1. MMP mediated type V collagen degradation (C5M) is elevated in ankylosing spondylitis.
- Author
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Veidal SS, Larsen DV, Chen X, Sun S, Zheng Q, Bay-Jensen AC, Leeming DJ, Nawrocki A, Larsen MR, Schett G, and Karsdal MA
- Subjects
- Adolescent, Adult, Aged, Animals, Antibodies, Monoclonal immunology, Antibodies, Monoclonal metabolism, Biomarkers blood, Biomarkers metabolism, Case-Control Studies, Collagen Type VI metabolism, Female, Humans, Male, Mice, Mice, Inbred BALB C, Middle Aged, Peptide Fragments, Prognosis, Proteolysis, Retrospective Studies, Spondylitis, Ankylosing diagnosis, Spondylitis, Ankylosing metabolism, Young Adult, Collagen Type VI blood, Enzyme-Linked Immunosorbent Assay methods, Matrix Metalloproteinase 2 metabolism, Matrix Metalloproteinase 9 metabolism, Spondylitis, Ankylosing pathology
- Abstract
Objectives: Type V collagen has been demonstrated to control fibril formation. The aim of this study was to develop an ELISA capable of detecting a fragment of type V collagen generated by MMP-2/9 and to evaluate the assay as biomarker for ankylosing spondylitis (AS)., Design and Methods: A fragment unique to type V collagen and generated by both MMP-2/9 cleaved at the amino acid position 1317 (C5M) was selected for ELISA development. 40 AS patients and 40 age-matched controls were evaluated., Results: An ELISA detecting C5M with inter- and intra-assay variations of 9.1% and 4.4% was developed. C5M levels were significantly higher in AS patients compared to controls, 229% (p<0.0001). The diagnostic AUC was 83%., Conclusions: This ELISA is the first for detecting type V collagen degradation. AS patients had highly elevated levels of MMP mediated type V collagen degradation. The prognostic and diagnostic values need to be further investigated in additional clinical settings., (Copyright © 2012 The Canadian Society of Clinical Chemists. Published by Elsevier Inc. All rights reserved.)
- Published
- 2012
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