1. Immobilization of the Gas Signaling Molecule H2 S by Radioisotopes: Detection, Quantification, and In Vivo Imaging.
- Author
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Sarkar S, Ha YS, Soni N, An GI, Lee W, Kim MH, Huynh PT, Ahn H, Bhatt N, Lee YJ, Kim JY, Park KM, Ishii I, Kang SG, and Yoo J
- Subjects
- Animals, Copper Radioisotopes administration & dosage, Gases analysis, Mice, Optical Imaging, Organometallic Compounds administration & dosage, Positron-Emission Tomography, Rats, Copper Radioisotopes chemistry, Hydrogen Sulfide analysis, Organometallic Compounds chemistry
- Abstract
Hydrogen sulfide (H2 S) has multifunctional roles as a gas signaling molecule in living systems. However, the efficient detection and imaging of H2 S in live animals is very challenging. Herein, we report the first radioisotope-based immobilization technique for the detection, quantification, and in vivo imaging of endogenous H2 S. Macrocyclic (64) Cu complexes that instantly reacted with gaseous H2 S to form insoluble (64) CuS in a highly sensitive and selective manner were prepared. The H2 S concentration in biological samples was measured by a thin-layer radiochromatography method. When (64) Cu-cyclen was injected into mice, an elevated H2 S concentration in the inflamed paw was clearly visualized and quantified by Cerenkov luminescence and positron emission tomography (PET) imaging. PET imaging was also able to pinpoint increased H2 S levels in a millimeter-sized infarcted lesion of the rat heart., (© 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.)
- Published
- 2016
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