1. Vertical distribution of ammonia-oxidizing archaea and bacteria in sediments of a eutrophic lake.
- Author
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Zhao D, Zeng J, Wan W, Liang H, Huang R, and Wu QL
- Subjects
- DNA, Archaeal chemistry, DNA, Archaeal genetics, DNA, Bacterial chemistry, DNA, Bacterial genetics, Molecular Sequence Data, Oxidation-Reduction, Oxidoreductases genetics, Real-Time Polymerase Chain Reaction, Sequence Analysis, DNA, Ammonia metabolism, Archaea classification, Archaea isolation & purification, Bacteria classification, Bacteria isolation & purification, Biota, Lakes microbiology
- Abstract
In order to characterize the vertical variation of abundance and community composition of ammonia-oxidizing archaea (AOA) and ammonia-oxidizing bacteria (AOB) in sediments of a eutrophic lake, Lake Taihu, molecular techniques including real-time PCR, clone library, and sequencing were carried out in this study. Abundances of archaeal amoA gene (ranged from 2.34 × 10(6) to 4.43 × 10(7) copies [g dry sediment](-1)) were higher than those of bacterial amoA gene (ranged from 5.02 × 10(4) to 6.91 × 10(6) copies [g dry sediment](-1)) for all samples and both of them exhibited negative correlations with the increased depths. Diversities of archaeal and bacterial amoA gene increased with the elevated depths. There were no significant variations of AOB community structures derived from different sediment depths, whereas obvious differences were observed for the AOA community compositions. The information acquired in this study would be useful to elucidate the roles of AOA and AOB in the nitrogen cycling of freshwater ecosystems.
- Published
- 2013
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