1. Development of slide ELISA (SELISA) for detection of four poultry viral pathogens by direct heat fixation of viruses on glass slides.
- Author
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Desingu PA, Singh SD, Dhama K, Kumar OR, Singh R, and Singh RK
- Subjects
- Animals, Enzyme-Linked Immunosorbent Assay methods, Glass, Hot Temperature, Microscopy, Poultry, Virology methods, Virus Diseases diagnosis, Virus Diseases virology, DNA Viruses isolation & purification, Poultry Diseases diagnosis, Poultry Diseases virology, RNA Viruses isolation & purification, Specimen Handling methods, Veterinary Medicine methods, Virus Diseases veterinary
- Abstract
The development of an easy and simpler method of slide enzyme-linked immunosorbent assay (SELISA) for the diagnosis of four economically important poultry viruses viz., Newcastle disease virus (NDV), infectious bronchitis virus (IBV), infectious bursal disease virus (IBDV) and egg drop syndrome 76 virus (EDS 76) and the use of SELISA for semi quantitation of NDV are described. The positive signals for viral aggregates were detected under light microscope. This is the first report regarding the development of SELISA based on heat fixation for the diagnosis of viral pathogens., (Copyright © 2014 Elsevier B.V. All rights reserved.)
- Published
- 2014
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