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Your search keyword '"Wu, Huihuang"' showing total 3 results
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3 results on '"Wu, Huihuang"'

1. Development of a Smartphone-Based Fluorescent Immunochromatographic Assay Strip Reader.

Author

Zheng Q, Wu H, Jiang H, Yang J, and Gao Y

Subjects
Aflatoxin B1 analysis, Coloring Agents, Humans, Limit of Detection, Immunoassay, Smartphone
Abstract

Fluorescence immunochromatographic assay (FICA) is a rapid immunoassay technique that has the characteristics of high precision and sensitivity. Although image FICA strip readers have the advantages of high portability and easy operation, the use of high-precision complementary metal oxide semiconductor (CMOS) image sensors leads to an increase in overall cost. Considering the popularity of CMOS image sensors in smartphones and their powerful processing functions, this work developed a smartphone-based FICA strip reader. An optical module suitable for the test strips with different fluorescent markers was designed by replacing the excitation light source and the light filter. An android smartphone was used for image acquisition and image denoising. Then, the test and control lines of the test strip image were recognized by the sliding window algorithm. Finally, the characteristic value of the strip image was calculated. A linear detection range from 10 to 5000 mIU/mL ( R 2 = 0.95) was obtained for human chorionic gonadotrophin with the maximum relative error less than 9.41%, and a linear detection range from 5 to 4000 pg/mL ( R 2 = 0.99) was obtained for aflatoxin B1, with the maximum relative error less than 12.71%. Therefore, the smartphone-based FICA strip reader had high portability, versatility, and accuracy.

Published
2020
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2. Automatic Range Adjustment of the Fluorescence Immunochromatographic Assay Based on Image Processing.

Author

Jiang R, Wu H, Yang J, Jiang H, Du M, Vai M, Pun S, and Gao Y

Subjects
Algorithms, Automation, C-Reactive Protein analysis, Entropy, Fluorescence, Humans, Limit of Detection, Image Processing, Computer-Assisted, Immunoassay methods
Abstract

As an emerging technology, fluorescence immunochromatographic assay (FICA) has the advantages of high sensitivity, strong stability and specificity, which is widely used in the fields of medical testing, food safety and environmental monitoring. The FICA reader based on image processing meets the needs of point-of-care testing because of its simple operation, portability and fast detection speed. However, the image gray level of common image sensors limits the detection range of the FICA reader, and high-precision image sensors are expensive, which is not conducive to the popularization of the instrument. In this paper, FICA strips' image was collected using a common complementary metal oxide semiconductor (CMOS) image sensor and a range adjustment mechanism was established to automatically adjust the exposure time of the CMOS image sensor to achieve the effect of range expansion. The detection sensitivity showed a onefold increase, and the upper detection limit showed a twofold increase after the proposed method was implemented. In addition, in the experiments of linearity and accuracy, the fitting degree ( R 2 ) of the fitted curves both reached 0.999. Therefore, the automatic range adjustment method can obviously improve the detection range of the FICA reader based on image processing.

Published
2019
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3. [Preliminary application of injectable calcium phosphate cement/poly (lactic-co-glycolic acid) microspheres for extraction site preservation].

Author

Mai Y, Wu H, Mai Z, Li X, Huang L, and Liao H

Subjects
Animals, Dogs, Glycolates, Lactic Acid, Minerals, Models, Dental, Polyesters, Polyglycolic Acid, Polylactic Acid-Polyglycolic Acid Copolymer, Polymers, Tooth Extraction, Calcium Phosphates, Dental Cements, Microspheres
Abstract

Objective: To investigate the feasibility of extraction site preservation using injectable calcium phosphate cement (CPC) combine with poly (lactic-co-glycolic acid) (PLGA) microspheres., Methods: Immediate extraction defects models were created in canine mandibles, and the defects were filled with CPC/PLGA (experimental group, E) , Bio-Oss (positive control, P), non-treatment (blank control, B) respectively. Dogs were sacrificed after 4, 8, 12 weeks post operation. Statistical analysis were conducted using SPSS 19., Results Results: of radiological observation showed that there were not significantly different between groups in 4 and 8 week (P > 0.05). After 12 week,E (114.9 ± 8.4) were not significantly different compared with P (117.4 ± 12.1) (P > 0.05) , both were significantly higher than B (95.0 ± 12.6) (P < 0.05) . Histology examination showed that at 4 week following surgery, the result of newly formed bone was as follow, P[ (87.5 ± 1.5) %] > B[(78.7 ± 2.7)%] > E[(69.2 ± 1.8)%] (P < 0.05). At 8, 12 week, results of P[(94.0 ± 2.3)% and (93.5 ± 1.9) %] and E[ (94.7 ± 1.1) % and (96.0 ± 0.9) %] were better than those of B[ (76.8 ± 3.0)% and (87.0 ± 2.4)%] (P < 0.05)., Conclusions: The effect of CPC/PLGA repair immediate alveolar ridge defects is the same as that of Bio-Oss, and CPC/PLGA can be used as a material in extraction site preservation.

Published
2014

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