1. In vitro antioxidant and inhibitory activity of water decoctions of carob tree (Ceratonia siliqua L.) on cholinesterases, α-amylase and α-glucosidase.
- Author
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Custódio L, Patarra J, Alberício F, Neng NR, Nogueira JM, and Romano A
- Subjects
- Antioxidants isolation & purification, Cholinesterases, Enzyme Inhibitors isolation & purification, Galactans chemistry, Gallic Acid chemistry, Gallic Acid isolation & purification, Gentisates chemistry, Gentisates isolation & purification, Mannans chemistry, Phenols chemistry, Phenols isolation & purification, Plant Bark chemistry, Plant Gums chemistry, Plant Leaves chemistry, Trees chemistry, alpha-Amylases antagonists & inhibitors, alpha-Glucosidases, Antioxidants chemistry, Enzyme Inhibitors chemistry, Fabaceae chemistry, Plant Extracts chemistry
- Abstract
This work reports the in vitro inhibitory activity of water decoctions of leaves, germ flour, pulp, locust bean gum and stem bark of carob tree on α-amylase, α-glucosidase, acetylcholinesterase and butyrylcholinesterase. The antioxidant activity and the chemical characterisation of the extracts made by spectrophotometric assays and by high-performance liquid chromatography are also reported. Leaves and stem bark decoctions strongly inhibited all the enzymes tested, had significant antioxidant activity and the highest total phenolics content. The major compounds were identified as gallic acid in the leaves and gentisic acid in the stem bark.
- Published
- 2015
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