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1. Construction and characterization of a novel maltose inducible expression vector in Bacillus subtilis.

Author

Ming-Ming Y, Wei-Wei Z, Xi-Feng Z, and Pei-Lin C

Subjects
Bacillus subtilis metabolism, Bacterial Proteins metabolism, Enzyme Induction, Genetic Vectors biosynthesis, Hemeproteins metabolism, Maltose metabolism, Promoter Regions, Genetic, Transaminases metabolism, Transformation, Bacterial, beta-Galactosidase metabolism, Bacillus subtilis genetics, Gene Expression Regulation, Bacterial genetics, Genetic Vectors genetics, Operon genetics
Abstract

A maltose-inducible expression vector in Bacillus subtilis has been developed and characterized. The vector permitted beta-galactosidase expression at a high level (maximum activity, 8.16 U/ml) when induced and its expression was markedly repressed by glucose. Using this vector, we successfully expressed the other two genes, bioA and vgb. This thus provided a potential expression system for cloned genes in B. subtilis.

Published
2006
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