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Objective . Bragg peak measurements play a key role in the beam quality assurance in proton therapy. Used as base data for the treatment planning softwares, the accuracy of the data is crucial when defining the range of the protons in the patient. Approach . In this paper a protocol to reconstruct a Pristine Bragg Peak exploring the direct correlation between the particle flux and the dose deposited by particles is presented. Proton flux measurements at the HollandPTC and FLUKA Monte Carlo simulations are used for this purpose. This new protocol is applicable to plastic scintillator detectors developed for Quality Assurance applications. In order to obtain the Bragg curve using a plastic fiber detector, a PMMA phantom with a decoupled and moveable stepper was designed. The step phantom allows to change the depth of material in front of the fiber detector during irradiations. The Pristine Bragg Peak reconstruction protocol uses the measured flux of particles at each position and multiplies it by the average dose obtained from the Monte Carlo simulation at each position. Main results . The results show that with this protocol it is possible to reconstruct the Bragg Peak with an accuracy of about 470 µ m, which is in accordance with the tolerances set by the AAPM. Significance . It has the advantage to be able to overcome the quenching problem of scintillators in the high ionization density region of the Bragg peak., (© 2024 Institute of Physics and Engineering in Medicine. All rights, including for text and data mining, AI training, and similar technologies, are reserved.)

Background: Diet and exercise interventions have been associated with improved body composition and physical fitness. However, evidence regarding their combined effects in older adults is scarce. This study aimed to investigate the impact of a combined 12-week Mediterranean diet-based sustainable healthy diet (SHD) and multicomponent training (MT) intervention on body composition, anthropometry, and physical fitness in older adults., Methods: Diet intervention groups received a weekly SHD food supply and four sessions, including a SHD culinary practical workshop. The exercise program included MT 50 min group session, three times a week, on non-consecutive days. Body composition and physical fitness variables were assessed through dual X-ray absorptiometry, anthropometric measurements, and senior fitness tests. Repeated measures ANOVA, with terms for group, time, and interaction, was performed., Results: Our results showed that a combined intervention significantly lowered BMI and total fat. Also, significant differences between assessments in all physical fitness tests, except for aerobic endurance, were observed. Adjusted models show significant differences in BMI ( p = 0.049) and WHR ( p = 0.037) between groups and in total fat ( p = 0.030) for the interaction term. Body strength ( p < 0.001), balance tests ( p < 0.001), and aerobic endurance ( p = 0.005) had significant differences amongst groups. Considering the interaction term, differences were observed for upper body strength ( p = 0.046) and flexibility tests ( p = 0.004 sit and reach, p = 0.048 back scratch)., Conclusions: Our intervention study demonstrates the potential of implementing healthy lifestyle and sustainable models to promote healthy and active aging.

This study examined the effects of game schedule, travel demands and contextual factors on team game-related statistics during a full season. The top 10 teams competing in the 2020-2021 Euroleague basketball season were included where game-related statistics from their respective national competitions and the Euroleague competition were retrieved (761 games). Hierarchical linear regression models were computed to evaluate the effects of distance travelled, game schedule and contextual factors for the previous and current games (league, season phase, opponent level, game outcome, score differential) on key performance indicators (points, shooting, rebounds, assists, turnovers, fouls). Several significant models ( p  < 0.05) yielded R 2 values ranging from 0.05 to 0.22 with small -to- medium effect magnitudes. Analyses revealed significant associations between longer durations separating games and less free-throws being made and between further distances travelled and worse 3-point shooting, more offensive rebounds and more fouls. Regarding contextual factors, favourable outcomes for shooting, assists, steals, fouling and turnovers were significantly associated with team success. Playing higher-level teams and competing in playoffs or finals was associated with several diminished outcomes. These results emphasize the multi-factorial nature of performance in elite European basketball, with game schedule, travel and various contextual factors requiring consideration in developing holistic operational plans for teams.

Background and Aims: Healthy dietary patterns and exercise practices have been associated with improved metabolic and inflammatory profiles. However, studies regarding the combined effect of these interventions on plasma biomarkers and metabolome in older adults are sparser. The primary aim of this study was to investigate the impact of a combined Mediterranean Diet-based Sustainable Healthy Diet (SHD) and Multicomponent Training (MT) intervention on the plasma biomarkers and metabolome and how dietary intake and exercise could modulate these effects., Methods: SHD intervention included a weekly supply of Mediterranean Diet-based SHD food and four nutrition sessions involving a Mediterranean-Diet culinary workshop, and the exercise program included 50-min MT group sessions, held three times a week, lasting both 12 weeks. Plasma biomarkers were obtained through standard biochemical analysis. A proton ( 1 H) nuclear magnetic resonance (NMR) spectroscopy-based metabolomics approach was used to study the metabolome in blood plasma. Repeated measures ANOVA were performed and adjusted for confounders., Results: SHD + MT intervention significantly decreased HDL-C and calcium. SHD + MT showed some changes in common with the SHD and MT group, namely a significant decrease in citrate levels (p = 0.009 for SHD + MT; p = 0.037 for SHDT) and an increase in pyruvate (p < 0.001 for MT and SHD + MT). The SHD + MT group also revealed specific changes in the levels of some amino acids (decrease in alanine, glutamine and lysine: p = 0.026; p < 0.001; p = 0.038, respectively). Increases in formate (p = 0.025) and unsaturated lipids (p = 0.011) are consistent with changes in energy and lipoprotein metabolism., Conclusion: Our data show that a combined lifestyle intervention program, including a Mediterranean Diet-based SHD and MT, could modulate biomarker and metabolome and there seems to be a metabolic path associated to these interventions in older adults. Due to its wide-ranging relevance, it is pertinent to assess to what extent combined SHD and MT can contribute to better clinical profiles., Competing Interests: Conflict of interest The authors declare that they have no competing interests., (Copyright © 2024 The Authors. Published by Elsevier Ltd.. All rights reserved.)

The design of complex health systems interventions, such as pay for performance (P4P), can be critical to determining such programmes' success. In P4P programmes, the design of financial incentives is crucial in shaping how these programmes work. However, the design of such schemes is usually homogenous across providers within a given scheme. Consequently, there is a limited understanding of the strengths and weaknesses of P4P design elements from the implementers' perspective. This study takes advantage of the unique context of Brazil, where municipalities adapted the federal incentive design, resulting in variations in incentive design across municipalities. The study aims to understand why municipalities in Brazil chose certain P4P design features, the associated challenges and the local adaptations made to address problems in scheme design. This study was a multiple case study design relying on qualitative data from 20 municipalities from two states in Northeastern Brazil. We conducted two key informant interviews with municipal-level stakeholders and focus group discussions with primary care providers. We also reviewed municipal Primary Care Access and Quality laws in each municipality. We found substantial variation in the design choices made by municipalities regarding 'who was incentivized', the 'payment size' and 'frequency'. Design choices affected relationships within municipalities and within teams. Challenges were chiefly associated with fairness relating to 'who received the incentive', 'what is incentivized' and the 'incentive size'. Adaptations were made to improve fairness, mostly in response to pressure from the healthcare workers. The significant variation in design choices across municipalities and providers' response to them highlights the importance of considering local context in the design and implementation of P4P schemes and ensuring flexibility to accommodate local preferences and emerging needs. Attention is needed to ensure that the choice of 'who is incentivized' and the 'size of incentives' are inclusive and fair and the allocation and 'use of funds' are transparent., (© The Author(s) 2024. Published by Oxford University Press in association with The London School of Hygiene and Tropical Medicine.)

Introduction: This study aimed to describe the fixation location and the time of the longer fixation of expert and novice futsal coaches before the ball was in play in futsal set pieces., Methods: A total of 10 experts (ages 48 ± 5) and 10 novice coaches (ages 40 ± 7) participated in the study. They observed that 38 video clips were created to mimic the attack and defensive set-piece moments of the game. Data were collected in a standardized video analysis task using the pupil invisible eye tracker and processed through the pupil cloud platform. The Mann-Whitney test was conducted to evaluate differences in gaze duration between game moments (attack and defense set pieces) and groups (expert vs. novice). Gaze duration was also compared for gaze location between groups. For further comparisons, the game moments (attack and defense set pieces) and the gaze location were summarized in two-dimensional graphics using correspondence analysis., Results and Discussion: The results revealed higher values of gaze duration for attack and defense set pieces for the group of experts than for novices. When considering gaze duration, expert coaches had higher values than novices for the attacker 3, defender 3, barrier 1st, and barrier 2nd gaze locations. The correspondence analysis showed different strategies of visual search and, consequently, gaze locations for attack and defense set pieces. In particular, there was different correspondence for free kicks between the level of expertise and gaze location, while corner and sideline kicks revealed some correspondence between the groups and the gaze location. In free kicks, coaches should be particularly concerned about the relationship between attacker and defender three and the barrier 1st and 2nd line positions. In corner and sideline kicks, coaches should be particularly aware of the relationship between attackers' and defenders' positions., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. The author(s) declared that they were an editorial board member of Frontiers, at the time of submission. This had no impact on the peer review process and the final decision., (Copyright © 2024 Rodrigues, Leite, Ribeiro, Sampaio, Araújo and Travassos.)

Objectives: We performed a multicentre study (2020-2022) to compare the in vitro activity of ozenoxacin and comparator agents against Staphylococcus aureus and Streptococcus pyogenes clinical isolates from skin and soft-tissue infections (SSTI)., Methods: A total of 1725 isolates (1454 S. aureus and 271 S. pyogenes ) were collected in 10 centres from eight countries between January 2020 and December 2022. Antimicrobial susceptibility testing was determined (microdilution-SENSITITRE). Results were interpreted following European Committee on Antimicrobial Susceptibility Testing (EUCAST) 2023 (clinical breakpoints, ECOFF) and CLSI criteria., Results: Ozenoxacin exhibited high in vitro activity against S. aureus (MIC 50/90  = 0.002/0.12 mg/L) and S. pyogenes (MIC 50/90  = 0.015/0.03 mg/L), inhibiting 99% of the isolates at MIC ≤ 0.5 mg/L and at MIC ≤ 0.06, respectively. The most active comparators against S. aureus were retapamulin (MIC 90  = 0.12 mg/L), fusidic acid (MIC 90  = 0.25 mg/L) and mupirocin (MIC 90  = 0.5 mg/L); and against S. pyogenes were retapamulin (MIC 90  = 0.03 mg/L), clindamycin (MIC 90  = 0.12 mg/L) and mupirocin (MIC 90  = 0.25 mg/L). Ciprofloxacin and methicillin resistant rates for S. aureus were 31.3% (455/1454) and 41% (598/1454), respectively. Additionally, 62% (373/598) of the MRSA were also ciprofloxacin non-susceptible, whereas only 10% (23/271) of the MSSA were ciprofloxacin resistant. Ozenoxacin was more active against ciprofloxacin-susceptible S. aureus than against ciprofloxacin-resistant isolates, and showed a slightly higher MIC in MRSA isolates than in MSSA. However, ozenoxacin activity was comparable in both ciprofloxacin-resistant MSSA and MRSA subsets. On the other hand, ozenoxacin had similar activity in ciprofloxacin-susceptible and resistant S. pyogenes isolates., Conclusions: Ozenoxacin is a potent antimicrobial agent of topic use against Gram-positive bacteria causing SSTI, including MRSA isolates non-susceptible to ciprofloxacin., (© The Author(s) 2024. Published by Oxford University Press on behalf of British Society for Antimicrobial Chemotherapy.)

Pay-for-performance (P4P) schemes have been shown to have mixed effects on health care outcomes. A challenge in interpreting this evidence is that P4P is often considered a homogenous intervention, when in practice schemes vary widely in their design. Our study contributes to this literature by providing a detailed depiction of incentive design across municipalities within a national P4P scheme in Brazil [Primary Care Access and Quality (PMAQ)] and exploring the association of alternative design typologies with the performance of primary health care providers. We carried out a nation-wide survey of municipal health managers to characterize the scheme design, based on the size of the bonus, the providers incentivized and the frequency of payment. Using OLS regressions and controlling for municipality characteristics, we examined whether each design feature was associated with better family health team (FHT) performance. To capture potential interactions between design features, we used cluster analysis to group municipalities into five design typologies and then examined associations with quality of care. A majority of the municipalities included in our study used some of the PMAQ funds to provide bonuses to FHT workers, while the remaining municipalities spent the funds in the traditional way using input-based budgets. Frequent bonus payments (monthly) and higher size bonus allocations (share of 20-80%) were strongly associated with better team performance, while who within a team was eligible to receive bonuses did not in isolation appear to influence performance. The cluster analysis showed what combinations of design features were associated with better performance. The PMAQ score in the 'large bonus/many workers/high-frequency' cluster was 8.44 points higher than the 'no bonus' cluster, equivalent to a difference of 21.7% in the mean PMAQ score. Evidence from our study shows how design features can potentially influence health provider performance, informing the design of more effective P4P schemes., (© The Author(s) 2024. Published by Oxford University Press in association with The London School of Hygiene and Tropical Medicine.)

Tools for accessing and studying organelles remain underdeveloped. Here, we present a method by which giant organelle vesicles (GOVs) are generated by submitting cells to a hypotonic medium followed by plasma membrane breakage. By this means, GOVs ranging from 3 to over 10 µm become available for micromanipulation. GOVs are made from organelles such as the endoplasmic reticulum, endosomes, lysosomes and mitochondria, or in contact with one another such as giant mitochondria-associated ER membrane vesicles. We measure the mechanical properties of each organelle-derived GOV and find that they have distinct properties. In GOVs procured from Cos7 cells, for example, bending rigidities tend to increase from the endoplasmic reticulum to the plasma membrane. We also found that the mechanical properties of giant endoplasmic reticulum vesicles (GERVs) vary depending on their interactions with other organelles or the metabolic state of the cell. Lastly, we demonstrate GERVs' biochemical activity through their capacity to synthesize triglycerides and assemble lipid droplets. These findings underscore the potential of GOVs as valuable tools for studying the biophysics and biology of organelles., (© 2024. The Author(s).)

Two isolates representing a novel species of the genus Wickerhamiella were obtained in India from nectar of flowers of Lantana camara , an ornamental exotic species native to Central and South America. Phylogenetic analyses of the D1/D2 domain of the 26S large subunit (LSU) rRNA gene, internal transcribed spacer (ITS) region, and physiological characteristics, supported the recognition of the novel species, that we designate Wickerhamiella lachancei sp. nov (MycoBank no. MB851709), with MCC 9929 T as the holotype and PYCC 10003 T as the isotype. Considering pairwise sequence similarity, the type strain of the novel species differs from the type strain of the most closely related species, Wickerhamiella drosophilae CBS 8459 T , by 16 nucleotide substitutions and two gaps (3.9 % sequence variation) in the D1/D2 region (560 bp compared) and 28 nucleotide substitutions and five gaps (7.22 % sequence variation) in the ITS region (444 bp compared).

This study aimed to examine the most demanding passages of play in elite youth soccer for congested and non-congested fixture schedules. Seventeen elite youth male soccer players (18.2 ± 1.3 years old) participated in this study across 30 competitive matches. Assessed matches included congested (n = 12, three matches within eight consecutive days or less) and non-congested matches (n = 18, at least 5 days between matches). The players' activity profiles during matches were analysed using global positioning measurement units (GPS). Players activity included: distance covered, distance covered at different velocities, high-intensity accelerations and decelerations, and player load. The most demanding passages (MDP) of match play was calculated using a moving average method within three-time windows (i.e., 1, 5, and 10 min). Data were analysed using a Bayesian ANOVA. During congested fixtures, the players' distance covered and player load declined, with the former decreasing across all the MDP time windows, whereas the latter exclusively into the long-time windows (i.e., 5 and 10 min). Conversely, statistical differences in the remaining variables were anecdotal and in favour of the null hypothesis (i.e., Bayes factor <1), suggesting a non-influence of the competition fixture schedule. These findings provide insight into the MDP of youth soccer, helping practitioners to periodize training and recovery strategies during different competitive fixture schedules.

Background: Although youth soccer players meet and exceed the weekly physical activity (PA) guidelines also exhibit high off-training sedentary behaviour (SB) levels. Recent evidence indicated that low PA preceding training leads to decreased PA intensity in soccer practice and that SB impairs sports performance and recovery. Thus, a parallel group randomized trial examined the effect of activity wristbands on young players' off-training PA and SB profiles; assessed the added value of wearable wristbands with PA warnings; and investigated whether manipulating off-training PA can affect the players' training responses., Methods: Thirty-two adolescent soccer players (16.1 ± 0.9 years old) were monitored during weekdays for two weeks (interspersed with one week). Players were randomly assigned to a reminder to move (REM) and a non-reminder to move group (nREM). The REM wore an activity wristband (Fitbit Charge 2) with PA warnings in the last week of research, while the nREM wore identical monitors without PA feedback. Throughout the study, off-training PA was assessed using tri-axial accelerometers, and training responses were analysed using wearable inertial monitoring units. Gardner-Altman estimation plots and a Fisher's Exact Test of Independence estimated each group's off-training PA changes between the monitored weeks. Complementary, an analysis of covariance (ANCOVA) quantified the effect of Fitbit wristband configurations (with a reminder to move or without a reminder to move) on the off-training PA and soccer training external load variables. The independent variable group had two levels (REM and nREM). The off-training PA and training load values collected in the first week were introduced in the model as a covariate, the groups were designated fixed factors and the off-training PA and training load from the third week were the dependent variables. The team was also included as a covariate and the between-group differences post-intervention were adjusted for baseline and team., Results: Interestingly, results showed that different wearable wristbands did not influence the players' off-training PA profiles (p > 0.05). Concomitantly, no differences were observed in training responses (p > 0.05)., Conclusion: Findings emphasize the importance of health and youth sports organizations in developing newer approaches for promoting healthier lifestyles, beyond training practices, with potentially favourable implications for sports performance.

The current study aimed to investigate the effects of interchange rotations on players` physical performance during competition, with special reference to high-intensity activity (HIA) according to the playing position. Physical performance data, collected from 19 professional players during seven official matches from the Spanish futsal league using a portable local positioning system, included the number of high-speed running activities (>18 km·h -1 ), high-intensity accelerations (>3 m·s -2 ), and high-intensity decelerations (>3 m·s -2 ). Statistically significant (p ≤ 0.05) differences were observed in the number of HIA efforts across rotations and between positions. Players performed more HIA efforts in the first rotation (n = 17.6), suggesting that their first rotation was more demanding than all subsequent rotations. Wingers demonstrated a higher HIA effort and frequency of HIA efforts when compared to defenders (p ≤ 0.05) and pivots (p ≤ 0.001). For all positions, the first rotation was more physically demanding as the number of HIA efforts per rotation decreased with an increased number of rotations throughout the match. Furthermore, higher HIA profile positions, such as wingers and defenders, were less likely to maintain consistent HIA properties (repetition number, time-frequency, and the work-rate) across subsequent rotations during the match. The findings of the study can inform coaching decisions regarding players' rotations to maintain consistent HIA performance throughout the match., Competing Interests: The authors declare no conflict of interest., (Copyright: © Academy of Physical Education in Katowice.)

Abstract: Abade, E, Brito, J, Gonçalves, B, Saura, L, Coutinho, D, and Sampaio, J. Using deadlifts as a postactivation performance enhancement strategy in warm-ups in football. J Strength Cond Res 37(9): 1821-1827, 2023-Postactivation performance enhancement activities may be relevant warm-up strategies aiming to improve subsequent physical performance. The purpose of the current study was to investigate the effects of adding barbell deadlift or hex-bar deadlift exercises to current warm-up routines on running and jumping performances in football players. Ten highly trained male football players participated in the study during the competitive phase of the season. All players performed 3 protocols in the same week: a standard warm-up that included players' regular routines and 2 other protocols with the addition of barbell or hex-bar deadlift, after the end of the warm-up (3 sets of 3 reps, progressing set by set from 60% to 85% repetition maximum). All protocols had the same time interval between pretest (immediately after the warm-up) and posttest (15 minutes after the warm-up). Vertical jumping (countermovement jump [CMJ]; Abalakov jump [AJ]) and running performances (505 test) were impaired 15 minutes after the standard warm-up (CMJ: -6.7 ± 4.2%; AJ: -8.1 ± 8.4%; and 505 time: 1.4 ± 2.5%). For warm-up with the addition of barbell deadlift, vertical jump increased by 4.3 ± 5.6% (Cohen's dunb : 0.23 [0.02-0.47]) and 505 time decreased by -5.9 ± 3.6% (Cohen's dunb : 0.97 [-1.68 to -0.43]). The warm-up with hex-bar deadlift led to trivial changes for CMJ and AJ, but 505 time decrease by -2.7 ± 2.6% (Cohen's dunb : -0.53 [-1.01 to -0.13]). The deadlift exercise can be added to warm-up routines to maintain or even enhance acute physical performance. However, coaches and practitioners should be aware that performance enhancements resultant from deadlift may vary according to individual physical profiles., (Copyright © 2023 National Strength and Conditioning Association.)

Oxidation of PUFAs in LDLs trapped in the arterial intima plays a critical role in atherosclerosis. Though there have been many studies on the atherogenicity of oxidized derivatives of PUFA-esters of cholesterol, the effects of cholesteryl hemiesters (ChEs), the oxidation end products of these esters, have not been studied. Through lipidomics analyses, we identified and quantified two ChE types in the plasma of CVD patients and identified four ChE types in human endarterectomy specimens. Cholesteryl hemiazelate (ChA), the ChE of azelaic acid (n-nonane-1,9-dioic acid), was the most prevalent ChE identified in both cases. Importantly, human monocytes, monocyte-derived macrophages, and neutrophils exhibit inflammatory features when exposed to subtoxic concentrations of ChA in vitro. ChA increases the secretion of proinflammatory cytokines such as interleukin-1β and interleukin-6 and modulates the surface-marker profile of monocytes and monocyte-derived macrophage. In vivo, when zebrafish larvae were fed with a ChA-enriched diet, they exhibited neutrophil and macrophage accumulation in the vasculature in a caspase 1- and cathepsin B-dependent manner. ChA also triggered lipid accumulation at the bifurcation sites of the vasculature of the zebrafish larvae and negatively impacted their life expectancy. We conclude that ChA behaves as an endogenous damage-associated molecular pattern with inflammatory and proatherogenic properties., Competing Interests: Conflict of interest The authors declare that they have no conflicts of interest with the contents of this article., (Copyright © 2023 The Authors. Published by Elsevier Inc. All rights reserved.)

This study aimed to identify the effects of playing with additional individual (IND), collective (COL) or individual-collective (MIX) variability on youth football players' performance during small-sided games. Twelve youth football players (U17, age = 16.1 ± 0.9 years) played a goalkeeper (Gk) + 6 outfield players a-side (Gk+6vs6+Gk) under four conditions: (i) playing in the 1:2:3:1 formation without any other rule (control condition, CTR); (ii) 1:2:3:1 formation with additional body restrictions changing each minute (individual condition, IND); (iii) using different tactical formations modified each minute (collective condition, COL; (iv) using different tactical formations and body restrictions varied each minute (individual-collective condition, MIX). Generally, there were similar behaviours across conditions, especially for the CTR and the MIX. Nevertheless, the CTR condition presented moderate higher values in the lateral direction (p ≤ .05), while also higher longitudinal synchronization compared to the IND (p ≤ .05). The COL condition presented higher spatial exploration (p ≤ .05), which may justify the higher values for distance covered while running and sprinting (p ≤ .05). Overall, coaches may use the IND condition to refine players' technical actions, while the COL condition to develop players' ability to perform in different playing positions and team structures.

The aim of this study was to explore how situational variables affect youth soccer players' perceived exertion (RPE) after official matches. Thirty-five elite youth male players (14.33 ± 0.86 years; 173.49 ± 6.16 cm; 63.44 ± 5.98 kg) who belonged to two different teams of a professional club participated in this study. Data collection was conducted during two seasons (2016-2017, 2017-2018) and included 60 official matches (30 official matches per team). Ten minutes after each match players rated their RPE and using a modified Borg CR-10 scale. A Random Forest Regression was used to quantify the importance of match-related situational variables in RPE. Afterwards, a linear mixed model analysis was applied to identify the variability in RPE among the situational variables. The game-playing time, the player status (starter or substitute) and the player identity were the strongest predictors of RPE. Moreover, the match outcome and the final scoreline showed significant effects on both starter and substitute players but the main effect of the quality of the opponent was only identified in starter players (p < 0.05). These results allow practitioners to know how situational variables interact and modulate RPE after official matches and help them to prescribe and adapt the players' training content and load before and after matches., Competing Interests: Declaration of competing interest The authors report there are no competing interests to declare., (Copyright © 2023 Elsevier Ltd. All rights reserved.)

The subphylum Saccharomycotina is a lineage in the fungal phylum Ascomycota that exhibits levels of genomic diversity similar to those of plants and animals. The Saccharomycotina consist of more than 1 200 known species currently divided into 16 families, one order, and one class. Species in this subphylum are ecologically and metabolically diverse and include important opportunistic human pathogens, as well as species important in biotechnological applications. Many traits of biotechnological interest are found in closely related species and often restricted to single phylogenetic clades. However, the biotechnological potential of most yeast species remains unexplored. Although the subphylum Saccharomycotina has much higher rates of genome sequence evolution than its sister subphylum, Pezizomycotina , it contains only one class compared to the 16 classes in Pezizomycotina . The third subphylum of Ascomycota , the Taphrinomycotina , consists of six classes and has approximately 10 times fewer species than the Saccharomycotina . These data indicate that the current classification of all these yeasts into a single class and a single order is an underappreciation of their diversity. Our previous genome-scale phylogenetic analyses showed that the Saccharomycotina contains 12 major and robustly supported phylogenetic clades; seven of these are current families ( Lipomycetaceae, Trigonopsidaceae, Alloascoideaceae, Pichiaceae, Phaffomycetaceae, Saccharomycodaceae , and Saccharomycetaceae ), one comprises two current families ( Dipodascaceae and Trichomonascaceae ), one represents the genus Sporopachydermia , and three represent lineages that differ in their translation of the CUG codon (CUG-Ala, CUG-Ser1, and CUG-Ser2). Using these analyses in combination with relative evolutionary divergence and genome content analyses, we propose an updated classification for the Saccharomycotina , including seven classes and 12 orders that can be diagnosed by genome content. This updated classification is consistent with the high levels of genomic diversity within this subphylum and is necessary to make the higher rank classification of the Saccharomycotina more comparable to that of other fungi, as well as to communicate efficiently on lineages that are not yet formally named. Taxonomic novelties: New classes: Alloascoideomycetes M. Groenew., Hittinger, Opulente & A. Rokas, Dipodascomycetes M. Groenew., Hittinger, Opulente & A. Rokas, Lipomycetes M. Groenew., Hittinger, Opulente, A. Rokas, Pichiomycetes M. Groenew., Hittinger, Opulente & A. Rokas, Sporopachydermiomycetes M. Groenew., Hittinger, Opulente & A. Rokas, Trigonopsidomycetes M. Groenew., Hittinger, Opulente & A. Rokas. New orders: Alloascoideomycetes: Alloascoideales M. Groenew., Hittinger, Opulente & A. Rokas; Dipodascomycetes: Dipodascales M. Groenew., Hittinger, Opulente & A. Rokas; Lipomycetes: Lipomycetales M. Groenew., Hittinger, Opulente & A. Rokas; Pichiomycetes: Alaninales M. Groenew., Hittinger, Opulente & A. Rokas, Pichiales M. Groenew., Hittinger, Opulente & A. Rokas, Serinales M. Groenew., Hittinger, Opulente & A. Rokas; Saccharomycetes: Phaffomycetales M. Groenew., Hittinger, Opulente & A. Rokas, Saccharomycodales M. Groenew., Hittinger, Opulente & A. Rokas; Sporopachydermiomycetes: Sporopachydermiales M. Groenew., Hittinger, Opulente & A. Rokas; Trigonopsidomycetes: Trigonopsidales Groenewald M, Hittinger CT, Bensch K, Opulente DA, Shen X-X, Li Y, Liu C, LaBella AL, Zhou X, Limtong S, Jindamorakot S, Gonçalves P, Robert V, Wolfe KH, Rosa CA, Boekhout T, Čadež N, Péter G, Sampaio JP, Lachance M-A, Yurkov AM, Daniel H-M, Takashima M, Boundy-Mills K, Libkind D, Aoki K, Sugita T, Rokas A (2023). A genome-informed higher rank classification of the biotechnologically important fungal subphylum New families: Alaninales: Pachysolenaceae M. Groenew., Hittinger, Opulente & A. Rokas; Pichiales: Pichiaceae M. Groenew., Hittinger, Opulente & A. Rokas; Sporopachydermiales: Sporopachydermiaceae M. Groenew., Hittinger, Opulente & A. Rokas. Citation: Groenewald M, Hittinger CT, Bensch K, Opulente DA, Shen X-X, Li Y, Liu C, LaBella AL, Zhou X, Limtong S, Jindamorakot S, Gonçalves P, Robert V, Wolfe KH, Rosa CA, Boekhout T, Čadež N, Péter G, Sampaio JP, Lachance M-A, Yurkov AM, Daniel H-M, Takashima M, Boundy-Mills K, Libkind D, Aoki K, Sugita T, Rokas A (2023). A genome-informed higher rank classification of the biotechnologically important fungal subphylum Saccharomycotina . Studies in Mycology 105 : 1-22. doi: 10.3114/sim.2023.105.01 This study is dedicated to the memory of Cletus P. Kurtzman (1938-2017), a pioneer of yeast taxonomy., Competing Interests: Antonis Rokas is a scientific consultant for LifeMine Therapeutics, Inc., (© 2023 Westerdijk Fungal Biodiversity Institute.)

To solve the research-practice gap and take one step forward toward using big data with real-world evidence, the present study aims to adopt a novel method using machine learning to pool findings from meta-analyses and predict the change of countermovement jump. The data were collected through a total of 124 individual studies included in 16 recent meta-analyses. The performance of four selected machine learning algorithms including support vector machine, random forest (RF) ensemble, light gradient boosted machine, and the neural network using multi-layer perceptron was compared. The RF yielded the highest accuracy (mean absolute error: 0.071 cm; R 2 : 0.985). Based on the feature importance calculated by the RF regressor, the baseline CMJ ("Pre-CMJ") was the most impactful predictor, followed by age ("Age"), the total number of training sessions received ("Total number of training&#95;session"), controlled or non-controlled conditions ("Control (no training)"), whether the training program included squat, lunge, deadlift, or hip thrust exercises ("Squat&#95;Lunge&#95;Deadlift&#95;Hipthrust&#95;True", "Squat&#95;Lunge&#95;Deadlift&#95;Hipthrust&#95;False"), or "Plyometric (mixed fast/slow SSC)", and whether the athlete was from an Asian pacific region including Australia ("Race&#95;Asian or Australian"). By using multiple simulated virtual cases, the successful predictions of the CMJ improvement are shown, whereas the perceived benefits and limitations of using machine learning in a meta-analysis are discussed.

The Dzyaloshinskii-Moriya interaction (DMI) and perpendicular magnetic anisotropy (PMA) were measured on four series of Co films (1-2.2 nm thick) grown on Pt or Au and covered with h-BN or Cu. Clean h-BN/Co interfaces were obtained by exfoliating h-BN and transferring it onto the Co film in situ in the ultra-high-vacuum evaporation chamber. By comparing h-BN and Cu-covered samples, the DMI induced by the Co/h-BN interface was extracted and found to be comparable in strength to that of the Pt/Co interface, one of the largest known values. The strong observed DMI despite the weak spin-orbit interaction in h-BN supports a Rashba-like origin in agreement with recent theoretical results. Upon combination of it with Pt/Co in Pt/Co/h-BN heterostructures, even stronger PMA and DMI are found which stabilizes skyrmions at room temperature and a low magnetic field.

The aim of this pilot study was to assess the effect of muscle contraction type on SmO2 during a dynamic contraction protocol (DYN) and a holding isometric contraction protocol (ISO) in the back squat exercise. Ten voluntary participants (age: 26.6 ± 5.0 years, height: 176.8 ± 8.0 cm, body mass: 76.7 ± 8.1 kg, and one-repetition maximum (1RM): 112.0 ± 33.1 kg) with back squat experience were recruited. The DYN consisted of 3 sets of 16 repetitions at 50% of 1RM (56.0 ± 17.4 kg), with a 120-second rest interval between sets and 2 seconds per movement cycle. The ISO consisted of 3 sets of 1 isometric contraction with the same weight and duration as the DYN (32 seconds). Through near-infrared spectroscopy (NIRS) in the vastus lateralis (VL), soleus (SL), longissimus (LG), and semitendinosus (ST) muscles, the minimum SmO2 (SmO2 min), mean SmO2 (SmO2 avg), percent change from baseline (SmO2 Δdeoxy) and time to recovery 50% of baseline value (t SmO2 50%reoxy) were determined. No changes in SmO2 avg were found in the VL, LG, and ST muscles, however the SL muscle had lower values in DYN, in the 1st set (p = 0.002) and in the 2nd set (p = 0.044). In terms of SmO2 min and ΔSmO2 deoxy, only the SL muscle showed differences (p≤0.05) and lower values in the DYN compared to ISO regardless of the set. The t SmO2 50%reoxy was higher in the VL muscle after ISO, only in the 3rd set. These preliminary data suggested that varying the type of muscle contraction in back squat with the same load and exercise time resulted in a lower SmO2 min in the SL muscle in DYN, most likely because of a higher demand for specialized muscle activation, indicating a larger oxygen supply-consumption gap., Competing Interests: The authors have declared that no competing interests exist., (Copyright: © 2023 Santarém et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.)

Pay-for-performance (P4P) has been widely applied in OECD countries to improve the quality of both primary and secondary care, and is increasingly being implemented in low- and middle-income countries. In 2011, Brazil introduced one of the largest P4P schemes in the world, the National Programme for Improving Primary Care Access and Quality (PMAQ). We critically assess the design of PMAQ, drawing on a comparison with England's quality and outcome framework which, like PMAQ, was implemented at scale relatively rapidly within a nationalised health system. A key feature of PMAQ was that payment was based on the performance of primary care teams but rewards were given to municipalities, who had autonomy in how the funds could be used. This meant the incentives felt by family health teams were contingent on municipality decisions on whether to pass the funds on as bonuses and the basis upon which they allocated the funds between and within teams. Compared with England's P4P scheme, performance measurement under PMAQ focused more on structural rather than process quality of care, relied on many more indicators, and was less regular. While PMAQ represented an important new funding stream for primary health care, our review suggests that theoretical incentives generated were unclear and could have been better structured to direct health providers towards improvements in quality of care., Competing Interests: Declaration of Competing Interest None declared., (Copyright © 2022. Published by Elsevier B.V.)

This study explored how varying the number of creative opponents affects youth players' performance during football small-sided games (SSGs). Initially, 60 players from 3 different age groups with n = 20 in each (under-9, U9; under-11, U11; and under-13, U13) were tested during SSGs to rank them according to their creative potential. Based on these rankings, four players shown to possess with a medium creative potential were assigned to the intermediate team for each age group. This team competed against a team on which players with high creative potential were progressively included (from one to finally all four players, 1C to 4C) during a 4-a-side plus goalkeeper SSG. The results showed that U9 players increased the individual space explored when facing more creative opponents (p = .012), while the U11 (p = .026) and U13 (p < .001) only increased when facing 2C and 3C. Playing against more creative opponents induced more variability in the distance to own (p = .046) and the opponent team's centroid (p = .046) in the U9, regularity in the U11 (team centroid, p < .001; opponents' centroid, p = .001) and lower regularity in the U13 (team centroid, p = .013 opponents' centroid, p = .009). These distinct movement patterns seemed to reflect the differences in players' perceptual and motor skills. A higher creative score was found against 1C (vs 2C and 4C, p = .006) and 3C (vs 4C, p = .006) in the U9. The results from the effect sizes indicated higher values on the players' attempts, fluency and versatility when facing 1C to 3C, whereas a clear decrease was observed against 4C for all age groups. Hence, moderate and high demanding scenarios prompt the emergence of new behaviours while extremely high demanding scenarios (i.e. 4C) seem to constrain all the creativity components. Overall, adding creative players mostly impacts the team's collective behaviour rather than the players' creativity-related skills., (Copyright © 2022 Elsevier B.V. All rights reserved.)

Data concerning the combined effect of diet and exercise interventions on overall health in the elderly are scarce. The MED-E project's primary aim is to assess the effect of the different 3-month sustainable healthy diet (SHD) and multicomponent training (MT) interventions on several health outcomes in the elderly. A quasi-experimental study assigned older adults into four groups: (1) SHD, (2) MT, (3) SHD + MT, or (4) control group (CG). The SHD intervention included a weekly offer of a mixed food supply and individual and group nutritional sessions on the principles of an SHD. The MT groups were submitted to 50-min exercise sessions three times a week. The primary outcomes were blood biomarkers and metabolic profile alterations that were assessed pre- and post-intervention. Additionally, data on dietary intake and nutritional adequacy, physical fitness, body composition and anthropometry, cognitive function, quality of life, and geographical data were assessed at the same time points. The MED-E project's study protocol and future results will add to knowledge about the importance and beneficial contribution of combined SHD and MT interventions on healthy ageing policies.

The risk of dehydration in older adults with neurocognitive disorder (NCD) is controversial. The purpose of this study was to assess hydration status, its determinants, and water intake sources in older adults with NCD. A sample of 30 participants (≥60 years) was included. Sociodemographic, clinical data and one 24-h urine sample were collected. Urinary osmolality, sodium, potassium, volume, and creatinine were quantified. Inadequate hydration status corresponded to urine osmolality > 500 mOsm/Kg, or a negative Free Water Reserve (FWR). Two 24-h food recalls were used to assess dietary intake and water sources. The adequacy of total water intake (TWI) was estimated according to EFSA. The contribution of food and beverages to TWI was calculated, and their associations with the urinary osmolality median were tested. Of the total number of participants, 30% were classified as having inadequate hydration status, with no differences between sexes. Regarding TWI, 68.4% of women and 77.8% of men did not reach the reference values. Water (23%), followed by soup (17%), contributed the most to TWI, while vegetables (2%) and alcoholic/other beverages (3%) contributed the least. According to the median urinary osmolality, there was no significant difference in sociodemographic/clinical characteristics. It is critical not to overlook hydration in this vulnerable population.

Competing Interests: Author FC was employed by the company Manchester City Football Club. Authors JV and FG were employed by the company Fútbol Club Barcelona. The remaining authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

This study aimed to investigate how weekly training load constrains the performance of players and teams in official futsal competitions. Data from a professional male team were collected during two seasons (46 weeks). The applied monitoring system analysed the training load (as measured by session perceived exertion, sRPE), the total recovery status (TQR), the well-being score (WBs) and the variability of neuromuscular performance during each week (CMJ-cv). In addition, the performance was assessed for all the matches. A path analysis model was performed to test the associations across variables. Results from the path analysis model revealed that it explains 31% of the teams' performance. In general, the results show that previous team performance has no significant effects on the training week. A significant negative relationship was found between CMJ-cv and match performance ( β = -.34; CI95% -.359 to -.070), as well as a significant negative relationship between players' match performance and the team's match performance ( β = -.55; CI95% -.292 to .740). Regarding indirect effects, only a negative association between CMJ-cv and team match performance via players' match performance ( β = -.19; CI95% -.342 to -.049) was identified. The small variation of the weekly CMJ (CMJ-cv) seems to be a key variable to monitor and explain both player and team performance. Based on this model, and only looking at the physical variables, it was possible to explain 31% of the team's performance. Longitudinal and multi-team studies should be conducted to integrate other technical, tactical and psychological variables that allow the level of understanding of players' and teams' performance to be improved., Competing Interests: The authors declare no conflict of interest., (Copyright © Biology of Sport 2023.)

The distribution of the high concentrations of arsenic (As) and fluoride (F - ) in groundwater on a Pan-European scale could be explained by the geological European context (lithology and structural faults). To test this hypothesis, seventeen countries and eighteen geological survey organizations (GSOs) have participated in the dataset. The methodology has used the HydroGeoToxicity (HGT) and the Baseline Concentration (BLC) index. The results prove that most of the waters considered in this study are in good conditions for drinking water consumption, in terms of As and/or F - content. A low proportion of the analysed samples present HGT≥ 1 levels (4% and 7% for As and F - , respectively). The spatial distribution of the highest As and/or F - concentrations (via BLC values) has been analysed using GIS tools. The highest values are identified associated with fissured hard rock outcrops (crystalline rocks) or Cenozoic sedimentary zones, where basement fractures seems to have an obvious control on the distribution of maximum concentrations of these elements in groundwaters., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2022. Published by Elsevier Inc.)

Reports of Gram-negative bacteria harboring multiple carbapenemase genes have increased in South America, leading to an urgent need for appropriate microbiological diagnosis. We evaluated phenotypic methods for detecting Klebsiella pneumoniae carbapenemase 2 (KPC-2) and New Delhi metallo-β-lactamase-1 (NDM-1) coexpression in members of the K. pneumoniae complex (i.e., K. pneumoniae, K. quasipneumoniae, and K. variicola) isolated from human and animal hosts, based on inhibition of ceftazidime-avibactam (CZA) and aztreonam (ATM) by dipicolinic acid (DPA), EDTA, or avibactam (AVI). While the presence of bla KPC-2 and bla NDM-1 genes was confirmed by whole-genome sequencing, PCR, and/or GeneXpert, coexpression was successfully detected based on the following: (i) a ≥5-mm increase in the zone diameter of ATM (30 µg) disks plus AVI (4 or 20 µg) and ≥4-mm and ≥10-mm increases in the zone diameters for "CZA 50" (30 µg ceftazidime [CAZ] and 20 µg AVI) and "CZA 14" (10 µg CAZ and 4 µg AVI) disks, respectively, when we added DPA (1 mg/disk) or EDTA (5 mM) in a combined disk test (CDT); (ii) a positive ghost zone (synergism) between ATM (30 µg) and CZA 50 disks and between CZA 50 and DPA (1 mg) disks, using the double-disk synergy test (DDST) at a disk-disk distance of 2.5 cm; (iii) ≥3-fold MIC reductions of ATM and CZA in the presence of AVI (4 µg/mL), DPA (500 µg/mL), or EDTA (320 µg/mL); and (iv) immunochromatography. Although our results demonstrated that inhibition by AVI, DPA, and EDTA may provide simple and inexpensive methods for the presumptive detection of coexpression of KPC-2 and NDM-1 in members of the K. pneumoniae complex, additional studies are necessary to confirm the accuracy of these methodologies by testing other Gram-negative bacterial species and other KPC and NDM variants coexpressed by WHO critical priority pathogens detected worldwide. IMPORTANCE Alerts regarding the emergence and increase of combinations of carbapenemases in Enterobacterales in Latin America and the Caribbean have recently been issued by PAHO and WHO, emphasizing the importance of appropriate microbiological diagnosis and the effective and articulated implementation of infection prevention and control programs. In this study, we evaluated methods based on inhibition of ceftazidime (CAZ), ceftazidime-avibactam (CZA), and aztreonam (ATM) by dipicolinic acid (DPA), EDTA, and avibactam (AVI) inhibitors for the identification of KPC-2- and NDM-1-coexpression in members of the K. pneumoniae complex recovered from human and animal hosts. Our results demonstrate that inhibition by AVI, DPA, and EDTA may provide simple and inexpensive methods for the presumptive detection of coexpression of KPC-2 and NDM-1 in members of the K. pneumoniae complex.

The current technological advances have pushed the quantification of exercise intensity to new era of physical exercise sciences. Monitoring physical exercise is essential in the process of planning, applying, and controlling loads for performance optimization and health. A lot of research studies applied various statistical approaches to estimate various physiological indices, to our knowledge, no studies found to investigate the relationship of facial color changes and increased exercise intensity. The aim of this study was to develop a non-contact method based on computer vision to determine the heart rate and, ultimately, the exercise intensity. The method was based on analyzing facial color changes during exercise by using RGB, HSV, YCbCr, Lab, and YUV color models. Nine university students participated in the study (mean age = 26.88 ± 6.01 years, mean weight = 72.56 ± 14.27 kg, mean height = 172.88 ± 12.04 cm, six males and three females, and all white Caucasian). The data analyses were carried out separately for each participant (personalized model) as well as all the participants at a time (universal model). The multiple auto regressions, and a multiple polynomial regression model were designed to predict maximum heart rate percentage (maxHR%) from each color models. The results were analyzed and evaluated using Root Mean Square Error (RMSE), F-values, and R-square. The multiple polynomial regression using all participants exhibits the best accuracy with RMSE of 6.75 (R-square = 0.78). Exercise prescription and monitoring can benefit from the use of these methods, for example, to optimize the process of online monitoring, without having the need to use any other instrumentation.

Aim: The aim of this study was to examine the effect of bio-banding on technical and tactical markers of talent identification in 11- to 14-year-old academy soccer players., Methods: Using a repeated measures design, 92 players were bio-banded using percentage of estimated adult stature attainment (week 1), maturity-offset (week 2) and a mixed-maturity method (week 3). All players contested five maturity (mis)matched small-sided games with technical and tactical variables measured. Data were analysed using a series of Bayesian hierarchical models, fitted with different response distributions and different random and fixed effect structures., Results: Despite differences during maturity-matched bio-banding for post-peak height velocity players, very few tactical differences were evident during the remaining maturity-matched and mis-matched fixtures for both banding methods. In fact, the results showed no consistent differences across both banding methods for practitioner and video analysis-derived technical performance characteristics during maturity matched and mis-matched fixtures. Both bio-banding methods explained similar levels of variance across the measured variables., Conclusion: Maturity-matched bio-banding had some effect on both technical and tactical characteristics of players during maturity-matched bio-banded formats. That said, this trend remained during maturity mis-matched bio-banded formats which restricts the conclusions that can be made regarding the effectiveness of bio-banding to manipulate technical and tactical measures in academy soccer players.

In this paper, we present the results of a 6-year experiment in space that studied the effects of radiation in Gallium Nitride (GaN) electronics in geostationary orbit. Four GaN transistors in a Colpitts oscillator configuration were flown in the Component Technology Test-Bed aboard the Alphasat telecommunication satellite. A heuristic analysis was performed by observing the variation in the power output of the oscillators with the total ionizing dose gathered during the mission. The total ionizing dose was measured with a Radiation Sensing Field Effect Transistors (RadFET) placed close to the GaN devices. The experiment showed that GaN is a robust technology that can be used in the space radiation environment of a geostationary orbit. The work presented here starts with a brief introduction of the subject, the motivation, and the main goal. This is followed by the description of the experimental setup, including the details of the oscillator design and simulations, as well as the implementation of the test-bed and the Components Technology Test-Bed. Finally, the results obtained during the 6 years of experience in space are discussed., (© 2022. The Author(s).)

This study aimed to explore how youth players' physical, technical and positional performance may be affected by visual occlusion when playing under different SSG pitch sizes. Under-15 players performed two experimental scenarios: a) normal situation, without visual occlusion; b) visual occlusion, by using an eye patch in the eye corresponding to the dominant foot. These scenarios were tested in a small (40x30m) and a larger pitch (50x35m). Players' positional data was used to compute tactical and time-motion variables. In addition, technical analysis was comprised using video footage. Playing with visual occlusion in the larger pitch size induced higher distance covered while walking but lower running distance (p < .05). Although no statistically significant effects were identified between the normal and visual occlusion conditions for the tactical behaviour and technical performance a lower number of successful passes (small to moderate effect sizes) and higher regularity in the distance to the opponent's team centroid (moderate effect size) were found with visual occlusion. Players covered more distance and achieved higher maximum speed in the larger compared to the small pitch (moderate to large effect size, p < .05), while also increasing their distance to both team's centroid and increasing the regularity to these distances (moderate to large effect size, p < .05). Overall, despite similar effects for tactical and technical variables, some important practical information can be depicted. Accordingly, coaches may use the visual occlusion to promote more stable and regular behaviors while decreasing the physical demands. Larger pitches may be used to increase the distance between players' and teams, as well as to induce higher physical load in both the normal and visual occlusion conditions. From the technical perspective, coaches may design smaller pitches to emphasize the use of the non-dominant foot during the occlusion scenario and promote the pass during the normal scenario., Competing Interests: the authors have declared that no competing interests exist.

Background: Pay-for-performance (P4P) programmes to incentivise health providers to improve quality of care have been widely implemented globally. Despite intuitive appeal, evidence on the effectiveness of P4P is mixed, potentially due to differences in how schemes are designed. We exploited municipality variation in the design features of Brazil's National Programme for Improving Primary Care Access and Quality (PMAQ) to examine whether performance bonuses given to family health team workers were associated with changes in the quality of care and whether the size of bonus mattered., Methods and Findings: For this quasi-experimental study, we used a difference-in-differences approach combined with matching. We compared changes over time in the quality of care delivered by family health teams between (bonus) municipalities that chose to use some or all of the PMAQ money to provide performance-related bonuses to team workers with (nonbonus) municipalities that invested the funds using traditional input-based budgets. The primary outcome was the PMAQ score, a quality of care index on a scale of 0 to 100, based on several hundred indicators (ranging from 598 to 660) of health care delivery. We did one-to-one matching of bonus municipalities to nonbonus municipalities based on baseline demographic and economic characteristics. On the matched sample, we used ordinary least squares regression to estimate the association of any bonus and size of bonus with the prepost change over time (between November 2011 and October 2015) in the PMAQ score. We performed subgroup analyses with respect to the local area income of the family health team. The matched analytical sample comprised 2,346 municipalities (1,173 nonbonus municipalities; 1,173 bonus municipalities), containing 10,275 family health teams that participated in PMAQ from the outset. Bonus municipalities were associated with a 4.6 (95% CI: 2.7 to 6.4; p < 0.001) percentage point increase in the PMAQ score compared with nonbonus municipalities. The association with quality of care increased with the size of bonus: the largest bonus group saw an improvement of 8.2 percentage points (95% CI: 6.2 to 10.2; p < 0.001) compared with the control. The subgroup analysis showed that the observed improvement in performance was most pronounced in the poorest two-fifths of localities. The limitations of the study include the potential for bias from unmeasured time-varying confounding and the fact that the PMAQ score has not been validated as a measure of quality of care., Conclusions: Performance bonuses to family health team workers compared with traditional input-based budgets were associated with an improvement in the quality of care., Competing Interests: The authors have declared that no competing interests exist.

This study aimed to compare under-18 association football players' performance (age = 17.7±1.0 years; playing experience = 9.0 ± 3.2 years) when manipulating the number of teammates and opponents during football game-based practices. Time-motion, individual and tactical-related variables were monitored when manipulating conditions with different number of teammates and opponents (11vs11, No-Sup, No-Inf; 11vs12, Low-Sup, Low-Inf; 11vs13, Mod-Sup, Mod-Inf; and 11vs14, High-Sup, High-Inf). Results showed that adding teammates promoted increases in the longitudinal synchronization from No-Sup to Mod-Sup (Cohen's d with 95% of confidence intervals: 0.25 [0.12; 0.39]; p < .001) and High-Sup (0.61 [0.41; 0.82]; p < .001), while decreases in the distance to the nearest teammate, both in the offensive and defensive phases (p < .001 and p = .005, respectively). In addition, it was observed lower distance covered while running when playing in High-Sup compared to No-Sup (0.30 [-0.01; 0.61]; p = .002) during the defensive phase. Attacking in numerical inferiority promoted a higher variability in the distance to the nearest teammate from No-Inf to High-Inf (0.83 [0.27; 1.38]; p = .044), while decreasing the physical demands, specifically distance covered while running (-0.49 [-0.99; 0.01]; p = .039). In turn, defending, mainly in high-inferiority, increased the total distance covered compared to No-Inf (0.61 [0.30; 0.91]; p < .001) and led to a decrease in the distance to the nearest teammate (-0.90 [-1.35; -0.44]; p = .002). Overall, coaches may manipulate the number of teammates and opponents to promote distinct effects at the level of cooperation and opposition dynamical interactions., Competing Interests: The authors report no conflict of interest.

Competing Interests: Competing interests: None declared.

Next-generation sequencing (NGS) is an indispensable tool in antibody discovery projects. However, the limits on NGS read length make it difficult to reconstruct full antibody sequences from the sequencing runs, especially if the six CDRs are randomized. To overcome that, we took advantage of Illumina's cluster mapping capabilities to pair non-overlapping reads and reconstruct full Fab sequences with accurate V L :V H pairings. The method relies on in silico cluster coordinate information, and not on extensive in vitro manipulation, making the protocol easily deployable and less prone to PCR-derived errors. This work maintains the throughput necessary for antibody discovery campaigns, and a high degree of fidelity, which potentiates not only phage-display and synthetic library-based discovery methods, but also the NGS-driven analysis of naïve and immune libraries., Competing Interests: The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (© 2022 The Author(s).)

This study aims to investigate how exposure to poverty, food insecurity, and abuse at older ages relates to health outcomes. A questionnaire collecting data on sociodemographic and economic characteristics, health status, depressive symptoms, food insecurity, and abuse was administered to a sample of 677 older adults. Logistic regression was used to quantify the association of poverty, food insecurity, and abuse with perceived health status and depressive symptoms. If the older person only reported experiences of abuse, it was more likely to report the presence of depressive symptoms, even after adjustment for covariates. If it was only reported the experience of food insecurity, it was more likely to report a worse health status. Older people exposed to at least two factors of vulnerability were significantly more likely to report (very) poor perceived health status (OR: 7.11, 95% CI: 2.77-18.25) and the presence of relevant depressive symptoms (OR: 4.34; 95% CI: 2.04-9.22). Thus, the combined effect of vulnerabilities was significantly associated with worse health among older people. Public health policies to mitigate these adverse exposures should be developed to promote health and well-being in this population.

Abstract: Weldon, A, Duncan, MJ, Turner, A, LaPlaca, D, Sampaio, J, and Christie, CJ. Practices of strength and conditioning coaches: a snapshot from different sports, countries, and expertise levels. J Strength Cond Res 36(5): 1335-1344, 2022-This study describes the practices of strength and conditioning coaches (SCCs) from different sports, countries, and expertise levels. One hundred fifty-six SCCs (31.9 ± 8.9 years old) completed an online survey, consisting of 40 questions (36 fixed response and 4 open-ended), with 8 sections as follows: (a) background information, (b) muscular strength and power development, (c) speed development, (d) plyometrics, (e) flexibility development, (f) physical testing, (g) technology use, and (h) programming and any additional comments. Responses were received from 48 sports and 17 countries. This study provides exploratory evidence incorporating responses primarily in soccer (45%), track and field (30%), volleyball (23%), golf (17%), and tennis (17%). A bachelor's degree or higher were held by 99% of SCCs, of which 94% were in a sports science-related field, and 71% held a strength and conditioning related certification or accreditation. Periodization strategies and physical testing were used by 96% and 94% of SCCs respectively. The hang clean (82%), power clean (76%), and clean high pull (63%) were the most prescribed Olympic weightlifting exercises. Multiple hops/lunges (84%) were the most prescribed plyometrics exercises. For open-ended questions, 40% of SCCs wanted to integrate more technology into their programs and 30% believed technology will be the main future trend. Strength and conditioning coaches from different sports, countries, and expertise levels can use the information presented in this study to review their current practices and provide a source of new ideas for diversifying or modifying future practices., (Copyright © 2020 National Strength and Conditioning Association.)

Purpose: To investigate the match-to-match variation of physical performance during official congested fixtures in elite futsal players., Methods: Physical performance was measured by external and internal load metrics in 12 elite male futsal players. Two periods with 3 matches within 4 days were analyzed. The variation in physical performance of the players during matches was analyzed using the latent growth curve modeling that estimated interindividual and intraindividual growth paths., Results: Playing time had a significant effect on physical performance growth with significant paths of interindividual and intraindividual variability. Players who competed for more time revealed lower initial levels (ie, first match) of total distance covered (β = -0.62), high-speed running (β = -0.18), accelerations (β = -0.31), decelerations (β = -0.44), and session rate of perceived exertion (β = 0.81) than players who competed for less time (P < .05). In addition, players who competed for more time revealed higher increases in total distance covered (β = 0.47), high-speed running (β = 0.16), and session rate of perceived exertion (β = 0.66) and lower increases in accelerations (β = -0.21) and decelerations (β = -0.58) than players who competed for less time from the first to the third match (P < .05)., Conclusions: Congested fixtures did not affect physical performance in elite futsal players. Playing time showed to be a key performance factor. There was a considerable heterogeneity in the responsiveness to physical performance over congested fixtures, suggesting an analysis of individual variability to evaluate real changes in match performance, training intensity, and workload.

Background: Over the last years there have been a wide body of research exploring the best strategies to promote acute enhancements in players' performance. Despite that, most studies have been focused on adult and elite players, and different results may be identified when considering players from lower levels of performance and belonging to youth categories. In addition, most studies conducted in this domain focused in repetitive movement patterns, and while adding variability has been considered as a useful approach to enhance players' performance at short and long-term perspectives, less is known regarding it applicability to acute enhance players physical performance. Therefore, this study aimed to compare the acute enhancement effects of performing the half-squat in a flywheel ergometer between a more-repetitive approach (low noise) and a more variable approach (differential learning, high noise) in youth soccer players., Methods: A total of sixteen players (age = 16.2 ± 0.6 years) was exposed to four conditions in a randomized order: (1) repetitive intervention for 30 s; (2) repetitive intervention for 10-min; (3) differential learning intervention for 30 s; (4) differential learning intervention for 10-min. Each condition consisted in 3 sets of 6 repetitions of eccentric half squats performed in a flywheel ergometer. Countermovement jump, 10 m and 30 m linear sprint, and change-of-direction ability were measured every session at baseline (pre-test) and after each protocol (post-test)., Results: No potentiation effect was observed overall with any of the interventions. In addition, no differences between protocols were found for sprinting. However, the repetitive intervention impaired jumping performance for both 30 s (small effects, p ≤ .05) and 10-min intervals (small effects, p ≤ .05), as well as in the change-of-direction task for 30 s (p ≤ .05)., Conclusions: These results may be due to the players' low experience in eccentric flywheel training. Despite these findings, individual potentiation responses emerged from both protocols when considering the individual responses, reinforcing the need to establish more personalized approaches., (© 2022. The Author(s).)

This study explored how manipulating the colour of training vests affects footballers' individual and collective performance during a Gk+6vs6+Gk medium-sided game. A total of 21 under-17 years old players were involved in three experimental conditions in a random order for a total of four days: i) CONTROL, two teams using two different colour vests; ii) SAME, both teams wearing blue vests; iii) MIXED, all 6 players per team wore different colour vests. Players' positional data was used to compute time-motion and tactical-related variables, while video analysis was used to collect technical variables. Further, these variables were synchronized with spatiotemporal data allowing to capture ball-related actions in a horizontal 2D plan. All variables were analysed from the offensive and defensive perspective. From the offensive perspective, players performed more and further shots to goal during the CONTROL than in SAME and MIXED (small effects) conditions, with a decreased distance to the nearest defender (small effects). While defending, results revealed lower distance to the nearest teammate (small effects) in the CONTROL than in the SAME and MIXED conditions, and higher team longitudinal synchronization (small effects). In addition, the CONTROL showed in general lower values of team width while defending than in the other 2 conditions. Overall, coaches may use the CONTROL condition to emphasize offensive performance and defensive behaviour over the longitudinal direction with increased physical demands. In turn, coaches may use the manipulation of players vests to emphasize defensive performance, as players seem to behave more cohesively under such scenarios., Competing Interests: The authors have declared that no competing interests exist.

The aim of this study was to identify how positioning the goals in diagonal configurations on the pitch modifies the external training load and the tactical behaviour of young football players during small-sided games. Four teams of five outfield players and a goalkeeper played six small-sided games of five minutes' duration in three different scenarios: 1) Control: goals placed one in front of the other; 2) Right diagonal goals: goals placed in the right-hand corner of the offensive half-pitch; and 3) Left diagonal goals: goals placed in the left-hand corner of the offensive half-pitch. The positioning-derived data from each player were collected with 10-Hz GPS units and were used to compute external load and tactical variables. Regarding the external load variables, differences were mainly focused on distance covered while walking in defence and game pace (variability), with higher values for the diagonal scenarios. Also, the length/width ratios in offence and defence were most likely lower in diagonal scenarios. In conclusion, the results showed that players' adaptations to the environmental constraints of positioning the goals diagonally were the enhancement of the width team variable and the variability of the length., Competing Interests: The authors reported no conflict of interest., (Copyright © Biology of Sport 2021.)

In the field of sports science, the recovery umbrella is a trending topic, and even more so in the world of elite sports. This is evidenced by the significant increase in scientific publications during the last 10 years as teams look to find a competitive edge. Recovery is recognized to be an integral component to assist athlete preparation in the restoration of physical and psychological function, and subsequently, performance in elite team sports athletes. However, the importance of recovery in team staff members (sports coaches and performance staff) in elite sports appears to be a forgotten element. Given the unrelenting intense nature of daily tasks and responsibilities of team staff members, the elite sports environment can predispose coaches to increased susceptibility to psycho-socio physiological fatigue burden, and negatively affect health, wellbeing, and performance. Therefore, the aim of this opinion was to (1) develop an educational recovery resource for team staff members, (2) identify organizational task-specific fatigue indicators and barriers to recovery and self-care in team staff members, and (3) present recovery implementation strategies to assist team staff members in meeting their organizational functions. It is essential that we do not forget the coaching and performance staff in the recovery process.