12 results on '"P, Bare"'
Search Results
2. TNF-α Levels in Respiratory Samples Are Associated with SARS-CoV-2 Infection.
- Author
-
Pereson MJ, Badano MN, Aloisi N, Chuit R, E de Bracco MM, and Bare P
- Subjects
- Adolescent, Adult, Aged, Aged, 80 and over, Child, Female, Genome, Viral, Humans, Interleukin-6 analysis, Male, Middle Aged, Respiratory System chemistry, Respiratory System virology, Young Adult, COVID-19 immunology, SARS-CoV-2 genetics, SARS-CoV-2 immunology, Tumor Necrosis Factor-alpha analysis
- Published
- 2022
- Full Text
- View/download PDF
3. The Burden of and Risk Factors for Trachoma in Selected Districts of Zimbabwe: Results of 16 Population-Based Prevalence Surveys.
- Author
-
Phiri I, Manangazira P, Macleod CK, Mduluza T, Dhobbie T, Chaora SG, Chigwena C, Katiyo J, Willis R, Bakhtiari A, Bare P, Courtright P, Macheka B, Midzi N, and Solomon AAW
- Subjects
- Adolescent, Adult, Aged, Child, Child, Preschool, Cost of Illness, Cross-Sectional Studies, Female, Humans, Infant, Male, Middle Aged, Prevalence, Risk Factors, Trichiasis epidemiology, Young Adult, Zimbabwe epidemiology, Trachoma epidemiology
- Abstract
Background: Trachoma, a leading cause of blindness, is targeted for global elimination as a public health problem by 2020. In order to contribute to this goal, countries should demonstrate reduction of disease prevalence below specified thresholds, after implementation of the SAFE strategy in areas with defined endemicity. Zimbabwe had not yet generated data on trachoma endemicity and no specific interventions against trachoma have yet been implemented., Methods: Two trachoma mapping phases were successively implemented in Zimbabwe, with eight districts included in each phase, in September 2014 and October 2015. The methodology of the Global Trachoma Mapping Project was used., Results: Our teams examined 53,211 people for trachoma in 385 sampled clusters. Of 18,196 children aged 1-9 years examined, 1526 (8.4%) had trachomatous inflammation-follicular (TF). Trichiasis was observed in 299 (1.0%) of 29,519 people aged ≥15 years. Of the 16 districts surveyed, 11 (69%) had TF prevalences ≥10% in 1-9-year-olds, indicative of active trachoma being a significant public health problem, requiring implementation of the A, F and E components of the SAFE strategy for at least 3 years. The total estimated trichiasis backlog across the 16 districts was 5506 people. The highest estimated trichiasis burdens were in Binga district (1211 people) and Gokwe North (854 people)., Conclusion: Implementation of the SAFE strategy is needed in parts of Zimbabwe. In addition, Zimbabwe needs to conduct more baseline trachoma mapping in districts adjacent to those identified here as having a public health problem from the disease.
- Published
- 2018
- Full Text
- View/download PDF
4. Preferential association of hepatitis C virus with CD19 + B cells is mediated by complement system.
- Author
-
Wang RY, Bare P, De Giorgi V, Matsuura K, Salam KA, Grandinetti T, Schechterly C, and Alter HJ
- Subjects
- Cells, Cultured, Humans, Leukocytes, Mononuclear immunology, Leukocytes, Mononuclear virology, Antigens, CD19, B-Lymphocytes immunology, B-Lymphocytes virology, Hepacivirus physiology, Hepatitis C, Chronic immunology, Hepatitis C, Chronic virology, Receptors, Complement immunology
- Abstract
Extrahepatic disease manifestations are common in chronic hepatitis C virus (HCV) infection. The mechanism of HCV-related lymphoproliferative disorders is not fully understood. Recent studies have found that HCV in peripheral blood mononuclear cells from chronically infected patients is mainly associated with cluster of differentiation 19-positive (CD19
+ ) B cells. To further elucidate this preferential association of HCV with B cells, we used in vitro cultured virus and uninfected peripheral blood mononuclear cells from healthy blood donors to investigate the necessary serum components that activate the binding of HCV to B cells. First, we found that the active serum components were present not only in HCV carriers but also in HCV recovered patients and HCV-negative, healthy blood donors and that the serum components were heat-labile. Second, the preferential binding activity of HCV to B cells could be blocked by anti-complement C3 antibodies. In experiments with complement-depleted serum and purified complement proteins, we demonstrated that complement proteins C1, C2, and C3 were required to activate such binding activity. Complement protein C4 was partially involved in this process. Third, using antibodies against cell surface markers, we showed that the binding complex mainly involved CD21 (complement receptor 2), CD19, CD20, and CD81; CD35 (complement receptor 1) was involved but had lower binding activity. Fourth, both anti-CD21 and anti-CD35 antibodies could block the binding of patient-derived HCV to B cells. Fifth, complement also mediated HCV binding to Raji cells, a cultured B-cell line derived from Burkitt's lymphoma., Conclusion: In chronic HCV infection, the preferential association of HCV with B cells is mediated by the complement system, mainly through complement receptor 2 (CD21), in conjunction with the CD19 and CD81 complex. (Hepatology 2016;64:1900-1910)., Competing Interests: Potential conflict of interest: Nothing to report., (© 2016 by the American Association for the Study of Liver Diseases. This article has been contributed to by U.S. Government employees and their work is in the public domain in the USA.)- Published
- 2016
- Full Text
- View/download PDF
5. Hepatitis C virus diversification in Argentina: comparative analysis between the large city of Buenos Aires and the small rural town of O'Brien.
- Author
-
Golemba MD, Culasso AC, Villamil FG, Bare P, Gadano A, Ridruejo E, Martinez A, Di Lello FA, and Campos RH
- Subjects
- Aged, Argentina epidemiology, Bayes Theorem, Female, Genotype, Hepacivirus genetics, Hepacivirus physiology, Hepatitis C epidemiology, Hepatitis C virology, Humans, Male, Middle Aged, Phylogeny, Prevalence, Biodiversity, Cities epidemiology, Hepacivirus classification, Hepacivirus isolation & purification, Rural Population statistics & numerical data
- Abstract
Background: The estimated prevalence of HCV infection in Argentina is around 2%. However, higher rates of infection have been described in population studies of small urban and rural communities. The aim of this work was to compare the origin and diversification of HCV-1b in samples from two different epidemiological scenarios: Buenos Aires, a large cosmopolitan city, and O'Brien, a small rural town with a high prevalence of HCV infection., Patients and Methods: The E1/E2 and NS5B regions of the viral genome from 83 patients infected with HCV-1b were sequenced. Phylogenetic analysis and Bayesian Coalescent methods were used to study the origin and diversification of HCV-1b in both patient populations., Results: Samples from Buenos Aires showed a polyphyletic behavior with a tMRCA around 1887-1900 and a time of spread of infection approximately 60 years ago. In contrast, samples from ÓBrien showed a monophyletic behavior with a tMRCA around 1950-1960 and a time of spread of infection more recent than in Buenos Aires, around 20-30 years ago., Conclusion: Phylogenetic and coalescence analysis revealed a different behavior in the epidemiological histories of Buenos Aires and ÓBrien. HCV infection in Buenos Aires shows a polyphyletic behavior and an exponential growth in two phases, whereas that in O'Brien shows a monophyletic cluster and an exponential growth in one single step with a more recent tMRCA. The polyphyletic origin and the probability of encountering susceptible individuals in a large cosmopolitan city like Buenos Aires are in agreement with a longer period of expansion. In contrast, in less populated areas such as O'Brien, the chances of HCV transmission are strongly restricted. Furthermore, the monophyletic character and the most recent time of emergence suggest that different HCV-1b ancestors (variants) that were in expansion in Buenos Aires had the opportunity to colonize and expand in O'Brien.
- Published
- 2013
- Full Text
- View/download PDF
6. Investigation of residual hepatitis C virus in presumed recovered subjects.
- Author
-
Fujiwara K, Allison RD, Wang RY, Bare P, Matsuura K, Schechterly C, Murthy K, Marincola FM, and Alter HJ
- Subjects
- Adult, Aged, Animals, B-Lymphocytes virology, Carrier State virology, Disease Reservoirs virology, Female, Humans, Male, Middle Aged, Pan troglodytes virology, Viral Load, Hepacivirus genetics, Hepatitis C, Chronic virology, Leukocytes, Mononuclear virology, RNA, Viral blood
- Abstract
Unlabelled: Recent studies have found hepatitis C virus (HCV) RNA in peripheral blood mononuclear cells (PBMCs) of the majority of presumed recovered subjects. We investigated this unexpected finding using samples from patients whose HCV RNA and anti-HCV status had been serially confirmed. HCV RNA was detected in PBMCs from 66 of 67 chronic HCV carriers. Subpopulation analysis revealed that the viral load (log copies/10(6) cells) in B cells (4.14 ± 0.71) was higher than in total PBMCs (3.62 ± 0.71; P < 0.05), T cells (1.67 ± 0.88; P < 0.05), and non-B/T cells (2.48 ± 1.15; P < 0.05). HCV negative-strand RNA was not detected in PBMCs from any of 25 chronically infected patients. No residual viral RNA was detected in total PBMCs or plasma of 59 presumed recovered subjects (11 spontaneous and 48 treatment induced) using nested real-time polymerase chain reaction with a detection limit of 2 copies/μg RNA (from ≈ 1 × 10(6) cells). PBMCs from 2 healthy HCV-negative blood donors became HCV RNA positive, with B-cell predominance, when mixed in vitro with HCV RNA-positive plasma, thus passively mimicking cells from chronic HCV carriers. No residual HCV was detected in liver or other tissues from 2 spontaneously recovered chimpanzees., Conclusion: (1) HCV RNA was detected in PBMCs of most chronic HCV carriers and was predominant in the B-cell subpopulation; (2) HCV detected in PBMCs was in a nonreplicative form; (3) HCV passively adsorbed to PBMCs of healthy controls in vitro, becoming indistinguishable from PBMCs of chronic HCV carriers; and (4) residual HCV was not detected in plasma or PBMCs of any spontaneous or treatment-recovered subjects or in chimpanzee liver, suggesting that the classic pattern of recovery from HCV infection is generally equivalent to viral eradication., (Copyright © 2012 American Association for the Study of Liver Diseases.)
- Published
- 2013
- Full Text
- View/download PDF
7. [AIDS related lymphomas: Histopathological subtypes and association with Epstein Barr virus and Human Herpes virus type-8].
- Author
-
Corti M, de Dios Soler M, Bare P, Villafañe MF, De Tezanos Pinto M, Perez Bianco R, and Narbaitz M
- Subjects
- Adult, Female, Hodgkin Disease pathology, Humans, Immunohistochemistry, In Situ Hybridization, Lymphoma, AIDS-Related classification, Lymphoma, AIDS-Related pathology, Lymphoma, Non-Hodgkin pathology, Male, Middle Aged, Risk Factors, DNA, Viral analysis, Herpesvirus 4, Human genetics, Hodgkin Disease virology, Lymphoma, AIDS-Related virology, Lymphoma, Non-Hodgkin virology
- Abstract
Non-Hodgkin lymphomas (NHL) of the B-cell type are the second most common neoplasm among patients with human immunodeficiency virus (HIV) infection and AIDS. Here, we evaluated 48 cases of AIDS-related lymphomas (ARL) diagnosed at the Histopathological Division of the Instituto de Investigaciones Hematológicas of the National Academy of Medicine. Five were females and 43 were males with a median of age of 37 years at the time of the diagnosis. Micrometer sections were prepared and stained with hematoxilin-eosin; immunohistochemical examination for the presence of Epstein-Barr virus (EBV) was carried out in 48/48 cases. Additionally, biotinilated oligonucleotides were used to determine the presence of DNA of the Human Herpes virus type-8 (HHV-8) in 14/14 biopsy smears corresponding to plasmablastic lymphomas (PL). All were fenotype B cell lymphomas with an aggressive course and advanced neoplasm disease at the time of diagnosis. Virological findings showed the strong association between EBV and AIDS-related NHL. According to the histopathological subtype, the EBV genome was detected in 16/21 (76%) diffuse large B cell lymphomas, 1/3 Burkitt lymphoma and 3/4 (75%) of primary central nervous system lymphomas. Globally, EBV genome was detected in 20/28 NHL of this series. Detection of HHV-8 was negative in all cases of PL. Hodgkin lymphoma were more frequent in males 18/20 (90%), with an aggressive clinical course and a significant predominance of the subtypes associated with worse prognosis (90% of cases). We detected a significant association between EBV and HL (90% of cases). We consider that all cases of AIDS related lymphomas should be assessed for the presence of EBV because its presence may play a role in the prognosis.
- Published
- 2010
8. [The role of dendritic cells in the infection by HIV and HCV].
- Author
-
Belmonte L, Parodi C, Bare P, Baston M, Bracco MM, and Ruibal-Ares B
- Subjects
- Cell Differentiation, Cell Lineage immunology, Cytokines immunology, Dendritic Cells cytology, HIV immunology, Hepacivirus immunology, Humans, Immunity, Cellular, T-Lymphocytes immunology, Toll-Like Receptors immunology, Virus Attachment, Virus Internalization, Dendritic Cells immunology, HIV Infections immunology, Hepatitis C, Chronic immunology
- Abstract
Dendritic cells are most important as antigen presenting cells during the induction of an effective immune response. Therefore, it is important to study their role during the generation of persistent or chronic viral infections, such as HIV or HCV infection. In this review we shall describe the phenotypic and functional characteristics of the different classes of dendritic cells and of their membrane receptors. Their participation in defence or facilitation mechanisms involved in the immune response against these viruses will be discussed. It is important to take this knowledge into account when trying to design therapeutic strategies for protection or reconstruction of the immune system that may be altered as a consequence of infection with HIV or HCV.
- Published
- 2007
9. Spontaneous HIV-1 replication in a B-lymphoblastoid cell line obtained from an HIV-1-positive patient with undetectable plasma viral load.
- Author
-
Belmonte L, Parodi C, Bare P, Corti M, Sanjuan N, de Bracco MM, and Ruibal-Ares BH
- Subjects
- Cell Line, Tumor, Humans, Viral Load, Virus Replication, B-Lymphocytes virology, Disease Reservoirs virology, HIV Infections immunology, HIV-1 physiology
- Published
- 2006
- Full Text
- View/download PDF
10. Nonhepatosplenic gamma delta T-cell lymphoma with initial testicular compromise.
- Author
-
Scolnik MP, Burgos RA, Paz A, Weinreiter M, del Carmen Ardaiz M, Bare P, Hanza MC, de Dios Soler MA, Narbaitz MI, Palacios MF, Sasot A, Huberman A, and de Bracco MM
- Subjects
- Humans, Male, Maxillary Sinus Neoplasms pathology, Middle Aged, Receptors, Antigen, T-Cell, gamma-delta analysis, Liver Neoplasms pathology, Lymphoma, T-Cell pathology, Splenic Neoplasms pathology, Testicular Neoplasms pathology
- Abstract
We report here a case of nonhepatosplenic gammadelta T-cell lymphoma with undescribed initial localization in testis, without hepatosplenomegaly or adenopathies, and subsequent development in the maxillary sinus. The maxillar mass biopsy revealed a T-cell infiltration, and its immunologic characterization by flow cytometry showed a gammadelta T-cell phenotype (CD45+, CD3+, CD2+, TCR gammadelta+), without expression of CD7, CD5, CD1a, TdT, CD4, CD8, TCR alphabeta, or NK antigens (CD16, CD56, and CD57). Clonal gamma-chain gene rearrangement by polymerase chain reaction (PCR) was detected in testicular and maxillar biopsies. Epstein-Barr virus type 1 (EBV) sequences were detected by molecular biology in the biopsy material, suggesting that this oncogenic virus may play a role in the genesis of the clonal expansion of gammadelta T-cells. The patient was initially treated with standard chemotherapeutic protocols, with poor response and aggressive course.
- Published
- 2000
- Full Text
- View/download PDF
11. HTLV-Is in Argentina are phylogenetically similar to those of other South American countries, but different from HTLV-Is in Africa.
- Author
-
Yamashita M, Picchio G, Veronesi R, Ohkura S, Bare P, and Hayami M
- Subjects
- Africa, Argentina, Human T-lymphotropic virus 1 genetics, Humans, Phylogeny, Repetitive Sequences, Nucleic Acid, South America, Human T-lymphotropic virus 1 classification
- Abstract
To understand the origin and past dissemination of human T-cell leukemia/lymphotropic virus type I (HTLV-I) in Latin America, we conducted a phylogenetic study of five new HTLV-I isolates from Argentina. We sequenced partial fragments of long terminal repeats (LTR) of the new HTLV-Is, and then the sequences were subjected to a phylogenetic analysis for comparison with other HTLV-Is of various geographical origins. Our results indicated that all the isolates were members of the Cosmopolitan group. Furthermore, most (four out of five isolates) of the new HTLV-Is belonged to the Transcontinental (A) subgroup, the most widespread subgroup of the four subgroups in the Cosmopolitan group. In this subgroup, they were closely related to HTLV-Is found in other South American countries including those of Amerindians, and were different from those found in Africa. In contrast, the remaining one HTLV-I (ARGMF) did not show any clear similarity to known HTLV-I isolates belonging to the Cosmopolitan group. The close similarity of South American HTLV-Is strongly suggests a common origin of the virus in this continent. Our results do not support the proposed idea of recent introduction of HTLV-I into South America as a consequence of the slave trade from Africa, where phylogenetically different HTLV-Is predominate.
- Published
- 1998
- Full Text
- View/download PDF
12. HTLV-I/II indeterminate serology and natural killer cell expansion.
- Author
-
Picchio GR, Bare P, Savignano R, Perez-Bianco R, Yamashita M, and Hayami M
- Subjects
- Adult, Agglutination Tests, HTLV-I Infections diagnosis, HTLV-II Infections diagnosis, Humans, Lymphocyte Count, Male, HTLV-I Antibodies blood, HTLV-I Infections immunology, HTLV-II Antibodies blood, HTLV-II Infections immunology, Killer Cells, Natural cytology
- Published
- 1996
- Full Text
- View/download PDF
Catalog
Discovery Service for Jio Institute Digital Library
For full access to our library's resources, please sign in.