1. Laccase stabilization by covalent binding immobilization on activated polyvinyl alcohol carrier.
- Author
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Yinghui D, Qiuling W, and Shiyu F
- Subjects
- Chlorophenols metabolism, Enzyme Stability, Industrial Waste, Laccase, Paper, Waste Disposal, Fluid methods, Basidiomycota enzymology, Enzymes, Immobilized, Oxidoreductases chemistry, Oxidoreductases metabolism, Polyvinyl Alcohol chemistry
- Abstract
Aims: Attempts were made to immobilize laccase from Panus conchatus., Methods and Results: The laccase was immobilized on carboxylated polyvinyl alcohol (PVA) activated by N-hydroxysuccinimide (N-HSI) in aqueous solution at different pHs, temperatures, and with different reaction times. An optimum condition for laccase immobilization is at pH 3.2, 40 degrees C and 12 h, respectively. Immobilization of laccase increased optimal pH for reaction with 2, 2'-azinobis (3-ethylbenzthiazoline-6-solfonate) (ABTS) and pH stability. Immobilized laccase proved to be reacted consecutively 17 times with only a 50% decrease on activity and be used in removal of 2,4,6-trichlorophenol (TCP)., Conclusions: It was possible to immobilize the laccase on carboxylated polyvinyl alcohol by activation with N-hydroxysuccinimide in HAc-NaAc buffer. The immobilized laccase is both stable and reusable. SIGNIFICANCE IMPACT OF THE STUDY: The results indicate that this immobilized laccase can be used in the removal of poisonous effluent from pulp bleaching mills.
- Published
- 2002
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