1. Industrial chicory genome gives insights into the molecular timetable of anther development and male sterility.
- Author
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Waegneer E, Rombauts S, Baert J, Dauchot N, De Keyser A, Eeckhaut T, Haegeman A, Liu C, Maudoux O, Notté C, Staelens A, Van der Veken J, Van Laere K, and Ruttink T
- Abstract
Industrial chicory ( Cichorium intybus var. sativum ) is a biannual crop mostly cultivated for extraction of inulin, a fructose polymer used as a dietary fiber. F1 hybrid breeding is a promising breeding strategy in chicory but relies on stable male sterile lines to prevent self-pollination. Here, we report the assembly and annotation of a new industrial chicory reference genome. Additionally, we performed RNA-Seq on subsequent stages of flower bud development of a fertile line and two cytoplasmic male sterile (CMS) clones. Comparison of fertile and CMS flower bud transcriptomes combined with morphological microscopic analysis of anthers, provided a molecular understanding of anther development and identified key genes in a range of underlying processes, including tapetum development, sink establishment, pollen wall development and anther dehiscence. We also described the role of phytohormones in the regulation of these processes under normal fertile flower bud development. In parallel, we evaluated which processes are disturbed in CMS clones and could contribute to the male sterile phenotype. Taken together, this study provides a state-of-the-art industrial chicory reference genome, an annotated and curated candidate gene set related to anther development and male sterility as well as a detailed molecular timetable of flower bud development in fertile and CMS lines., Competing Interests: Authors OM and CN are employed by Chicoline, a division of Cosucra Groupe Warcoing. The remaining authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. This study received funding from Cosucra Groupe Warcoing S.A. Belgium as scholarship to EW and JV. The funder had the following involvement in the study: Cosucra Groupe Warcoing S.A. Belgium was partner in the DGA project Grant D31-1221 with the Walloon region DGARNE Belgium that initiated genome sequencing. ND and OM, affiliated with Cosucra Groupe Warcoing, created and provided the inbred line L8001., (Copyright © 2023 Waegneer, Rombauts, Baert, Dauchot, De Keyser, Eeckhaut, Haegeman, Liu, Maudoux, Notté, Staelens, Van der Veken, Van Laere and Ruttink.)
- Published
- 2023
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