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A method involving chitosan-assisted magnetic-stirring-enhanced mechanical amorphous dispersion extraction was developed and utilized to extract hydrophobic anthraquinones from Rhei Radix et Rhizoma prior to ultrahigh performance liquid chromatography analysis. Incorporating natural chitosan as a dispersant facilitated the extraction of hydrophobic anthraquinones using purified water, considerably enhancing the eco-friendliness of the extraction methodology. To optimize extraction efficiency, an extensive evaluation of the crucial parameters influencing rhubarb yield was conducted. Furthermore, a response surface methodology was applied to optimize the extraction conditions. Under these optimized conditions, the method exhibited linearity ranges of 0.1-100 µg/mL, with correlation coefficients between 0.9990 and 0.9998. The method's intraday (n = 6) and interday (n = 6) precision levels were maintained at ≤3.58%, which was considered to be within acceptable limits. The computed detection and quantification limits were 16.54-24.60 and 54.91-82.04 ng/mL, respectively. Consequently, this optimized method was effectively employed to extract five specific compounds (aloe-emodin, emodin, rhein, chrysophanol, and physcion) from Rhei Radix et Rhizoma, achieving recoveries ranging from 86.43% to 102.75%., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier B.V. All rights reserved.)

An on-line enrichment and separation of multiple derivatized monosaccharides with cyclodextrin-encapsulated sweeping (CDES) by micellar electrokinetic chromatography (MEKC) was presented. Five monosaccharides (L-(-)-Mannose, D-(+)-Glucose, D-(-)-Ribose, D-(+)-Xylose, and L-(+)-Rhamnose) were derivatized with 1-phenyl-3-methyl-5-pyrazolone, subsequently concentrated and separated by MEKC. The optimized conditions were as follows: 50 mM phosphoric acid (PA), 100 mM sodium dodecyl sulfate (SDS), and 30 % (v/v) methanol in background solution; 140 s injection of sample solution containing 50 mM CD and 100 mM PA, followed by 90 s injection of 40 mM SDS solution. Under the optimized conditions, the correlation coefficients ≥ 0.9953, and the limits of detection ranged from 4.2 to 7.4 ng/mL. Relative standard deviation values ranged from 0.24-4.23 %, and sensitivity enrichment factors were in the range of 53-82 compared with typical injection (50 mbar, 3 s). The CDES-MEKC method was successfully applied to Jujube with good recoveries of 84.22-104.33 %. The method provides new ideas for the on-line enrichment and detection of trace monosaccharides and even other target analytes in foods with complex matrices., Competing Interests: Declaration of Competing Interest The authors (Huang-Fei Jin, Ying Shi, Qian-Xue Shen, Jun Cao, Li-Hong Ye) declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 Elsevier B.V. All rights reserved.)