1. Detection of infectious laryngotracheitis virus in chickens using a non-radioactive DNA probe.
- Author
-
Keam L, York JJ, Sheppard M, and Fahey KJ
- Subjects
- Animals, Antigens, Viral analysis, Cloning, Molecular, DNA Probes, Enzyme-Linked Immunosorbent Assay, Herpesviridae Infections diagnosis, Herpesviridae Infections microbiology, Herpesvirus 1, Gallid genetics, Herpesvirus 1, Gallid immunology, Nucleic Acid Hybridization, Poultry Diseases diagnosis, Predictive Value of Tests, Specific Pathogen-Free Organisms, Trachea microbiology, Chickens, DNA, Viral analysis, Herpesviridae Infections veterinary, Herpesvirus 1, Gallid isolation & purification, Poultry Diseases microbiology
- Abstract
A DNA hybridization assay using a non-radioactive probe has been developed for the detection of infectious laryngotracheitis virus (ILTV) DNA. A 1.4-kilobase pair BamHI fragment of ILTV genomic DNA was cloned and then labeled by one of two methods; nick translation using 32P-dATP or non-radioactive labeling using a commercially available DNA labeling and detection kit. The non-radioactive DNA labeling method proved to be as sensitive as the radioactive method. Using the non-radioactive probe, ILTV DNA was readily detected in tracheal samples from acutely infected chickens and also from convalescent chickens at a time when viral antigen could no longer be detected by the enzyme-linked immunosorbent assay or the virus could no longer be reisolated. This technique provides a safe and effective means of identifying field outbreaks of ILTV and also may detect latent ILTV infections relatively quickly and inexpensively.
- Published
- 1991