1. Anti-inflammatory and angiogenic activity of polysaccharide extract obtained from Tibetan kefir.
- Author
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Prado MR, Boller C, Zibetti RG, de Souza D, Pedroso LL, and Soccol CR
- Subjects
- Angiogenesis Inducing Agents isolation & purification, Angiogenesis Inducing Agents toxicity, Animals, Anti-Inflammatory Agents isolation & purification, Anti-Inflammatory Agents toxicity, Cell Survival drug effects, Chick Embryo, Chickens, Chlorocebus aethiops, Dose-Response Relationship, Drug, Glycoside Hydrolase Inhibitors isolation & purification, Glycoside Hydrolase Inhibitors toxicity, Hyaluronoglucosaminidase metabolism, Polysaccharides, Bacterial isolation & purification, Polysaccharides, Bacterial toxicity, Vero Cells, Angiogenesis Inducing Agents pharmacology, Anti-Inflammatory Agents pharmacology, Chorioallantoic Membrane blood supply, Glycoside Hydrolase Inhibitors pharmacology, Hyaluronoglucosaminidase antagonists & inhibitors, Kefir microbiology, Neovascularization, Physiologic drug effects, Polysaccharides, Bacterial pharmacology
- Abstract
The search for new bioactive molecules is a driving force for research pharmaceutical industries, especially those molecules obtained from fermentation. The molecules possessing angiogenic and anti-inflammatory attributes have attracted attention and are the focus of this study. Angiogenic activity from kefir polysaccharide extract, via chorioallantoic membrane assay, exhibited a pro-angiogenic effect compared with vascular endothelial factor (pro-angiogenic) and hydrocortisone (anti-angiogenic) activity as standards with an EC50 of 192ng/mL. In terms of anti-inflammatory activity determined via hyaluronidase enzyme assay, kefir polysaccharide extract inhibited the enzyme with a minimal activity of 2.08mg/mL and a maximum activity of 2.57mg/mL. For pharmaceutical purposes, kefir polysaccharide extract is considered to be safe because it does not inhibit VERO cells in cytotoxicity assays., (Copyright © 2016 Elsevier Inc. All rights reserved.)
- Published
- 2016
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