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1. This study assessed the addition of dried stinging nettle (SN) leaves at 0%, 2.5% and 5.0% in feed on egg production, egg quality, chemical composition and antioxidant content in eggs from laying hens.2. Seventy-two Hy-Line Brown laying hens, housed in 36 enriched layer cages, were used in the study from 43 to 47 weeks of age. Feeding dry SN leaves did not affect ( p  > 0.05) egg production variables.3. Dietary SN inclusion linearly increased ( p  < 0.001) carotenoid content more than six-fold, in addition to yellowness and redness of the yolks at the maximum inclusion. Providing eggs from hens fed carotenoid enriched diets, e.g . SN, may be used to increase carotenoids in human diets.

Lanthanides, a group of elements with unique chemical properties, have garnered significant attention for their varied biological effects, ranging from cytotoxic to protective, depending on concentration, cell type, and exposure conditions. This review provides a detailed examination of the biological interactions of lanthanides with mammalian systems, including humans, by exploring their impact on different cell lines and organisms. Through a systematic assessment of current research, this work highlights the dual nature of lanthanides, identifying them as both potential therapeutic agents and environmental toxins. Furthermore, it underscores the importance of understanding their mechanisms to mitigate health risks, particularly for those exposed occupationally or via environmental sources. The review concludes with an overview of knowledge gaps and future research directions necessary for unlocking the therapeutic potential of lanthanides while ensuring safety and sustainability in their applications., Competing Interests: Declaration of Competing Interest ☒ The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024. Published by Elsevier Inc.)

Microplastics pose detrimental effects on the environment, aquatic products, and human health. This comprehensive analysis highlights the repercussions of microplastic contamination within aquaculture. Microplastics in aquaculture are primarily from land-based plastic waste, tourism-related disposal, shipping activities, fisheries/aquaculture, and atmospheric deposition. In aquaculture environments, microplastics have the potential to discharge harmful additives, attract pollutants, degrade the aquaculture setting, and induce toxicological effects. These particles pose ecological risks and can impact human health. Assessing the destiny of microplastics in aquaculture ecosystems is crucial to determining the role of aquaculture in contributing to microplastic contamination within the watershed. It particularly emphasizes the ecological consequences for aquaculture species and the subsequent health threats for humans. The review strongly supports strict regulations to control and limit microplastic presence within aquaculture ecosystems. Clear regulations are essential for reducing microplastics in aquaculture, thereby ensuring food safety. A novel nature-based solution is proposed using methods like microplastic biofilters, biodegradation, and wetlands. These innovations can be conducted in aquatic ecosystems to serve as microplastic biofilters, effectively eliminating waterborne microplastics. In the future, however, it is crucial to develop additional emergency treatment measures to avoid the potential negative impacts of microplastics on both aquaculture and human health., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier Ltd. All rights reserved.)

Bisphenol A (BPA), a synthetic chemical widely used in the production of polycarbonate plastic and epoxy resins, has been associated with a variety of adverse effects in humans including metabolic, immunological, reproductive, and neurodevelopmental effects, raising concern about its health impact. In the EU, it has been classified as toxic to reproduction and as an endocrine disruptor and was thus included in the candidate list of substances of very high concern (SVHC). On this basis, its use has been banned or restricted in some products. As a consequence, industries turned to bisphenol alternatives, such as bisphenol S (BPS) and bisphenol F (BPF), which are now found in various consumer products, as well as in human matrices at a global scale. However, due to their toxicity, these two bisphenols are in the process of being regulated. Other BPA alternatives, whose potential toxicity remains largely unknown due to a knowledge gap, have also started to be used in manufacturing processes. The gradual restriction of the use of BPA underscores the importance of understanding the potential risks associated with its alternatives to avoid regrettable substitutions. This review aims to summarize the current knowledge on the potential hazards related to BPA alternatives prioritized by European Regulatory Agencies based on their regulatory relevance and selected to be studied under the European Partnership for the Assessment of Risks from Chemicals (PARC): BPE, BPAP, BPP, BPZ, BPS-MAE, and TCBPA. The focus is on data related to toxicokinetic, endocrine disruption, immunotoxicity, developmental neurotoxicity, and genotoxicity/carcinogenicity, which were considered the most relevant endpoints to assess the hazard related to those substances. The goal here is to identify the data gaps in BPA alternatives toxicology and hence formulate the future directions that will be taken in the frame of the PARC project, which seeks also to enhance chemical risk assessment methodologies using new approach methodologies (NAMs).

The increasing environmental challenges caused by pharmaceutical waste, especially antibiotics and contaminants, necessitate sustainable solutions. Cellulose-based membranes are considered advanced tools and show great potential as effective materials for the removal of drugs and organic contaminants. This review introduces an environmentally friendly composite membrane for the elimination of antibiotics and dye contaminants from water and food, without the use of toxic additives. The potential of cellulose-based membranes in reducing the impact on water quality and promoting environmental sustainability is emphasized. Additionally, the benefits of using biobased cellulose membranes in membrane biological reactors for the removal of antibiotics from pharmaceutical waste and milk are explored, presenting an innovative approach to achieving a circular economy. This review provides recent and comprehensive insights into membrane bioreactor technology, making it a valuable resource for researchers seeking efficient methods to break down antibiotics in industrial wastewater, particularly in the pharmaceutical and dairy industries.

Astaxanthin (AXT), a natural carotenoid, has strong antioxidant and anti-ageing effects and can reduce ultraviolet light-induced damage to cells and DNA, stimulate the immune system, and improve cardiovascular disease prognosis. Despite its wide applications in the: nutraceutical, cosmetic, aquaculture, and pharmaceutical industries, AXT industrial production and application are hindered by natural source scarcity, low production efficiency, and high requirements. This review compares the qualitative differences of AXT derived from different natural sources, evaluates the upstream procedures for AXT expression in different chassis organisms, and investigates synthetic biology- and cell factory-based strategies for the industrial production of natural AXT. Synthetic biology is a promising novel strategy for reprogramming plants or microorganisms to produce AXT. Additionally, genetic engineering using cell factories extends beyond terrestrial applications, as it may contribute to the long-term sustainability of human health during space exploration and migration endeavors. This review provides a theoretical basis for the efficient and accurate genetic engineering of AXT from the microalga Haematococcuspluvialis , providing a valuable reference for future research on the biomanufacturing of AXT and other biological metabolites.

Milk has been globally recognised as a comprehensive and vital food source for centuries. However, the presence of heavy metals and metalloids (metal(loid)s) in milk is a global problem. As metal(loid)s are present in the soil due to natural geogenic and various anthropogenic activities, these metal(loid)s are bio-transferred into animal feed, which further results in the presence of metal(loid)s in milk due to bio transfer/accumulation. This systematic review collated information from published literature between 2000 and 2021. It focused on the global issue of metal(loid)s in milk, posing potential health risks. These contaminants enter the food chain through the bio-transfer/accumulation process from soil to animal feed to milk. The key metal(loid)s examined are arsenic (As), mercury (Hg), lead (Pb), and cadmium (Cd). A meta-analysis of 66 selected papers revealed the widespread presence of these contaminants in milk samples globally, with Pb being the most studied (43 %). This research estimated metal(loid)s levels or concentrations as 12.71 (95 % Confidence Interval (CI) = 0.16-25.26), 16.09 (95 % CI = 4.31-27.70), 197.04 (95 % CI = 75.28-318.18), 31.67 (95 % CI = 20.14-43.20) μg/kg (ppb) for As, Hg, Pb, and Cd, respectively using Stata™. The metal(loid) concentrations in milk were within the threshold limits other than Pb and Cd. Some studies in America, Africa, and Asia reported elevated Pb and Cd concentrations, raising health concerns. The simulated Risk Quotients (RQ) and Integrated Risk Quotient (IRQ) values generally remain above one, indicating potential human health risks. Notably, the IRQ value increases with more metal(loid)s consideration. Subgroup analysis indicates low-fat milk contains higher metal(loid)s concentrations. While metal(loid)s concentrations in milk largely comply with safety limits, some regions exhibit concerning concentrations. Therefore, continued surveillance to address potential health risks associated with metal(loid)s in milk is necessary to ensure dairy products' safety., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 The Authors. Published by Elsevier B.V. All rights reserved.)

Antimicrobial resistance is an increasing societal burden worldwide, with ESKAPEE ( Enterococcus faecium , Staphylococcus aureus , Klebsiella pneumoniae , Acinetobacter baumannii , Pseudomonas aeruginosa , Enterobacter species and Escherichia coli ) pathogens overwhelming the healthcare sectors and more recently becoming predominantly a concern for their persistence in food and food industries, including agricultural settings and animal husbandry environments. The aim of this review is to explore the mechanisms by which the ESKAPEE group gained its multidrug resistance profiles, to analyse their occurrence in different foods and other related reservoirs, including water, and to address the current challenges due to their spread within the food production chain. Moreover, the repertoire of surveillance programmes available focused on monitoring their occurrence, common reservoirs and the spread of antimicrobial resistance are described in this review paper. Evidence from the literature suggests that restricting our scope in relation to multidrug resistance in ESKAPEE pathogens to healthcare and healthcare-associated facilities might actually impede unveiling the actual issues these pathogens can exhibit, for example, in food and food-related reservoirs. Furthermore, this review addresses the need for increasing public campaigns aimed at addressing this challenge, which must be considered in our fight against antimicrobial resistance shown by the ESKAPEE group in food and food-related sectors.

Plants are continuously exposed to environmental challenges, including pollutants, pesticides, and heavy metals, collectively termed xenobiotics. These substances induce oxidative stress by generating reactive oxygen species (ROS), which can damage cellular components such as lipids, proteins, and nucleic acids. To counteract this, plants have evolved complex metabolic pathways to detoxify and process these harmful compounds. Oxidative stress in plants primarily arises from the overproduction of hydrogen peroxide (H 2 O 2 ), superoxide anions (O 2 •- ), singlet oxygen ( 1 O 2 OH), by-products of metabolic activities such as photosynthesis and respiration. The presence of xenobiotics leads to a notable increase in ROS, which can result in cellular damage and metabolic disruption. To combat this, plants have developed a strong antioxidant defense mechanism that includes enzymatic antioxidants that work together to eliminate ROS, thereby reducing their harmful effects. In addition to enzymatic defenses, plants also synthesize various non-enzymatic antioxidants, including flavonoids, phenolic acids, and vitamins. These compounds effectively neutralize ROS and help regenerate other antioxidants, offering extensive protection against oxidative stress. The metabolism of xenobiotic substances in plants occurs in three stages: the first involves modification, which refers to the chemical alteration of xenobiotics to make them less harmful. The second involves conjugation, where the modified xenobiotics are combined with other substances to increase their solubility, facilitating their elimination from the plant. The third stage involves compartmentalization, which is the storage or isolation of conjugated xenobiotics in specific parts of the plant, helping to prevent damage to vital cellular functions. Secondary metabolites found in plants, such as alkaloids, terpenoids, and flavonoids, play a vital role in detoxification and the defense against oxidative stress. Gaining a deeper understanding of the oxidative mechanisms and the pathways of xenobiotic metabolism in plants is essential, as this knowledge can lead to the formulation of plant-derived strategies aimed at alleviating the effects of environmental pollution and enhancing human health by improving detoxification and antioxidant capabilities, as discussed in this review.• OH), by-products of metabolic activities such as photosynthesis and respiration. The presence of xenobiotics leads to a notable increase in ROS, which can result in cellular damage and metabolic disruption. To combat this, plants have developed a strong antioxidant defense mechanism that includes enzymatic antioxidants that work together to eliminate ROS, thereby reducing their harmful effects. In addition to enzymatic defenses, plants also synthesize various non-enzymatic antioxidants, including flavonoids, phenolic acids, and vitamins. These compounds effectively neutralize ROS and help regenerate other antioxidants, offering extensive protection against oxidative stress. The metabolism of xenobiotic substances in plants occurs in three stages: the first involves modification, which refers to the chemical alteration of xenobiotics to make them less harmful. The second involves conjugation, where the modified xenobiotics are combined with other substances to increase their solubility, facilitating their elimination from the plant. The third stage involves compartmentalization, which is the storage or isolation of conjugated xenobiotics in specific parts of the plant, helping to prevent damage to vital cellular functions. Secondary metabolites found in plants, such as alkaloids, terpenoids, and flavonoids, play a vital role in detoxification and the defense against oxidative stress. Gaining a deeper understanding of the oxidative mechanisms and the pathways of xenobiotic metabolism in plants is essential, as this knowledge can lead to the formulation of plant-derived strategies aimed at alleviating the effects of environmental pollution and enhancing human health by improving detoxification and antioxidant capabilities, as discussed in this review.

This review summarizes methods and relevant outcomes of aerobiological studies carried out in the alpine biome worldwide impacting the knowledge on the occurrence of airborne pollen and their origin, for biodiversity studies, models of transport, forecasts, and climate change scenarios, for the reconstruction of past vegetation, and the potential impacts on human health. Deposition sampling is the method of choice, while volumetric sampling is mostly performed in densely populated mountain ranges. Conventional microscopic identification of pollen of alpine environments is rarely complemented or replaced by molecular methods. The pollen bioaerosol mirrors the surrounding vegetation but includes components from medium and distant source locations. However, there is no uniform understanding on the definition of source-scales - crucial for the interpretation of the bioaerosol constituents - to which we propose an answer. Alpine habitats, with their cold-adapted plant communities, may react to increasing temperatures with shifts in their range. The potential of using pollen as a proxy to monitor such changes in alpine biomes has been exploited in paleoecology but rarely in aerobiology. Health impacts are linked to the low allergen load in the bioaerosol and the overall effect of the alpine climate in a highly natural environment. Generally, the soothing effect is reported for respiratory allergy patients, which may be jeopardized by seasonality and allergens transported from outside. The complex topography of mountain ranges does not allow for general assumptions on the quality and quantity of bioaerosol in alpine environments. We emphasize the importance of monitoring the bioaerosol in alpine environments to evaluate the effects of global change, and to optimize the management of respiratory health issues., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 The Authors. Published by Elsevier B.V. All rights reserved.)

Wastewater-based epidemiology (WBE) is a powerful tool to gather epidemiological insights at the community level, providing objective data on population exposure to harmful substances. A considerable portion of the human exposure to these potentially harmful chemicals occurs unintentionally, unlike substances such as pharmaceuticals, illicit drugs, or alcohol. In this context, this comprehensive review analyzes WBE studies focused on classes of organic chemicals to which humans are unintentionally exposed, namely organophosphorus flame retardants, per- and polyfluoroalkyl substances (PFAS), benzotriazoles and benzothiazoles, phthalates and terephthalates, benzophenones, pesticides, bisphenols, and parabens. The review highlights some advantages of WBE for public health surveillance, e.g., non-invasive analysis, predictive capability, nearly real-time data, population-wide insights, no ethical approval, and unbiased sampling. It also discusses challenges and future research directions in WBE regarding exposure to harmful chemicals from various sources. The review emphasizes the critical role of wastewater sampling, sample preparation, quality control, and instrumental analysis in achieving accurate and reliable results. Furthermore, it examines the selection of human biomarkers for WBE studies and explores strategies to link WBE with human biomonitoring (HBM), which together enhance both the precision and effectiveness of exposure assessments., (© 2024. The Author(s).)

This review offers a novel perspective on the environmental fate and ecotoxicological effects of tire wear particles (TWPs), ubiquitous environmental contaminants ranging in size from micrometers to millimeters (averaging 10-100 micrometers). These particles pose a growing threat due to their complex chemical composition and potential toxicity. Human exposure primarily occurs through inhalation, ingesting contaminated food and water, and dermal contact. Our review delves into the dynamic interplay between TWP composition, transformation products (TPs), and ecological impacts, highlighting the importance of considering both individual chemical effects and potential synergistic interactions. Notably, our investigation reveals that degradation products of certain chemicals, such as diphenylguanidine (DPG) and diphenylamine (DPA), can be more toxic than the parent compounds, underscoring the need to fully understand these contaminants' environmental profile. Furthermore, we explore the potential human health implications of TWPs, emphasizing the need for further research on potential respiratory, cardiovascular, and endocrine disturbances. Addressing the challenges in characterizing TWPs, assessing their environmental fate, and understanding their potential health risks requires a multidisciplinary approach. Future research should prioritize standardized TWP characterization and leachate analysis methods, conduct field studies to enhance ecological realism, and utilize advanced analytical techniques to decipher complex mixture interactions and identify key toxicants. By addressing these challenges, we can better mitigate the environmental and health risks associated with TWPs and ensure a more sustainable future., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024. Published by Elsevier B.V.)

Microplastics (MPs) are pervasive across ecosystems, likely posing significant environmental and health risks based on more and more evidence. In this study, we searched through the Web of Science Core Collection and obtained 1039 papers for visualization and analysis. In order to discuss the chemical composition, migration, transformation and potential risk of MPs, 135 sets of relevant data in soil, water, and atmosphere were collected in China as a typical region, which is a hotspot region for investigation of MPs. The results showed that the primary polymer categories of MPs in the environment to be polypropylene, polyethylene, and polystyrene. The soil contains a significant quantity of MPs, averaging at 12,107.42 items·kg dw -1 , while water contains averaging at 97,271.18 items m -3 . The total pollution load indexes for all three environments are at risk level I. Based on current risk assessment methods, the potential ecological risk of MPs is low. However, based on the polymer components, migration and transformation patterns, and especially the complexes with other pollutants, it indicates an increasing indirect risk. Interactions with some other pollutants are likely amplify the ecological and health risks associated with MPs. Aggregative results showed that the present risk assessment models could not assess the risks of MPs well. Thus, we suggested develop a risk assessment methodology for MPs based on relevant research progress. Some factors such as the size and form of MPs, sources and distribution, bioaccumulation, social acceptance and economic costs could be considered adding in the present risk assessment models. Finally, promotion of development and application of green chemically synthesized bioplastics such as using synthetic biology to help degrade plastics would be an alternative and sustainable option to relieve the adverse environmental and health concerns of MPs., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 The Authors. Published by Elsevier Inc. All rights reserved.)

Microplastics (MPs) are synthetic solid particles or polymer matrices that range in size from 1 μm to 5 mm. MPs are widely present in the global biosphere, leading to increasing concerns about their impact on human health. In this study, micro-Raman spectroscopy was used to evaluate the presence and characteristic of MPs in adenomyosis, ovarian ectopic cysts, and uterine tube tissue samples from 60 females. MPs were detected in all human samples at an average level of 1.5 ± 1.2 particles per g of tissue (Average 1.40 ± 1.11 particles per g of tissue after blank correction.). Among these MPs, a total of 11 polymer types were identified. MPs are mainly composed of polyethylene (PE, 31 %), polypropylene (PP, 22 %) and PE-co-PP (11 %). These MPs had an average length of 15.15 ± 6.45 μm and an average width of 12.56 ± 6.65 μm, with the majority (70 %) measuring less than 20 μm in size. Most MPs were fibers (38.9 %) and fragments (24.4 %). A significant correlation (p <0.05) was found between the sizes of MPs detected across the three disease samples, with PE and PP being the most frequently identified types. This study demonstrates the presence of MPs in diseased tissues from patients with adenomyosis, ovarian ectopic cysts, and uterine tubes, providing evidence for the presence of MPs in reproductive system tissues., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 The Authors. Published by Elsevier Inc. All rights reserved.)

Microplastic contamination is a burgeoning environmental issue that poses serious threats to animal and human health. Microplastics enter the human body through nasal, dermal, and oral routes to contaminate multiple organs. Studies have advocated the existence of microplastics in human breast milk, sputum, faeces, and blood. Microplastics can find their ways to the sub-cellular moiety via active and passive approaches. At cellular level, microplastics follow clathrin and caveolae-dependent pathways to invade the sub-cellular environment. These environmental contaminants modulate the epigenetic control of gene expression, status of inflammatory mediators, redox homeostasis, cell-cycle proteins, and mimic the endocrine mediators like estrogen and androgen to fuel carcinogenesis. Furthermore, epidemiological studies have suggested potential links between the exposure to microplastics and the onset of various chronic diseases. Microplastics trigger uncontrolled cell proliferation and ensue tissue growth leading to various cancers affecting the lungs, blood, breasts, prostate, and ovaries. Additionally, such contamination can potentially affect sub-cellular signaling and injure multiple organs. In essence, numerous reports have claimed microplastic-induced toxicity and tumorigenesis in human and model animals. Nonetheless, the underlying molecular mechanism is still elusive and warrants further investigations. This review provides a comprehensive analysis of microplastics, covering their sources, chemistry, human exposure routes, toxicity, and carcinogenic potential at the molecular level., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier Inc. All rights reserved.)

Organic ultraviolet filters (OUVFs) are extensively incorporated into both cosmetic items and industrial products and have been commonly found in water ecosystems. This study aims to examine the environmental levels, sources, ecological and human health risks of 14 commonly used OUVFs both in coastal water and beach deposit samples collected from the nearshore regions of Hainan Island and the South China Sea. This is first study highlighting the contamination of OUVFs in Hainan Island and utilizing economic and tourism data to confirm the potential source of OUVF pollution in costal aquatic and coastal ecosystem. Along the coastal tourist regions of Hainan Island, the median concentrations in coastal waters and beach deposits of these OUVFs fall within the range from 1.2 to 53.2 ng/L and 0.2-17.0 ng/g dw, respectively. In coastal water and beach deposit, the concentration of BP-3 was the highest, with median concentrations of 53.2 ng/L and 17.0 ng/g dw, respectively. Regarding human health risks, the daily intake of all 14 OUVFs through swimming was found to be 40-48 ng/kg/day. Ecological risk assessment indicates that BP-3 presents a medium risk for marine microalgae with a concurrent low risk for corals. The correlation analysis underscores a substantial interrelation of OUVFs in both coastal waters and beach deposits with various economic indicators, including annual rainfall, overnight tourists, total hotel rooms (unit), room occupancy rate, and sewage treatment capacity., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier Ltd. All rights reserved.)

In recent years, an increase in the number of chronic kidney disease (CKD) cases has led to a global health burden majorly affecting underdeveloped and developing nations. A key biomarker for assessing the kidneys' normal functioning is creatinine, which is filtered out from the blood by the kidney. Thus, timely and specific detection of creatinine becomes necessary for diagnosis and subsequent treatment of CKD. Hence, in this study, we have tried to develop a field-deployable, software-integrated immunosensor for the detection of creatinine in a serum sample. The immunosensor was developed by incorporating gold nanoparticles, boron doped MXene, polyaniline, and anticreatinine antibody using an appropriate bioconjugation reaction. The developed sensor was able to detect creatinine in a linear dynamic range of 10 nM to 0.1 M with a limit of detection of 1.72 (±0.07) nM. The sensor was integrated with an indigenously developed software named "CretCheck" which simplifies the process of data analysis. The software integrated personalized biosensing device was used to find the creatinine concentrations directly from the obtained analytical signals. The developed immunosensor with the integrated software can also be implemented directly in primary health care facilities for creatinine detection in the future.

In recent years, overexplorations of ore and the growth of industries are the prime factors in the release of heavy metals in environments. As a result, the food crops and water bodies are contaminated with metals which may have several adverse effects on the health of humans and other living species. These metals and metalloids, such as Zn, Cu, Mn, Ni, Cr, Pb, Cd, and As, upset the biochemical pathways of metabolite synthesis in living organisms and contribute to the etiology of different diseases. Microorganisms include bacteria, archaea, viruses, and many unicellular eukaryotes, which can span three domains of life-Archaea, Bacteria, and Eukarya-and some microorganisms, such as cyanobacteria, have shown high efficiency in the biosorption rate of heavy metals. Cyanobacteria are suitable for bioremediation as they can grow in adverse environments, have a less negative impact on the surrounding environment, and are relatively cheaper to manage. The structure of cyanobacteria has shown no extensive internal-bound membranes, so it can directly employ the physiological mechanisms to uptake heavy metals from contamination sites. Such biochemical makeups are suitable for managing and bioremediating heavy metal concentrations in polluted environments. This review aims to explore the potential of cyanobacteria in the bioremediation of heavy metals and metalloids in water bodies. Additionally, we have identified the prospects for enhancing bioremediation effectiveness., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2024 Lourembam, Haobam, Singh, Verma and Rajan.)

This review summarizes studies on the relationships between climate change and Valley Fever (VF), also termed Coccidioidomycosis, a potentially fatal upper-respiratory fungal infection caused by the pathogenic fungi, C. immitis or C. posadasii. The intensified onset of climate change has caused frequencies and possibly intensities of natural hazard events like dust storms and drought to increase, which has been correlated with greater prevalence of VF. These events, followed by changes in patterns of precipitation, not only pick up dust and spread it throughout the air, but also boost the growth and spread of Coccidioides. In California alone, cases of VF have increased fivefold from 2001 to 2021, and are expected to continue to increase. From 1999 to 2019, there was an average of 200 deaths per year caused by VF in the United States. The number of deaths caused by VF fluctuates year to year, but because more infections are predicted to occur due to a changing climate, deaths are expected to rise; thus, the rising prevalence of the disease is becoming a larger focus of the scientific community and poses an increased threat to public health. By reviewing recent and past studies on Coccidioidomycosis and its relationships with climate factors, we categorize future impacts of this disease on the United States, and highlight areas that need more study. Factors affecting the incidence of VF, such as modes of dispersal and the optimum environment for Coccidioides growth, that could potentially increase its prevalence as weather patterns change are discussed and how the endemic regions could be affected are assessed. In general, regions of the United States, including California and Arizona, where VF is endemic, are expanding and incidences of VF are increasing in those areas. The surrounding southern states, including Nevada, New Mexico, Utah, and Texas, are experiencing similar changes. In addition, the entire endemic region of the United States is predicted to spread northward as drought is prolonged and temperatures steadily increase. The findings from the keyword search from eight databases indicate that more studies on VF and its relation to dust and climate are needed especially for endemic states like Nevada that are currently not adequately studied. Overall, results of this survey summarize mechanisms and climate factors that might drive spread of VF and describes trends of incidence of VF in endemic states and predicted likely trends that might occur under a changing climate. Through reviewing recent and past studies of Coccidioidomycosis and its relationships with climate factors, future impacts of this disease have been categorized and speculated on effects it might have on the United States. Better understanding of how climate factors affect VF as well as identifying regions that require more research could inform both environmental managers and medical professionals with the resources needed to make more accurate predictions, design better mitigation strategies, send timely warnings, and protect public health. Shortened version This review explores how climate change affects Valley Fever (VF), a dangerous fungal infection caused by C. immitis or C. posadasii. Climate change has increased natural hazard events such as dust storms and droughts, which have caused the spread of VF. Cases of the disease have increased fivefold between 2001 and 2021 in California alone, and it poses an increasing threat to public health. The review summarizes mechanisms that drive the spread of VF and highlights trends in endemic states under a changing climate. It recommends more studies on VF and its relation to dust and climate, especially for states like Nevada. Identifying regions that require more research can help make more accurate predictions, design better mitigation strategies, send timely warnings, and protect public health., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 The Authors. Published by Elsevier Ltd.. All rights reserved.)

Honey is an attractive functional food that often becomes a subject of clinical studies on the treatment of diverse diseases. However, the clinical efficacy of honey is rather controversial due, at least in part, to its variable composition and botanical origin as well as thermal processing or improper storage conditions. This review addresses the importance of honey quality standards and in vitro testing of the biological properties of honey prior to performing clinical studies, which can have a great impact on clinical outcomes. It focused on recently performed meta-analyses and systematic reviews where honey was used in the management of various disorders including respiratory tract infections, and metabolic and cardiometabolic diseases, with the goal of characterising the honeys used in clinical studies. In addition, it provides recommendations for the use and storage of honey for clinical testing. The vast majority of clinical studies included in meta-analyses do not provide any information about honey quality parameters. In fact, indicators of thermal damage or prolonged storage of honey were analysed only in one clinical study. This observation highlights on the alarming status of honey quality in clinical studies. Furthermore, in vitro biological properties of the analysed honeys were assessed in two clinical studies. Therefore, this review strongly advocates the clinical use of only fully characterised honey samples of known botanical origin with proven in vitro biological functionality and no or minimal thermal processing., Competing Interests: The author declares that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2024 Majtan.)

Microplastic (MP) pollution in urban environments is a pervasive and complex problem with significant environmental and human health implications. Although studies have been conducted on MP pollution in urban environments, there are still research gaps in understanding the exact sources, regulation, and impact of urban MP on the environment and public health. Therefore, the goal of this study is to provide a comprehensive overview of the complex pathways, harmful effects, and regulatory efforts of urban MP pollution. It discusses the research challenges and suggests future directions for addressing MPs related to environmental issues in urban settings. In this study, original research papers published from 2010 to 2024 across ten database categories, including PubMed, Google Scholar, Scopus, and Web of Science, were selected and reviewed to improve our understanding of urban MP pollution. The analysis revealed multifaceted sources of MPs, including surface runoff, wastewater discharge, atmospheric deposition, and biological interactions, which contribute to the contamination of aquatic and terrestrial ecosystems. MPs pose a threat to marine and terrestrial life, freshwater organisms, soil health, plant communities, and human health through ingestion, inhalation, and dermal exposure. Current regulatory measures for MP pollution include improved waste management, upgraded wastewater treatment, stormwater management, product innovation, public awareness campaigns, and community engagement. Despite these regulatory measures, several challenges such as; the absence of standardized MPs testing methods, MPs enter into the environment through a multitude of sources and pathways, countries struggle in balancing trade interests with environmental concerns have hindered effective policy implementation and enforcement. Addressing MP pollution in urban environments is essential for preserving ecosystems, safeguarding public health, and advancing sustainable development. Interdisciplinary collaboration, innovative research, stringent regulations, and public participation are vital for mitigating this critical issue and ensuring a cleaner and healthier future for urban environments and the planet., Competing Interests: Declaration of competing interest All authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier B.V. All rights reserved.)

The majority of human genomic research studies have been conducted in European-ancestry cohorts, reducing the likelihood of detecting potentially novel and globally impactful findings. Here, we present mid-pass whole-genome sequencing data and a genome-wide association study in a cohort of 264 self-reported Malagasy individuals from three locations on the island of Madagascar. We describe genetic variation in this Malagasy cohort, providing insight into the shared and unique patterns of genetic variation across the island. We observe phenotypic variation by location and find high rates of hypertension, particularly in the Southern Highlands sampling site, as well as elevated self-reported malaria prevalence in the West Coast site relative to other sites. After filtering to a subset of 214 minimally related individuals, we find a number of genetic associations with body composition traits, including many variants that are only observed in African populations or populations with admixed African ancestry from the 1000 Genomes Project. This study highlights the importance of including diverse populations in genomic research for the potential to gain novel insights, even with small cohort sizes. This project was conducted in partnership and consultation with local stakeholders in Madagascar and serves as an example of genomic research that prioritizes community engagement and potentially impacts our understanding of human health and disease., Competing Interests: Declaration of interests I.H., M.M., A.-K.E., M.H., S.L.v.B., K.A.W., L.Y.-A., and S.E.C. are employees and option or shareholders of Variant Bio, Inc.; K.A.W. and S.E.C. are co-founders of Variant Bio, Inc., and S.E.C. is a member of its board of directors. L.Y.-A. is a shareholder of GSK., (Copyright © 2024 The Author(s). Published by Elsevier Inc. All rights reserved.)

The Virtual Human Platform for Safety Assessment (VHP4Safety) project aims to build a Virtual Human Platform (VHP) to protect human health and revolutionize the safety assessment of chemicals and pharmaceuticals by transitioning from animal-based to human-based approaches. The goal of this article is to introduce the project and its interdisciplinary approach to co-creation with multiple academic, regulatory, industrial and societal partners covering the entire safety assessment knowledge chain. Three research lines drive the project: 1) building the VHP; 2) feeding the VHP with human data; and 3) implementing the VHP. The project focusses on three case studies that incorporate human relevant scenarios not included in current animal-based safety assessment strategies. The VHP is built on tools and services, including pharmacokinetic and computational models, and integrates several data sources within each case study, including data on human physiology, epidemiology, toxicokinetic and -dynamic parameters, as well as data on chemical characteristics and exposures. In addition, the VHP integrates new data generated within the project using new approach methodologies representing key events within adverse outcome pathways. Implementation of the VHP is investigated using an innovation systems approach, engaging stakeholders and organizing training and education. Central to the VHP4Safety project is our co-creative approach, which facilitated by biannual designathons and hackathons that foster active involvement of all project participants from over 30 partner organizations. By integrating technological innovations with transparency and stakeholder collaboration, the VHP4Safety project will help shape the transition to the next generation safety assessment in which animal testing becomes redundant.

Antibiotic resistance (AR) is a critical global health issue with significant clinical and economic implications. AR occurs when microorganisms develop mechanisms to withstand the effects of antibiotics, reducing treatment efficacy and increasing the risk of mortality and healthcare costs. While the connection between antibiotic use in clinical and agricultural settings and the emergence of AR is well-established, the role of urban soils as reservoirs and spreaders of AR is underexplored. This review examines the complex dynamics of AR in urban soils, highlighting the various sources of antibiotics, including domestic wastewater, industrial effluents, urban agricultural practices, but also microplastics and domestic animal excrements. The selective pressure exerted by these anthropogenic sources promotes the proliferation of antibiotic-resistant bacteria, particularly through horizontal gene transfer, which facilitates the transmission of resistance genes among soil microorganisms in urban environments. About that, the presence of antibiotics in urban soils poses a significant threat to public health by potentially transferring resistance genes to human pathogens through multiple pathways, including direct contact, food consumption, and water ingestion. Furthermore, AR in urban soils disrupts microbial community dynamics, impacting soil fertility, plant growth, and overall environmental quality. Therefore, this review aims to address gaps in understanding AR in urban soils, offering insights into its implications for human health and ecosystem integrity. By identifying these gaps and suggesting evidence-based strategies, this review proposes valid and sustainable solutions to mitigate and counteract the spread of AR in urban environments., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 The Author(s). Published by Elsevier Inc. All rights reserved.)

Probiotics are pivotal in maintaining or restoring the balance of human intestinal microbiota, a crucial factor in mitigating diseases and preserving the host's health. Exploration into Bacteroides spp. reveals substantial promise in their development as next-generation probiotics due to their profound interaction with host immune cells and capability to regulate the microbiome's metabolism by significantly impacting metabolite production. These beneficial bacteria exhibit potential in ameliorating various health issues such as intestinal disorders, cardiovascular diseases, behavioral disorders, and even cancer. Though it's important to note that a high percentage of them are as well opportunistic pathogens, posing risks under certain conditions. Studies highlight their role in modifying immune responses and improving health conditions by regulating lymphocytes, controlling metabolism, and preventing inflammation and cancer. The safety and efficacy of Bacteroides strains are currently under scrutiny by the European Commission for authorization in food processing, marking a significant step towards their commercialization. The recent advancements in bacterial isolation and sequencing methodologies, coupled with the integration of Metagenome-Assembled Genomes (MAGs) binning from metagenomics data, continue to unveil the potential of Bacteroides spp., aiding in the broader understanding and application of these novel probiotics in health and disease management., (© 2024. The Author(s).)

Artificial light at night (ALAN) is a growing environmental issue associated with adverse health consequences, but the association with low birth weight remains unknown. We aim to explore associations between outdoor ALAN and low birth weight across 7,808 census tracts in California. We use data from the California Communities Environmental Health Screening Tool (CalEnviroScreen) at the census tract level. VIIRS Nighttime Day/Night Band Composites Version 1 dataset within the Google Earth Engine platform was used to assess outdoor ALAN. Multivariable linear regression was used to analyze the data. Our findings showed that an interquartile range increase in outdoor ALAN resulted in an increase of 0.42% (95% CI: 0.37, 0.46) in low birth weight rate on average, after adjusting for all potential covariates. The results demonstrate a significant association between outdoor ALAN and low birth weight. These associations remained consistent in sensitivity analyses.

Flowering plants are essentially sessile organisms that disperse their genes through pollination, expanding their areas of occurrence through seed dispersal. In orchids, seed dispersal is commonly mediated by air currents. Conversely, members of several genera have evolved seeds adapted to endozoochory. This is the case for Vanilla, the most economically important genus in the orchid family. The role of indehiscent fruits in the attraction and rewarding of Vanilla seed dispersers was investigated based on field observations, analysis of fragrances, reward substances, and investigation of seed viability through the digestive tract. Indehiscent Vanilla fruits are consumed exclusively by herbivores, i.e. agoutis. Besides providing nutritional rewards, the fruits are rich in polyphenols that are unpalatable to omnivores. The most dominant compound in fruits is attractive only to agoutis. This is the first study showing synzoochory in Orchidaceae and specificity of seed dispersal in orchids. Indehiscent fruits may have evolved early in Neotropical Vanilla in response to selection pressures mediated by large herbivores as the genus emerged ca. 34 Mya in South America, concomitantly with megafauna diversification in the Oligocene. Extinction of the megafauna during the Pleistocene has left agoutis as inheritors of seed dispersal in species with large fleshy fruits. Apart from the effects on omnivores, this study shows that indehiscent fruits of V. chamissonis are consumed exclusively by agoutis, providing the first evidence of target mutualism in orchid seed dispersal., (© 2024 Wiley‐VCH GmbH. Published by John Wiley & Sons Ltd.)

Ultrafine particles (UFP) associated with air quality and health impacts are a major concern in growing urban regions. Concentrations of UFP (particles of size between 10 and 100 nm) and accumulation mode (N acc ) (particles of size >100 and up to 1000 nm), are analyzed over a highly polluted megacity, Delhi, in conjunction with vehicular flow density, during peak (morning, and evening) and non-peak hours. UFP contributes ≥60% to total particle concentration during autumn and monsoon. UFP concentrations are about 50,000 particles per cm 3 in winter which reduces to about 25,000 particles during monsoon. N acc are about 20,000 (winter) and 10,000 (monsoon) particles per cm 3 . UFP concentration and N acc during peak hours are at least twice higher than those obtained in non-peak hours, confirming the dominant influence of emissions from vehicular exhaust in the study region. Seasonal analysis of UFP size distribution reveals that direct emissions dominate the particle concentrations during winter and autumn, whereas new particle formation mechanism contributes the highest in spring and summer. Assessment of inhalable particle number concentration and particle deposition in the human respiratory tract using Multiple Path Particle Dosimetry (MPPD) model, performed for the first time, shows that the order in which these particles deposit in the human respiratory tract is alveoli > bronchiole > bronchus. The deposition ranges between 10 and 18 million nanoparticles during different hours of the day, whereas the estimated inhalable particle concentration (IPN) varies between 0.5 and 1 billion. Results on the IPN during activities classified from light (walking), medium, heavy, very heavy to severe (long-distance running) provide insights into health effects on vulnerable populations. These quantitative results obtained over a megacity on hourly and seasonal variations of nanoparticles along with IPN and deposition rates for different activities are important, and are invaluable inputs for developing mitigation policies aimed to improve air quality and public health, both of which are major concerns in South Asia., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier Ltd. All rights reserved.)

This narrative review describes the settlement of the neonatal microbiome during the perinatal period and its importance on human health in the long term. Delivery methods, maternal diet, antibiotic exposure, feeding practices, and early infant contact significantly shape microbial colonization, influencing the infant's immune system, metabolism, and neurodevelopment. By summarizing two decades of research, this review highlights the microbiome's role in disease predisposition and explores interventions like maternal vaginal seeding and probiotic and prebiotic supplementation that may influence microbiome development., Conclusion: The perinatal period is a pivotal phase for the formation and growth of the neonatal microbiome, profoundly impacting long-term health outcomes., What Is Known: • The perinatal period is a critical phase for the development of the neonatal microbiome, with factors such as mode of delivery, maternal diet, antibiotic exposure, and feeding practices influencing its composition and diversity, which has significant implications for long-term health. • The neonatal microbiome plays a vital role in shaping the immune system, metabolism, and neurodevelopment of infants., What Is New: • Recent studies have highlighted the potential of targeted interventions, such as probiotic and prebiotic supplementation, and innovative practices like maternal vaginal seeding, to optimize microbiome development during the perinatal period. • Emerging evidence suggests that specific bacterial genera and species within the neonatal microbiome are associated with reduced risks of developing chronic conditions, indicating new avenues for promoting long-term health starting from early life., (© 2024. The Author(s).)

Earth's marine ecosystems are changing rapidly, in large part owing to the damaging effects of human activities. Unless humans find better ways of interacting with the seas and oceans, the marine resources upon which we rely will diminish as more ecosystems collapse. The consequences for human health and wellbeing will be severe. The meta-discipline of Oceans and Human Health has catalogued how the oceans and their constituents benefit human lives. Examples include access to seafood, pharmaceuticals and physical and mental health benefits. This interdisciplinary research effort has also revealed how the integrated impact of anthropogenic activities has disrupted ocean processes resulting in extensive losses of marine biodiversity, increasing chemical and microbial pollution, proliferation of harmful algal blooms and increased coastal inundation, all of which threaten human populations. In response, non-governmental organizations and national governments have established various agreements and treaties to prevent further damage, restore what has been lost and grasp new economic opportunities. Nevertheless, ocean-related risks continue to escalate rapidly in the absence of political commitment. New thinking regarding the interconnectedness of all human/ocean interactions is required to remove the barriers and impediments that hamper tackling the wicked problem of fostering health and wellbeing while achieving ocean sustainability., Competing Interests: I declare we have no competing interests., (© 2024 The Authors.)

Many people have associated e-cigarettes with adjectives such as trendy and cool, and regarded vaping as "a safer alternative to cigarette smoking". Compared with the conventional cigarettes, the public is less familiar with the dangers of e-cigarettes. E-cigarettes produce an aerosol by heating the e-liquid (usually comprising nicotine, flavorings, propylene glycol, vegetable glycerin, and other additives). Harmful substances are generated during the vaporization, such as formaldehyde. Cellular damages and DNA hypermethylation are also found to be associated with vaping. We reviewed the clinical neoplastic cases of patients with vaping history (three oral squamous cell carcinomas, one conjunctival squamous intraepithelial neoplasia, and one thoracic NUT-midline carcinoma). These patients tend to be younger than the average cancer patients. Although the exact relations between the cellular damages or DNA hypermethylation and vaping still need further investigations, we should be very careful about the dangers of using e-cigarettes as aids for cigarette smoking cessation., Competing Interests: The authors have no conflicts of interest relevant to this article., (© 2024 Association for Dental Sciences of the Republic of China. Publishing services by Elsevier B.V.)

Life cycle assessment (LCA) along with a survey on epidemiologic and oxidative potential studies was used for analysing the current evidence of the impact of airborne emissions from municipal solid waste incineration (MSWI) on human health. The correspondence among investigated health outcomes and pollutants was discussed based on the Chemical Abstract Service (CAS) and the International Agency for Research on Cancer (IARC). LCA indicated the ability of MSWI in avoiding human health impact, about - 2 × 10 -4 DALY/tonne together with avoided emissions of particulate matter (PM) and resource depletion, about - 2.5 × 10 -3  kg Sb eq /tonne and about - 0.11 kg PM 2.5  eq/tonne, respectively. Positive emissions were detected for greenhouses (about 900 kg CO 2eq /tonne) and ecotoxicity (about 15,000 CTUe/tonne). Epidemiologic studies performed on population exposed to MSWI reported quite contrasting results. In some of these, hazard ratio (HR) ranging from about 0.7 to 2.2 was reported concerning the incidence of stomach, liver, breast and bladder cancer. Larger agreement was detected concerning the incidence of larynx and lung cancer with HR ranging from about 1 to about 2.6. Direct causal nexuses were not definitively identified. Oxidative potential of PM was characterized by a high Pearson correlation > 0.8 to the presence of CrVI, Cu and Zn. These heavy metals were also identified by both CAS and IARC as toxic (i.e. Cu and Zn) and cancerous (i.e. CrVI) substances affecting the organs of both respiratory and digestive apparatus. In general, even if more research is necessary, LCA, oxidative potential and the epidemiologic survey results showed a high level of accordance. This suggests their integrated exploitation for supporting the investigation of both direct and indirect consequences on environment and health related to waste incineration for both retrospective and predictive studies., (© 2024. The Author(s).)

Jellyfish are known for experiencing periodic blooms in population, which occur when their density increases suddenly. The present study assessed the level of heavy metals and proximate composition in the jellyfish Lobonemoides robustus collected from Cox's Bazar coast of Bangladesh. This is the first study conducted in Bangladesh. Most of the studied metals were not possible to detect in L. robustus samples because concentrations were below the detection limit. Ca, Na, Se, and Mg were found to have safe levels in the L. robustus while the amount of Pb was recorded 0.39 ppm. The findings of Target Hazard Quotient, and Carcinogenic Risk indicate that the L. robustus is safe for human consumption (both for children and adults). Hence, it is suitable for consumption and can be exported. This study emphasizes the need for regular marine environment monitoring to ensure that the seafood harvested from these waters is safe for consumption., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier Ltd. All rights reserved.)

Functional foods or drugs based on probiotics have gained unprecedented attention and development due to the increasingly clear relationship between probiotics and human health. Probiotics can regulate intestinal microbiota, dynamically participating in various physiological activities to directly affect human health. Some probiotic-based functional preparations have shown great potential in treating multiple refractory diseases. Currently, the survival and activity of probiotic cells in complex environments in vitro and in vivo have taken priority, and various encapsulation systems based on food-derived materials have been designed and constructed to protect and deliver probiotics. However, traditional encapsulation technology cannot achieve precise protection for a single probiotic, which makes it unable to have a significant effect after release. In this case, single-cell encapsulation systems can be assembled based on biological interfaces to protect and functionalize individual probiotic cells, maximizing their physiological activity. This review discussed the arduous challenges of probiotics in food processing, storage, human digestion, and the commonly used probiotic encapsulation system. Besides, a novel technology of probiotic encapsulation was introduced based on single-cell coating, namely, "armor probiotics". We focused on the classification, structural design, and functional characteristics of armor coatings, and emphasized the essential functional characteristics of armor probiotics in human health regulation, including regulating intestinal health and targeted bioimaging and treatment of diseased tissues. Subsequently, the benefits, limitations, potential challenges, as well as future direction of armor probiotics were put forward. We hope this review may provide new insights and ideas for developing a single-cell probiotics encapsulating system., Competing Interests: Declaration of competing interest All authors declare no potential conflict of interest between them., (Copyright © 2024. Published by Elsevier B.V.)

Plastic has been known as an artificial polymer whereas environmental microplastics become a global concern. Microplastics are reported to cause immunotoxicity in humans through gut deposition and entering the bloodstream. This study is a comprehensive indication of the recent research on microplastic toxicity in the gastrointestinal system. We performed bibliographic analysis using VOS viewer software and analyzed the data received on microplastics and their impact on gut health which has grown exponentially since 2016. Recent findings also support microplastic toxicity in combination with heavy metals. The smaller particle size and other factors enhanced the adsorption ability of environmental contamination such as heavy metals on microplastic which increased their bioaccumulation. Such toxic complexes of heavy metals and microplastics are a concern to natural ecosystems and environmental biologists. Few reports also demonstrated the biofilm formation on microplastic surfaces which might cause greater environmental as well as human health risks. Notably, terms of determining the microplastics in human tissues through several analytical techniques are still limited to some extent. Future research should be focused on the quantification of microplastics in human tissues, the combined effect of microplastics with other contaminants, and their effects on pre-existing diseases. This study boosts understanding of the potential impacts of microplastic and nanoplastic toxicity in the human gastrointestinal system., (© 2023. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.)

Heavy metals contamination of poultry products is a major concern for public health. This study aimed to determine the concentration of mercury (Hg), lead (Pb), and iron (Fe) in the edible parts of broilers, as well as in feed, drinking water, and litter as sources of contamination and to assess their possible human health risk in the province of Jijel (Northeast Algeria). The range of Hg, Pb, and Fe in edible parts were 0.004-0.007, 0.185-0.480, and 28.536-88.306 mg/kg, respectively, and the difference in content was only significant (p < 0.05) for lead. Breast and thigh samples had Pb concentrations above the maximum limit. Spearman coefficient analysis revealed that most correlations were positive between metals detected in feed, water, and litter and those in the edible parts of broilers. But most of them were insignificant (p > 0.05). The estimated daily intake (EDI) values of examined metal exceeded the tolerable daily intake (PTDI). The target hazard quotients (THQ) and hazard index (HI) of all metals were lower than 1, suggesting no significant carcinogenic risks. The calculated incremental lifetime cancer risk (ILCR) of Pb was higher than 10 -4 for men, women, and children, indicating the presence of carcinogenic risk. Considering the wide consumption of broiler meat, regular national monitoring of heavy metals in the broiler production chain is recommended to protect population health., (© 2024. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.)

Wastewater treatment plants (WWTPs) are significant contributors to microplastic (MP) pollution in marine ecosystems when they are inefficient. This study aimed to evaluate the effectiveness of microplastic removal from the effluent of the Anza WWTP (Morocco), which processes industrial and urban wastewater using a lamellar decantation system combined with a submarine emissary for treated water discharge. Additionally, this study investigated the presence of microplastics in the Atlantic seawater where treatment plant effluent is released. Microplastics were collected and extracted from wastewater and seawater samples to assess their abundance, shape, size, polymer type, and removal rates in the treatment plant. The findings revealed an average MP concentration of 1114 ± 90 MPs/L in the influent and 607 ± 101 MPs/L in the effluent, indicating a removal efficiency of 46 %. Seasonal analysis revealed the highest MP concentrations during the summer, with 2181.33 MPs/L in the influent and 1209 MPs/L in the effluent. Seawater samples from the discharge zone of the submarine emissary had an average MP concentration of 1600 MPs/m 3 . Characterization of the MPs revealed that fibers were the most common form of MPs in all the samples. The 500-100 μm size fraction was predominant in the WWTP samples, while MPs smaller than 1 mm were more abundant in the seawater samples. Seven polymer types were identified using attenuated total reflection fourier transform infrared spectroscopy (ATR-FTIR), with PET, PE, PVC, PA, PS, PP, and EVA being the most prevalent. Scanning electron microscopy coupled with energy-dispersive X-ray spectroscopy (SEM/EDX) revealed various degrees of weathering and chemical elements adhering to the MP surfaces. The results of this study provide valuable insights into the effectiveness of conventional treatment systems in removing microplastics and offer a reference for developing management strategies to mitigate MP pollution in Morocco's marine ecosystems., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier B.V. All rights reserved.)

Telomere length is a putative biomarker of aging and is associated with multiple age-related diseases. There are limited data on the landscape of rare genetic variations in telomere length. Here, we systematically characterize the rare variant associations with leukocyte telomere length (LTL) through exome-wide association study (ExWAS) among 390,231 individuals in the UK Biobank. We identified 18 robust rare-variant genes for LTL, most of which estimated effects on LTL were significant (> 0.2 standard deviation per allele). The biological functions of the rare-variant genes were associated with telomere maintenance and capping and several genes were specifically expressed in the testis. Three novel genes (ASXL1, CFAP58, and TET2) associated with LTL were identified. Phenotypic association analyses indicated significant associations of ASXL1 and TET2 with cancers, age-related diseases, blood assays, and cardiovascular traits. Survival analyses suggested that carriers of ASXL1 or TET2 variants were at increased risk for cancers; diseases of the circulatory, respiratory, and genitourinary systems; and all-cause and cause-specific deaths. The CFAP58 carriers were at elevated risk of deaths due to cancers. Collectively, the present whole exome sequencing study provides novel insights into the genetic landscape of LTL, identifying novel genes associated with LTL and their implications on human health and facilitating a better understanding of aging, thus pinpointing the genetic relevance of LTL with clonal hematopoiesis, biomedical traits, and health-related outcomes., (© 2024. The Author(s), under exclusive licence to American Aging Association.)

Legionella pneumophila (L. pneumophila) is a pathogenic bacterium primarily known for causing Legionnaires' Disease which is known for high mortality rates, particularly in the elderly. With caseloads continuing to increase, further research is needed to improve our understanding of optimized sampling schema and safe limits of L. pneumophila, in part to target improved treatment options and realistic population-level risk modeling. Particularly in healthcare and other high-risk locations these become crucial and time sensitive needs. Therefore, we conceptualized this research as a means of incorporating easily measured physiochemical water quality parameters and generalization of the unique ecology of building water systems to build a computational model that can allow for more rapid and accurate decision making. This research uses the specific machine learning (ML) method called statistical learning theory to incorporate concentration of host cells, such as native amoeba, and physiochemical water quality parameters to estimate the probability of observing ranges of Legionella gene copy concentrations. Using data from previously published research on Legionella prevalence in a large building, our ML method trains the model on the relative impacts of physiochemical parameters on likely amoeba host cell occurrences. The model is expanded to estimate host cell concentrations using correlations and regressions operated through LASSO algorithms. After categorization variables from these results are then used to inform a logistic regression to provide an estimate of the probability of Legionella gene copy concentration ranges. In summary, conventional results generated by logistic regression and multiple linear regression quantified the associations among ecological conditions in the water and ability to predict a likely range of Legionella concentration in a management focused way. Further, two ML methods, PCA and LASSO, demonstrated feasibility in accurate real-time monitoring of Legionella through physiochemical indicators as evidenced with good accuracy of predictions based for validation results. Furthermore results demonstrate the vital need to account for the impact of water quality on building on host cells, and via their quantified water microbial ecology, not just Legionella concentrations., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier Ltd. All rights reserved.)

In recent years, advances in nanotechnology have significantly influenced electronics manufacturing, industrial processes, and medical research. Various industries have seen a surge in the use of nanomaterials. However, several researchers have raised the alarm about the toxicological nature of nanomaterials, which appear to be quite different from their crude forms. This altered nature can be attributed to their unique physicochemical profile. They can adversely affect human health and the environment. Nanomaterials that have been released into the environment tend to accumulate over time and can cause a significant impact on the ecosystem and organisms with adverse health effects. Increased use of nanoparticles has led to increased human exposure in their daily lives, making them more vulnerable to nanoparticle toxicity. Because of their small size, nanomaterials can readily cross biological membranes and enter cells, tissues, and organs. Therefore, the effect of nanomaterials on the human environment is of particular concern. The toxicological effects of nanomaterials and their mechanisms of action are being researched worldwide. Technological advances also support monitoring new nanomaterials marketed for industrial and household purposes. It is a challenging area because of the exceptional physicochemical properties of nanomaterials. This updated review focuses on the diverse toxicological perspective of nanomaterials. We have discussed the use of different types of nanoparticles and their physiochemical properties responsible for toxicity, routes of exposure, bio-distribution, and mechanism of toxicity. The review also includes various in vivo and in vitro methods of assessing the toxicity of nanomaterials. Finally, this review will provide a detailed insight into nano material-induced toxicological response, which can be beneficial in designing safe and effective nanoparticles., Competing Interests: The authors declare that they have no competing interests., (© 2024 Qamar et al.)

Background: The Mediterranean diet (MD), globally recognized for its sustainability and health benefits, traditionally emphasizes the consumption of plant-based foods in raw or minimally processed forms. However, shifting lifestyles, even in Mediterranean regions, have led to an increasing consumption of ultra-processed foods (UPF). Epidemiological evidence suggests that UPF consumption may be detrimental to human health, but there is only one clinical trial on this topic which is largely debated in the scientific community. This study aims to investigate the impact of the inclusion of UPF within a Mediterranean-based dietary pattern on cardiometabolic markers, gut microbiota, and other markers of human and planet health., Methods: Fifty clinically healthy individuals showing overweight and presenting a low-to-moderate cardiovascular risk profile will be recruited for a 7-month randomized, open, cross-over dietary trial. Eligible participants will be randomly assigned to a 3-month high-UPF MD (intervention group) or a low-UPF MD (control group), with a 1-month wash-out period. Both intervention diets will have identical food group compositions, with the intervention group consuming 5 servings/day of selected UPF items, and the control group consuming raw/minimally processed items from the same food group. Blood, urine, and fecal samples, alongside food/lifestyle diaries, will be collected from each participant before and after the dietary interventions. The primary endpoint will be the change in plasma LDL-cholesterol levels from baseline. Additional markers include blood pressure, anthropometric parameters, chemical parameters, glucose and lipid-related metabolic markers, incretins, inflammatory and oxidative stress markers, fecal microbiota composition, and short-chain fatty acids. Finally, food waste production will be evaluated through specific validated food diaries. The study has been approved by the Ethical Committee of the University of Milan and the Tuscany Regional Ethics Committee of the Azienda Ospedaliera Universitaria (AOU) - Careggi, Florence., Discussion: Results from the PROMENADE study will improve knowledge about the impact of UPF consumption on human and planet health and will contribute to the scientific debate on this topic., Trial Registration: ClinicalTrials.gov NCT06314932. Registered on March 13, 2024., (© 2024. The Author(s).)

Background: Gallstone disease poses a global threat to human health and is strongly linked to environmental factors. However, there is currently no data on the presence of rare earth elements (REEs) in human gallstones. This paper investigates the concentration and distribution of REEs in gallstones for the first time, aiming to explore the environmental implications on human health., Methods: A total of 25 gallstone samples were collected in Shanghai and the content of REEs was measured by Inductively coupled plasma-Mass Spectrometry (ICP-MS) to explore the distribution of REEs in gallstones., Results: The concentration of REEs in gallstones ranged from 4.89 to 190.8 ng/g (mean 39.21). In most of the gallstone analyses, REEs have been detected and generally attributed to environmental exposure or food contamination. The Y/Ho ratio of gallstones was lower than that of continental rocks, similar to that in the blood, indicating limited fractionation during fluid transport processes in the gallbladder., Conclusions: The upper continental crust (UCC)-normalized REEs pattern in gallstones showed depletion of light REEs, while most showed enrichment of heavy REEs. Positive Gd anomalies were found in most samples, while few samples suggested anthropogenic influence. Whether exogenous inputs or in vivo biofractionation lead to changes in REEs fractionated patterns require further analyses., (© 2024. The Author(s).)

In indoor air the reaction of ozone (O 3 ) with terpenes may lead to the formation of irritating gas-phase products which may induce acute airway effects (i.e. sudden, short-term changes or symptoms related to the respiratory system). We aimed to perform an in vitro study on possible health effects of products from the O 3 -initiated reaction of limonene with printer exhaust, representing real-life mixtures in offices. Human bronchial epithelial cells were exposed for 1 hour (h) to limonene and O 3 , combined with printer exhaust. The resulting concentrations represented 34% and 6% of the generated initial concentrations of limonene (400 µg/m³) and O 3 (417 µg/cm³), respectively, which were in range of high end realistic indoor concentrations. We observed that the reaction of limonene with O 3 generated an increase of ultrafine particles within 1 h, with a significant increase of secondary reaction products 4-oxopentanal and 3-isopropenyl-6-oxo-heptanal at high end indoor air levels. Simultaneous printing activity caused the additional release of micron-sized particles and a further increase in reaction products. Relevant cellular endpoints to evaluate the possible induction of acute airway effects were measured. However, none of the test atmospheres representing office air was observed to induce these effects.

Background: Rheumatoid arthritis (RA) is a debilitating inflammatory disorder characterized by an overactive immune system targeting joints, leading to inflammation and intense pain. While current RA therapies effectively alleviate symptoms, they are often associated with significant side effects. This study aimed to assess the anti-inflammatory and anti-arthritic properties of an Ethanolic Extract of Myxopyrum serratulum A.W. Hill (EEMS) using animal models., Results: The acute toxicity study with EEMS (2000 mg/kg, p.o.) on rats showed no toxicity or mortality up to the highest dose. Inflammation was induced using carrageenan, and rats were treated with varying doses of EEMS (100, 200, and 400 mg/kg, p.o.) and diclofenac to assess anti-inflammatory effects. Anti-arthritic efficacy was evaluated using Complete Freund's adjuvant (CFA)-induced inflammation, comparing EEMS to methotrexate. The results revealed dose-dependent anti-inflammatory effects of EEMS and a reversal of arthritic-induced weight loss in treated groups. Paw volume reduction was significant in both EEMS and methotrexate groups. Biochemical analyses showed elevated markers in the arthritic control group, which were normalized by EEMS and methotrexate. Notably, EEMS (400 mg/kg) significantly reduced cathepsin-D levels compared to the positive control. EEMS administration also lowered hepatic lipid peroxidation and increased endogenous antioxidants (SOD, GSH, and GPX). The 200 and 400 mg/kg doses reduced the iNOS/GADPH ratio, while the 400 mg/kg dose restored cellular and joint structure and significantly decreased IL1 levels., Conclusions: In conclusion, EEMS demonstrated substantial protective effects, mitigating health risks associated with chronic inflammation such as arthritis. These findings underscore the ethnomedical potential of Myxopyrum serratulum as a promising anti-inflammatory and anti-arthritis agent. The study suggests that EEMS could be a viable alternative or complementary therapy for RA, offering therapeutic benefits with potentially fewer side effects than current treatments., (© 2024. The Author(s).)

Carbon dots (CDs) are novel carbon-based luminescent materials with wide-ranging applications in biosensing, bioimaging, drug transportation, optical devices, and beyond. Their advantageous attributes, including biocompatibility, biodegradability, antioxidant activity, photostability, small particle size (<10 nm), and strong light absorption and excitation across a broad range of wavelengths, making them promising candidates in the field of photodynamic therapy (PDT) as photosensitizers (PSs). Further enhancements in functionality are imperative to enhance the effectiveness of CDs in PDT applications, notwithstanding their inherent benefits. Recently, doping agents and solvents have been demonstrated to improve CDs' optical properties, solubility, cytotoxicity, and organelle targeting efficiency. These improvements result from modifications to the CDs' carbon skeleton matrices, functional groups on the surface state, and chemical structures. This review discusses the modification of CDs with heteroatom dopants, dye dopants, and solvents to improve their physicochemical and optical properties for PDT applications. The correlations between the surface chemistry, functional groups, the structure of the CDs, and their optical characteristics toward quantum yield, redshift feature, and reactive oxygen species (ROS) generation, have also been discussed. Finally, the progressive trends for the use of CDs in PDT applications are also addressed in this review., (© 2024 The Author(s). Chemistry - A European Journal published by Wiley-VCH GmbH.)

Fentanyl and its analogues are synthetic opioids of varying potencies that are unfortunately heavily abused. Over the last 15 years, fentanyl and its analogues have contributed to the increasing prominence of hospitalisation and numerous deaths due to drug overdose. In this comprehensive literature review, the mechanism of toxicity of the drug in humans is evaluated. A systematic approach was used whereby the relevant literature has been detailed where the toxicity of fentanyl and/or its analogues to different organs/systems were investigated. Furthermore, the review covers the post-mortem toxicological data and demographic information from past fatal cases where fentanyl was believed to be involved. Such insight into fentanyl toxicity is useful as an aid to better understand the toxic doses of the drug and the suspected mechanism of action and the unexpected complications associated with overdose incidences involving the drug. Finally, the review offers an overview of the traditional and emerging test systems used to investigate the adverse effects of fentanyl on human health.

Excessive fructose consumption, primarily through processed foods and beverages, has become a significant public health concern due to its association with various metabolic disorders. This review examines the impact of fructose on human health, focusing on its role in obesity, insulin resistance, hyperglycemia, type 2 diabetes, uric acid production, and oxidative stress. Fructose metabolism, distinct from glucose, predominantly occurs in the liver, where it bypasses normal insulin regulation, leading to increased fat synthesis through de novo lipogenesis. This process contributes to the development of non-alcoholic fatty liver disease and elevates the risk of cardiovascular disease. Furthermore, fructose-induced adenosine triphosphate depletion activates purine degradation, increasing uric acid levels and exacerbating hyperuricemia. The overproduction of reactive oxygen species during fructose metabolism also drives oxidative stress, promoting inflammation and cellular damage. By synthesizing recent findings, this review underscores the importance of regulating fructose intake, implementing public health policies, and adopting lifestyle changes to mitigate these adverse effects., Competing Interests: Conflicts of interest: In compliance with the ICMJE uniform disclosure form, all authors declare the following: Payment/services info: All authors have declared that no financial support was received from any organization for the submitted work. Financial relationships: All authors have declared that they have no financial relationships at present or within the previous three years with any organizations that might have an interest in the submitted work. Other relationships: All authors have declared that there are no other relationships or activities that could appear to have influenced the submitted work., (Copyright © 2024, Baharuddin et al.)

Bisphenols (BPs), common in plastics, coatings, and resins, are under scrutiny for potential endocrine disruption. Despite banning bisphenol A (BPA), its perceived safer alternatives may still pose health risks, urging thorough studies on their toxicity mechanisms. This study aimed to investigate the cellular toxicity of the top seven most commonly used BPs, bisphenol S (BPS), bisphenol F (BPF), bisphenol AF (BPAF), bisphenol P (BPP), bisphenol AP (BPAP), bisphenol B (BPB), bisphenol E (BPE) in eight different relevant human in vitro cell models: liver (HepaRG), intestinal (Caco-2), breast (T47D), brain (HMC-3), lungs (MRC-5), kidney (HEK293), endothelial (HMEC-1), and skin (HEK-001) cell lines. BPE manifested the highest cytotoxicity in Caco-2 cells, presenting an EC 50 value of roughly 0.2 μM (95% confidence interval). In contrast, HEK293 and HepaRG cells demonstrated significant resilience to BPS (EC 50  > 1000 μM). BPAF, BPP, and BPAP had consistently low EC 50 values across cell lines (6-27.9 μM, 0.6-134.7 μM, and 3.6-178.8 μM), indicating elevated toxicity. After 24 h, all bisphenols adhered to nominal concentrations except BPAF, BPP, and BPS. BPP's concentration notably decreased (30.82 ± 5.53% of nominal value). The results revealed diverse effects of bisphenol analogs on different cell types. These findings emphasized the considerable cytotoxic potential of specific bisphenol analogs across various human cell models, underlining the necessity for a re-evaluation of their safety and regulatory standards., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier Ltd. All rights reserved.)

Airborne fungi and bacteria have been extensively studied by researchers due to their significant effects on human health. We provided an overview of the distribution and sources of airborne pathogenic microbes, and a detailed description of the detrimental effects that these microorganisms cause to human health in both outdoor and indoor environments. By analyzing the large body of literature published in this field, we offered valuable insights into how airborne microbes influence our well-being. The findings highlight the harmful consequences associated with the exposure to airborne fungi and bacteria in a variety of natural and human-mediated environments. Certain demographic groups, including children and the elderly, immunocompromised individuals, and various categories of workers are particularly exposed and vulnerable to the detrimental effect on health of air microbial pollution. A number of studies performed up to date consistently identified Alternaria , Cladosporium , Penicillium , Aspergillus , and Fusarium as the predominant fungal genera in various indoor and outdoor environments. Among bacteria, Bacillus , Streptococcus , Micrococcus , Enterococcus , and Pseudomonas emerged as the dominant genera in air samples collected from numerous environments. All these findings contributed to expanding our knowledge on airborne microbe distribution, emphasizing the crucial need for further research and increased public awareness. Collectively, these efforts may play a vital role in safeguarding human health in the face of risks posed by airborne microbial contaminants., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2024 Al-Shaarani and Pecoraro.)

Bioaerosols originating from animal feeding operations (AFOs) may carry pathogens, allergens, and other hazardous biocomponents, such as endotoxins, posing a potential risk to community health and the environment when dispersed downwind. This review summarizes and synthesizes existing literature data on bioaerosols downwind from three major types of AFOs (swine, poultry, and cattle), covering their composition, concentration, dispersion patterns, measurement methodologies, potential health effects, and mitigation strategies. While many of these bioaerosols are typically detected only near AFOs, evidence indicates that certain bioaerosols, particularly viruses, can travel up to tens of kilometers downwind and remain infectious. Despite the critical importance of these bioaerosols, a refined modeling framework to simulate their transport and fate in downwind air has not yet been developed, nor have source attribution methods been established to track their origins in complex agricultural environments where multiple bioaerosols could co-exist. Therefore, it is imperative to further research downwind bioaerosols from AFOs, including their assessment, modeling, source attribution, and mitigation, to address the public health and environmental challenges associated with animal agriculture., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier B.V. All rights reserved.)