1. Prostaglandin E 2 suppresses KCNH1 gene expression and inhibits the proliferation of CaSki cervical cells through its four prostanoid PTGER subtypes.
- Author
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Cortes-Hernández U, Lizardi-Aguilera TM, Noriega-Mejía BJ, González-Macías J, García-Quiroz J, Díaz L, Larrea F, and Avila E
- Subjects
- Humans, Female, Cell Line, Tumor, Ether-A-Go-Go Potassium Channels genetics, Ether-A-Go-Go Potassium Channels metabolism, Human papillomavirus 16 genetics, Human papillomavirus 16 metabolism, Human papillomavirus 16 pathogenicity, HeLa Cells, Gene Expression Regulation, Neoplastic, Receptors, Prostaglandin E metabolism, Receptors, Prostaglandin E genetics, Dinoprostone metabolism, Cell Proliferation drug effects, Uterine Cervical Neoplasms genetics, Uterine Cervical Neoplasms metabolism, Uterine Cervical Neoplasms virology, Uterine Cervical Neoplasms pathology
- Abstract
The main risk factor for cervical cancer is the persistent infection of high-risk HPV subtypes, notably HPV16. Another contributing factor is proinflammatory prostaglandin E
2 (PGE2 ), a lipid abundantly found in seminal fluid. PGE2 , along with its receptors (PTGER1-4), contributes to cancer development; however, its specific role in the proliferation of cervical cancer models with high HPV16 copy numbers remains unclear. In this study, we investigated the effects of PGE2 on the proliferation of CaSki cells, a cell line with a high HPV16 viral load. Surprisingly, PGE2 inhibited CaSki cell proliferation, while it increased the proliferation of SiHa, HeLa, and C-33 A cervical cancer cells. The effect of PGE2 on CaSki cell proliferation was specific, as estradiol increased cell growth. Furthermore, PGE2 suppressed expression and promoter activity of the cervical tumoral marker KCNH1. To discern the specific role of each receptor in cell proliferation, we generated stable CaSki cell lines overexpressing each receptor alongside control cells with an empty vector. Notably, PGE2 significantly inhibited cell proliferation in all stable transfected CaSki cells, suppressing oncogenic KCNH1 expression and its promoter activity. In conclusion, our findings indicate that PGE2 inhibits the proliferation of CaSki cervical cancer cells with a high HPV16 load, at least in part, by suppressing the expression of the oncogenic KCNH1 gene., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier B.V. All rights reserved.)- Published
- 2025
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