1. A highly sensitive ratiometric fluorescence probe for sensing and imaging sulfite in food samples and living cells.
- Author
-
Yao P, Lei Z, Liu C, Bian Y, Wu J, He S, and Zeng X
- Subjects
- Humans, HeLa Cells, Limit of Detection, Food Analysis methods, Optical Imaging methods, Sulfites analysis, Fluorescent Dyes chemistry, Fluorescent Dyes chemical synthesis, Spectrometry, Fluorescence methods
- Abstract
In this work, a ratiometric and chromogenic fluorescent probe 1 was synthesized for the detection of SO
3 2- . The probe 1 at PBS (10 mM, pH = 7.4) presented a marked emission band at 661 nm. Upon addition of SO3 2- ions, a highly emissive adduct with a marked fluorescence at 471 nm were obtained through a Michael addition. The probe 1 displayed a noticeable fluorescence ratiometric response with a large shift (190 nm) in emission wavelength. The probe can quantitatively detect SO3 2- with high specificity, fast response (within 130 s) as well as low detection limit (13 nM), and a large Stokes shift (139 nm). Fluorescence imaging of HeLa cells indicated that 1 could be used for monitoring the intrinsically generated intracellular SO3 2- in living cells by ratiometric fluorescence imaging. Furthermore, 1 could be application in real water and sugar samples with high sensitivity and good recoveries., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier B.V. All rights reserved.)- Published
- 2024
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