1. Rutin and Its Cyclodextrin Inclusion Complexes: Physico-chemical Evaluation and in vitro Activity on B164A5 Murine Melanoma Cell Line.
- Author
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Corina D, Florina B, Iulia P, Cristina D, Rita A, Alexandra P, Virgil P, Hancianu M, Daliana M, and Codruta S
- Subjects
- Animals, Apoptosis drug effects, Calorimetry, Differential Scanning, Cell Line, Tumor, Cell Proliferation drug effects, Mice, Powder Diffraction, X-Ray Diffraction, 2-Hydroxypropyl-beta-cyclodextrin chemistry, 2-Hydroxypropyl-beta-cyclodextrin pharmacology, Antineoplastic Agents chemistry, Antineoplastic Agents pharmacology, Antioxidants chemistry, Antioxidants pharmacology, Rutin chemistry, Rutin pharmacology, beta-Cyclodextrins chemistry, beta-Cyclodextrins pharmacology
- Abstract
Background: Recent studies have shown that rutin presents a heightened interest due to the plethora of biological activities. The major drawback for this phytocompound is the poor water solubility, a parameter that limits its bioavailability. The study aimed to prepare and assess the inclusion complexes of rutin with β-cyclodextrin (BCD) and hydroxypropyl-β-cyclodextrin (HPBCD), followed by the evaluation of the antioxidant, antiproliferative and pro-apoptotic activity against B164A5 murine melanoma cell line., Methods: Inclusion complexes were prepared by kneading method. Scanning electron microscopy, differential scanning calorimetry and X-ray powder diffraction were used in order to assess their formation. Antioxidant activity was determined by DPPH assay. Antiproliferative and pro-apoptotic effects where tested by MTT, respectively Annexin V-PI assays. Physico-chemical methods proved that incorporation took place., Results: Results show that rutin presents antioxidant activity, and complexation increases this property. After 72 h of incubation at the concentration of 100 µM rutin proved to be an active antiproliferative and pro-apoptotic compound against B164A5 cells., Conclusion: Incorporation in BCD and HBCD maintained the activity, thus representing an important finding and open path for in vivo experiments., (Copyright© Bentham Science Publishers; For any queries, please email at epub@benthamscience.org.)
- Published
- 2017
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