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Ethnopharmacological Relevance: Tianshu capsule (TSC) is a traditional Chinese medicine (TCM) preparation and has significant clinical effect on migraine. The composition characteristics of TSC formula are worth exploring for the treatment of migraine., Aim of the Study: Identify the compounds in vivo after oral administration of TSC, Gastrodin (GAS), ferulic acid, senkyunolide G and senkyunolide I in the blood of healthy and migraine rats were used as representative compounds for time-dependent processes investigation. And we focus on explaining the characteristics of TSC treatment on migraine from a pharmacokinetic perspective., Materials and Methods: In this study, Ultra-high performance liquid chromatography with Orbitrap tandem mass spectrometry (UPLC-Orbitrap-MS/MS) system were used to detect the compounds in rat plasma and brain after oral administration of TSC. A sensitive, selective and reliable ultra-performance liquid chromatography coupled with triple quadrupole mass spectrometry (UPLC-TQ-MS/MS) system method was established for the simultaneous quantitative analysis of multi-components and evaluate the pharmacokinetic behavior of four main compounds., Results: A total of 46 compounds were significantly identified in vivo of rat, including 35 compounds in plasma, 7 compounds only in brain and 4 compounds both in plasma and brain. The quantitative method has been confirmed to be feasible for multi-components content determination, pharmacokinetic parameters indicated that the four compounds absorbed into blood rapidly, and senkyunolide I also cross the blood-brain barrier (BBB) quickly into brain. GAS has relatively high concentrations in plasma, and the parameters AUC 0-t , CL z/F displayed significant differences between the normal and migraine rats. And the AUC 0-t and C max of GAS and ferulic acid exhibited dose-dependent way., Conclusions: we added compounds for the pharmacokinetic study of TSC, providing powerful help for clinical medication in the treatment of migraine., Competing Interests: Declaration of competing interest The authors confirm that there are no conflicts of interest., (Copyright © 2024. Published by Elsevier B.V.)

Preliminary investigations have demonstrated that the cysteines located at the C-terminus of HEV ORF2 protein exhibits disulfide bonding capability during virus-like particles (VLPs) assembly. However, the effect and mechanism underlying the pairing of disulfide bonds formed by C627, C630, and C638 remains unclear. The p222 protein encompasses C-terminus and serves as a representative of HEV ORF2 to investigate the specific impacts of C627, C630, and C638. The three cysteines were subjected to site-directed mutagenesis and expressed in prokaryotes; Both the mutated proteins and p222 underwent polymerization except for p222A; Surprisingly, only p222 was observed as abundant spherical particles under transmission electron microscope (TEM); Stability and immunogenicity of the p222 exhibited higher than other mutated proteins; LC/MS/MS analysis identified four disulfide bonds in the p222. The novel findings suggest that the three cysteines contribute to structural and functional properties of ORF2 protein, highlighting the indispensability of each cysteine., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier Inc. All rights reserved.)

A novel Gram-positive strain, B1 T , was isolated from uranium-contaminated soil. The strain was aerobic, rod-shaped, spore-forming, and motile. The strain was able to grow at 20-45 °C, at pH 6.0-9.0, and in the presence of 0-3 % (w/v) NaCl. The complete genome size of the novel strain was 3 853 322 bp. The genomic DNA G+C content was 45.5 mol%. Phylogenetic analysis based on the 16S rRNA gene sequence showed that strain B1 T has the highest similarity to Aneurinibacillus soli CB4 T (96. 71 %). However, the novel strain showed an average nucleotide identity value of 89.02 % and a digital DNA-DNA hybridization value of 37.40 % with strain CB4 T based on the genome sequences. The major fatty acids were iso-C 15 : 0 and C 16 : 0 . The predominate respiratory quinone was MK7. Diphosphatidylglycerol, phosphatidylmethylethanolamine, phosphatidylethanolamine, phosphatidylglycerol, unidentified phospholipids, an unidentified aminolipid and an unidentified lipid were identified as the major polar lipids. The phylogenetic, phenotypic, and chemotaxonomic analyses showed that strain B1 T represents a novel species of the genus Aneurinibacillus , for which the name Aneurinibacillus uraniidurans sp. nov. is proposed. The type strain is B1 T (=GDMCC 1.4080 T =JCM 36228 T ). Experiments have shown that strain B1 T demonstrates uranium tolerance.

We employ the eigen microstates approach to explore the self-organized criticality (SOC) in two celebrated sandpile models, namely the BTW model and the Manna model. In both models, phase transitions from the absorbing state to the critical state can be understood by the emergence of dominant eigen microstates with significantly increased weights. Spatial eigen microstates of avalanches can be uniformly characterized by a linear system size rescaling. The first temporal eigen microstates reveal scaling relations in both models. Furthermore, by finite-size scaling analysis of the first eigen microstates, we numerically estimate critical exponents, i.e., sqrt[σ_{0}w_{1}]/v[over ̃]_{1}∝L^{D} and v[over ̃]_{1}∝L^{D(1-τ_{s})/2}. Our findings could provide profound insights into eigen microstates of the universality and phase transition in nonequilibrium complex systems governed by self-organized criticality.

To assess the effect of the plate model on the remission of diabetes and the demand for hypoglycemic drugs in China. We selected 456 participants with newly diagnosed type 2 diabetes and not required to take hypoglycemic drugs at baseline. The plate education model consists of three parts: a colored leaflet suitable for low literacy reading, regular medical visits and health education sessions. The primary outcomes were remission of diabetes and the time to first use of hypoglycemic drugs. The study was ended after 8.1 years of follow-up. The incidence of the using hypoglycemic drugs was 36.15 % in the plate model, and 75.54 % in the low-fat model ( P  < 0.001). The prevalence of any remission in plate model was 27.1 % (95 % CI 16.8-37.4 %) during the first 2 years, decreasing to 14.5 % (95 % CI 6.3-22.7 %) during year 4, to 10.1 % (95 % CI 4.4-15.8 %) during year 6, and to 9.6 % (95 % CI 5.3-13.9 %) during year 8, compared with 12.2 % (95 % CI 5.2-19.2 %) at year 2, 6.1 % (95 % CI 2.1-10.1 %) at year 4, 4.7 %(95 % CI 2.2-7.2 %) at year 6, and 2.6 % (95 % CI 1.1-4.2 %) at year 8 in the low-fat group. The HbA1c of plate group was significantly decreased at the endpoint (7.74 ± 0.45 % vs. 6.70 ± 0.46 %, P  < 0.001). The plate model may significantly improve the remission rate of diabetes, delay the demand for diabetes drugs, more suitable for patients with low educational level, and reduce the long-term level of HbA1c., Clinical Trials Registry: The study was registered at ChiCTR (www.chictr.org.cn) (ChiCTR1900027097)., Competing Interests: The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (© 2023 The Author(s).)

Working memory (WM) can maintain sequential and concurrent information, and the load enhances the γ band oscillation during the delay period. To provide a unified account for these phenomena in working memory, we investigated a continuous network model consisting of pyramidal cells, high-threshold fast-spiking interneurons (FS), and low-threshold nonfast-spiking interneurons (nFS) for working memory of sequential and concurrent directional cues. Our model exhibits the γ (30-100 Hz) and β (10-30 Hz) band oscillation during the retention of both concurrent cues and sequential cues. We found that the β oscillation results from the interaction between pyramidal cells and nFS, whereas the γ oscillation emerges from the interaction between pyramidal cells and FS because of the strong excitation elicited by cue presentation, shedding light on the mechanism underlying the enhancement of γ power in many cognitive executions., Competing Interests: The authors declare no competing financial interests., (Copyright © 2023 Zhao et al.)

Over the past decade, the Chinese government has implemented the "Clean Air Action" measures to enhance the atmospheric environmental quality, primarily focusing on curbing PM 2.5 and O 3 concentrations. The efficacy of these strategies and the underlying causes (human factors or natural variability) of any observed increases or decreases in PM 2.5 and O 3 concentrations are of great importance. Examining the hourly PM 2.5 and O 3 concentration time series from six representative regions in China between 2015 and 2021 revealed an overall downward trend in PM 2.5 concentrations. However, the O 3 concentration time series indicated upward trends in some regions, except for the Northeast area (NE) and Sichuan Basin (SCB). In the context of conventional significance tests, the assumption is typically that the time series' samples are independent and therefore memoryless. However, in situations where the time series exhibits strong autocorrelation and limited sample size, this assumption can lead to an overestimation of the statistical significance of the linear trend. To account for this, we utilized a long-term memory model that can reproduce the long-term persistence of pollutant records to improve the accuracy of significance tests. By comparing the P-values of real and surrogate data generated by the long-term memory model, we found that only PM 2.5 concentrations in the Pearl River Delta (PRD) were slightly insignificant. For the remaining five regions, the P-values of PM 2.5 concentrations were smaller than the significant level of 0.05, suggesting that the observed downward trends in PM 2.5 concentrations are not due to natural variability, thereby confirming the effectiveness of the government's policies aimed at curbing atmospheric particulate matter in recent years. Our results show that O 3 pollution is significantly increasing only in the Beijing-Tianjin-Hebei (BTH) region, beyond natural variability. In contrast, the trends of O 3 pollution in many regions of China are markedly impacted by natural and climate variability., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 The Authors. Published by Elsevier B.V. All rights reserved.)

We prospectively analyzed the association between mobile phone usage time and the incidence of diabetic retinopathy (DR) in type 2 diabetes (T2D) among participants.We included a total of 4,371 patients with T2D among the participants. Mobile phone usage time was quantified at baseline by summing up the hours spent on mobile phone use. The types of mobile phone usage time in our study include game time, TikTok time, WeChat time, watching movies or reading time, and online shopping time. We categorized patients into four groups according to different mobile phone usage time: ≤1.5 h/day (n = 1,101), 1.6-3.5 h/day (n = 1,098), 3.6-7.5 h/day (n = 1,095), and >7.6 h/day (n = 1,077). Fundus photography was performed every year from January 2012 to January 2020. During a follow-up of 8 years, 1,119 were affected by DR, resulting in an overall incidence of 25.6%. The incidences of mild nonproliferative DR (NPDR), moderate NPDR, severe NPDR, and proliferative DR (PDR) were 10.1%, 5.1%, 5.1%, and 5.2%, respectively. In comparisons with participants in the lowest category (≤1.5 h/day), the hazard ratios (HRs) of DR were 1.19 (95% confidence interval [CI] 1.07, 1.31, p = 0.040) for 1.6-3.5 h/day, 1.60 (95% CI 1.40, 1.81, p < 0.001) for 3.6-7.5 h/day, and 1.85 (95% CI 1.61, 2.09, p < 0.001) for >7.6 h/day, respectively. Our results provide the general population with a feasible and practical alternative for the reduction of mobile phone use behavior time and new measures to prevent the occurrence of DR.

Background: In view of the complexity of dietary and nutritional education for most patients with type 2 diabetes mellitus (T2DM), a simplified approach called the "restricted diet with a plate" or "plate model" is recommended., Purpose: To evaluate whether the plate model can effectively improve glycemic control and cardiovascular risk markers in type 2 diabetes mellitus (T2DM), while reducing the time devoted to education and avoiding weight gain., Methods: The study was a randomized, multicenter, controlled study, conducted between October 2018 and October 2019, among patients with T2DM living in Nanjing. The study included 419 participants who were randomly divided into a plate group and a counting group. The plate model included three components: a low-literacy, color leaflet containing the explanation and composition of the plate model, health education, and medical visits. Patients in the counting group received health education, group medical visits, and a paper booklet containing traditional carbohydrate counting education. Primary outcomes were glycemic control and weight., Results: Participants in the plate model reduced HbA 1c by 0.7% in the first three months, and reduced to a greater extent at six months (1.44%), but this was not sustained, and HbA 1c increased slightly over the following six months. Fasting plasma glucose (FPG) and 2-h postprandial glucose (2hPG) values were significantly reduced at the endpoint in the plate model (9.25 ± 1.72% vs. 7.44 ± 0.88%, P = 0.008; 12.07 ± 2.94 vs. 8.35 ± 1.99%; P = 0.004); however, the 2hPG values decreased most significantly. Total cholesterol (TC) and low-density lipoprotein cholesterol (LDL-C) levels decreased significantly in the plate group, which occurred at six months and lasted for 12 months. In the first three months, the average weight loss in the plate group was 1.2 kg/month (95% CI 0.92-1.48), and in the fourth to twelfth months, the average weight gain was 0.21 kg/month (95% CI 0.08-0.34). There was significant difference in education time between the groups (17.3 ± 4.42 vs. 38.6 ± 12.63; P < 0.001)., Conclusions: The plate model is at least as effective as the counting model over the short term for glycemic control and perhaps even better for weight and lipid control. Plate model has the potential to improve education of those with low health literacy by reducing reading demands., (Copyright © 2022 The Authors. Published by Elsevier Ltd.. All rights reserved.)

Studies on stratospheric ozone have attracted much attention due to its serious impacts on climate changes and its important role as a tracer of Earth's global circulation. Tropospheric ozone as a main atmospheric pollutant damages human health as well as the growth of vegetation. Yet, there is still a lack of a theoretical framework to fully describe the variation of ozone. To understand ozone's spatiotemporal variance, we introduce the eigen microstate method to analyze the global ozone mass mixing ratio between January 1, 1979 and June 30, 2020 at 37 pressure layers. We find that eigen microstates at different geopotential heights can capture different climate phenomena and modes. Without deseasonalization, the first eigen microstates capture the seasonal effect and reveal that the phase of the intra-annual cycle moves with the geopotential heights. After deseasonalization, by contrast, the collective patterns from the overall trend, El Niño-Southern Oscillation (ENSO), quasi-biennial oscillation, and tropopause pressure are identified by the first few significant eigen microstates. The theoretical framework proposed here can also be applied to other complex Earth systems.

Aim: This study aims to explore the effect of integrated traditional Chinese and Western medicine management mode on nursing care of patients with coronavirus disease 2019 (COVID-19)., Methods: The study included 142 COVID-19 patients hospitalized in Huoshenshan Hospital from February to March 2020. Nursing measures in the treatment (n = 111) and control (n = 31) groups included integrated management care and usual care. The integrated management model had eight components: WeChat group management, early Traditional Chinese Medicine (TCM) treatment, emotional nursing, music therapy, observation of tongue coating and pulse, vital Qi strengthening, preventing recurrence after recovery, and an individualized follow-up scheme. The endpoints included clinical outcome, recovery time of clinical symptoms and signs, days of hospital stay, and survival without intensive care unit (ICU) admission., Results: Recovery time from clinical symptoms such as fever, cough, shortness of breath, muscle aches, and fatigue in the treatment group was shorter than that in the control group. Less time was required to show negative chest computed tomography findings in the treatment group than in the control group (13.0 ± 2.8 vs. 16.7 ± 3.9, p < .025). The lengths of hospital stay in the two groups were 16.4 ± 7.3 days (treatment group) and 24.4 ± 4.8 days (control group) (p < .001). Kaplan-Meier curves for survival without ICU transfer showed statistical difference between the two groups (χ 2 = 5.427, p = .020)., Conclusions: Our study shows that the integrated management mode can relieve the symptoms of patients faster, shorten hospital stay and disease course, and reduce the rate of ICU transfers., (© 2021 Japan Academy of Nursing Science.)

In recent years in China, leaf spot caused by Colletotrichum species has been an emerging disease of Philodendron tatei cv. Congo. From 2016 to 2019, typical symptoms, appearing as circular or ovoid, sunken, and brown lesions with a yellow halo, were commonly observed on P. tatei cv. Congo in and around Lanzhou, Gansu Province, China. Conidiomata were often visible on infected leaf surfaces. Leaf disease incidence was approximately 5 to 20%. A total of 126 single-spored Colletotrichum isolates were obtained from leaf lesions. Multilocus phylogenetic relationships were analyzed based on seven genomic loci (ITS, ACT , GAPDH , HIS3 , CAL , CHS-1 , and TUB2 ) and the morphological characters of the isolates determined. These isolates were identified as three Colletotrichum species in this study. A further 93 isolates, accounting for 74% of all Colletotrichum isolates, were described as new species and named as Colletotrichum philodendricola sp. nov. after the host plant genus name, Philodendron ; another two isolates were named as C. pseudoboninense sp. nov. based on phylogenetic and morphological relativeness to C. boninense ; the other 31 isolates, belonging to the C. orchidearum species complex, were identified as a known species- C. orchidearum . Both novel species C. philodendricola and C. pseudoboninense belong to the C. boninense species complex. Pathogenicity tests by both spray and point inoculations confirmed that all three species could infect leaves of P. tatei cv. Congo. For spray inoculation, the mean infection rate of leaves on the three species was only 4.7% (0 to 12%), and the size on lesions was mostly 1 to 2 mm in length. For point inoculation, 30 days after nonwounding inoculation, the infection rate on leaves was 0 to 35%; in wounding inoculation, the infection rate of leaves was 35 to 65%; wounding in healthy leaves greatly enhanced the pathogenicity of these three species to P. tatei cv. Congo; however, the sizes of lesions among the three species were not significantly different. To our knowledge, this is the first report of Colletotrichum species associated with anthracnose diseases on P. tatei cv. Congo. Results obtained in this study will assist the disease prevention and appropriate management strategies.

This study aimed to systematically review neuropsychiatric lupus erythematosus (NPSLE) and establish a simplified diagnostic criterion for NPSLE. Publications from 1994 to 2018 in the database (Wanfang data (http://www.wanfangdata.com.cn/index.html) and China National Knowledge Internet (http://www.cnki.net)) were included. In total, 284 original case reports and 24 unpublished cases were collected, and clinical parameters were analyzed. An attempt was made to develop a set of simplified diagnostic criteria for NPSLE based on cases described in the survey and literature; moreover, and pathophysiology and management guidelines were studied. The incidence rate of NPSLE was estimated to be 12.4% of SLE patients in China. A total of 408 NPSLE patients had 652 NP events, of which 91.2% affected the central nervous system and 8.8% affected the peripheral nervous system. Five signs (manifestations, disease activity, antibodies, thrombosis, and skin lesions) showed that negative and positive predictive values were more than 70%, included in the diagnostic criteria. The specificity, accuracy, and positive predictive value (PPV) of the revised diagnostic criteria were significantly higher than those of the American College of Rheumatology (ACR) criteria (χ2=13.642, 15.591, 65.010, p<0.001). The area under the curve (AUC) for revised diagnostic criteria was 0.962 (standard error=0.015, 95% confidence intervals [CI] =0.933-0.990), while the AUC for the ACR criteria was 0.900 (standard error=0.024, 95% CI=0.853-0.946). The AUC for the revised diagnostic criteria was different from that for the ACR criteria (Z=2.19, p<0.05). Understanding the pathophysiologic mechanisms leading to NPSLE is essential for the evaluation and design of effective interventions. The set of diagnostic criteria proposed here represents a simplified, reliable, and cost-effective approach used to diagnose NPSLE. The revised diagnostic criteria may improve the accuracy rate for diagnosing NPSLE compared to the ACR criteria.

Background: Health education is considered to be essential in the overall care of patients with type 2 diabetes mellitus (T2DM); systematic health education integrates individual education not only during hospitalization but also extended care outside of a hospital. To test effectiveness of the systematic health education model for T2DM, we conducted a randomized study with a control group among patients with T2DM living in Nanjing, China., Methods: 998 eligible patients completed the enrollment and were randomized to systematic health education model and conventional model groups (498 and 500 patients, resp.). The systematic health education model was based on the following aspects: image education, visitation of the exhibition hall, dissemination of educational materials, individualized medical nutrition therapy and exercise programs, WeChat group and regular health lectures, evaluation of complications, lifestyle modification, systematic treatment scheme, self-monitoring of glycemic control, monthly evaluation of the therapeutic effect, proposed improvement measures, and individualized follow-up scheme. The main outcome measures were glycated hemoglobin A1c (HbA1c), blood pressure, body mass index (BMI), and lipids during the 2-year follow-up., Results: The systematic health education model led to a favorable variation in HbA1c, LDL cholesterol, and systolic blood pressure (SBP) ( P < 0.05). After adjusted analysis, the HbA1c decreased by 0.67% ( P < 0.01) in the systematic health education model, SBP decreased by 10.83 mmHg ( P < 0.01), and the level of diastolic blood pressure (DBP), HDL cholesterol, and total cholesterol decreased slightly and was not significant. The BMI did not change significantly during the study in either of the two groups., Conclusions: The systematic health education model is a useful method in the treatment of T2DM because it contributes to decrease in HbA1c, LDL cholesterol, and SBP levels, as well as helps in increasing the compliance with the control criteria, except for DBP and BMI.

The study is aimed to establish a simplified and practical tool for analyzing thyroid nodules. A novel risk score model was designed, risk factors including patient history, patient characteristics, physical examination, symptoms of compression, thyroid function, ultrasonography (US) of thyroid and cervical lymph nodes were evaluated and classified into high risk factors, intermediate risk factors, and low risk factors. A total of 243 thyroid nodules in 162 patients were assessed with risk score system and Thyroid Imaging-Reporting and Data System (TI-RADS). The diagnostic performance of risk score system and TI-RADS was compared. The accuracy in the diagnosis of thyroid nodules was 89.3% for risk score system, 74.9% for TI-RADS respectively. The specificity, accuracy and positive predictive value (PPV) of risk score system were significantly higher than the TI-RADS system (χ 2 =26.287, 17.151, 11.983; p <0.05), statistically significant differences were not observed in the sensitivity and negative predictive value (NPV) between the risk score system and TI-RADS (χ 2 =1.276, 0.290; p>0.05). The area under the curve (AUC) for risk score diagnosis system was 0.963, standard error 0.014, 95% confidence interval (CI)=0.934-0.991, the AUC for TI-RADS diagnosis system was 0.912 with standard error 0.021, 95% CI=0.871-0.953, the AUC for risk score system was significantly different from that of TI-RADS (Z=2.02; p <0.05). Risk score model is a reliable, simplified and cost-effective diagnostic tool used in diagnosis of thyroid cancer. The higher the score is, the higher the risk of malignancy will be., Competing Interests: The authors declare that they have no conflict of interest., (© Georg Thieme Verlag KG Stuttgart · New York.)

Clique percolation has attracted much attention due to its significance in understanding topological overlap among communities and dynamical instability of structured systems. Rich critical behavior has been observed in clique percolation on Erdős-Rényi (ER) random graphs, but few works have discussed clique percolation on finite dimensional systems. In this paper, we have defined a series of characteristic events, i.e., the historically largest size jumps of the clusters, in the percolating process of adding bonds and developed a new finite-size scaling scheme based on the interval of the characteristic events. Through the finite-size scaling analysis, we have found, interestingly, that, in contrast to the clique percolation on an ER graph where the critical exponents are parameter dependent, the two-dimensional (2D) clique percolation simply shares the same critical exponents with traditional site or bond percolation, independent of the clique percolation parameters. This has been corroborated by bridging two special types of clique percolation to site percolation on 2D lattices. Mechanisms for the difference of the critical behaviors between clique percolation on ER graphs and on 2D lattices are also discussed.

This work is focused on the controlled drug release behavior of hyperbranched HPMA in the presence of beta-CD. Hence, three HPMA-beta-CDs and a pure HPMA were synthesized by Michael addition polymerization. As a model drug, CLB (an anti-cancer drug) was loaded into them via a solution method for in vitro release studies. The DSC results indicate that the CLB/polymer interactions are at the molecular level. Loading CLB into these polymers results in an evident increase in their glass transition temperatures, and DeltaT(g) depends on the beta-CD content. The controlled-release experiments show that the presence of beta-CD can appropriately slow the release of CLB from HPMA-beta-CDs and adjust the ratio of CLB released in total drug loading.

This paper studied the complex self-assembly of hyperbranched polyamidoamine (h-PAMAM) and linear polyaryalic acid (l-PAA) by the facile mixing of their aqueous solutions. The complex self-assembly behavior and mechanism were investigated by the optical microscopy, UV-vis spectrometer, TEM, and zeta potential measurements. Interestingly, various self-assembled aggregates from micelles to microscaled vesicles were obtained by adjusting the solution pH. Moreover, the hollow structure of the vesicles was successfully stabilized by using glutaric dialdehyde (GDA) to cross-link the PAMAM layer. As expected, the resulting hollow spheres were able to capture different noble metal ions from their aqueous solution and reduce them into nanoparticles in situ, hence forming the hybrid hollow spheres. Such hybrid hollow spheres might have potential application in catalytic fields.

A series of colloid silver or gold nanoparticles (AgNPs or AuNPs) were successfully prepared by in situ reduction and stabilization of hyperbranched poly(amidoamine) with terminal dimethylamine groups (HPAMAM-N(CH(3))(2)) in water, and they all exhibited highly antimicrobial activity. The particle size could be controlled easily by adjusting the molar ratio of N/Ag (or N/Au) in feed. When the molar ratio was 2, some aggregates of the nanoparticles separated from the colloidal solution, which showed some limited antimicrobial activity with the bacterial inhibition ratio of below 15%. As the molar ratio increased from 10 to 30, the average particle diameters decreased (from ca. 7.1 to 1.0 nm for AgNPs and from ca. 7.7 to 3.9 nm for AuNPs, respectively) and they all showed high dispersion stability and excellent antimicrobial efficiency. All the bacterial inhibition ratios reached up to ca. 98% at the low silver content of ca. 2.0 microg/mL or at the low gold content of ca. 2.8 microg/mL. The AgNPs or AuNPs with smaller particle size can provide much more effective contact surface with the bacteria, thus enhancing their antimicrobial efficiency. Besides, the cationic HPAMAM-N(CH(3))(2) can also do some contribution to the antimicrobial activity through the strong ionic interaction with the bacteria.

The chemical nature, the mode of action, and the in vitro and in vivo anti-HSV activities of the polysaccharide from Prunella vulgaris were characterized. The polysaccharide was isolated by ethanol precipitation, dialysis, CTAB precipitation, and gel exclusion chromatography. The isolated compound (PPS-2b) was a lignin-carbohydrate complex with a molecular weight of 8500. The carbohydrate moiety was composed of glucose, galactose, mannose, galacturonic acid, rhamnose, xylose, and arabinose with glucose as the major sugar. In plaque reduction assay, PPS-2b showed activities against HSV-1 and HSV-2. The anti-HSV activity could be abolished by periodate oxidation. Mechanism studies showed that PPS-2b inactivated HSV-1 directly, blocked HSV-1 binding to Vero cells, and inhibited HSV-1 penetration into Vero cells. A similar inhibition was observed with a gC-deficient strain of HSV-1. The in vivo activities of a Prunella cream formulated with a semi-purified fraction was assessed in a HSV-1 skin lesion model in guinea pigs and a HSV-2 genital infection model in BALB/c mice. Guinea pigs that received the Prunella cream treatment showed a significant reduction (P<0.01) in skin lesions. Mice that received the Prunella cream treatment showed a significant reduction (P<0.01) in mortality. In conclusion, the anti-HSV compound from P. vulgaris is a lignin-polysaccharide complex with potent activity against HSV-1 and HSV-2. Its mode of action appears to be inhibiting viral binding and penetration into host cells.

A novel hyperbranched polymer gelator has been synthesized, which can self-assemble into the thermoreversible physical gel in DMF, DMAC, pyridine, DMSO or NMP with the driving force of hydrogen bonds among amide and amine groups of the highly branched macromolecules.