17 results on '"Sun, Zhenfan"'
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2. Ag and TiO 2 nanoparticles co-modified defective zeolite TS-1 for improved photocatalytic CO 2 reduction.
- Author
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Sun Y, Li G, Gong Y, Sun Z, Yao H, and Zhou X
- Abstract
Photocatalytic CO
2 reduction into fuels has been an attractive research topic. Herein, Ag and TiO2 nanoparticles co-loaded zeolite TS-1 (Ag-TiO2 /TS-1) were synthesized by the ion-exchange and subsequently in-situ photodeposition method. The obtained Ag-TiO2 /TS-1 sample has a high surface area and rich Ti3+ -Vo defects and as well as highly dispersed Ag nanoparticles. As expected, the sample Ag-TiO2 /TS-1 not only shows high CO2 adsorption capacity, but also improves the separation efficiency of photogenerated electron-hole pairs. As a result, only CO and CH4 can be detected on the sample Ag-TiO2 /TS-1 in the photocatalytic CO2 conversion, and the competing H2 evolution can be completely suppressed, suggestive of its high selectivity. The super photocatalytic activity toward CO2 reduction can be ascribed to the synergistic effect among highly dispersed Ag nanoparticle, the support zeolite TS-1 and TiO2 with highly exposed {101} planes., (Copyright © 2020 Elsevier B.V. All rights reserved.)- Published
- 2021
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3. The computational and experimental studies on a 1, 2, 3-triazole compound and its special binding to three kinds of blood proteins.
- Author
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Li J, Feng H, Liu R, Ding G, Si H, He W, and Sun Z
- Subjects
- Algorithms, Animals, Cattle, Humans, Molecular Conformation, Protein Binding, Quantum Theory, Serum Albumin metabolism, Spectrum Analysis, Structure-Activity Relationship, Thermodynamics, Triazoles metabolism, Molecular Docking Simulation, Molecular Dynamics Simulation, Serum Albumin chemistry, Triazoles chemistry
- Abstract
A newly synthesized compound, ethyl 5-phenyl-2-(p-tolyl)-2H-1, 2, 3-triazole-4-carboxylate (EPPC) may be considered as a drug candidate and was exploited to study the structural and spectral properties by using quantum chemical calculation and multiple spectroscopic techniques. The results on theoretical spectrum of EPPC were consistent with experimental spectrum in great degree. In addition, EPPC has been as a special probe and investigated on the interactions with three kinds of blood proteins including human serum albumin (HSA), human immunoglobulin (HIgG) and bovine hemoglobin (BHb) by using UV-Vis, fluorescence spectroscopy and molecular modeling, respectively. Changes in various fluorescence and UV-Vis spectra were observed upon ligand binding along with a remarkable degree of fluorescence enhancement on complex formation under physiological condition with binding constant about 10
5 order of magnitudes, which caused the variations of conformation and microenvironment of these proteins in aqueous solution. The obtained results from the thermodynamic parameters calculated according to the van't Hoff equation indicated that the entropy change ΔS° and enthalpy change ΔH° were found to be 0.168 KJ/mol K and 22.154 KJ/mol for EPPC-HSA system, 0.284 KJ/mol K and 54.408 KJ/mol for EPPC-HIgG system, and 0.228 KJ/mol K and 37.548 KJ/mol for EPPC-BHb system, respectively, which demonstrated that the primary binding pattern is determined by hydrophobic interaction. The results of docking and molecular dynamics simulation using three proteins crystal models revealed that EPPC could bind to three proteins well into hydrophobic cavity, which showed good consistence with the spectroscopic measurements.Communicated by Ramaswamy H. Sarma.- Published
- 2020
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4. Evolution of diffusion and structure of six n-alkanes in carbon dioxide at infinite dilution over wide temperature and pressure ranges: a molecular dynamics study.
- Author
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Feng H, Gao W, Su L, Liu Y, Sun Z, and Chen L
- Abstract
Over wide temperature and pressure ranges, the molecular dynamics simulation is performed to study the mass transfer of six n-alkanes from n-C
5 H12 to n-C10 H22 in CO2 at infinite dilution by calculating the diffusion coefficients, which have not yet been measured by experiment. Meanwhile, the structural properties of these systems are explored. It is found that under different temperature and pressure conditions, the variation trends of the radial distribution functions of n-alkanes are quite different, while the variation trends of the average coordination number of n-alkanes can be divided into three types. The radius of gyration and the solvent accessible surface area are both affected by temperature and carbon chain length, but their variation trends are different, and it could explain the abnormal variation trends of the radial distribution functions and the average coordination number. Graphical abstract Over wide temperature and pressure ranges, the variation trends of the average coordination number of n-alkanes can be divided into three types.- Published
- 2019
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5. MD simulation study of the diffusion and local structure of n-alkanes in liquid and supercritical methanol at infinite dilution.
- Author
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Feng H, Gao W, Su L, Sun Z, and Chen L
- Abstract
The diffusion coefficients of 14 n-alkanes (ranging from methane to n-tetradecane) in liquid and supercritical methanol at infinite dilution (at a pressure of 10.5 MPa and at temperatures of 299 K and 515 K) were deduced via molecular dynamics simulations. Values for the radial distribution function, coordination number, and number of hydrogen bonds were then calculated to explore the local structure of each fluid. The flexibility of the n-alkane (as characterized by the computed dihedral distribution, end-to-end distance, and radius of gyration) was found to be a major influence and hydrogen bonding to be a minor influence on the local structure. Hydrogen bonding reduces the flexibility of the n-alkane, whereas increasing the temperature enhances its flexibility, with temperature having a greater effect than hydrogen bonding on flexibility. Graphical abstract The flexibility of the alkane is a major influence and the hydrogen bonding is a minor influence on the first solvation shell; the coordination numbers of long-chain n-alkanes in the first solvation shell are rather low.
- Published
- 2017
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6. The characterization of 1-(4-bromophenyl)-5-phenyl-1H-1,2,3-triazole on acute toxicity, antimicrobial activities, photophysical property, and binding to two globular proteins.
- Author
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Liu H, Lin Q, Xie Y, Shu H, Li B, Gao G, Xiao K, Yao X, Dong R, Liu Y, He M, Wu L, Sun Z, and He W
- Subjects
- Animals, Anti-Infective Agents metabolism, Cattle, Microbial Sensitivity Tests, Molecular Docking Simulation, Protein Binding, Triazoles metabolism, Triazoles pharmacology, Anti-Infective Agents pharmacology, Anti-Infective Agents toxicity, Triazoles toxicity
- Abstract
1-(4-Bromophenyl)-5-phenyl-1H-1,2,3-triazole (BPT) was a newly synthesized compound. The acute toxicities of BPT to mice by intragastric administration have been determined and the result indicates that the intragastric administration of BPT did not produce any significant toxic effect on Kunming strain mice. It is also evaluated for the antimicrobial activity of BPT against three kinds of plant mycoplasma, Fusarium Wilt (race 4), Colletotrichum gloeosporioides Penz. and Xanthomonas oryzae by different method in vitro. The compound exhibited distinct inhibitory activities against Fusarium Wilt (race 4) and Colletotrichum gloeosporioides Penz. by mycelium growth rate test and the values of EC
50 were 29.34 and 12.53μg/mL respectively. And BPT had also the most potent inhibitory activities against Xanthomonas oryzae when compared with that of control drugs by the agar well diffusion method. In addition, the structural and photophysical properties of BPT including ionization energy, electron affinities, and theoretical spectrum was studied by quantum-chemical methods. Then the interaction of BPT with two kinds of globular proteins, human immunoglobulin (HIg) and bovine hemoglobin (BHg) was investigated by using UV-vis absorption spectra, synchronous fluorescence, 3D fluorescence spectra, and fluorescence titration in combination with molecular modeling. UV-vis absorption, 3D and synchronous fluorescence measurements show that BPT has influence on the microenvironment surrounding HIg or BHg in aqueous solution and the fluorescence experiments show that BPT quenches the fluorescence intensity of HIg or BHg through a static mechanism. The binding parameters including the binding constants, the number of binding site and average binding distance between BPT and HIg or BHg at different temperatures were calculated. The thermodynamic parameters suggest that the hydrophobic interaction is the predominant intermolecular forces in stabilizing the BPT-HIg or BPT-BHg complex. Molecular docking was performed to reveal that the BPT moiety binds to the hydrophobic cavity of HIg or BHg and they are in good agreement with the spectroscopic measurements., (Copyright © 2016 Elsevier B.V. All rights reserved.)- Published
- 2016
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7. Electrochemical deoxyribonucleic acid biosensor based on electrodeposited graphene and nickel oxide nanoparticle modified electrode for the detection of salmonella enteritidis gene sequence.
- Author
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Sun W, Wang X, Lu Y, Gong S, Qi X, Lei B, Sun Z, and Li G
- Subjects
- Genes, Bacterial, Microscopy, Electron, Scanning, Biosensing Techniques, DNA analysis, Electrochemical Techniques methods, Graphite chemistry, Metal Nanoparticles, Nickel chemistry, Salmonella enteritidis genetics
- Abstract
In this paper a new electrochemical DNA biosensor was prepared by using graphene (GR) and nickel oxide (NiO) nanocomposite modified carbon ionic liquid electrode (CILE) as the substrate electrode. GR and NiO nanoparticles were electrodeposited on the CILE surface step-by-step to get the nanocomposite. Due to the strong affinity of NiO with phosphate groups of ssDNA, oligonucleotide probe with a terminal 5'-phosphate group could be attached on the surface of NiO/GR/CILE, which could further hybridize with the target ssDNA sequence. Methylene blue (MB) was used as the electrochemical indicator for monitoring the hybridization reaction. Under the optimal conditions the reduction peak current of MB was proportional to the concentration of salmonella enteritidis gene sequence in the range from 1.0×10(-13) to 1.0×10(-6)molL(-1) with a detection limit as 3.12×10(-14)molL(-1). This electrochemical DNA sensor exhibited good discrimination ability to one-base and three-base mismatched ssDNA sequences, and the polymerase chain reaction amplification product of salmonella enteritidis gene sequences were further detected with satisfactory results., (Copyright © 2014 Elsevier B.V. All rights reserved.)
- Published
- 2015
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8. Application of graphene-copper sulfide nanocomposite modified electrode for electrochemistry and electrocatalysis of hemoglobin.
- Author
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Shi F, Zheng W, Wang W, Hou F, Lei B, Sun Z, and Sun W
- Subjects
- Catalysis, Coated Materials, Biocompatible chemical synthesis, Equipment Design, Equipment Failure Analysis, Ionic Liquids chemistry, Nanocomposites ultrastructure, Particle Size, Sulfides chemistry, Conductometry instrumentation, Copper chemistry, Electrodes, Graphite chemistry, Hemoglobins analysis, Hemoglobins chemistry, Nanocomposites chemistry
- Abstract
In this paper a graphene (GR) and copper sulfide (CuS) nanocomposite was synthesized by hydrothermal method and used for the electrode modification with a N-butylpyridinium hexafluorophosphate based carbon ionic liquid electrode (CILE) as the substrate electrode. Hemoglobin (Hb) was immobilized on the modified electrode to get a biocompatible sensing platform. UV-vis absorption spectroscopic results confirmed that Hb retained its native secondary structure in the composite. Direct electron transfer of Hb incorporated into the nanocomposite was investigated with a pair of well-defined redox waves appeared on cyclic voltammogram, indicating the realization of direct electrochemistry of Hb on the modified electrode. The results can be ascribed to the presence of GR-CuS nanocomposite on the electrode surface that facilitates the electron transfer rate between the electroactive center of Hb and the electrode. The Hb modified electrode showed excellent electrocatalytic activity to the reduction of trichloroacetic acid in the concentration range from 3.0 to 64.0 mmol L(-1) with the detection limit of 0.20 mmol L(-1) (3σ). The fabricated biosensor displayed the advantages such as high sensitivity, good reproducibility and long-term stability., (Copyright © 2014 Elsevier B.V. All rights reserved.)
- Published
- 2015
- Full Text
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9. Application of graphene-ionic liquid-chitosan composite-modified carbon molecular wire electrode for the sensitive determination of adenosine-5'-monophosphate.
- Author
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Shi F, Gong S, Xu L, Zhu H, Sun Z, and Sun W
- Subjects
- Adenosine Monophosphate standards, Calibration, Catalysis, Dielectric Spectroscopy, Electrodes, Hydrogen-Ion Concentration, Imidazoles chemistry, Oxidation-Reduction, Surface Properties, Adenosine Monophosphate analysis, Carbon chemistry, Chitosan chemistry, Electrochemical Techniques standards, Graphite chemistry, Ionic Liquids chemistry
- Abstract
In this paper, a graphene (GR) ionic liquid (IL) 1-octyl-3-methylimidazolium hexafluorophosphate and chitosan composite-modified carbon molecular wire electrode (CMWE) was fabricated by a drop-casting method and further applied to the sensitive electrochemical detection of adenosine-5'-monophosphate (AMP). CMWE was prepared with diphenylacetylene (DPA) as the modifier and the binder. The properties of modified electrode were examined by scanning electron microscopy, cyclic voltammetry and electrochemical impedance spectroscopy. Electrochemical behaviors of AMP was carefully investigated with enhanced responses appeared, which was due to the presence of GR-IL composite on the electrode surface with excellent electrocatalytic ability. A well-defined oxidation peak of AMP appeared at 1.314 V and the electrochemical parameters were calculated by electrochemical methods. Under the selected conditions, the oxidation peak current of AMP was proportional to its concentration in the range from 0.01 μM to 80.0 μM with the detection limit as 3.42 nM (3σ) by differential pulse voltammetry. The proposed method exhibited good selectivity and was applied to the detection of vidarabine monophosphate injection samples with satisfactory results., (© 2013.)
- Published
- 2013
- Full Text
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10. Molecular dynamics simulation of diffusion and structure of some n-alkanes in near critical and supercritical carbon dioxide at infinite dilution.
- Author
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Feng H, Gao W, Sun Z, Lei B, Li G, and Chen L
- Abstract
The diffusion coefficients of n-alkanes (from CH4 to C14H30) in near critical and supercritical carbon dioxide at infinite dilution have been studied by molecular dynamics simulation. The simulation results agree well with experiment, which suggests that the simulation method is a powerful tool to obtain diffusion coefficients of solutes in fluids at high pressures. The local structures of such fluids are further investigated by calculating radial distribution functions and coordination numbers. Meanwhile, the dihedral, end-to-end distance and radius of gyration, which are calculated to characterize the flexibility of n-alkanes, are used to reasonably explain the abnormal trends on radial distribution functions and coordination numbers. Moreover, it is found that the flexibility effects on diffusion in pure n-alkanes and infinitely dilute n-alkane/CO2 system are different. The differences in MD simulation results of molecular diffusion in such systems could be qualitatively explained by the flexibility.
- Published
- 2013
- Full Text
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11. Simultaneous electrochemical determination of guanosine and adenosine with graphene-ZrO2 nanocomposite modified carbon ionic liquid electrode.
- Author
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Sun W, Wang X, Sun X, Deng Y, Liu J, Lei B, and Sun Z
- Subjects
- Carbon chemistry, Electrodes, Humans, Nanocomposites ultrastructure, Sensitivity and Specificity, Adenosine urine, Electrochemical Techniques instrumentation, Graphite chemistry, Guanosine urine, Ionic Liquids chemistry, Nanocomposites chemistry, Zirconium chemistry
- Abstract
In this paper an ionic liquid 1-hexylpyridinium hexafluorophosphate based carbon ionic liquid electrode (CILE) was fabricated and used as the basal electrode, which was further modified by graphene (GR) and ZrO2 nanoparticle with chitosan (CTS) film to immobilize the nanocomposite. The modified electrode was denoted as CTS-GR-ZrO2/CILE and further used for the simultaneous detection of adenosine and guanosine. Electrochemical performances of the modified electrode were greatly enhanced due to the presence of GR-ZrO2 nanocomposite, and the direct electro-oxidation behaviors of adenosine and guanosine were carefully investigated. Both adenosine and guanosine exhibited an increase of the oxidation peak currents with the negative shift of the oxidation peak potentials on the modified electrode, which indicated the electrocatalytic activity of GR-ZrO2 nanocomposite on the electrode surface. Electrochemical parameters of adenosine and guanosine on CTS-GR-ZrO2/CILE were calculated respectively, and a new electroanalytical method for the simultaneous determination of adenosine and guanosine was further established with the peak-to-peak separation (ΔEp) as 0.225V. The proposed method was successfully applied to detect adenosine and guanosine in human urine samples with satisfactory results., (Copyright © 2013 Elsevier B.V. All rights reserved.)
- Published
- 2013
- Full Text
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12. Direct electrochemistry with enhanced electrocatalytic activity of hemoglobin in hybrid modified electrodes composed of graphene and multi-walled carbon nanotubes.
- Author
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Sun W, Cao L, Deng Y, Gong S, Shi F, Li G, and Sun Z
- Subjects
- Biosensing Techniques instrumentation, Catalysis, Graphite chemistry, Hydrogen Peroxide chemistry, Limit of Detection, Microscopy, Electron, Scanning, Nanotubes, Carbon, Oxidation-Reduction, Reproducibility of Results, Sodium Nitrite chemistry, Spectrophotometry, Ultraviolet, Spectroscopy, Fourier Transform Infrared, Trichloroacetic Acid analysis, Trichloroacetic Acid chemistry, Electrochemistry instrumentation, Electrochemistry methods, Electrodes, Hemoglobins chemistry
- Abstract
A graphene (GR) and multi-walled carbon nanotubes (MWCNT) hybrid was prepared and modified on a 1-hexylpyridinium hexafluorophosphate based carbon ionic liquid electrode (CILE). Hemoglobin (Hb) was immobilized on GR-MWCNT/CILE surface with Nafion as the film forming material and the modified electrode was denoted as Nafion/Hb-GR-MWCNT/CILE. Spectroscopic results revealed that Hb molecules retained its native structure in the GR-MWCNT hybird. Electrochemical behaviors of Hb were carefully investigated by cyclic voltammetry with a pair of well-defined redox peaks obtained, which indicated that direct electron transfer of Hb was realized in the hybrid modified electrode. The result could be attributed to the synergistic effects of GR-MWCNT hybrid with enlarged surface area and improved conductivity through the formation of a three-dimensional network. Electrochemical parameters of the immobilized Hb on the electrode surface were further calculated with the results of the electron transfer number (n) as 1.03, the charge transfer coefficient (a) as 0.58 and the electron-transfer rate constant (ks) as 0.97 s(-1). The Hb modified electrode showed good electrocatalytic ability toward the reduction of different substrates such as trichloroacetic acid in the concentration range from 0.05 to 38.0 mmol L(-1) with a detection limit of 0.0153 mmol L(-1) (3σ), H2O2 in the concentration range from 0.1 to 516.0 mmol L(-1) with a detection limit of 34.9 nmol/L (3σ) and NaNO2 in the concentration range from 0.5 to 650.0 mmol L(-1) with a detection limit of 0.282 μmol L(-1) (3σ). So the proposed electrode had the potential application in the third-generation electrochemical biosensors without mediator., (Copyright © 2013 Elsevier B.V. All rights reserved.)
- Published
- 2013
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13. Fabrication of graphene-platinum nanocomposite for the direct electrochemistry and electrocatalysis of myoglobin.
- Author
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Sun W, Li L, Lei B, Li T, Ju X, Wang X, Li G, and Sun Z
- Subjects
- Animals, Catalysis, Dielectric Spectroscopy, Electrodes, Fluorocarbon Polymers chemistry, Horses, Hydrogen-Ion Concentration, Ionic Liquids chemistry, Nanocomposites ultrastructure, Reproducibility of Results, Spectrophotometry, Ultraviolet, Spectroscopy, Fourier Transform Infrared, Electrochemistry methods, Graphite chemistry, Myoglobin chemistry, Nanocomposites chemistry, Platinum chemistry
- Abstract
In this paper a platinum (Pt) nanoparticle decorated graphene (GR) nanosheet was synthesized and used for the investigation on direct electrochemistry of myoglobin (Mb). By integrating GR-Pt nanocomposite with Mb on the surface of carbon ionic liquid electrode (CILE), a new electrochemical biosensor was fabricated. UV-Vis absorption and FT-IR spectra indicated that Mb remained its native structure in the nanocomposite film. Electrochemical behaviors of Nafion/Mb-GR-Pt/CILE were investigated with a pair of well-defined redox peak appeared, which indicated that direct electron transfer of Mb was realized on the underlying electrode with the usage of the GR-Pt nanocomposite. The fabricated electrode showed good electrocatalytic activity to the reduction of trichloroacetic acid in the linear range from 0.9 to 9.0 mmol/L with the detection limit as 0.32 mmol/L (3σ), which showed potential application for fabricating novel electrochemical biosensors and bioelectronic devices., (Copyright © 2012 Elsevier B.V. All rights reserved.)
- Published
- 2013
- Full Text
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14. Direct electrochemistry of hemoglobin on graphene and titanium dioxide nanorods composite modified electrode and its electrocatalysis.
- Author
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Sun W, Guo Y, Ju X, Zhang Y, Wang X, and Sun Z
- Subjects
- Catalysis, Chitosan chemistry, Electrochemistry methods, Electrodes, Humans, Ionic Liquids, Nanocomposites chemistry, Nanotubes chemistry, Biosensing Techniques methods, Graphite chemistry, Hemoglobins isolation & purification, Titanium chemistry
- Abstract
A biocompatible sensing platform based on graphene (GR) and titanium dioxide (TiO₂) nanorods for the immobilization of hemoglobin (Hb) was adopted in this paper. The GR-TiO₂-Hb composite-modified carbon ionic liquid electrode was constructed through a simple casting method with Nafion as the film forming material. UV-Vis and FT-IR spectra confirmed that Hb retained its native structure in the composite film. Direct electron transfer of Hb incorporated into the composite was realized with a pair of quasi-reversible redox waves appeared, indicating that the presence of GR-TiO₂ nanocomposite on the electrode surface could facilitate the electron transfer rate between the electroactive center of Hb and the substrate electrode. Hb modified electrode showed excellent electrocatalytic activity to the reduction of trichloroacetic acid in the concentration range from 0.6 to 21.0 mmol L⁻¹. These results indicated that GR-TiO₂ nanocomposite could be a friendly biocompatible interface for immobilizing biomolecules and keeping their native structure. The fabricated biosensor displayed the advantages such as high sensitivity, good reproducibility and long-term stability., (Copyright © 2012 Elsevier B.V. All rights reserved.)
- Published
- 2013
- Full Text
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15. MD simulation of self-diffusion and structure in some n-alkanes over a wide temperature range at high pressures.
- Author
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Feng H, Gao W, Nie J, Wang J, Chen X, Chen L, Liu X, Lüdemann HD, and Sun Z
- Abstract
Self-diffusion and structural properties of n-alkanes have been studied by molecular dynamics simulation in the temperature range between the melting pressure curve and 600 K at pressures up to 300 MPa. The simulated results of lower n-alkanes are in good agreement with the existing experimental data, and support the reliability of results of the simulations of self-diffusion coefficients obtained at the extreme conditions. We predict the self-diffusion coefficients for methane, ethane, propane and n-butane at the similar reduced temperatures and pressures to draw a comparison between them. Then the correlation between self-diffusion and structural properties are further investigated by calculating the coordination numbers. Moreover, we define four distances and their corresponding relative deviations to characterize the flexibility of long-chain n-alkanes. The simulated results show that the self-diffusion of n-alkane molecules is mainly affected by the close packing, and the flexibility has a strong impact on the self-diffusion of longer n-alkane molecules.
- Published
- 2013
- Full Text
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16. Electrochemical deoxyribonucleic acid biosensor based on carboxyl functionalized graphene oxide and poly-L-lysine modified electrode for the detection of tlh gene sequence related to vibrio parahaemolyticus.
- Author
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Sun W, Zhang Y, Ju X, Li G, Gao H, and Sun Z
- Subjects
- Amino Acid Sequence, DNA, Single-Stranded chemistry, Electrodes, Polymerase Chain Reaction, Biosensing Techniques, DNA chemistry, DNA, Single-Stranded genetics, Electrochemical Techniques, Graphite chemistry, Oxides chemistry, Polylysine chemistry, Vibrio parahaemolyticus genetics
- Abstract
A carboxyl functionalized graphene oxide (GO-COOH) and electropolymerized poly-l-lysine (PLLy) modified glassy carbon electrode (GCE) was fabricated and used for the construction of an electrochemical deoxyribonucleic acid (DNA) biosensor. The NH(2) modified probe ssDNA sequences were immobilized on the surface of GO-COOH/PLLy/GCE by covalent linking with the formation of amide bonds, which was stable and further hybridized with the target ssDNA sequence. Differential pulse voltammetry (DPV) was used to monitor the hybridization events with methylene blue as electrochemical indicator, which gave a sensitive reduction peak at -0.287 V (vs. SCE). Under the optimal conditions the reduction peak current was proportional to the concentration of tlh gene sequence in the range from 1.0×10(-12) to 1.0×10(-6) mol L(-1) with a detection limit as 1.69×10(-13) mol L(-1) (3σ). The polymerase chain reaction products of tlh gene from oyster samples were detected with satisfactory results, indicating the potential application of this electrochemical DNA sensor., (Copyright © 2012 Elsevier B.V. All rights reserved.)
- Published
- 2012
- Full Text
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17. Poly(methylene blue) functionalized graphene modified carbon ionic liquid electrode for the electrochemical detection of dopamine.
- Author
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Sun W, Wang Y, Zhang Y, Ju X, Li G, and Sun Z
- Subjects
- Dopamine analysis, Dopamine Agents analysis, Electrodes, Humans, Limit of Detection, Reproducibility of Results, Spectrophotometry, Ultraviolet, Carbon chemistry, Dopamine urine, Dopamine Agents urine, Graphite chemistry, Ionic Liquids chemistry, Methylene Blue chemistry
- Abstract
An ionic liquid 1-butylpyridinium hexafluorophosphate based carbon ionic liquid electrode (CILE) was used as the substrate electrode and a poly(methylene blue) (PMB) functionalized graphene (GR) composite film was co-electrodeposited on CILE surface by cyclic voltammetry. The PMB-GR/CILE exhibited better electrochemical performances with higher conductivity and lower electron transfer resistance. Electrochemical behavior of dopamine (DA) was further investigated by cyclic voltammetry and a pair of well-defined redox peaks appeared with the peak-to-peak separation (ΔE(p)) as 0.058V in 0.1 mol L(-1) pH 6.0 phosphate buffer solution, which proved a fast quasi-reversible electron transfer process on the modified electrode. Electrochemical parameters of DA on PMB-GR/CILE were calculated with the electron transfer number as 1.83, the charge transfer coefficients as 0.70, the apparent heterogeneous electron transfer rate constant as 1.72 s(-1) and the diffusional coefficient (D) as 3.45×10(-4) cm(2) s(-1), respectively. Under the optimal conditions with differential pulse voltammetric measurement, the linear relationship between the oxidation peak current of DA and its concentration was obtained in the range from 0.02 to 800.0 μmol L(-1) with the detection limit as 5.6 nmol L(-1) (3σ). The coexisting substances exhibited no interference and PMB-GR/CILE was applied to the detection of DA injection samples and human urine samples with satisfactory results., (Copyright © 2012 Elsevier B.V. All rights reserved.)
- Published
- 2012
- Full Text
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