5 results on '"Santos YV"'
Search Results
2. Mansonella ozzardi mitogenome and pseudogene characterisation provides new perspectives on filarial parasite systematics and CO-1 barcoding.
- Author
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Crainey JL, Marín MA, Silva TRRD, de Medeiros JF, Pessoa FAC, Santos YV, Vicente ACP, and Luz SLB
- Subjects
- Animals, Genomics methods, Humans, Phylogeny, RNA, Ribosomal genetics, RNA, Ribosomal, 5S genetics, Electron Transport Complex IV genetics, Genome, Mitochondrial, Mansonella classification, Mansonella genetics, Mansonelliasis parasitology, Pseudogenes
- Abstract
Despite the broad distribution of M. ozzardi in Latin America and the Caribbean, there is still very little DNA sequence data available to study this neglected parasite's epidemiology. Mitochondrial DNA (mtDNA) sequences, especially the cytochrome oxidase (CO1) gene's barcoding region, have been targeted successfully for filarial diagnostics and for epidemiological, ecological and evolutionary studies. MtDNA-based studies can, however, be compromised by unrecognised mitochondrial pseudogenes, such as Numts. Here, we have used shot-gun Illumina-HiSeq sequencing to recover the first complete Mansonella genus mitogenome and to identify several mitochondrial-origin pseudogenes. Mitogenome phylogenetic analysis placed M. ozzardi in the Onchocercidae "ONC5" clade and suggested that Mansonella parasites are more closely related to Wuchereria and Brugia genera parasites than they are to Loa genus parasites. DNA sequence alignments, BLAST searches and conceptual translations have been used to compliment phylogenetic analysis showing that M. ozzardi from the Amazon and Caribbean regions are near-identical and that previously reported Peruvian M. ozzardi CO1 reference sequences are probably of pseudogene origin. In addition to adding a much-needed resource to the Mansonella genus's molecular tool-kit and providing evidence that some M. ozzardi CO1 sequence deposits are pseudogenes, our results suggest that all Neotropical M. ozzardi parasites are closely related.
- Published
- 2018
- Full Text
- View/download PDF
3. The man who feels two hearts: the different pathways of interoception.
- Author
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Couto B, Salles A, Sedeño L, Peradejordi M, Barttfeld P, Canales-Johnson A, Dos Santos YV, Huepe D, Bekinschtein T, Sigman M, Favaloro R, Manes F, and Ibanez A
- Subjects
- Adult, Cognition Disorders diagnosis, Cognition Disorders etiology, Electrocardiography, Electroencephalography, Female, Heart Failure surgery, Heart Rate physiology, Humans, Male, Neuropsychological Tests, Young Adult, Afferent Pathways physiology, Cerebral Cortex physiology, Emotions physiology, Heart Failure complications, Heart Failure psychology, Heart-Assist Devices psychology, Interoception
- Abstract
Recent advances in neuroscience have provided new insights into the understanding of heart-brain interaction and communication. Cardiac information to the brain relies on two pathways, terminating in the insular cortex (IC) and anterior cingulate cortex (ACC), along with the somatosensory cortex (S1-S2). Interoception relying on these neuroanatomical pathways has been shown to modulate social cognition. We report the case study of C.S., a patient with an 'external heart' (an extracorporeal left-univentricular cardiac assist device, LVAD). The patient was assessed with neural/behavioral measures of cardiac interoception complemented by neuropsychological and social cognition measures. The patient's performance on the interoception task (heartbeat detection) seemed to be guided by signals from the artificial LVAD, which provides a somatosensory beat rather than by his endogenous heart. Cortical activity (HEP, heartbeat-evoked potential) was found decreased in comparison with normal volunteers, particularly during interoceptive states. The patient accurately performed several cognitive tasks, except for interoception-related social cognition domains (empathy, theory of mind and decision making). This evidence suggests an imbalance in the patient's cardiac interoceptive pathways that enhances sensation driven by the artificial pump over that from the cardiac vagal-IC/ACC pathway. A patient with two hearts, one endogenous and one artificial, presents a unique opportunity to explore models of interoception and heart-brain interaction., (© The Author (2013). Published by Oxford University Press. For Permissions, please email: journals.permissions@oup.com.)
- Published
- 2014
- Full Text
- View/download PDF
4. Crystal structure of a Schistosoma mansoni septin reveals the phenomenon of strand slippage in septins dependent on the nature of the bound nucleotide.
- Author
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Zeraik AE, Pereira HM, Santos YV, Brandão-Neto J, Spoerner M, Santos MS, Colnago LA, Garratt RC, Araújo AP, and DeMarco R
- Subjects
- Animals, Binding Sites, Calorimetry, Catalysis, Cell Membrane metabolism, Crystallography, X-Ray, Escherichia coli metabolism, GTP Phosphohydrolases chemistry, Guanosine Diphosphate chemistry, Guanosine Triphosphate chemistry, Hydrolysis, Magnesium chemistry, Magnetic Resonance Spectroscopy, Nucleotides chemistry, Protein Structure, Secondary, Protein Structure, Tertiary, Recombinant Proteins chemistry, Thermodynamics, Water chemistry, Schistosoma mansoni chemistry, Septins chemistry
- Abstract
Septins are filament-forming GTP-binding proteins involved in important cellular events, such as cytokinesis, barrier formation, and membrane remodeling. Here, we present two crystal structures of the GTPase domain of a Schistosoma mansoni septin (SmSEPT10), one bound to GDP and the other to GTP. The structures have been solved at an unprecedented resolution for septins (1.93 and 2.1 Å, respectively), which has allowed for unambiguous structural assignment of regions previously poorly defined. Consequently, we provide a reliable model for functional interpretation and a solid foundation for future structural studies. Upon comparing the two complexes, we observe for the first time the phenomenon of a strand slippage in septins. Such slippage generates a front-back communication mechanism between the G and NC interfaces. These data provide a novel mechanistic framework for the influence of nucleotide binding to the GTPase domain, opening new possibilities for the study of the dynamics of septin filaments.
- Published
- 2014
- Full Text
- View/download PDF
5. Motor-language coupling: direct evidence from early Parkinson's disease and intracranial cortical recordings.
- Author
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Ibáñez A, Cardona JF, Dos Santos YV, Blenkmann A, Aravena P, Roca M, Hurtado E, Nerguizian M, Amoruso L, Gómez-Arévalo G, Chade A, Dubrovsky A, Gershanik O, Kochen S, Glenberg A, Manes F, and Bekinschtein T
- Subjects
- Analysis of Variance, Antiparkinson Agents therapeutic use, Cognition Disorders etiology, Cognition Disorders psychology, Comprehension physiology, Data Interpretation, Statistical, Educational Status, Electroencephalography, Evoked Potentials, Motor physiology, Female, Hand physiology, Humans, Image Processing, Computer-Assisted, Magnetic Resonance Imaging, Male, Middle Aged, Motor Cortex physiopathology, Nervous System Diseases physiopathology, Nervous System Diseases psychology, Neurodegenerative Diseases physiopathology, Neurodegenerative Diseases psychology, Parkinson Disease psychology, Reaction Time physiology, Transcranial Magnetic Stimulation, Cerebral Cortex physiopathology, Language, Movement physiology, Parkinson Disease physiopathology
- Abstract
Language and action systems are functionally coupled in the brain as demonstrated by converging evidence using Functional magnetic resonance imaging (fMRI), electroencephalography (EEG), transcranial magnetic stimulation (TMS), and lesion studies. In particular, this coupling has been demonstrated using the action-sentence compatibility effect (ACE) in which motor activity and language interact. The ACE task requires participants to listen to sentences that described actions typically performed with an open hand (e.g., clapping), a closed hand (e.g., hammering), or without any hand action (neutral); and to press a large button with either an open hand position or closed hand position immediately upon comprehending each sentence. The ACE is defined as a longer reaction time (RT) in the action-sentence incompatible conditions than in the compatible conditions. Here we investigated direct motor-language coupling in two novel and uniquely informative ways. First, we measured the behavioural ACE in patients with motor impairment (early Parkinson's disease - EPD), and second, in epileptic patients with direct electrocorticography (ECoG) recordings. In experiment 1, EPD participants with preserved general cognitive repertoire, showed a much diminished ACE relative to non-EPD volunteers. Moreover, a correlation between ACE performance and action-verb processing (kissing and dancing test - KDT) was observed. Direct cortical recordings (ECoG) in motor and language areas (experiment 2) demonstrated simultaneous bidirectional effects: motor preparation affected language processing (N400 at left inferior frontal gyrus and middle/superior temporal gyrus), and language processing affected activity in movement-related areas (motor potential at premotor and M1). Our findings show that the ACE paradigm requires ongoing integration of preserved motor and language coupling (abolished in EPD) and engages motor-temporal cortices in a bidirectional way. In addition, both experiments suggest the presence of a motor-language network which is not restricted to somatotopically defined brain areas. These results open new pathways in the fields of motor diseases, theoretical approaches to language understanding, and models of action-perception coupling., (Copyright © 2012 Elsevier Ltd. All rights reserved.)
- Published
- 2013
- Full Text
- View/download PDF
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