1. Complex regulation of mitochondrial signaling by human adenovirus minor capsid protein VI.
- Author
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Schubert E, Mun K, Larsson M, Panagiotou S, Idevall-Hagren O, Svensson C, and Punga T
- Subjects
- Humans, Immunity, Innate, Interferon Regulatory Factor-3 metabolism, Interferon Regulatory Factor-3 genetics, Adenovirus Infections, Human virology, Adenovirus Infections, Human metabolism, Mitochondrial Membranes metabolism, HEK293 Cells, Phosphorylation, Cytosol metabolism, Cytosol virology, Adenoviruses, Human physiology, Adenoviruses, Human genetics, Adenoviruses, Human metabolism, Capsid Proteins metabolism, Capsid Proteins genetics, Mitochondria metabolism, Signal Transduction, DNA, Mitochondrial metabolism, DNA, Mitochondrial genetics, Interferon-beta metabolism, Interferon-beta genetics
- Abstract
The controlled release of mitochondrial content into the cytosol has emerged as one of the key steps in mitochondrial signaling. In particular, the release of mitochondrial DNA (mtDNA) into the cytosol has been shown to activate interferon beta (IFN-β) gene expression to execute the innate immune response. In this report, we show that human adenovirus type 5 (HAdV-C5) infection induces the release of mtDNA into the cytosol. The release of mtDNA is mediated by the viral minor capsid protein VI (pVI), which localizes to mitochondria. The presence of the mitochondrial membrane proteins Bak and Bax are needed for the mtDNA release, whereas the viral E1B-19K protein blocked pVI-mediated mtDNA release. Surprisingly, the pVI-mediated mtDNA release did not increase but inhibited the IFN-β gene expression. Notably, the pVI expression caused mitochondrial leakage of the HSP60 protein. The latter prevented specific phosphorylation of the interferon regulatory factor 3 (IRF3) needed for IFN-β gene expression. Overall, we assign a new mitochondria and IFN-β signaling-modulating function to the HAdV-C5 minor capsid protein VI., Importance: Human adenoviruses (HAdVs) are common pathogens causing various self-limiting diseases, including conjunctivitis and the common cold. HAdVs need to interfere with multiple cellular signaling pathways during the infection to gain control over the host cell. In this study, we identified human adenovirus type 5 (HAdV-C5) minor capsid protein VI as a factor modulating mitochondrial membrane integrity and mitochondrial signaling. We show that pVI-altered mitochondrial signaling impedes the cell's innate immune response, which may benefit HAdV growth. Overall, our study provides new detailed insights into the HAdV-mitochondria interactions and signaling. This knowledge is helpful when developing new anti-viral treatments against pathogenic HAdV infections and improving HAdV-based therapeutics., Competing Interests: The authors declare no conflict of interest.
- Published
- 2024
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