27 results on '"Ouyang, Xuesong"'
Search Results
2. A living biobank of matched pairs of patient-derived xenografts and organoids for cancer pharmacology.
- Author
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Xu X, Kumari R, Zhou J, Chen J, Mao B, Wang J, Zheng M, Tu X, An X, Chen X, Zhang L, Tian X, Wang H, Dong X, Bao Z, Guo S, Ouyang X, Shang L, Wang F, Yan X, Zhang R, Vries RGJ, Clevers H, and Li QX
- Subjects
- Animals, Humans, Biological Specimen Banks, Heterografts, Disease Models, Animal, Organoids, Xenograft Model Antitumor Assays, Neoplasms drug therapy, Neoplasms genetics, Neoplasms pathology, Antineoplastic Agents pharmacology
- Abstract
Patient-derived tumor xenograft (PDX)/organoid (PDO), driven by cancer stem cells (CSC), are considered the most predictive models for translational oncology. Large PDX collections reflective of patient populations have been created and used extensively to test various investigational therapies, including population-trials as surrogate subjects in vivo. PDOs are recognized as in vitro surrogates for patients amenable for high-throughput screening (HTS). We have built a biobank of carcinoma PDX-derived organoids (PDXOs) by converting an existing PDX library and confirmed high degree of similarities between PDXOs and parental PDXs in genomics, histopathology and pharmacology, suggesting "biological equivalence or interchangeability" between the two. Here we demonstrate the applications of PDXO biobank for HTS "matrix" screening for both lead compounds and indications, immune cell co-cultures for immune-therapies and engineering enables in vitro/in vivo imaging. This large biobank of >550 matched pairs of PDXs/PDXOs across different cancers could become powerful tools for the future cancer drug discovery., Competing Interests: X.X., J.Z., J.C., M.Z., J.W., X.T., X.A., X.C., L.Z., Z.B., L.S., F.W., X.Y, R.Z., S.G., B.M., X.O., R.K. and Q-X.L. are employees of Crown Bioscience, Inc; H.C. is inventor on several patents related to organoid technology; R.G.J.V. is an employee of HUB; X.T. is a full-time employee of Shanghai Yihao Biological Technology; H.W. and X.D are full-time employees of Suzhou Neologics Bioscience Co, LTD., (Copyright: © 2023 Xu et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.)
- Published
- 2023
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3. Different syngeneic tumors show distinctive intrinsic tumor-immunity and mechanisms of actions (MOA) of anti-PD-1 treatment.
- Author
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Jin Y, An X, Mao B, Sun R, Kumari R, Chen X, Shan Y, Zang M, Xu L, Muntel J, Beeler K, Bruderer R, Reiter L, Guo S, Zhou D, Li QX, and Ouyang X
- Subjects
- Animals, CD8-Positive T-Lymphocytes, Cell Line, Tumor, Humans, Lymphocytes, Tumor-Infiltrating, Mice, T-Lymphocytes, Regulatory, Tumor Microenvironment, Antineoplastic Agents metabolism, Immunotherapy
- Abstract
Cancers are immunologically heterogeneous. A range of immunotherapies target abnormal tumor immunity via different mechanisms of actions (MOAs), particularly various tumor-infiltrate leukocytes (TILs). We modeled loss of function (LOF) in four common anti-PD-1 antibody-responsive syngeneic tumors, MC38, Hepa1-6, CT-26 and EMT-6, by systematical depleting a series of TIL lineages to explore the mechanisms of tumor immunity and treatment. CD8
+ -T-cells, CD4+ -T-cells, Treg , NK cells and macrophages were individually depleted through either direct administration of anti-marker antibodies/reagents or using DTR (diphtheria toxin receptor) knock-in mice, for some syngeneic tumors, where specific subsets were depleted following diphtheria toxin (DT) administration. These LOF experiments revealed distinctive intrinsic tumor immunity and thus different MOAs in their responses to anti-PD-1 antibody among different syngeneic tumors. Specifically, the intrinsic tumor immunity and the associated anti-PD-1 MOA were predominately driven by CD8+ cytotoxic TILs (CTL) in all syngeneic tumors, excluding Hepa1-6 where CD4+ Teff TILs played a key role. TIL-Treg also played a critical role in supporting tumor growth in all four syngeneic models as well as M2 -macrophages. Pathway analysis using pharmacodynamic readouts of immuno-genomics and proteomics on MC38 and Hepa1-6 also revealed defined, but distinctive, immune pathways of activation and suppression between the two, closely associated with the efficacy and consistent with TIL-pharmacodynamic readouts. Understanding tumor immune-pathogenesis and treatment MOAs in the different syngeneic animal models, not only assists the selection of the right model for evaluating new immunotherapy of a given MOA, but also can potentially help to understand the potential disease mechanisms and strategize optimal immune-therapies in patients., (© 2022. The Author(s).)- Published
- 2022
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4. Preclinical pharmacology modeling of chimeric antigen receptor T therapies.
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Kumari R, Ouyang X, Wang J, Xu X, Zheng M, An X, and Li QX
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- Animals, Immunotherapy, Adoptive, Mice, Receptors, Antigen, T-Cell genetics, T-Lymphocytes, Tumor Microenvironment, Neoplasms drug therapy, Receptors, Chimeric Antigen
- Abstract
Chimeric antigen receptor (CAR) T cells have largely been successful in treating hematological malignancies in the clinic but have not been as effective in treating solid tumors, in part, owing to poor access and the immunosuppressive tumor microenvironment. In addition, CAR-T therapy can cause potentially life-threatening side effects, including cytokine release syndrome and neurotoxicity. Current preclinical testing of CAR-T therapy efficacy is typically performed in mouse tumor models, which often fails to predict toxicity. Recent developments in humanized models and transgenic mice as well as in vitro three-dimensional organoids in early development and nonhuman primate models are being adopted for CAR-T cell efficacy and toxicity assessment. However, because no single model perfectly recapitulates the human immune system and tumor microenvironment, careful model selection based on their respective pros and cons is crucial for adequate evaluation of different CAR-T treatments, so that their clinical development can be better supported., (Copyright © 2021. Published by Elsevier Ltd.)
- Published
- 2021
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5. Creating Matched In vivo/In vitro Patient-Derived Model Pairs of PDX and PDX-Derived Organoids for Cancer Pharmacology Research.
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Xu X, Shang L, Wang P, Zhou J, Ouyang X, Zheng M, Mao B, Zhang L, Chen B, Wang J, Chen J, Qian W, Guo S, Huang Y, and Li QX
- Subjects
- Animals, Disease Models, Animal, Humans, Mice, Pharmacology, Antineoplastic Agents, Neoplasms, Organoids, Xenograft Model Antitumor Assays
- Abstract
Patient-derived tumor xenografts (PDXs) are considered the most predictive preclinical models, largely believed to be driven by cancer stem cells (CSC) for conventional cancer drug evaluation. A large library of PDXs is reflective of the diversity of patient populations and thus enables population based preclinical trials ("Phase II-like mouse clinical trials"); however, PDX have practical limitations of low throughput, high costs and long duration. Tumor organoids, also being patient-derived CSC-driven models, can be considered as the in vitro equivalent of PDX, overcoming certain PDX limitations for dealing with large libraries of organoids or compounds. This study describes a method to create PDX-derived organoids (PDXO), thus resulting in paired models for in vitro and in vivo pharmacology research. Subcutaneously-transplanted PDX-CR2110 tumors were collected from tumor-bearing mice when the tumors reached 200-800 mm
3 , per an approved autopsy procedure, followed by removal of the adjacent non-tumor tissues and dissociation into small tumor fragments. The small tumor fragments were washed and passed through a 100 µm cell strainer to remove the debris. Cell clusters were collected and suspended in basement membrane extract (BME) solution and plated in a 6-well plate as a solid droplet with surrounding liquid media for growth in a CO2 incubator. Organoid growth was monitored twice weekly under light microscopy and recorded by photography, followed by liquid medium change 2 or 3 times a week. The grown organoids were further passaged (7 days later) at a 1:2 ratio by disrupting the BME embedded organoids using mechanical shearing, aided by addition of trypsin and the addition of 10 µM Y-27632. Organoids were cryopreserved in cryo-tubes for long-term storage, after release from BME by centrifugation, and also sampled (e.g., DNA, RNA and FFPE block) for further characterization.- Published
- 2021
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6. In Vivo Pharmacology Models for Cancer Target Research.
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Chen D, An X, Ouyang X, Cai J, Zhou D, and Li QX
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- Animals, Antineoplastic Agents therapeutic use, Carcinogenesis drug effects, Carcinogenesis genetics, Carcinogenesis pathology, Cell Line, Tumor, Humans, Mice, Molecular Targeted Therapy methods, Neoplasms genetics, Neoplasms pathology, Neoplasms, Experimental drug therapy, Neoplasms, Experimental genetics, Neoplasms, Experimental pathology, Transgenes, Tumor Burden drug effects, Antineoplastic Agents pharmacology, Drug Discovery methods, Gene Targeting methods, Neoplasms drug therapy, Xenograft Model Antitumor Assays methods
- Abstract
Experimental animal tumor models have been broadly used to evaluate anticancer drugs in the preclinical setting. They have also been widely applied for drug target discovery and validation, which usually follows four experimental strategies: first, assess the roles of putative drug targets using in vivo tumorigenicity and tumor growth kinetics assays of transplanted tumors, engineered through gain-of-function (GOF) by overexpressing transgene or knock-in (KI) or loss-of-function by gene silencing using knockdown (KD) or knockout (KO) or mutation via mutagenesis procedures; second, similarly genetically engineered mouse models (GEMM), through either germline or somatic cell procedures, are used to test the roles of potential targets in spontaneous tumorigenicity assays; third, patient-derived xenografts (PDXs), which most closely resemble patient genetics and histopathology, are used in tumor inhibition assays for evaluating target-/pathway-specific inhibitors, including large and small molecules, thus assessing the drug target; and fourth, the targets can be assessed in population-based trials, mouse clinical trials (MCT), so that the validation can be generally meaningful as performed in human clinical trials. This chapter outlines the commonly used protocols in cancer drug target research: the first four sections describe four sets of different, specific pharmacology protocols used in the respective cancer modeling stages, with the last section summarizing the common protocols applicable to all four pharmacology modeling steps.
- Published
- 2019
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7. Immunophenotyping of Orthotopic Homograft (Syngeneic) of Murine Primary KPC Pancreatic Ductal Adenocarcinoma by Flow Cytometry.
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An X, Ouyang X, Zhang H, Li T, Huang YY, Li Z, Zhou D, and Li QX
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- Allografts, Animals, Flow Cytometry, Immunophenotyping, Mice, Inbred C57BL, Mice, Transgenic, Transplantation, Homologous, Tumor Microenvironment immunology, Adenocarcinoma immunology, Carcinoma, Pancreatic Ductal immunology, Pancreatic Neoplasms immunology
- Abstract
Homograft (syngeneic) tumors are the workhorse of today's immuno-oncology (I/O) preclinical research. The tumor microenvironment (TME), particularly its immune-components, is vital to the prognosis and prediction of treatment outcomes, especially those of immunotherapy. TME immune-components are composed of different subsets of tumor-infiltrating immune cells assessable by multi-color FACS. Pancreatic ductal adenocarcinoma (PDAC) is among the deadliest malignances lacking good treatment options, thus an urgent and unmet medical need. One important reason for its non-responsiveness to various therapies (chemo-, targeted, I/O) has been its abundant TME, consisting of fibroblasts and leukocytes that protect tumor cells from these therapies. Orthotopically implanted PDAC is believed to more accurately recapture the TME of human pancreatic cancers than conventional subcutaneous (SC) models. Homograft tumors (KPC) are transplants of mouse spontaneous PDAC originating from genetically engineered KPC-mice (Kras
G12D/+ /P53-/- /Pdx1-Cre) (KPC-GEMM). The primary tumor tissue is cut into small fragments (~2 mm3 ) and transplanted subcutaneously (SC) to the syngeneic recipients (C57BL/6, 7-9 weeks old). The homografts were then surgically orthotopically transplanted onto the pancreas of new C57BL/6 mice, along with SC-implantation, which reached tumor volumes of 300-1,000 mm3 by 17 days. Only tumors of 400-600 mm3 were harvested per approved autopsy procedure and cleaned to remove the adjacent non-tumor tissues. They were dissociated per protocol using a tissue dissociator into single-cell suspensions, followed by staining with designated panels of fluorescently-labeled antibodies for various markers of different immune cells (lymphoid, myeloid and NK, DCs). The stained samples were analyzed using multi-color FACS to determine numbers of immune cells of different lineages, as well as their relative percentage within tumors. The immune profiles of orthotopic tumors were then compared to those of SC tumors. The preliminary data demonstrated significantly elevated infiltrating TILs/TAMs in tumors over the pancreas, and higher B-cell infiltration into orthotopic rather than SC tumors.- Published
- 2018
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8. Inhibition of O-GlcNAcase leads to elevation of O-GlcNAc tau and reduction of tauopathy and cerebrospinal fluid tau in rTg4510 mice.
- Author
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Hastings NB, Wang X, Song L, Butts BD, Grotz D, Hargreaves R, Fred Hess J, Hong KK, Huang CR, Hyde L, Laverty M, Lee J, Levitan D, Lu SX, Maguire M, Mahadomrongkul V, McEachern EJ, Ouyang X, Rosahl TW, Selnick H, Stanton M, Terracina G, Vocadlo DJ, Wang G, Duffy JL, Parker EM, and Zhang L
- Subjects
- Animals, Mice, Mice, Transgenic, Protein Processing, Post-Translational, Pyrans pharmacology, Thiazoles pharmacology, Tauopathies metabolism, beta-N-Acetylhexosaminidases antagonists & inhibitors, tau Proteins metabolism
- Abstract
Background: Hyperphosphorylation of microtubule-associated protein tau is a distinct feature of neurofibrillary tangles (NFTs) that are the hallmark of neurodegenerative tauopathies. O-GlcNAcylation is a lesser known post-translational modification of tau that involves the addition of N-acetylglucosamine onto serine and threonine residues. Inhibition of O-GlcNAcase (OGA), the enzyme responsible for the removal of O-GlcNAc modification, has been shown to reduce tau pathology in several transgenic models. Clarifying the underlying mechanism by which OGA inhibition leads to the reduction of pathological tau and identifying translatable measures to guide human dosing and efficacy determination would significantly facilitate the clinical development of OGA inhibitors for the treatment of tauopathies., Methods: Genetic and pharmacological approaches are used to evaluate the pharmacodynamic response of OGA inhibition. A panel of quantitative biochemical assays is established to assess the effect of OGA inhibition on pathological tau reduction. A "click" chemistry labeling method is developed for the detection of O-GlcNAcylated tau., Results: Substantial (>80%) OGA inhibition is required to observe a measurable increase in O-GlcNAcylated proteins in the brain. Sustained and substantial OGA inhibition via chronic treatment with Thiamet G leads to a significant reduction of aggregated tau and several phosphorylated tau species in the insoluble fraction of rTg4510 mouse brain and total tau in cerebrospinal fluid (CSF). O-GlcNAcylated tau is elevated by Thiamet G treatment and is found primarily in the soluble 55 kD tau species, but not in the insoluble 64 kD tau species thought as the pathological entity., Conclusion: The present study demonstrates that chronic inhibition of OGA reduces pathological tau in the brain and total tau in the CSF of rTg4510 mice, most likely by directly increasing O-GlcNAcylation of tau and thereby maintaining tau in the soluble, non-toxic form by reducing tau aggregation and the accompanying panoply of deleterious post-translational modifications. These results clarify some conflicting observations regarding the effects and mechanism of OGA inhibition on tau pathology, provide pharmacodynamic tools to guide human dosing and identify CSF total tau as a potential translational biomarker. Therefore, this study provides additional support to develop OGA inhibitors as a treatment for Alzheimer's disease and other neurodegenerative tauopathies.
- Published
- 2017
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9. Experimental animal modeling for immuno-oncology.
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Li QX, Feuer G, Ouyang X, and An X
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- Animals, Drug Design, Drug Discovery methods, Humans, Molecular Targeted Therapy, Neoplasms immunology, Neoplasms pathology, Disease Models, Animal, Immunotherapy methods, Neoplasms drug therapy
- Abstract
Immuno-oncology (I/O) research has intensified significantly in recent years due to the breakthrough development and the regulatory approval of several immune checkpoint inhibitors, leading to the rapid expansion of the new discovery of novel I/O therapies, new checkpoint inhibitors and beyond. However, many I/O questions remain unanswered, including why only certain subsets of patients respond to these treatments, who the responders would be, and how to expand patient response (the conversion of non-responders or maximizing response in partial responders). All of these require relevant I/O experimental systems, particularly relevant preclinical animal models. Compared to other oncology drug discovery, e.g. cytotoxic and targeted drugs, a lack of relevant animal models is a major obstacle in I/O drug discovery, and an urgent and unmet need. Despite the obvious importance, and the fact that much I/O research has been performed using many different animal models, there are few comprehensive and introductory reviews on this topic. This article attempts to review the efforts in development of a variety of such models, as well as their applications and limitations for readers new to the field, particularly those in the pharmaceutical industry., (Copyright © 2017. Published by Elsevier Inc.)
- Published
- 2017
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10. Nuclear Export of Ubiquitinated Proteins Determines the Sensitivity of Colorectal Cancer to Proteasome Inhibitor.
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Wu T, Chen W, Zhong Y, Hou X, Fang S, Liu CY, Wang G, Yu T, Huang YY, Ouyang X, Li HQ, Cui L, and Yang Y
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- Active Transport, Cell Nucleus drug effects, Animals, Antineoplastic Agents pharmacology, Bortezomib pharmacology, Cell Line, Tumor, Cell Survival drug effects, Colorectal Neoplasms metabolism, Drug Synergism, HCT116 Cells, HeLa Cells, Humans, Hydrazines administration & dosage, Hydrazines pharmacology, Mice, Proteasome Inhibitors pharmacology, Triazoles administration & dosage, Triazoles pharmacology, Ubiquitination drug effects, Xenograft Model Antitumor Assays, Antineoplastic Agents administration & dosage, Bortezomib administration & dosage, Cell Nucleus metabolism, Colorectal Neoplasms drug therapy, Proteasome Inhibitors administration & dosage, Tumor Suppressor Protein p53 metabolism
- Abstract
Although proteasome inhibitors such as bortezomib had significant therapeutic effects in multiple myeloma and mantel cell lymphoma, they exhibited minimal clinical activity as a monotherapy for solid tumors, including colorectal cancer. We found in this study that proteasome inhibition induced a remarkable nuclear exportation of ubiquitinated proteins. Inhibition of CRM1, the nuclear export carrier protein, hampered protein export and synergistically enhanced the cytotoxic action of bortezomib on colon cancer cells containing wild-type p53, which underwent G
2 -M cell-cycle block and apoptosis. Further analysis indicated that tumor suppressor p53 was one of the proteins exported from nuclei upon proteasome inhibition, and in the presence of CRM1 inhibitor KPT330, nuclear p53, and expression of its target genes were increased markedly. Moreover, knockdown of p53 significantly reduced the synergistic cytotoxic action of bortezomib and KPT330 on p53+/+ HCT116 cells. In mice, KPT330 markedly augmented the antitumor action of bortezomib against HCT116 xenografts as well as patient-derived xenografts that harbored functional p53. These results indicate that nuclear p53 is a major mediator in the synergistic antitumor effect of bortezomib and KPT330, and provides a rationale for the use of proteasome inhibitor together with nuclear export blocker in the treatment of colorectal cancer. It is conceivable that targeting nuclear exportation may serve as a novel strategy to overcome resistance and raise chemotherapeutic efficacy, especially for the drugs that activate the p53 system. Mol Cancer Ther; 16(4); 717-28. ©2016 AACR ., (©2016 American Association for Cancer Research.)- Published
- 2017
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11. Identification of an NKX3.1-G9a-UTY transcriptional regulatory network that controls prostate differentiation.
- Author
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Dutta A, Le Magnen C, Mitrofanova A, Ouyang X, Califano A, and Abate-Shen C
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- Animals, Cell Line, Epithelial Cells, Gene Knockdown Techniques, Histocompatibility Antigens, Histone-Lysine N-Methyltransferase, Homeodomain Proteins, Humans, Lentivirus, Male, Mice, Minor Histocompatibility Antigens, Nuclear Proteins, Prostate, Rats, Transcription Factors, Gene Regulatory Networks
- Abstract
The NKX3.1 homeobox gene plays essential roles in prostate differentiation and prostate cancer. We show that loss of function of Nkx3.1 in mouse prostate results in down-regulation of genes that are essential for prostate differentiation, as well as up-regulation of genes that are not normally expressed in prostate. Conversely, gain of function of Nkx3.1 in an otherwise fully differentiated nonprostatic mouse epithelium (seminal vesicle) is sufficient for respecification to prostate in renal grafts in vivo. In human prostate cells, these activities require the interaction of NKX3.1 with the G9a histone methyltransferase via the homeodomain and are mediated by activation of target genes such as UTY (KDM6c), the male-specific paralog of UTX (KDM6a) We propose that an NKX3.1-G9a-UTY transcriptional regulatory network is essential for prostate differentiation, and we speculate that disruption of such a network predisposes to prostate cancer., (Copyright © 2016, American Association for the Advancement of Science.)
- Published
- 2016
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12. Analysis of tau post-translational modifications in rTg4510 mice, a model of tau pathology.
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Song L, Lu SX, Ouyang X, Melchor J, Lee J, Terracina G, Wang X, Hyde L, Hess JF, Parker EM, and Zhang L
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- Animals, Biomarkers analysis, Brain pathology, Disease Models, Animal, Humans, Mice, Transgenic, Protein Processing, Post-Translational physiology, Tauopathies genetics, Brain metabolism, Protein Processing, Post-Translational genetics, Tauopathies metabolism, tau Proteins genetics, tau Proteins metabolism
- Abstract
Background: Microtubule associated protein tau is the major component of the neurofibrillary tangles (NFTs) found in the brains of patients with Alzheimer's disease and several other neurodegenerative diseases. Tau mutations are associated with frontotemperal dementia with parkinsonism on chromosome 17 (FTDP-17). rTg4510 mice overexpress human tau carrying the P301L FTDP-17 mutation and develop robust NFT-like pathology at 4-5 months of age. The current study is aimed at characterizing the rTg4510 mice to better understand the genesis of tau pathology and to better enable the use of this model in drug discovery efforts targeting tau pathology., Results: Using a panel of immunoassays, we analyzed the age-dependent formation of pathological tau in rTg4510 mice and our data revealed a steady age-dependent accumulation of pathological tau in the insoluble fraction of brain homogenates. The pathological tau was associated with multiple post-translational modifications including aggregation, phosphorylation at a wide variety of sites, acetylation, ubiquitination and nitration. The change of most tau species reached statistical significance at the age of 16 weeks. There was a strong correlation between the different post-translationally modified tau species in this heterogeneous pool of pathological tau. Total tau in the cerebrospinal fluid (CSF) displayed a multiphasic temporal profile distinct from the steady accumulation of pathological tau in the brain. Female rTg4510 mice displayed significantly more aggressive accumulation of pathological tau in the brain and elevation of total tau in CSF than their male littermates., Conclusion: The immunoassays described here were used to generate the most comprehensive description of the changes in various tau species across the lifespan of the rTg4510 mouse model. The data indicate that development of tauopathy in rTg4510 mice involves the accumulation of a pool of pathological tau that carries multiple post-translational modifications, a process that can be detected well before the histological detection of NFTs. Therapeutic treatment targeting tau should therefore aim to reduce all tau species associated with the pathological tau pool rather than reduce specific post-translational modifications. There is still much to learn about CSF tau in physiological and pathological processes in order to use it as a translational biomarker in drug discovery.
- Published
- 2015
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13. Diacylglycerol acyltransferase-1 (DGAT1) inhibition perturbs postprandial gut hormone release.
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Lin HV, Chen D, Shen Z, Zhu L, Ouyang X, Vongs A, Kan Y, Levorse JM, Kowalik EJ Jr, Szeto DM, Yao X, Xiao J, Chen S, Liu J, Garcia-Calvo M, Shin MK, and Pinto S
- Subjects
- Animals, Base Sequence, Diacylglycerol O-Acyltransferase deficiency, Diacylglycerol O-Acyltransferase metabolism, Diet, Dipeptidyl Peptidase 4 genetics, Dipeptidyl Peptidase 4 metabolism, Dogs, Enzyme Activation, Female, Gastric Emptying genetics, Gene Dosage, Gene Expression Regulation, Gene Order, Genotype, Glucagon-Like Peptide 1 metabolism, Lipid Metabolism, Male, Mice, Mice, Knockout, Molecular Sequence Data, Triglycerides blood, Diacylglycerol O-Acyltransferase genetics, Gastrointestinal Hormones metabolism, Gastrointestinal Tract metabolism, Postprandial Period
- Abstract
Diacylglycerol acyltransferase-1 (DGAT1) is a potential therapeutic target for treatment of obesity and related metabolic diseases. However, the degree of DGAT1 inhibition required for metabolic benefits is unclear. Here we show that partial DGAT1 deficiency in mice suppressed postprandial triglyceridemia, led to elevations in glucagon-like peptide-1 (GLP-1) and peptide YY (PYY) only following meals with very high lipid content, and did not protect from diet-induced obesity. Maximal DGAT1 inhibition led to enhanced GLP-1 and PYY secretion following meals with physiologically relevant lipid content. Finally, combination of DGAT1 inhibition with dipeptidyl-peptidase-4 (DPP-4) inhibition led to further enhancements in active GLP-1 in mice and dogs. The current study suggests that targeting DGAT1 to enhance postprandial gut hormone secretion requires maximal inhibition, and suggests combination with DPP-4i as a potential strategy to develop DGAT1 inhibitors for treatment of metabolic diseases.
- Published
- 2013
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14. Attenuation of Slc27a5 gene expression followed by LC-MS measurement of bile acid reconjugation using metabolomics and a stable isotope tracer strategy.
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Castro-Perez JM, Roddy TP, Shah V, Wang SP, Ouyang X, Ogawa A, McLaren DG, Tadin-Strapps M, Robinson MJ, Bartz SR, Ason B, Chen Y, Previs SF, Wong KK, Vreeken RJ, Johns DG, Hubbard BK, Hankemeier T, and Mitnaul L
- Subjects
- Animals, Gene Silencing, Humans, Mice, Mice, Inbred C57BL, Mice, Transgenic, Spectrometry, Mass, Electrospray Ionization, Bile Acids and Salts metabolism, Chromatography, Liquid methods, Fatty Acid Transport Proteins metabolism, Liver pathology, Mass Spectrometry methods, Metabolomics methods
- Abstract
The purpose of this study was to evaluate the use of high resolution LC-MS together with metabolomics and D(4)-cholic acid (D(4)-CA) as a metabolic tracer to measure the metabolism and reconjugation of bile acids (BAs) in vitro and in vivo. Metabolic tracers are very important because they allow for the direct detection (substrate-to-product) of small and significant biological perturbations that may not be apparent when monitoring "static" endogenous levels of particular metabolites. Slc27a5, also known as fatty acid transport protein 5 (FATP5), is the hepatic BA-CoA ligase involved in reconjugating BAs during enterohepatic BA recycling. Using Slc27a5-cKD mice, silencing of ∼90% gene expression was achieved followed by reduction in the reconjugation of D(4)-CA to D(4)-taurocholic acid (D(4)-TCA), as well as other conjugated BA metabolites in plasma (p = 0.0031). The method described allowed a rapid measure of many D(4) and endogenous BA. Analysis of bile resulted in the detection of 39 BA metabolites from a 13 min analytical run. Finally, the utilization of a novel high resolution mass spectrometry method in combination with metabolomics and a stable isotope metabolic tracer allowed for the detection of targeted and untargeted BAs following silencing of the Slc27a5 gene in primary hepatocytes and in mice.
- Published
- 2011
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15. Targeting AKT/mTOR and ERK MAPK signaling inhibits hormone-refractory prostate cancer in a preclinical mouse model.
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Kinkade CW, Castillo-Martin M, Puzio-Kuter A, Yan J, Foster TH, Gao H, Sun Y, Ouyang X, Gerald WL, Cordon-Cardo C, and Abate-Shen C
- Subjects
- Animals, Antineoplastic Agents pharmacology, Disease Models, Animal, Dose-Response Relationship, Drug, Enzyme Inhibitors pharmacology, Hormones metabolism, Humans, MAP Kinase Signaling System, Male, Mice, Models, Biological, Prostatic Neoplasms pathology, TOR Serine-Threonine Kinases, Extracellular Signal-Regulated MAP Kinases metabolism, Prostatic Neoplasms metabolism, Protein Kinases metabolism, Proto-Oncogene Proteins c-akt metabolism
- Abstract
The AKT/mammalian target of rapamycin (AKT/mTOR) and ERK MAPK signaling pathways have been shown to cooperate in prostate cancer progression and the transition to androgen-independent disease. We have now tested the effects of combinatorial inhibition of these pathways on prostate tumorigenicity by performing preclinical studies using a genetically engineered mouse model of prostate cancer. We report here that combination therapy using rapamycin, an inhibitor of mTOR, and PD0325901, an inhibitor of MAPK kinase 1 (MEK; the kinase directly upstream of ERK), inhibited cell growth in cultured prostate cancer cell lines and tumor growth particularly for androgen-independent prostate tumors in the mouse model. We further showed that such inhibition leads to inhibition of proliferation and upregulated expression of the apoptotic regulator Bcl-2-interacting mediator of cell death (Bim). Furthermore, analyses of human prostate cancer tissue microarrays demonstrated that AKT/mTOR and ERK MAPK signaling pathways are often coordinately deregulated during prostate cancer progression in humans. We therefore propose that combination therapy targeting AKT/mTOR and ERK MAPK signaling pathways may be an effective treatment for patients with advanced prostate cancer, in particular those with hormone-refractory disease.
- Published
- 2008
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16. Activator protein-1 transcription factors are associated with progression and recurrence of prostate cancer.
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Ouyang X, Jessen WJ, Al-Ahmadie H, Serio AM, Lin Y, Shih WJ, Reuter VE, Scardino PT, Shen MM, Aronow BJ, Vickers AJ, Gerald WL, and Abate-Shen C
- Subjects
- Animals, Disease Models, Animal, Disease Progression, Enzyme Activation, Epidermal Growth Factor metabolism, Gene Expression Profiling, Gene Expression Regulation, Neoplastic genetics, Homeodomain Proteins genetics, MAP Kinase Signaling System, Male, Mice, Mice, Mutant Strains, Mitogen-Activated Protein Kinase Kinases metabolism, Neoplasm Recurrence, Local genetics, Neoplasm Recurrence, Local metabolism, Neoplasm Recurrence, Local pathology, Oncogene Protein p65(gag-jun) biosynthesis, Oncogene Protein p65(gag-jun) metabolism, PTEN Phosphohydrolase genetics, Prostatic Neoplasms metabolism, Proto-Oncogene Proteins c-fos biosynthesis, Proto-Oncogene Proteins c-fos metabolism, Transcription Factor AP-1 biosynthesis, Transcription Factor AP-1 metabolism, Transcription Factors genetics, Oncogene Protein p65(gag-jun) genetics, Prostatic Neoplasms genetics, Prostatic Neoplasms pathology, Proto-Oncogene Proteins c-fos genetics, Transcription Factor AP-1 genetics
- Abstract
To identify biomarkers that discriminate the aggressive forms of prostate cancer, we performed gene expression profiling of prostate tumors using a genetically engineered mouse model that recapitulates the stages of human prostate cancer, namely Nkx3.1; Pten mutant mice. We observed a significant deregulation of the epidermal growth factor and mitogen-activated protein kinase (MAPK) signaling pathways, as well as their major downstream effectors--the activator protein-1 transcription factors c-Fos and c-Jun. Forced expression of c-Fos and c-Jun in prostate cancer cells promotes tumorigenicity and results in activation of extracellular signal-regulated kinase (Erk) MAPK signaling. In human prostate cancer, up-regulation of c-Fos and c-Jun proteins occurs in advanced disease and is correlated with Erk MAPK pathway activation, whereas high levels of c-Jun expression are associated with disease recurrence. Our analyses reveal a hitherto unappreciated role for AP-1 transcription factors in prostate cancer progression and identify c-Jun as a marker of high-risk prostate cancer. This study provides a striking example of how accurate mouse models can provide insights on molecular processes involved in progression and recurrence of human cancer.
- Published
- 2008
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17. BRAF activation initiates but does not maintain invasive prostate adenocarcinoma.
- Author
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Jeong JH, Wang Z, Guimaraes AS, Ouyang X, Figueiredo JL, Ding Z, Jiang S, Guney I, Kang GH, Shin E, Hahn WC, Loda MF, Abate-Shen C, Weissleder R, and Chin L
- Subjects
- Androgens, Animals, Biomarkers, Tumor metabolism, Castration, Cell Lineage, Cell Proliferation, Epithelial Cells enzymology, Epithelial Cells pathology, Gene Expression Regulation, Neoplastic, Humans, Hyperplasia, Male, Mice, Mice, Transgenic, Neoplasm Invasiveness, Phosphoproteins metabolism, Prostate enzymology, Prostate pathology, Prostatic Neoplasms enzymology, Prostatic Neoplasms genetics, Proto-Oncogene Proteins B-raf genetics, Proto-Oncogene Proteins c-akt metabolism, Rats, Trans-Activators metabolism, Transgenes, Urothelium embryology, Urothelium pathology, Prostatic Neoplasms pathology, Proto-Oncogene Proteins B-raf metabolism
- Abstract
Prostate cancer is the second leading cause of cancer-related deaths in men. Activation of MAP kinase signaling pathway has been implicated in advanced and androgen-independent prostate cancers, although formal genetic proof has been lacking. In the course of modeling malignant melanoma in a tyrosinase promoter transgenic system, we developed a genetically-engineered mouse (GEM) model of invasive prostate cancers, whereby an activating mutation of BRAF(V600E)--a mutation found in approximately 10% of human prostate tumors--was targeted to the epithelial compartment of the prostate gland on the background of Ink4a/Arf deficiency. These GEM mice developed prostate gland hyperplasia with progression to rapidly growing invasive adenocarcinoma without evidence of AKT activation, providing genetic proof that activation of MAP kinase signaling is sufficient to drive prostate tumorigenesis. Importantly, genetic extinction of BRAF(V600E) in established prostate tumors did not lead to tumor regression, indicating that while sufficient to initiate development of invasive prostate adenocarcinoma, BRAF(V600E) is not required for its maintenance.
- Published
- 2008
- Full Text
- View/download PDF
18. Prolonged exposure to reduced levels of androgen accelerates prostate cancer progression in Nkx3.1; Pten mutant mice.
- Author
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Banach-Petrosky W, Jessen WJ, Ouyang X, Gao H, Rao J, Quinn J, Aronow BJ, and Abate-Shen C
- Subjects
- Androgens metabolism, Animals, Disease Progression, Male, Mice, Mice, Inbred C57BL, Neoplasms, Hormone-Dependent metabolism, Neoplasms, Hormone-Dependent pathology, Orchiectomy, Prostatic Neoplasms metabolism, Prostatic Neoplasms pathology, Testosterone Propionate pharmacology, Androgens deficiency, Homeodomain Proteins genetics, Neoplasms, Hormone-Dependent genetics, PTEN Phosphohydrolase genetics, Prostatic Neoplasms genetics, Transcription Factors genetics
- Abstract
In this report, we have investigated the relationship between androgen levels and prostate tumorigenesis in Nkx3.1; Pten mutant mice, a genetically engineered mouse model of human prostate cancer. By experimentally manipulating serum levels of testosterone in these mice for an extended period (i.e., 7 months), we have found that prolonged exposure of Nkx3.1; Pten mutant mice to androgen levels that are 10-fold lower than normal (the "Low-T" group) resulted in a marked acceleration of prostate tumorigenesis compared with those exposed to androgen levels within the reference range (the "Normal-T" group). We found that prostate tumors from the Low-T mutant mice share a similar gene expression profile as androgen-independent prostate tumors from these mutant mice, which includes the deregulated expression of several genes that are up-regulated in human hormone-refractory prostate cancer, such as Vav3 and Runx1. We propose that exposure to reduced androgens may promote prostate tumorigenesis by selecting for molecular events that promote more aggressive, hormone-refractory tumors.
- Published
- 2007
- Full Text
- View/download PDF
19. Vitamin D inhibits the formation of prostatic intraepithelial neoplasia in Nkx3.1;Pten mutant mice.
- Author
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Banach-Petrosky W, Ouyang X, Gao H, Nader K, Ji Y, Suh N, DiPaola RS, and Abate-Shen C
- Subjects
- Adenocarcinoma genetics, Adenocarcinoma pathology, Animals, Bone Density Conservation Agents therapeutic use, Disease Models, Animal, Homeodomain Proteins genetics, Male, Mice, Mice, Inbred C57BL, Mice, Mutant Strains, Mice, Nude, PTEN Phosphohydrolase genetics, Prostatic Intraepithelial Neoplasia pathology, Prostatic Neoplasms genetics, Transcription Factors genetics, Homeodomain Proteins physiology, Mutation, PTEN Phosphohydrolase physiology, Precancerous Conditions prevention & control, Prostatic Intraepithelial Neoplasia prevention & control, Prostatic Neoplasms pathology, Transcription Factors physiology, Vitamin D therapeutic use
- Abstract
Purpose: Epidemiologic studies have shown that reduced levels of vitamin D represent a major risk factor for prostate cancer. In this report, we have examined the efficacy of 1alpha,25-dihydroxyvitamin D(3) (1,25 D(3)) as a chemopreventive agent using Nkx3.1; Pten mutant mice, which recapitulate stages of prostate carcinogenesis from prostate intraepithelial neoplasia (PIN) to adenocarcinoma., Experimental Design: 1,25 D(3) (or vehicle) was delivered continuously to Nkx3.1; Pten mutant or control mice for a 4-month period beginning before (precancerous cohort) or after (cancerous cohort) these mice developed PIN. At the conclusion of the study, the mice were analyzed for the occurrence of PIN and/or cancer phenotypes by histologic analyses and immunostaining using known markers of cancer progression in these mice., Results: We found that sustained delivery of 1,25 D(3) to the Nkx3.1; Pten mutant mice resulted in a significant reduction in the formation of PIN while having no apparent effect on the control mice. Furthermore, 1,25 D(3) was maximally effective when delivered before, rather than subsequent to, the initial occurrence of PIN. We further show that this 1,25 D(3)-mediated inhibition of PIN was coincident with up-regulation of vitamin D receptor expression in the prostatic epithelium of the mutant mice, as well as in CASP prostate epithelial cell lines developed from these mice, while having no effect on androgen receptor expression or androgen receptor signaling., Conclusion: Our findings show the value of chemoprevention studies using Nkx3.1; Pten mutant mice, particularly for evaluating the efficacy and underlying mechanisms of potential agents and to gain insights about the optimal timing of their delivery. In particular, our study predicts that vitamin D may have differential effects during early-stage versus late-stage disease and that it is more likely to be beneficial if delivered either before the overt manifestation of clinically detectable disease or during the earliest disease stages, rather than in advanced disease. Thus, our findings support the assessment of vitamin D analogues for chemoprevention in clinical trials targeting patients with early-stage disease and also establish molecular markers that can be used in such trials to determine biological activity and to optimize further clinical trials.
- Published
- 2006
- Full Text
- View/download PDF
20. Combinatorial activities of Akt and B-Raf/Erk signaling in a mouse model of androgen-independent prostate cancer.
- Author
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Gao H, Ouyang X, Banach-Petrosky WA, Gerald WL, Shen MM, and Abate-Shen C
- Subjects
- Animals, Cells, Cultured, Disease Models, Animal, Enzyme Activation, Male, Mice, Mice, Mutant Strains, PTEN Phosphohydrolase deficiency, Prostate cytology, Prostate pathology, Rats, Receptors, Androgen metabolism, Androgens metabolism, Extracellular Signal-Regulated MAP Kinases metabolism, Prostatic Neoplasms pathology, Proto-Oncogene Proteins B-raf metabolism, Proto-Oncogene Proteins c-akt metabolism, Signal Transduction
- Abstract
Androgen independence is responsible for most prostate cancer lethality, yet currently there are no effective clinical treatments. We have been investigating the mechanisms underlying androgen-independent prostate cancer in Nkx3.1;Pten mutant mice, which display salient features of the disease, including a requirement for wild-type androgen receptor (AR) signaling. We now demonstrate that the Akt and Erk MAP kinase signaling pathways are activated in androgen-independent lesions of these mice. Forced activation of either Akt or Erk signaling in an androgen-responsive prostate cancer cell line promotes hormone-independent but AR-dependent growth in culture. Although these pathways act additively in culture, they act synergistically in vivo to promote tumorigenicity and androgen independence in the context of the prostate microenvironment. We propose that androgen independence emerges by means of epithelial-stromal competition, in which activation of Akt and Erk promotes AR activity in the prostate epithelium while counteracting antagonistic effects of the stroma.
- Published
- 2006
- Full Text
- View/download PDF
21. Emergence of androgen independence at early stages of prostate cancer progression in Nkx3.1; Pten mice.
- Author
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Gao H, Ouyang X, Banach-Petrosky WA, Shen MM, and Abate-Shen C
- Subjects
- Aging, Androgens deficiency, Animals, Cell Division, Cell Survival, Epithelial Cells cytology, Male, Mice, Mice, Mutant Strains, Neoplasm Staging, Orchiectomy, Prostate cytology, Androgens physiology, Homeodomain Proteins genetics, Mutation, PTEN Phosphohydrolase genetics, Prostatic Neoplasms genetics, Prostatic Neoplasms pathology, Transcription Factors genetics
- Abstract
Although androgen deprivation therapy is a widely used treatment for patients with advanced prostate cancer, it ultimately results in the emergence of a hormone-refractory disease that is invariably fatal. To provide insights into the genesis of this disease, we have employed an in vivo model to investigate how and when prostate epithelial cells can acquire the ability to survive and proliferate in the absence of androgens. In particular, we have been studying the evolution of androgen independence in Nkx3.1; Pten mutant mice, which develop prostatic intraepithelial neoplasia and adenocarcinoma as a consequence of aging, as well as androgen-independent phenotypes following castration. We now find that the prostate epithelial cells from these Nkx3.1; Pten mutant mice are capable of surviving and proliferating in the absence of androgens and that they develop androgen-independent phenotypes well before they display overt prostatic intraepithelial neoplasia or cancer phenotypes. Our findings in this mouse model show that acquisition of androgen independence can be uncoupled from overt cancer progression and raise the possibility that hormone-refractory disease can arise at early stages of prostate carcinogenesis.
- Published
- 2006
- Full Text
- View/download PDF
22. Microarray and bioinformatic detection of novel and established genes expressed in experimental anti-Thy1 nephritis.
- Author
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Sadlier DM, Ouyang X, McMahon B, Mu W, Ohashi R, Rodgers K, Murray D, Nakagawa T, Godson C, Doran P, Brady HR, and Johnson RJ
- Subjects
- Animals, Cell Division drug effects, Cell Division genetics, Cells, Cultured, Cluster Analysis, Disease Models, Animal, Humans, Isoantibodies, Mesangial Cells cytology, Platelet-Derived Growth Factor pharmacology, Rats, Rats, Wistar, Reproducibility of Results, Reverse Transcriptase Polymerase Chain Reaction, Computational Biology, Glomerulonephritis, Membranoproliferative genetics, Mesangial Cells physiology, Oligonucleotide Array Sequence Analysis standards
- Abstract
Background: Microarray technology is a powerful tool that can probe the molecular pathogenesis of renal injury. In this present study microarray analysis was used to monitor serial changes in the renal transcriptome of a rat model of mesangial proliferative glomerulonephritis. Administration of anti-Thy1 antibody results in phases of acute mesangial injury (day 2), cell proliferation (day 5), matrix expansion (days 5 and 7), and subsequent healing (day 14)., Methods: Using Affymetrix (RAE230A) microarrays coupled with sequential primary biologic function-focused and secondary "baited" global cluster analysis, a cohort of established and putative novel modulators of mesangial cell turnover was identified., Results: Cluster analysis of proliferative genes identified a number of gene expression profiles. The most striking pattern was increased gene expression at day 5, a cluster that included platelet-derived growth factor (PDGF), cyclins and transforming growth factor-beta (TGF-beta). The gene expression patterns identified by primary focused cluster analysis were used as bioinformatic bait and resulted in the identification of novel families of genes such as the S100 family. The expression of established and novel genes was confirmed using reverse transcription-polymerase chain reaction (RT-PCR). Next, in vivo gene expression was compared to PDGF-stimulated mesangial cells in vitro revealing similar patterns of dysregulation., Conclusion: Transcriptomic analysis defined both known and novel molecules involved in mesangial cell proliferation in vitro and in vivo and defined a panel of molecules that are potential contributors to mesangial cell dysfunction in glomerular disease.
- Published
- 2005
- Full Text
- View/download PDF
23. Loss-of-function of Nkx3.1 promotes increased oxidative damage in prostate carcinogenesis.
- Author
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Ouyang X, DeWeese TL, Nelson WG, and Abate-Shen C
- Subjects
- Animals, Antioxidants metabolism, Cell Transformation, Neoplastic metabolism, DNA metabolism, DNA Damage, Gene Expression Profiling, Gene Expression Regulation, Gene Expression Regulation, Neoplastic, Glutathione Peroxidase biosynthesis, Glutathione Peroxidase genetics, Homeodomain Proteins genetics, Male, Mice, Mice, Inbred C57BL, Mutation, Oxidative Stress genetics, Peroxidases biosynthesis, Peroxidases genetics, Peroxiredoxin VI, Peroxiredoxins, Prostate enzymology, Prostate metabolism, Prostate physiology, Prostatic Neoplasms enzymology, Prostatic Neoplasms metabolism, Transcription Factors deficiency, Transcription Factors genetics, Cell Transformation, Neoplastic genetics, Homeodomain Proteins physiology, Prostatic Neoplasms genetics, Transcription Factors physiology
- Abstract
Despite the significance of oxidative damage for carcinogenesis, the molecular mechanisms that lead to increased susceptibility of tissues to oxidative stress are not well-understood. We now report a link between loss of protection against oxidative damage and loss-of-function of Nkx3.1, a homeobox gene that is known to be required for prostatic epithelial differentiation and suppression of prostate cancer. Using gene expression profiling, we find that Nkx3.1 mutant mice display deregulated expression of several antioxidant and prooxidant enzymes, including glutathione peroxidase 2 and 3 (GPx2 and GPx3), peroxiredoxin 6 (Prdx6), and sulfyhydryl oxidase Q6 (Qscn6). Moreover, the formation of prostatic intraepithelial neoplasia in these mutant mice is associated with increased oxidative damage of DNA, as evident by increased levels of 8-hydroxy-2'-deoxyguanosine. We further show that progression to prostate adenocarcinoma, as occurs in compound mutant mice lacking Nkx3.1 as well as the Pten tumor suppressor, is correlated with a further deregulation of antioxidants, including superoxide dismutase enzymes, and more profound accumulations of oxidative damage to DNA and protein, the latter manifested by increased levels of 4-hydroxynonenal. We propose that the essential role of Nkx3.1 in maintaining the terminally differentiated state of the prostate epithelium provides protection against oxidative damage and, thereby, suppression of prostate cancer. Thus, our findings provide a molecular link between a gene whose inactivation is known to be involved in prostate carcinogenesis, namely Nkx3.1, and oxidative damage of the prostatic epithelium.
- Published
- 2005
- Full Text
- View/download PDF
24. Composition and histone substrates of polycomb repressive group complexes change during cellular differentiation.
- Author
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Kuzmichev A, Margueron R, Vaquero A, Preissner TS, Scher M, Kirmizis A, Ouyang X, Brockdorff N, Abate-Shen C, Farnham P, and Reinberg D
- Subjects
- Animals, DNA-Binding Proteins, Enhancer of Zeste Homolog 2 Protein, Gene Expression Profiling, HeLa Cells, Histone-Lysine N-Methyltransferase, Humans, Macromolecular Substances, Male, Mice, Multigene Family, Oligonucleotide Array Sequence Analysis, Polycomb Repressive Complex 2, Polycomb-Group Proteins, Prostatic Neoplasms genetics, Prostatic Neoplasms metabolism, Prostatic Neoplasms pathology, Protein Isoforms genetics, Protein Isoforms metabolism, Proteins genetics, Proteins metabolism, RNA Interference, Sirtuin 1, Sirtuins genetics, Sirtuins metabolism, Substrate Specificity, Transcription Factors, Cell Differentiation physiology, Gene Expression Regulation, Histones metabolism, Repressor Proteins genetics, Repressor Proteins metabolism
- Abstract
Changes in the substrate specificities of factors that irreversibly modify the histone components of chromatin are expected to have a profound effect on gene expression through epigenetics. Ezh2 is a histone-lysine methyltransferase with activity dependent on its association with other components of the Polycomb Repressive Complexes 2 and 3 (PRC2/3). Ezh2 levels are increasingly elevated during prostate cancer progression. Other PRC2/3 components also are elevated in cancer cells. Overexpression of Ezh2 in tissue culture promotes formation of a previously undescribed PRC complex, PRC4, that contains the NAD+-dependent histone deacetylase SirT1 and isoform 2 of the PRC component Eed. Eed2 is expressed in cancer and undifferentiated embryonic stem (ES) cells but is undetectable in normal and differentiated ES cells. The distinct PRCs exhibit differential histone substrate specificities. These findings suggest that formation of a transformation-specific PRC complex may have a major role in resetting patterns of gene expression by regulating chromatin structure.
- Published
- 2005
- Full Text
- View/download PDF
25. A critical role for p27kip1 gene dosage in a mouse model of prostate carcinogenesis.
- Author
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Gao H, Ouyang X, Banach-Petrosky W, Borowsky AD, Lin Y, Kim M, Lee H, Shih WJ, Cardiff RD, Shen MM, and Abate-Shen C
- Subjects
- Animals, Cyclin D1 genetics, Cyclin-Dependent Kinase Inhibitor p27, Disease Models, Animal, Disease Progression, Gene Expression Regulation, Neoplastic, Genotype, Homeodomain Proteins, Male, Mice, Mice, Knockout, Mice, Mutant Strains, PTEN Phosphohydrolase, Prostatic Neoplasms etiology, Protein Tyrosine Phosphatases deficiency, Transcription Factors deficiency, Tumor Suppressor Proteins deficiency, Cell Cycle Proteins genetics, Gene Dosage, Prostatic Neoplasms genetics, Tumor Suppressor Proteins genetics
- Abstract
In human prostate cancer, the frequent down-regulation of p27(kip1) protein expression is correlated with poor clinical outcome, yet p27(kip1) rarely undergoes mutational inactivation. Here, we investigate the consequences of reducing or eliminating p27(kip1) function for prostate carcinogenesis in the context of a mouse modeling lacking the Nkx3.1 homeobox gene and the Pten tumor suppressor. Unexpectedly, we find that triple mutant mice heterozygous for a p27(kip1) null allele (Nkx3.1(+/- or -/-); Pten(+/-); p27(+/-)) display enhanced prostate carcinogenesis, whereas mice that are homozygous null for p27(kip1) (Nkx3.1(+/- or -/-); Pten(+/-); p27(-/-)) show inhibition of cancer progression. Expression profiling reveals that Cyclin D1 is highly up-regulated in compound p27(kip1) heterozygotes, but is down-regulated in the compound p27(kip1) homozygous mutants. Using RNA interference in prostate cancer cell lines with distinct p27(kip1) gene doses, we show that prostate tumorigenicity depends on levels of p27(kip1) and that the consequences of p27(kip1) gene dosage can be attributed, in part, to altered levels of Cyclin D1. Our findings suggest that p27(kip1) possesses dosage-sensitive positive as well as negative modulatory roles in prostate cancer progression.
- Published
- 2004
- Full Text
- View/download PDF
26. Inhibition of telomerase in tumor cells by ribozyme targeting telomerase RNA component.
- Author
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Liu B, Qu Y, Liu S, and Ouyang X
- Abstract
Telomerase plays an important role in cell proliferation and carcinogenesis and is believed to be a good target for anti-cancer drugs. Elimination of template function of telomerase RNA may repress the telomerase activity. A hammer-headed ribozyme (telomerase ribozyme, teloRZ) directed against the RNA component of human telomerase (hTR) was designed and synthesized. TeloRZ showed a specific cleavage activity against the hTR. The cleavage efficacy reached 60%. A eukaryotic expression plasmid containing teloRZ gene was inducted into HeLa cells by lipofectamine, the telomerase activity in HeLa cells expressing teloRZ decreased to one eighth of that in the control cells. The doubling time increased significantly and the apoptosis ratio was elevated with increasing population doublings (PDS). After 19-20 PDS 95% cells were apoptotic. To further investigate the effect of teloRZ on tumor growth, the eukaryotic expression plasmid containing teloRZ was injected into transplanted tumor of nude mouse. The teloRZ effectively inhibited the telomerase activity in transplanted tumor, promoted apoptosis of the transplanted tumor cells, and decreased the tumor size significantly. These results indicate that teloRZ can effectively inhibit telomerase activity and growth of tumor cells, and suggest the potential use of this ribozyme in anti-cancer therapy.
- Published
- 2002
- Full Text
- View/download PDF
27. [The relationship of p53 gene mutation to cell differentiation and metastasis of laryngeal squamous cell carcinoma].
- Author
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Zhang X, Wang L, Liu S, Ouyang X, and Liang C
- Subjects
- Carcinoma, Squamous Cell secondary, Exons, Humans, Laryngeal Neoplasms pathology, Lymphatic Metastasis genetics, Neoplasm Staging, Polymerase Chain Reaction methods, Polymorphism, Single-Stranded Conformational, Sequence Analysis, DNA methods, Carcinoma, Squamous Cell genetics, Cell Differentiation genetics, Laryngeal Neoplasms genetics, Mutation, Tumor Suppressor Protein p53 genetics
- Abstract
Objective: To inquire about the relationship of p53 gene mutation to the histopathological findings and clinical manifestation in cases of laryngeal squamous cell carcinoma(LSCC)., Methods: The fresh samples from 60 cases of LSCC were examined. Polymerase chain reaction and silver staining-single strand conformation polymorphism (PCR-SSCP) and DNA direct sequencing were used to detect the mutation of p53 gene in exons 5-8., Results: The mutation rates were 69.2% and 85.3% in patients at clinical stage I-II and stage III-IV respectively (P>0.05). In the well-, moderately- and poorly-differentiated cell of LSCC, the mutation rates were 52.9%, 83.3% and 94.7% respectively (P<0.05). The p53 gene mutation rate of LSCC patients with neck lymph-node metastasis was 96.4%, whereas that of patients without neck lymph-node metastasis was 62.5% (P<0.05). Twenty samples showed positive results in SSCP; 19 samples showed deletion and mutation in codons 125-292 by DNA direct sequencing., Conclusion: The mutation of p53 gene in exons 5-8 was closely related to cell differentiation and the neck lymph-node metastasis of LSCC, but it was not related to the clinical stages of the LSCC cases.
- Published
- 2002
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