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Purpose: Rapid sintering technology has become one of the most direct methods for shortening the manufacturing time of zirconia restorations. This study aimed to explore the aging resistance of rapid-sintered 5 mol% yttria-partially-stabilized zirconia (5Y-PSZ)., Methods: Specimens were made from two types of 5Y-PSZ material and subjected to rapid sintering (RS) and conventional sintering (CS). After in vitro aging for 5 h, morphology observation, grain size measurement, and phase composition analysis were performed. The mechanical properties were evaluated by biaxial, three-point flexural tests, and the Vickers microhardness test. Results were analyzed by 3-way ANOVA., Results: Both the RS group and the CS group had a dense microstructure. The tested zirconia ceramics had different grain sizes, which were affected by the interaction between the sintering method and aging. Both groups revealed the same characteristic peaks of the cubic phase after aging. Regardless of the sintering method used, there was no significant difference in the mechanical properties of the tested zirconia before and after aging., Conclusion: The rapid-sintered 5Y-PSZ materials had a microstructure, phase composition and mechanical properties similar to those of conventional sintered materials. The characteristics of the materials prepared using the two sintering methods did not change significantly after aging.

Cordycepin, a bioactive compound extracted from Cordyceps sinensis , can induce apoptosis in human OEC-M1 oral cancer cells. However, the exact mechanism is still unclear. The present study aimed to investigate the underlying mechanism of cordycepin-induced apoptosis in OEC-M1 cells. Following treatment with cordycepin, apoptosis was examined and quantified using a DNA laddering assay and a cytokeratin 18 fragment enzyme-linked immunosorbent assay, respectively. Expressions of mitogen-activated protein kinases (MAPKs) and apoptosis-related proteins were detected by the western blot analysis. Our results show that a pan-caspase inhibitor, Z-VAD-FMK, could significantly inhibit cordycepin-induced apoptosis in OEC-M1 cells. In addition, treatment with cordycepin not only activated caspase-8, caspase-9, and caspase-3 but also induced Bid and poly ADP-ribose polymerase cleavages. Furthermore, cordycepin also induced the activation of c-Jun N-terminal kinase (JNK), extracellular signal-regulated kinase, and p38 MAPKs. Among MAPKs, activation of JNK solely contributed to cordycepin-induced apoptosis with the activation of caspase-8, caspase-9, and caspase-3 and cleavage of PARP. Taken together, the present study demonstrated that cordycepin activated JNK and caspase pathways to induce apoptosis in OEC-M1 cells., Competing Interests: The authors declare that they have no conflicts of interest., (Copyright © 2022 Kuo-Lung Tung et al.)

Background/purpose: The bond strength and durability of highly translucent zirconia ceramics to dentin is still unclear. The purpose of this study was to investigate the effect of various surface treatments on the bond strength of self-adhesive resin cements to high-translucent zirconia crowns and dentin., Materials and Methods: A high-transparent zirconia and three self-adhesive resin cements (G-CEM LinkAce (GCL), RelyX U200 (RXU) and TotalCem (TTC)) were used. The zirconia surface was sandblasted with 50 μm alumina particles or coated with an SR Link primer, while a dentin primer (Tetric N-Bond Universal, TBU) was applied to the surface of the dentin. By using three self-adhesive resin cements, zirconia samples were bonded to the dentin surfaces of human teeth. The shear strength of the specimens was measured before and after 10,000-cycle thermocycling or 90-day aging., Results: When using GCL to bond with the untreated dentin and various zirconia surfaces, the shear bond strength of the sandblasted (Z SB ) and RS Link primer-coated (Z LK ) groups was significantly higher than that of the untreated control group (Zc). However, in the case of TBU-treated dentin, the shear strength of the Z SB + LK  + D TBU group was significantly higher than that of the other groups. After thermocycling and aging, the shear strength of the Z SB + LK  + D TBU group using GCL and RXU cements decreased slightly, while the TTC showed no impact., Conclusion: The zirconia surface pretreated by sandblasting and bonding agent, which was sequentially bonded with a primer-treated dentin by using resin cements, can provide excellent shear bond strength and anti-aging performance., Competing Interests: The authors have no conflicts of interest relevant to this article., (© 2021 Association for Dental Sciences of the Republic of China. Publishing services by Elsevier B.V.)

Background/purpose: The efficient rapid sintering technique has employed to dental zirconia ceramics for shortening the fabrication time of zirconia restorations. The purpose was to compare the optical properties of two generations of rapid sintered translucent zirconia using two dental colorimeters., Materials and Methods: Two generations of translucent zirconia ceramics, 3 mol% yttria-tetragonal zirconia polycrystal (3Y-TZP): Copran Zr-i Ultra-T (UT) and Cercon HT (HT), and 5 mol% yttria-tetragonal zirconia polycrystal (5Y-TZP): Cercon xt (XT), of different thicknesses (0.5, 0.8, and 1.2 mm; n = 5) underwent rapid sintering (RS) or conventional sintering (CS). The CIELAB values were measured on the white and black backgrounds, respectively, by digital colorimeters, shadepilot, DeguDent (DD) and Easyshade V, Vita (Vita). Translucency parameter (TP), color difference (ΔE), surface morphology, and surface roughness were evaluated., Results: RS resulted in reduced lightness, except in the XT group. The chromaticity increased slightly after RS. Translucency decreased with increasing material thickness. ΔE values differed between both sintering processes but were clinically acceptable (ΔE < 5). Grain size of XT decreased after RS. RS did not affect the surface roughness., Conclusion: RS is a feasible method for shortening the manufacturing time of zirconia restorations. A significant difference in TP value was only in the XT group between both sintering methods as measured on DD. Color differences in rapid sintered translucent zirconia materials are imperceptible and acceptable. The specimen thickness affected more in the TP values of Vita than DD. DD has higher sensitivity to translucency and color compared with Vita., (© 2021 Association for Dental Sciences of the Republic of China. Publishing services by Elsevier B.V.)

Since a portion of patients with nasopharyngeal carcinoma (NPC) do not benefit much from current standard treatments, it is still needed to discover new therapeutic drugs to improve the prognosis of the patients. Considering that Chinese traditional medicine plays a role in inhibiting tumor progression, in this study, we aimed to investigate whether a Chinese herbal formula, Qing Yan Li Ge Tang (QYLGT), has the anticancer activity in NPC cells and explore the underlying mechanism as well. MTT assay, colony formation assay, immunoblotting assay, and DNA laddering assay were performed to assess cell viability, cell colony formation, protein expression, and DNA fragmentation, respectively. Results show that QYLGT was able to inhibit the cell viability and decrease colony formation ability in NPC cells. QYLGT could also increase the formation of intracellular vacuoles and induce the autophagy-related protein expressions, including Atg3, Atg6, and Atg12-Atg5 conjugate in NPC cells. Treatment with an autophagy inhibitor, 3-methyladenine, could significantly recover QYLGT-inhibited cell viability of NPC cells. In addition, QYLGT did not significantly induce apoptosis in NPC cells. We also found that QYLGT had the ability to activate phosphoinositide 3-kinase (PI3K)/Akt/mammalian target of the rapamycin (mTOR) pathway. Treatment with PI3K inhibitors, LY294002 and wortmannin, or mTOR inhibitors, rapamycin and Torin 1, could not only recover QYLGT-inhibited cell viability of NPC cells but also inhibit Atg3 expression. Taken together, our results demonstrated that QYLGT could induce autophagic cell death in NPC cells through the PI3K/Akt/mTOR pathway., Competing Interests: The authors declare that there are no conflicts of interest., (Copyright © 2021 Ching-Huey Yang et al.)

Transition metal dichalcogenides (TMDCs) have recently attracted a tremendous amount of attention owing to their superior optical and electrical properties as well as the interesting and various nanostructures that are created by different synthesis processes. However, the atomic thickness of TMDCs limits the light absorption and results in the weak performance of optoelectronic devices, such as photodetectors. Here, we demonstrate the approach to increase the surface area of TMDCs by a one-step synthesis process of TMDC nanowalls from WO x into three-dimensional (3D) WS 2 nanowalls. By utilizing a rapid heating and rapid cooling process, the formation of 3D nanowalls with a height of approximately 150 nm standing perpendicularly on top of the substrate can be achieved. The combination of core-shell colloidal quantum dots (QDs) with three different emission wavelengths and 3D WS 2 nanowalls further improves the performance of WS 2 -based photodetector devices, including a photocurrent enhancement of 320-470% and shorter response time. The significant results of the core-shell QD-WS 2 hybrid devices can be contributed by the high nonradiative energy transfer efficiency between core-shell QDs and the nanostructured material, which is caused by the spectral overlap between the emission of core-shell QDs and the absorption of WS 2 . Besides, outstanding NO 2 gas-sensing performance of core-shell QDs/WS 2 devices can be achieved with an extremely low detection limit of 50 ppb and a fast response time of 26.8 s because of local p - n junctions generated by p - type 3D WS 2 nanowalls and n - type core-shell CdSe-ZnS QDs. Our work successfully reveals the energy transfer phenomenon in core-shell QD-WS 2 hybrid devices and shows great potential in commercial multifunctional sensing applications.

Utilization of light to boost the performance of gas sensors allows us to operate sensor devices at room temperature. Here, we, for the first time, demonstrated an indoor light-activated 3D cone-shaped MoS2 bilayer-based NO gas sensor with ppb-level detection operated at room-temperature. Large-area cone-shaped (CS)-MoS2 bilayers were grown by depositing 2 nm-thick MoO3 layers on a 2'' three-dimensional (3D) cone-patterned sapphire substrate (CPSS) followed by a sulfurization process via chemical vapor deposition. Because the exposed area of MoS2 bilayers is increased by 30%, the CS-MoS2 gas sensor (GS) demonstrated excellent performance with a response of ∼470% and a fast response time of ∼25 s after exposure to 1 ppm of NO gas illuminated by ultraviolet (UV) light with a wavelength of 365 nm. Such extraordinary performance at room temperature is attributed to the enhanced light absorption because of the light scattering effect caused by the 3D configuration and photo-desorption induced by UV illumination. For NO concentrations ranging from 2 ppm down to 0.06 ppm, the CS-MoS2 GS demonstrated a stable sensing behavior with a high response and fast response time (470% and 25 s at 2 ppm NO) because of the light absorption enhanced by the 3D structure and photo-desorption under constant UV illumination. The CS-MoS2 GS exhibits a high sensitivity (∼189.2 R% ppm-1), allowing the detection of NO gas at 0.06 ppm in 130 s. In addition, the 3D cone-shaped structure prolonged the presence of sulfur vapor around MoO3, allowing MoO3 to react with sulfur completely. Furthermore, the CS-MoS2 GS using an indoor lighting to detect NO gas at room temperature was demonstrated for the first time where the CS-MoS2 GS exhibits a stable cycling behavior with a high response (165% at 1 ppm NO) in 50 s; for concentration as low as ∼0.06 ppm, the response of ∼75% in 150 s can be achieved.

Statement of Problem: The press-on-metal (PoM) technique has been used as an alternative fabrication method for metal-ceramic restorations. However, how the PoM technique compares with the conventional porcelain layering (CPL) technique under a variety of conditions is unclear., Purpose: The purpose of this in vitro study was to compare the bond strength of 3 alloy substrates with heat-pressed ceramics or conventionally layered porcelain before and after thermocycling., Material and Methods: Specimens (n=5) of Au, Pd, and Ni-Cr alloys were veneered with heat-pressed ceramics or conventionally layered porcelain. The 3-point bend test was conducted according to the International Organization for Standardization standard 9693-1 as bond strength before and after thermocycling. The metal-ceramic interfaces were characterized by field emission scanning electron microscopy (FESEM) and energy dispersive X-ray spectroscopy (EDS). Two- and 3-way ANOVA followed by the Tukey honestly significant difference (HSD) test were used to analyze the data (α=.05)., Results: Significantly lower mean bond strength was recorded for the Au and Pd alloys of the PoM group than for those of the CPL group (P<.05). CPL-Au demonstrated the highest bond strength of 50.2 ±2.0 MPa, whereas PoM-Pd showed the lowest bond strength of 31.8 ±2.7 MPa; significant differences were found among all groups (P<.05). After 20 000 thermocycles, CPL-Au showed significantly reduced bond strength value (P<.05). A value of approximately 40 MPa was observed in all groups except for PoM-Pd (26.5 ±1.6 MPa, P<.05). The metal-ceramic interface resulting from the PoM technique revealed 2- to 20-μm pores, with more defects observed in the PoM-Pd group than in any of the other group., Conclusions: Defects and an oxide layer were formed at the metal-ceramic interface during the heat-pressing process, especially for the Pd alloy. After thermocycling, PoM-Pd had the lowest bond strength value, although it exceeded the minimum 25 MPa of the ISO 9693-1 standard. The Au and Ni-Cr alloys exhibited similar levels of porcelain bond strength with both techniques., (Copyright © 2019 Editorial Council for the Journal of Prosthetic Dentistry. Published by Elsevier Inc. All rights reserved.)

Background/purpose: With the technology of dental prostheses and materials progress, the bond durability of the all-ceramic restoration system plays an important role in the oral environment. The purpose of this study was to evaluate the influence of the parameters of blasting on the shear bond strength between zirconia and pressed veneer ceramics., Materials and Methods: Zirconia was blasted with different alumina particle size subjected to two types of applied pressures. Heat-pressed and layered veneer ceramic blocks were served as an experimental group and control group, respectively. The shear strength of specimens after thermocycling for 20,000 times was also investigated to simulate oral environment., Results: The results indicated that the surface roughness was increased significantly (P < 0.05) with increasing particle size of alumina and blasting pressure. The alumina particle size had statistically significant influence (P < 0.05) on shear strength of heat-pressed groups. Among heat-pressed ceramic specimens, the highest and lowest shear strength could be obtained when 50 μm of alumina was used at pressure of 0.3 MPa and 110 μm of alumina was used at 0.5 MPa, respectively. The negligible effect of thermal cycle on shear strength of heat-pressed groups can be seen., Conclusion: Blasting with 50 μm of alumina at 0.3 MPa could enhance the bond strength between zirconia and veneer ceramics.

Objective: Commercial quick-heating phosphate-bonded investments are frequently used to reduce the operating time during the dental casting process. The aim of this study was to evaluate the characteristics of commercial quick-heating phosphate-bonded investments., Method and Materials: Characterization of the 2 investments (Maruvest-Speed [Megadental] and Z4 C&B [Neirynck and Vogt]) focused on the setting expansion, compressive strength, thermal expansion, and surface roughness. Two different heating methods-the quick-heating method (QHM) and conventional heating method (CHM)-were used with the investments. The dimensional accuracy and surface roughness of the nickel-chromium alloy castings obtained from the investments were also examined., Results: The Maruvest-Speed investment had a significantly greater setting expansion (2.2%) than the Z4 investment (1.1%) after a 30-minute setting time. The fired strength of both investments was greater with QHM (21.2 to 27.7 MPa) than with CHM (13.8 to 17.9 MPa). The thermal expansion of the Maruvest-Speed investment and Z4 with QHM was 1.7% and 1.4%, respectively. There was no significant difference in surface roughness of the castings between samples treated with QHM and CHM. The dimensional accuracy of the castings was larger in length and slightly deformed in bend., Conclusion: The characteristics of the 2 commercial quick-heating phosphate-bonded investments for the accelerated casting technique may be acceptable.