO:0.06Sm 3+ phosphors were described. Notably, the maximum absolute sensitivity (S a ) values for NYMVO:0.20Eu 3+ and NYMVO:0.06Sm 3+ phosphors were 0.30 K -1 and 0.032 K -1 , respectively. Correspondingly, the maximum relative sensitivity (S r ) values were 2.17 %K -1 and 1.22 %K -1 , respectively. Moreover, the light-emitting devices based on NYMVO:0.20Eu 3+ and NYMVO:0.06Sm 3+ phosphors had excellent optical properties, with correlated color temperature (CCT) of 4552 K and 4470 K, and color-rendering index (CRI) of 84.6 and 88.5. These results suggested that the two vanadate phosphors prepared had potential applications in both warm white light-emitting diodes (WLEDs) and optical thermometry., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2025. Published by Elsevier B.V.)

Accumulating evidence suggests that the menstrual cycle and its endogenous ovarian hormones, including progesterone (PROG) and estradiol (E2), affect cognitive performance in women, particularly by modulating the prefrontal regions. In this study, we investigated whether differences in PROG and E2 levels modulate attentional control by affecting the prefrontal cognitive control areas. An fMRI scan was conducted on 53 naturally cycling healthy women in their late follicular phase (FP, n = 28) or mid-luteal phase (LP, n = 25) to examine the resting and task states during the completion of a face‒gender Stroop task. PROG was found to be positively correlated with the nodal efficiency of the inferior frontal gyrus (IFG) in the resting-state executive control network. At the behavioral level, while accuracy in categorizing male faces remained similar, participants in the mid-LP were significantly more accurate in categorizing female faces than those in the late FP. At the neural level, both the univariate and multivariate results indicated that higher levels of PROG enhance the detection and resolution of female incongruent faces through the activation of the bilateral IFG. These findings expand evidence of the effects of ovarian hormones on prefrontal-based attentional control in the social context., Competing Interests: Declaration of competing interest The authors have nothing to disclose., (Copyright © 2025 Elsevier Inc. All rights reserved.)

Background: Both rhabdomyosarcoma (RMS) and central retinal artery occlusion (CRAO) are rare medical diseases, and their simultaneous occurrence in the same patient is extraordinarily uncommon. This study presents a comprehensive overview of the clinical manifestations, diagnostic imaging results, and therapeutic interventions of a patient with both conditions., Case Summary: In this report, we present a 30-year-old male who presented with significant protrusion, pain and vision loss and was diagnosed with RMS in the orbit and sinus with CRAO. Following resection of the sinus and orbital mass and enucleation of the right eye, the patient experienced symptom improvement., Conclusion: This article provides an in-depth analysis of the patient's clinical manifestations, the tumor's anatomical origin, and the etiology of CRAO. The concurrent manifestation of both RMS and CRAO is exceedingly uncommon in clinical practice., Competing Interests: Conflict-of-interest statement: All the authors report no relevant conflicts of interest for this article., (©The Author(s) 2024. Published by Baishideng Publishing Group Inc. All rights reserved.)

Asymmetric synthesis of derivatives of spiro[indoline-3,4-pyrrolo[3,4-b]pyridines] were first developed through the organocatalytic cascade of Knoevenagel/Michael/cyclization reactions using a quinidine-derived squaramide. Under the optimized conditions, the three-component reactions of isatins, cyanoacetates, and 3-aminomaleimides yield the desired heterocycle-fused spirooxindoles in good yields (78-91 %) with 53 %-99 % enantiomeric excess (ee). Notably, this reaction enables a broad substrate scope under mild conditions and provides a convenient method for the enantioselective construction of diverse spirooxindoles combined with dihydropyridine and maleimide skeletons, which has great potential for the construction of new bioactive chemical entities., (© 2024 Wiley-VCH GmbH.)

Type VI secretion system 2 (T6SS2) of Vibrio parahaemolyticus is required for cell adhesion and autophagy in macrophages; however, other phenotypes conferred by this T6SS have not been thoroughly investigated. We deleted TssL2, a key component of T6SS2 assembly, to explore the role of the T6SS2 in environmental adaptation and virulence. TssL2 deletion reduced Hcp2 secretion, suggesting that TssL2 played an important role in activity of functional T6SS2. We found that TssL2 was necessary for cell aggregation, wrinkly phenotype formation, and participates in motility and biofilm formation by regulating related genes, suggesting that TssL2 was essential for V. parahaemolyticus to adapt changing environments. In addition, this study demonstrated TssL2 significantly affected adhesion, cytotoxicity, bacterial colonization ability, and mortality in mice, even the levels of the proinflammatory cytokines IL-6 and IL-8, suggesting that TssL2 was involved in bacterial virulence and immunity. Proteome analysis revealed that TssL2 significantly affected the expression of 163 proteins related to ABC transporter systems, flagellar assembly, biofilm formation, and multiple microbial metabolism pathways, some of which supported the effect of TssL2 on the different phenotypes of V. parahaemolyticus. Among them, the decreased expression of the T3SS1 and T2SS proteins was confirmed by the results of gene transcription, which may be the main reason for the decrease in cytotoxicity. Altogether, these findings further our understanding of T6SS2 components on environmental adaption and virulence during bacterial infection. KEY POINTS: • The role of T6SS2 in V. parahaemolyticus was far from clear. • TssL2 participates in cell aggregation, wrinkly phenotype formation, motility, and biofilm formation. • TssL2 is essential for cell bacterial colonization, cytotoxicity, virulence, and proinflammatory cytokine production., Competing Interests: Declarations. Ethics approval: All applicable international, national, and institutional animal care and use guidelines were followed. The animal experiments were approved by the Animal Ethics Committee of the Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences (no. SYXK < HU > 2020–0027). Conflict of interest: The authors declare no conflict of interest., (© 2024. The Author(s).)

Objectives: To investigate the factors influencing the levels of fear regarding psychotherapy in adolescents with depression., Methods: A prospective study was conducted among 258 adolescents with depression who were treated in the outpatient service and inpatient department of the First Affiliated Hospital of Nanchang University from September 2023 to March 2024. A questionnaire survey was performed. The questionnaires used included General Information Questionnaire, Patient Health Questionnaire-9, Generalized Anxiety Disorder-7, Self-Compassion Scale, Self-Concealment Scale, Depression Self-Stigma Scale, and Thoughts About Psychotherapy Survey. The factors influencing the levels of fear regarding psychotherapy were analyzed among the adolescents with depression., Results: The levels of fear regarding psychotherapy in the adolescents with depression were positively associated with the scores on the Patient Health Questionnaire-9, Self-Concealment Scale, and Depression Self-Stigma Scale ( P <0.05), and were negatively associated with the score on the Self-Compassion Scale ( P <0.05). Concerns about therapist responsiveness were negatively associated with the score on the Self-Compassion Scale ( P <0.05), and were positively associated with the scores on the Generalized Anxiety Disorder-7 and Depression Self-Stigma Scale ( P <0.05). The adolescents from one-child families had significantly higher levels of concerns about therapist responsiveness than those from non-one-child families ( P <0.05). The levels of image concerns were positively associated with the scores on Patient Health Questionnaire-9, Self-Concealment Scale, and Depression Self-Stigma Scale ( P <0.05); the levels of coercion concerns were positively associated with the scores of the Patient Health Questionnaire-9 and Self-Concealment Scale ( P <0.05) and were negatively associated with the score on the Self-Compassion Scale ( P <0.05). Middle school students had a significantly higher risk of coercion concerns than university students ( P <0.05)., Conclusions: In adolescents with depression, the levels of fear regarding psychotherapy are associated with educational levels, only-child status, depression symptoms, anxiety symptoms, self-compassion, self-concealment, and depression self-stigma.

Introduction: Thymic stromal lymphopoietin (TSLP) is a master regulator of allergic inflammation against pathogens at barrier surfaces of the lung, skin, and gut. However, aberrant TSLP activity is implicated in various allergic, chronic inflammation and autoimmune diseases and cancers. Biologics drugs neutralizing excess TSLP activity represented by tezepelumab have been approved for severe asthma and are being evaluated for the treatments of other TSLP-mediated diseases., Methods and Results: In this study, we discovered and characterized a novel humanized anti-TSLP antibody TAVO101 with high binding affinity to human TSLP, which blocks TSLP binding to its receptor complexes on cell surface. TAVO101 showed potent neutralization of TSLP activities in the TSLP-driven STAT5 reporter assay and cell proliferation assay. Results from ex vivo studies showed that TAVO101 neutralized TSLP-mediated CCL17 release from primary human CD1c + dendritic cells and proliferation of activated CD4 + T cells. In addition, TAVO101 showed strong efficacy in both TSLP/OVA-induced asthma and imiquimod induced psoriasis models in hTSLP/hTSLPR double knock-in mice. We further conducted Fc engineering to optimize TAVO101 antibody with reduced affinity to Fcγ receptors and C1q protein but with increased affinity to FcRn receptor for half-life extension., Discussion: By recognizing a different epitope, similarly potent neutralization of TSLP activities, and longer circulating half-life than tezepelumab, novel anti-TSLP antibody TAVO101 offers a potential best-in class therapeutics for various TSLP-mediated diseases., Competing Interests: All the authors are employees of Tavotek Biotherapeutics, Inc., (Copyright © 2024 Shi, Yu, Jin, Chen, Mu, Tam, Cho, Tornetta, Han, Fung, Chiu and Zhang.)

Nasopharyngeal carcinoma (NPC) is a malignant tumor with a high incidence rate in certain regions. MicroRNA (miRNA/miR)-22-3p is implicated in the regulation of tumorigenesis and progression. However, the biological role of miRNA-22-3p in the progression of NPC remains unclear. The present study aimed to assess the effects of miRNA-22-3p overexpression on the cell viability and migration of NPC cells. The cell viability and migration of HK-1 cells was evaluated using Transwell, wound healing and Cell Counting Kit-8 assays. To assess the epithelial-mesenchymal transition ability of NPC cells, the expression of E-cadherin, vimentin and N-cadherin was evaluated using western blot analysis. The results revealed expression of miRNA-22-3p was significantly decreased in NPC tissues compared with para-cancerous tissues. Decreased expression of miRNA-22-3p was also observed in NPC cell lines (C666-1 and HK-1). The overexpression of miRNA-22-3p reduced HK-1 cell viability and migration. In addition, a dual luciferase reporter assay revealed that miRNA-22-3p functioned as a molecular sponge for forkhead box protein 1 (FOXP1). Notably, FOXP1 overexpression counteracted the suppressive effects induced by transfection with miRNA-22-3p mimic on HK-1 cell viability and migration. Therefore, these data indicate that miRNA-22-3p may be a clinically valuable biomarker for the therapy of NPC., Competing Interests: The authors declare that they have no competing interests., (Copyright: © 2024 Jin et al.)

Background: Genetic factors play an important role in neonatal hyperbilirubinemia (NH) caused by genetic diseases., Aim: To explore the characteristics of genetic mutations associated with NH and analyze the correlation with genetic diseases., Methods: This was a retrospective cohort study. One hundred and five newborn patients diagnosed with NH caused by genetic diseases were enrolled in this study between September 2020 and June 2023 at the Second Affiliated Hospital of Xiamen Medical College. A 24-gene panel was used for gene sequencing to analyze gene mutations in patients. The data were analyzed via Statistical Package for the Social Sciences 20.0 software., Results: Seventeen frequently mutated genes were found in the 105 patients. Uridine 5'-diphospho-glucuronosyltransferase 1A1 ( UGT1A1 ) variants were identified among the 68 cases of neonatal Gilbert syndrome. In patients with sodium taurocholate cotransporting polypeptide deficiency, the primary mutation identified was Na+/taurocholate cotransporting polypeptide Ntcp ( SLC10A1 ). Adenosine triphosphatase 7B ( ATP7B ) mutations primarily occur in patients with hepatolenticular degeneration (Wilson's disease). In addition, we found that UGT1A1 and glucose-6-phosphate dehydrogenase mutations were more common in the high-risk group than in the low-risk group, whereas mutations in SLC10A1 , ATP7B , and heterozygous 851del4 mutation were more common in the low-risk group., Conclusion: Genetic mutations are associated with NH and significantly increase the risk of disease in affected newborns., Competing Interests: Conflict-of-interest statement: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (©The Author(s) 2024. Published by Baishideng Publishing Group Inc. All rights reserved.)

The effects of Helicobacter pylori (Hp)-related chronic gastritis on gastrointestinal microorganisms or brain neurotransmitters are not fully understood. Here, this study selected SPF C57BL/6 mice to set up a Hp-related chronic gastritis experiment group and a blank control group, and used omics to explore the specific effects of Hp-related chronic gastritis on gastrointestinal microorganisms and brain neurotransmitters in mice. The Tyramine (TyrA) content in the female experiment group's brain was considerably reduced compared to the female control group ( p < 0.01), and TyrA was strongly correlated with 13 gastrointestinal microorganisms with significant differences, such as Acinetobacter&#95;baumannii ( p < 0.05). The His content in the male experiment group's brain was significantly higher than that in the male control group ( p < 0.05), and His was strongly correlated with four gastrointestinal microorganisms with significant differences, such as Acinetobacter&#95;baumannii ( p < 0.05). The Levodopa (DOPA) content in the female control group's brain was significantly lower than that in the male control group ( p < 0.05), and DOPA was strongly correlated with 19 gastrointestinal microorganisms with significant differences, such as Achromobacter&#95;xylosoxidans ( p < 0.05). The contents of L-Glutamine (Gln), L-Glutamine (GABA), Noradrenaline hydrochloride (NE), and Adrenaline hydrochloride (E) in the female experiment group's brain were significantly lower than those in the male experiment group ( p < 0.05), and Gln, GABA, NE, and E were strongly correlated with 41, 28, 40, and 33 gastrointestinal microorganisms with significant differences ( p < 0.05), respectively. These results indicate that Hp-related chronic gastritis could affect gastrointestinal microorganisms and brain neurotransmitters in mice with certain gender differences, and the changes in brain neurotransmitters might be related to the changes in gastrointestinal microorganisms., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2024 Liu, Lin, Zhang, Zhang, Fang and Hu.)

Arsenic, a well-known environmental endocrine disruptor, exerts interference on the body's endocrine system. Our previous investigations have demonstrated that chronic exposure to sodium arsenite (NaAsO 2 ) can induce thyroid damage and dysfunction in Sprague-Dawley (SD) rats. Vitamin D (VD) is an indispensable fat-soluble vitamin that plays a crucial role in maintaining thyroid health. In recent years, numerous studies have demonstrated the association between VD deficiency and the development of various thyroid disorders. However, the precise intervention roles and mechanisms of VD in arsenic-induced thyroid injury remain elusive. This study aimed to investigate the intervention effect of VD on NaAsO 2 -induced thyroid dysfunction in SD rats. The results demonstrated that exposure to NaAsO 2 activates the TLR4/NF-κB signaling pathway in thyroid tissue of rats, leading to apoptosis of thyroid cells and subsequent inflammatory damage and disruption of serum thyroid hormone secretion. Supplementation with TAK-242 (a TLR4 inhibitor) and VD effectively inhibits the activation of the TLR4/NF-κB signaling pathway in rat thyroid tissue exposed to NaAsO 2 , thereby reducing the inflammatory damage and dysfunction caused by arsenic exposure. In conclusion, the findings of this study offer innovative insights into the application of VD in the prevention and treatment of thyroid dysfunction caused by arsenic exposure.

Objective: Bipolar affective disorder (BAD) is a mood disorder with high morbidity and mortality. Quetiapine can be used in the treatment of patients with BAD; however, the precise administration regimen of quetiapine in these patients is still unknown. In this study, a population pharmacokinetic (PPK) model of quetiapine in patients with BAD was constructed based on model-informed precision dosing (MIPD) and real-world clinical data and an optimal initial dose of quetiapine in these patients was recommended., Methods: A total of 99 patients with BAD treated with quetiapine were included. At the same time, the quetiapine concentrations, the physical and chemical indices of the patients, and the drug combination information were collected. A quetiapine PPK model for patients with BAD was then constructed and an initial dose based on Monte Carlo simulation was recommended., Results: In the final model of quetiapine for patients with BAD, the apparent oral clearance (CL/F) and the apparent volume of distribution (V/F) were 76.1 L/h and 530 L, respectively. For patients with BAD weighing 40-66 kg, the initial dose recommendation was 16 mg kg -1 day -1 , the probability of reaching the therapeutic window was 78.8%-82.2%, and the probability of exceeding the upper limit of the therapeutic window was 5.2%-10.3%. For patients with BAD weighing 66-120 kg, the initial dose recommendation was 12 mg kg -1 day -1 , the probability of reaching the therapeutic window was 81.5%-85.5%, and the probability of exceeding the upper limit of the therapeutic window was 3.6%-8.1%., Conclusion: The present study, for the first time, recommended an initial dose of quetiapine in patients with BAD based on MIPD and real-world data, providing an individualized reference for the administration of quetiapine in these patients., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2024 Zheng, Jin, Yin, Chen, He, Liu, Zhang and Wang.)

Objective: Rapidly progressive interstitial lung disease (RP-ILD) is a frequent and serious manifestation of idiopathic inflammatory myopathy (IIM) associated with poor outcomes. Plasma exchange (PE) can quickly remove pathogenic substances from the blood. Therefore, PE may be efficacious in IIM patients who have elevated levels of autoantibodies, cytokines and chemokines, fighting for time for immunosuppressive therapy. However, the value of adding PE to immunosuppressants remains unclear. The purpose of this study was to determine the short-term outcomes, including the survival rate at 6 months and change of the laboratory data, of PE in combination with immunosuppressants and/or biologics in the treatment of IIM-RP-ILD., Methods: We searched PubMed, Embase and Cochrane Library to find reports of interest published from inception to March 4, 2024. STATA 15.1 was used for data analysis. A fixed or random-effects model with inverse-variance weighting was used to estimate the pooled risk ratio (RR) and 95% confidence interval (CI)., Results: Two hundred and thirty studies were identified. Eleven studies, including five retrospective cohort studies, four case-control studies and two case series, were included. PE was performed on 114 patients. The survival rate at 6 months was 80% (95%CI = 64%-92%), with moderate heterogeneity ( I 2 =63.45%, p  < 0.05). Moreover, the 6-month survival rate was significantly better in the PE group than in the non-PE group (RR, 1.34; 95% CI = 1.05-1.71, I 2 =30.7%; p  = 0.194). ILD-related serum markers, including ferritin, KL-6 and anti-MDA-5 antibody titres, were significantly suppressed by a series of PE treatments ( p  < 0.05)., Conclusion: The application of PE therapy plus treatment with corticosteroids, immunosuppressants and/or biologics was effective for patients with IIM-RP-ILD. PE may have additional supportive effect in intractable disease.

Rationale and Objectives: The objective of this study was to measure the safety and efficacy of thermal ablation, including radiofrequency ablation (RFA) and microwave ablation (MWA), for patients with stage I non-small cell lung cancer (NSCLC)., Materials and Methods: The databases PubMed was searched from inception to November 2023 to identify relevant studies. Statistical analyses were performed with R version 3. 6. 3., Results: Thirty-three studies involving 1400 patients were finally included. According to our study, the incidence of patients with stage I NSCLC who were older than 60 years old was 98 % (95 % CI [94-100 %]); the lesions were mostly located in RUL (Right Upper Lobe) and LUL (Left Upper Lobe), and the incidence of the two sites was 29 % (95 % CI [23-35 %]) and 27 % (95 % CI [21-33 %]), respectively; the types of lung cancers mainly included adenocarcinoma, squamous carcinoma, and large-cell lung cancer, of which adenocarcinoma accounted for the largest proportion of 63 % (95 % CI [56-70 %]); the causes of death were mainly categorized into cancer-related (57 %, 95 %CI[40-74 %]) and noncancer-related (40 %, 95 %CI [23-58 %]); the common complications in the postoperative period were pneumothorax and pain, with the incidence of 33 % (95 %CI[24-44 %]) and 33 % (95 %CI[19-50 %]), and the rate of the postoperative complications in MWA was slightly higher than those in RFA; the local recurrence rate was 23 % (95 %CI[17-29 %]) and the distant recurrence rate was 18 % (95 %CI[7-32 %]); the pooling result showed the rate of 1-, 2-, 3-, and 5-year survival rate were 96 %, 81 %, 68 %, and 42 %, the Cancer-specific survival (CSS) rates at 1, 2, 3, and 5 years were 98 %, 88 %, 75 %, and 58 %, Disease-free survival (DFS) rates at 1, 2, 3, and 5 years were 87 %, 63 %, 57 %, and 42 %, there were no significant differences existed between the RFA group and MWA group in survival rate, CSS and DFS., Conclusion: Ablation therapy is safe and effective for stage I NSCLC patient. MWA and RFA have comparable efficacy, safety, and prognosis, which could be recommended for patients with stageⅠNSCLC, especially for patients who cannot tolerate open surgery., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 The Association of University Radiologists. Published by Elsevier Inc. All rights reserved.)

Objective: Participating in physical activity during pregnancy has benefited a lot from maternal and child health. However, there are few longitudinal studies describing activity patterns and related factors during pregnancy. The aim of this study is to investigate longitudinal physical activity changes and the influencing factors of Chinese pregnant women., Methods: From January to August 2020, 240 pregnant women were recruited in Hangzhou, China. Physical activity during pregnancy was assessed in the first, second, and third trimesters of pregnancy by using the Pregnancy Physical Activity Questionnaire., Results: The daily energy consumption during first, second, and third trimesters was 20.55, 20.76, 17.19 METs-h/d. The results of repeated-measure analysis of variance and pairwise comparison showed that the total daily energy consumption of physical activity in the third trimester was significantly lower than that in the first and second trimesters, with statistical significance ( p  < 0.001). The generalized estimation equation showed that education level, pre-pregnancy BMI, gravidity, unnaturally conceived and pre-pregnancy exercise habits were the influencing factors of physical activity during pregnancy ( p  < 0.05)., Conclusion: Physical activity levels of pregnant women during different trimester were not optimistic. In order to improve physical activity during pregnancy and promote the health status of both mother and the developing baby, more attention should be paid on pregnant women with low education level, high BMI before pregnancy, primipara, unnaturally conceived and no good exercise habits before pregnancy.

Objective: Evaluation of the accuracy of the wrist-type fully automatic blood pressure (BP) monitor: DBP-2242 in adolescent and adult populations according to ISO 81060-2 : 2018+Amd.1 : 2020., Methods: BP measurements were taken from the subjects using the same-arm sequential method, and BP data measured by a mercury sphygmomanometer was used as the standard., Results: This study analyzed 262 sets of data from 89 subjects. According to criterion 1, the mean difference between the SBP of the test and reference devices was -0.57 ± 7.31 mmHg, and the DBP was -2.27 ± 7.17 mmHg, which is in accordance with the requirements. According to criterion 2, the average difference between the SBP was -0.57 ± 6.25 mmHg and the DBP was -2.27 ± 5.99 mmHg, which is in accordance with the requirements., Conclusion: Wrist-type fully automatic BP monitor: DBP-2242 complies with ISO 81060-2 : 2018+Amd.1 : 2020 and can be used for BP measurement in adolescent and adult populations., (Copyright © 2024 The Author(s). Published by Wolters Kluwer Health, Inc.)

Background: Patients with primary aldosteronism (PA) exhibit a high prevalence of diabetes mellitus (DM). However, the relationship between visceral adipose tissue (VAT) and new-onset diabetes mellitus (NODM) in PA patients remains unclear., Objectives: To explore the association between VAT and the risk of NODM in PA patients., Design: This is a prospective cohort study spanning 10 years (2010-2020)., Methods: A total of 342 PA patients were enrolled prospectively. Abdominal adiposity indexes, including VAT area, VAT ratio, subcutaneous adipose tissue (SAT) area, and SAT ratio, were measured using a computed tomography-based software at diagnosis., Results: Of 342 PA patients (46.2% male, mean age 50.8 ± 11.2 years), 35 (10.2%) developed NODM over a mean follow-up of 7.4 years. A positive nonlinear association between NODM risk and Log (VAT ratio) ⩾ -0.72 was observed (high-VAT group). High VAT (odds ratio (OR), 6.09; p  = 0.005), older age (OR, 1.09; p  < 0.001), higher body mass index (OR, 1.24; p  < 0.001), higher waist-to-hip ratio (OR, 1.11, p  < 0.001), lower baseline aldosterone (OR, 0.99, p  = 0.011), higher diastolic blood pressure (OR, 1.05, p  = 0.012), and lower systolic blood pressure (OR, 0.98, p  = 0.045) as risk factors for high VAT. Adrenalectomy did not significantly associate with reduced NODM risk (OR, 0.49; p  = 0.292)., Conclusion: Our findings highlight that 10.2% of PA patients develop NODM over a mean follow-up of 7.4 years, with high VAT increasing the risk. Baseline VAT is a key determinant of NODM development in PA patients, regardless of targeted treatments., (© The Author(s), 2024.)

Objective: Vaginal natural orifice transluminal endoscopic surgery (vNOTES) is widely recognized for its advantages, including reduced postoperative pain and the absence of visible scarring. However, the anatomical specificity required for vNOTES may increase the risk of injury to nearby organs, such as the rectum and bladder. This study aims to evaluate the safety and potential benefits of vNOTES compared to transumbilical laparoendoscopic single-site surgery (LESS) for Hysterectomy., Methods: The Longitudinal Vaginal Natural Orifice Transluminal Endoscopic Surgery Study (LovNOTESS), conducted in Chengdu, China, included 192 patients who underwent hysterectomy between May and October 2023. This study prospectively collected and compared perioperative and follow-up data between the two groups., Results: The vNOTES group demonstrated shorter surgery times, postoperative flatus time, and hospital stays, but it also had a higher conversion rate. Specifically, the vNOTES approach reduced surgery duration by approximately 29.8 min (95% CI: -41.31, -18.34, P < 0.001) but increased intraoperative blood loss by about 41.82 mL (95% CI: 25.81, 57.82, P < 0.001)., Conclusion: By combining laparoscopy with traditional vaginal surgery, vNOTES offers advantages such as shorter surgery times, faster postoperative recovery, reduced hospital stays, greater minimal invasiveness, and improved cosmetic outcomes. However, it is essential for surgeons to continually enhance and standardize preoperative assessments and surgical techniques to minimize conversion rates and reduce intraoperative blood loss., Trial Registry Number: ChiCTR2200059282, China Clinical Trials Registry, April 28, 2022., Competing Interests: Declarations. Ethics approval and consent to participate: This study is based on a Longitudinal Vaginal Natural Orifice Transluminal Endoscopic Surgery Study (LovNOTESS) conducted in Chengdu (China Clinical Trials Registry ChiCTR2200059282, April 28, 2022) and approved by the Ethics Committee of Chengdu Women and Children’s Central Hospital (No. 202130). This study was conducted strictly in accordance with the Declaration of Helsinki. Before the surgery, all patients were well-informed of the potential risks and advantages of vNOTES and other alternative surgical treatments, including TU-LESS or multi-port laparoscopy, and expressed their preference for vNOTES. Written informed consent obtained from all the participants. Consent for publication: Not applicable. Competing interests: The authors declare no competing interests., (© 2024. The Author(s).)

Background: The optimal treatment for anterior cruciate ligament (ACL) injuries with early-stage symptomless osteoarthritis (OA) remains unclear. This study aimed to compare the clinical outcomes of ACL reconstruction (ACLR) in patients with ACL injury in the presence and absence of early-stage symptomless OA., Methods: Medical records of patients with early-stage symptomless OA who sustained ACL injury and underwent ACLR from January 2018 to December 2020 at a single institution were retrospectively reviewed. Radiography and magnetic resonance imaging were performed preoperatively to identify ACL injury and OA. Patients with ACL injury and early-stage symptomless OA (combined group, n = 14) were matched at a 1:2 ratio with patients with ACL injury (isolated group, n = 32). Data on demographic characteristics, pain and functional outcomes (visual analogue scale, Tegner, Lysholm, and International Knee Documentation Committee scores and range of motion), and imaging outcomes were collected. Complications were recorded, and outcomes were compared between the groups., Results: Forty-six patients (26 males, 20 females) were included in this study, among whom 14 had ACL injury and early-stage symptomless OA, whereas 32 sustained isolated ACL injury (average follow-up duration: 3.4 ± 1.0 years). At the final follow-up, pain and functional outcomes similar to those in patients with isolated ACL-deficient knees were achieved in the combined group; acute-stage ACLR resulted in significantly better outcomes (p < 0.05). No significant radiographic progression of OA or complications were observed., Conclusions: Knee function in patients with ACL injuries and early-stage symptomless OA who underwent ACLR was the same as that in patients with isolated ACL-deficient knees. Furthermore, ACLR had a beneficial effect on knee outcomes. The subgroup analysis revealed that acute-stage ACLR could lead to better outcomes in patients with ACL injuries and early-stage symptomless OA., Competing Interests: Declarations. Ethics approval and consent to participate: This study was approved by the Ethics Committee of the Affiliated Hospital of Zunyi Medical University (approval no.: KLL-2023-229). All methods were performed in accordance with the Chinese Ethical Guidelines for Medical and Biological Research Involving Human Subjects. Written informed consent was obtained from each participant. Consent for publication: Not applicable. Competing interests: The authors declare no competing interests., (© 2024. The Author(s).)

Inappropriate CD4 + T helper (T H ) cell differentiation leads to progression of inflammatory and autoimmune diseases, yet the regulatory mechanisms governing stability and activity of transcription factors controlling T H cell differentiation remain elusive. Here, we describe how pseudokinase serine threonine kinase 40 (STK40) facilitates T H 1/T H 17 differentiation under pathological conditions. STK40 in T cells is dispensable for immune homeostasis in resting mice. However, mice with T cell-specific deletion of STK40 exhibit attenuated symptoms of experimental autoimmune encephalomyelitis and colitis, accompanied by diminished T H 1 and T H 17 cell differentiation. Mechanistically, STK40 facilitates K48-linked polyubiquitination and proteasomal degradation of FOXO1/4 through promoting their interaction with E3 ligase COP1. Inhibition of FOXO4 or FOXO1, respectively, restores differentiation potential of STK40-deficient T H 1/T H 17 cells. Together, our data suggest a crucial role of STK40 in T H 1 and T H 17 cell differentiation, thereby enabling better understanding of the molecular regulatory network of CD4 + T cell differentiation and providing effective targets for the treatment of autoimmune diseases.

Objective: Although quetiapine has been approved for use in schizophrenic patients, its individualized dosage regimen remains unclear, especially with respect to drug-drug interactions (DDIs). Thus, we investigated the potential DDIs and optimal initial dosage of quetiapine in schizophrenic patients based on population pharmacokinetics (PPK)., Methods: Ninety-six schizophrenic patients treated with quetiapine were included to establish the PPK model, which also includes coadministration of multiple drugs., Results: It was found that the patient weights and fluvoxamine or duloxetine coadministration affected quetiapine clearance in schizophrenic patients. Without fluvoxamine or duloxetine coadministration, 16 and 12 mg/kg/day of quetiapine were recommended to schizophrenic patients whose weights were in the ranges of 40-50 and 50-120 kg, respectively. With fluvoxamine coadministration, 8 mg/kg/day of quetiapine was recommended to patients with weights in the range of 40-120 kg. With duloxetine coadministration, 8 mg/kg/day of quetiapine was recommended to patients with weights in the 40-120 kg range. With simultaneous coadministration of fluvoxamine and duloxetine, 4 mg/kg/day of quetiapine was recommended to patients with weights in the 40-120 kg range., Conclusion: The present study was a pilot effort at investigating the potential DDIs and optimal initial dosage of quetiapine in schizophrenic patients based on PPK. The initial dosages of quetiapine administered to the patients were optimized according to the coadministration of fluvoxamine or duloxetine., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2024 Chen, Zhang, Yin, Jin, He, Liu, Zhang and Wang.)