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As genomics initiatives have spread around the world-often in the name of genetic diversity and inclusion-they have not only invoked promises of a medical revolution, but also revived categories of human difference that resemble erstwhile racial classifications. This is despite the fact that geneticists broadly dismissed racial categories as obsolete and unfounded after the Human Genome Project was completed in 2003. In fact, contemporary genomics initiatives have often ended up reinforcing ethnocentric and nativist conceptions of difference, drawing intense criticism from activists and critical social scientists. This roundtable brings leading population geneticists grappling with the question of genetic identity and ancestry, especially in the global South, together with some of the most prominent scholars of race in genomics. The result is an engaging and insightful dialogue on questions that have vexed the field for decades. How do we-indeed "can" we reconcile the boundaries of biological and social difference? How do notions of "genetic ancestry" and "biogeographical ancestry differ from erstwhile racial and ethnic categories? Can racial categories ever be shorn of their colonial and oppressive legacies? Here we scrutinise the methodological and epistemological frameworks in contemporary genomics that work to define populations and shape our understanding of biology, society, health, and disease. We seek to clarify perspectives across the disciplinary divide, and to advance constructive and grounded critiques that contend with the question of justice in genomics., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2025 Schwartz-Marin, Jiwani, Abel, Egorova, M’charek, Meyer, Moreno Estrada, Schramm, Wade and Naslavsky.)

Introduction: The field of forensic DNA analysis has undergone rapid advancements in recent decades. The integration of massively parallel sequencing (MPS) has notably expanded the forensic toolkit, moving beyond identity matching to predicting phenotypic traits and biogeographical ancestry. This shift is of particular significance in cases where conventional DNA profiling fails to identify a single suspect. Supplementing forensic analyses with estimated biological age may be valuable but involves a complex and time-consuming DNA methylation analysis. This study explores and validates the performance of a comprehensive forensic third-generation sequencing assay utilizing Oxford Nanopore Technologies (ONT) in an adaptive and direct sequencing approach. We incorporated the most widely used forensic markers, i.e., STRs, SNPs, InDels, mitochondrial DNA (mtDNA), and two methylation-based clock classifiers, thereby combining forensic genetic and epigenetic analysis in one single workflow., Methods and Results: In our investigation, DNA from six anonymous individuals was sequenced using the ONT standard adaptive direct sequencing approach, reaching a mean percentage of on-target reads ranging from 6.6 % to 7.7 % per sample. ONT data was compared to standard MPS data and Illumina EPIC DNA methylation profiles. Basecalling employed recommended ONT software packages. TREAT was used for ONT-based analysis of autosomal and Y-chromosome STRs, achieving 90-92 % correct calls depending on allelic read depth thresholds. InDel analyses for two lower-quality samples proved challenging due to inadequate read depth, while the remaining four samples significantly contributed to the observed percentage markers (60.9 %) and correct calls (97.8 %). SNP analysis achieved a 98 % call rate, with only two mismatches and two missed alleles. ONT-generated DNA methylation data demonstrated Pearson's correlation coefficients with EPIC data ranging from 0.67 to 0.97 for Horvath's clock. Additional age-associated markers exhibited Pearson's correlation coefficients with chronological age between 0.14 (ELOVL2) and 0.96 (FHL2) at read depths of <30 and <20, respectively. Despite excluding mtDNA from our targeted sequencing approach, adaptive proof-reading fragments covered the complete mtDNA with an average read depth of 21-72, showing 100 % concordance with reference data., Discussion: Our exploratory study using ONT adaptive sequencing for conventional forensic and age associated DNA methylation markers showed high sequencing accuracy for a significant number of markers, showcasing ONT as a promising (epi)genetic forensic method. Future studies must address three critical aspects: determining clear quantity and quality measures and detection thresholds for accuracy, optimizing input DNA quantity for forensic casework expectations, and addressing ethical considerations associated with phenotype and ancestry analysis to prevent ethnic biases., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 The Authors. Published by Elsevier B.V. All rights reserved.)

What is race? And how does it figure in different scientific practices? To answer these questions, I suggest that we need to know race differently. Rather than defining race or looking for one conclusive answer to what it is, I propose methods that are open-ended, that allow us to follow race around, while remaining curious as to what it is. I suggest that we pursue generous methods . Drawing on empirical examples of forensic identification technologies, I argue that the slipperiness of race-the way race and its politics inexorably shift and change-cannot be fully grasped as an 'object multiple'. Race, I show, is not race: The same word refers to different phenomena. To grasp this, I introduce the notion of the affinity concept . Drawing on the history of race, along with contemporary work in forensic genetics, the affinity concept helps us articulate how race indexes three different scientific realities: race as object , race as method , and race as theory . These three different, yet interconnected realities, contribute to race's slipperiness as well as its virulence.

This special issue interrogates race through the lens of face. Its central faces are those in forensic settings. Promising immediate legibility and access to the individual suspect, forensic faces nevertheless mobilize a variety of collectives. We offer conceptual and methodological tools to examine the face as both an individual and a collective phenomenon, and demonstrate through detailed cases how the face thus allows us to address the absent presence of race. Given its long and convoluted history in physical anthropology, as a marker of racial typology, the face forces us to reckon with colonial histories of ordering human difference. But the face also allows us to question a regime of visuality that congeals around the face in Western culture. In this introduction to The Politics of Face and the Trouble with Race we elaborate these concerns and introduce the contributions to this volume.

The face, just like DNA, is taken to represent a unique individual. This article proposes to move beyond this representational model and to attend to the work that a face can do . I introduce the concept of tentacularity to capture the multiple works accomplished by the face. Drawing on the example of DNA phenotyping, which is used to produce a composite face of an unknown suspect, I first show that this novel technology does not so much produce the face of an individual suspect but that of a suspect population . Second, I demonstrate how the face draws the interest of diverse publics, who with their gaze flesh out its content and contours; the face engages and yields an affective response. I argue that the biologization of appearance by way of the face contributes to the racialization of populations. [ race, phenotype, material-semiotics, facial typologies, forensics genetics, DNA phenotyping ]., (© 2020 The Authors. American Anthropologist published by Wiley Periodicals, Inc. on behalf of American Anthropological Association.)

In 1999 a girl named Marianne Vaatstra was found murdered in a rural area in the Netherlands. In 2012 the perpetrator was arrested. Throughout this period as well as thereafter, the Vaatstra case was never far removed from media attention and public debate. How did this murder become such a high-profile case? In this article we employ the concept of the 'fire object' to examine the high-profileness of the Vaatstra case. Law and Singleton's fire metaphor helps to attend to objects as patterns of presences and absences. In the Vaatstra case it is in particular the unknown suspect that figures as a generative absence that brings to presence different versions of the case and allows them to proliferate. In this article we present four different versions of the Vaatstra case that were presented in the media and which shaped the identities of concerned actors. The unruly topology of fire objects, we argue, might well explain the high-profileness of such criminal cases., Competing Interests: Declaration of conflicting interests: The author(s) declared no potential conflicts of interest with respect to the research, authorship, and/or publication of this article.

DNA profiling is a well-established technology for use in the criminal justice system, both in courtrooms and elsewhere. The fact that DNA profiles are based on non-coding DNA and do not reveal details about the physical appearance of an individual has contributed to the acceptability of this type of evidence. Its success in criminal investigation, combined with major innovations in the field of genetics, have contributed to a change of role for this type of evidence. Nowadays DNA evidence is not merely about identification, where trace evidence is compared to a sample taken from a suspect. An ever-growing role is anticipated for DNA profiling as an investigative tool, a technique aimed at generating a suspect where there is none. One of these applications is the inference of visible traits. As this article will show, racial classifications are at the heart of this application. The Netherlands and its legal regulation of 'externally visible traits' will serve as an example. It will be shown that, to make this technology work, a large number of actors has to be enrolled and their articulations invited. This indicates that instead of a 'silent witness', a DNA profile should rather be seen as an 'articulate collective'. Based on two cases, I argue that the normativity of visible traits is context-dependent. Taking into account the practices in which technology is put to use alerts us to novel ethical questions raised by their application.