1. Winogradskyella pelagia sp. nov., isolated from coastal sediment.
- Author
-
Li F, Sun YY, Zhang YJ, Liu XJ, Du ZJ, and Gong Y
- Subjects
- China, Seawater microbiology, Molecular Sequence Data, Phosphatidylethanolamines, RNA, Ribosomal, 16S genetics, Phylogeny, Geologic Sediments microbiology, Base Composition, Fatty Acids chemistry, DNA, Bacterial genetics, Bacterial Typing Techniques, Vitamin K 2 analogs & derivatives, Vitamin K 2 analysis, Nucleic Acid Hybridization, Sequence Analysis, DNA
- Abstract
An orange-pigmented, Gram-stain-negative, strictly aerobic, non-flagellated and rod-shaped bacterium, designated strain DF17
T , was isolated from coastal sediment collected from Jingzi Wharf, Weihai, PR China. The optimal growth conditions were determined to be at 30 °C, pH 7.5, and in 3 % (w/v) NaCl. According to phylogenetic analysis of the 16S rRNA gene sequence, strain DF17T showed the highest sequence similarity of 96.9 % to Winogradskyella aquimaris KCTC 23502T . The DNA G+C content was 35.8 mol%, and the major fatty acids were iso-C15 : 1 G, iso-C15 : 0 , and iso-C17 : 0 3-OH. The major polar lipids were two aminoglycolipids, one phosphatidylethanolamine and four unidentified lipids. The predominant respiratory quinone was menaquinone-6 (MK-6). The average nucleotide identity, digital DNA-DNA hybridization, and amino acid identity values between strain DF17T and other Winogradskyella species were below the species delineation thresholds of 69.35-72.95 %, 16.9-19.6 % and 71.25-78.93 %, respectively. On the basis of its phenotypic, genetic and physiological characteristics, strain DF17T is suggested to represent a novel species of the genus Winogradskyella , for which the name Winogradskyella pelagia sp. nov. is proposed. The type strain is DF17T (MCCC 1H00456T =KCTC 82421T ).- Published
- 2024
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