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A multi-point focused laser differential interferometer (FLDI) has been developed to measure density fluctuations at 16 points along a line. A pair of cylindrical lenses on the transmitter side of a conventional single-point FLDI instrument form two closely spaced (≤200 μm), orthogonally polarized, parallel laser lines at the instrument’s focus. On the receiver side of the instrument, the interference of the beams on a 16-element photodiode array results in a single line of measurements. The further addition of a Nomarski prism creates two separate measurement lines, and the addition of a second photodiode array to the instrument enables simultaneous measurements of density fluctuations along the two lines separated by several millimeters. These two lines of measurement can be conveniently oriented at any azimuthal angle relative to the instrument’s optical axis on the measurement plane, coinciding with the instrument’s focus. Two experiments were performed to demonstrate the capabilities of the instrument. In the first experiment, a laser-induced breakdown spark generated a traveling spherical shock wave, and measurements of the resulting density disturbance and wave velocity were obtained. These results were compared to high-speed schlieren images of the shock wave acquired at 400 kHz. In the second experiment, the multi-point FLDI instrument was used to measure density disturbances in the boundary layer of a flat plate in a Mach 6 freestream flow. The measurements were made along two lines, both approximately 6 mm in length, extending from the surface of the plate through the boundary layer. High-speed schlieren images were acquired at 100 kHz during separate wind tunnel runs at matching unit Reynolds numbers to visualize the unsteady boundary layer flow and compare to the FLDI measurements.