1. Phytochemical Analysis, Biological Activities, and Docking of Phenolics from Shoot Cultures of Hypericum perforatum L. Transformed by Agrobacterium rhizogenes .
- Author
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Tusevski O, Todorovska M, Petreska Stanoeva J, and Gadzovska Simic S
- Subjects
- Plant Extracts chemistry, Plant Extracts pharmacology, Enzyme Inhibitors pharmacology, Enzyme Inhibitors chemistry, Plants, Genetically Modified, alpha-Amylases metabolism, alpha-Amylases antagonists & inhibitors, alpha-Glucosidases metabolism, alpha-Glucosidases chemistry, Hypericum chemistry, Hypericum metabolism, Molecular Docking Simulation, Phenols chemistry, Phenols pharmacology, Phenols metabolism, Plant Shoots chemistry, Plant Shoots metabolism, Agrobacterium, Phytochemicals chemistry, Phytochemicals pharmacology
- Abstract
Hypericum perforatum transformed shoot lines (TSL) regenerated from corresponding hairy roots and non-transformed shoots (NTS) were comparatively evaluated for their phenolic compound contents and in vitro inhibitory capacity against target enzymes (monoamine oxidase-A, cholinesterases, tyrosinase, α-amylase, α-glucosidase, lipase, and cholesterol esterase). Molecular docking was conducted to assess the contribution of dominant phenolic compounds to the enzyme-inhibitory properties of TSL samples. The TSL extracts represent a rich source of chlorogenic acid, epicatechin and procyanidins, quercetin aglycone and glycosides, anthocyanins, naphthodianthrones, acyl-phloroglucinols, and xanthones. Concerning in vitro bioactivity assays, TSL displayed significantly higher acetylcholinesterase, tyrosinase, α-amylase, pancreatic lipase, and cholesterol esterase inhibitory properties compared to NTS, implying their neuroprotective, antidiabetic, and antiobesity potential. The docking data revealed that pseudohypericin, hyperforin, cadensin G, epicatechin, and chlorogenic acid are superior inhibitors of selected enzymes, exhibiting the lowest binding energy of ligand-receptor complexes. Present data indicate that H. perforatum transformed shoots might be recognized as an excellent biotechnological system for producing phenolic compounds with multiple health benefits.
- Published
- 2024
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