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探讨建立兔骨髓间质干细胞 (BMSCs) 的体外分离纯化、扩增和鉴定的方法, 为BMSCs的进一步诱导分化和应用奠定基础。首先抽取兔髂骨骨髓, 采用Percoll密度梯度离心法得到骨髓单个核细胞, 接种后形成单层贴壁细胞, 经胰蛋白酶消化后传代培养扩增, 倒置相差显微镜观察细胞生长状态, 细胞免疫组化检测CD73、CD34。结果表明, 成功建立了兔BMSCs体外分离及培养扩增的方法, 发现BMSCs表现贴壁生长, P0代时呈集落生长, 细胞呈梭形, 传代后细胞增殖速度加快, 形态开始多样化;细胞免疫组化显示BMSCs表达CD73, 未表达CD34, 反复传代后CD73表达效率增高。上述研究表明, Percoll密度梯度离心联合骨髓贴壁法, 能有效分离、纯化和扩增BMSCs, 提取的细胞具有BMSCs的生长特性和抗原表型, 其中P3~P6代细胞增殖能力强, 可用于进一步的研究工作。This study established the method for isolation, amplification and identification of the rabbit bone marrow mesenchymal stem cells (BMSCs) in vitro, which provided an experimental foundation for the further differentiation and the clinical application of BMSCs. Bone marrow was first aspirated from rabbit iliac and the BMSCs were isolated and cultivated by Percoll density gradient centrifugation which formed monolayer adherent cells after inoculation. Subculture amplification was conducted after trypsin digestion, and the growth characteristics of primary and passage cells were observed by inverted phase contrast microscopes, and CD73 and CD34 were detected by immunohistochemistry. The results showed that the rabbit BMSCs were successfully isolated and cultured in vitro, which grew with adherence, and the primary and passage cells were spindle-shaped and grew in colonies. After subculture, the proliferation rate was accelerated and forms began to diversify. Cell immunohistochemistry indicated that CD73 was expressed in BMSCs while CD34 was not, and the expression efficiency was increased after many generations. The above research showed that Percoll density gradient centrifugation combined with bone marrow adherent method was a simple and reliable method to isolate, purify and amplify rabbit BMSCs. The extracted cells had the growth characteristics and antigenic phenotype of BMSCs, of which the cells in P3~P6 generation had strong cell reproductive capacity and could be used for further research work.

論文, Articles

由于秉持古典哲学观念和传统写作技艺，施特劳斯笔下的自然正当到底意味着什么，十分令人费解。像施特劳斯解读古典著作那样的一种对《自然权利与历史》的详细疏解，实属必要。本文通过对《自然权利与历史》前四章的深入剖析，澄清了古典自然正当学说的基本内容和主要特征。以苏格拉底－柏拉图的探究式怀疑主义为底色的古典自然正当观念，既不是一种自然法学说，也不是一般所谓的绝对主义，而是一种苏格拉底式的正义观。通过对“自然保持哲学上的忠诚，这种正义观得以突破犬儒主义与理想主义之间的两难困境，而始终坚守中道。, 论文分为两个部分。第一部分首先从自然正当的角度重新解释了苏格拉底问题。接着，通过参照施特劳斯的其它相关著作，澄清了自然正当与自然法之间的本质区别，这个区别在《自然权利与历史》一书中被刻意模糊了。最后，结合当代德国公法学家卡尔施密特的相关论说，分析了施特劳斯对“例外状态的简短讨论。施特劳斯与施密特曾经有过短暂的交锋，该部分对例外状态的阐释，也是对两者思想差异的另一种解读。, 论文第二部分主要在于说明，古典的自然正当学说如何面对三种强有力的挑战，即习俗主义、神圣启示和历史主义。习俗主义的代表是伊壁鸠鲁主义，其内核是享乐主义。习俗主义之所以否认自然正当，根源在于它主张一种唯物主义的世界观，将感官快乐奉为最高的善。通过从理论上复原前科学的政治世界，施特劳斯证明了哲学产生的必然性与正当性，以及哲学对于启示的合理拒绝。与以上两种否定自然正当的立场相比，历史主义是偶然产生的，是现代精神的特殊产物，但却是当前挡在自然正当观念面前的最大障碍。通过融合施特劳斯其它著作中的相关信息，本文详细梳理了历史主义从出现到成熟的全过程。鉴于历史主义的最终结果是海德格尔的生存哲学，所以这一梳理同时也简单回答了早期现代哲人的“不懈努力如何最终成就了海德格尔的生存主义。, This dissertation examines Leo Strauss's teaching of natural right through a close reading of the first half of one of his masterpieces Natural Right and History. Due to the dialectic and "historical" character of its argument, "what natural right actually was for Strauss is surprisingly difcult to say". Contrary to the general impression, this dissertation finds that Leo Strauss's natural right doctrine is neither a type of natural law teaching nor any kind of absolutism, but a Socratic idea of justice which by insisting on a philosophic loyalty to "nature" avoids "cynicism" on the one hand and "idealism" on the other., This dissertation consists of two parts. The first part, dealing with the specific properties of Strauss's natural right teaching, is divided into three sections. Firstly, the "Socratic problem" of Strauss is reinterpretated from the point of view of natural right. Secondly, the essential differences between natural right and natural law, which are on the surface blured in NRH, are elaborated by detailed textual analysis of both NRH and Strauss's other relevant essays. In the third section, Strauss's discussion of "extreme situation" in NRH is examined in contrast with the views of Carl Schmitt on the same subject which are now becoming increasingly popular both in China and the western world., The second part of the dissertation is devoted to three objections of natural right, i.e., conventionalism, divine revelation and historicism, while the first two of which may be regarded as eternal alternatives to natural right, the last one is characteristic of modern philosophy. Corresponding to each of the three sections, the hedonism of Epicurus, the conflict between reason and faith and the radical historicism of Heidegger are treated with special attention., Detailed summary in vernacular field only., 李明坤., Thesis (Ph.D.)--Chinese University of Hong Kong, 2012., Includes bibliographical references (leaves 121-131)., s in Chinese and English., Li Mingkun., 前言 --- p.1, Chapter 第一部分 --- 何为自然正当？ --- p.22, Chapter 第一章 --- 苏格拉底问题 --- p.25, Chapter 第二章 --- 自然正当与自然法 --- p.33, Chapter 2.1 --- 古典自然正当学说的基本内容 --- p.33, Chapter 2.2 --- 自然法问题 --- p.41, Chapter 第三章 --- 论例外状态 --- p.48, Chapter 3.1 --- 自然正当与“例外问题 --- p.48, Chapter 3.2 --- 例外状态与施密特 --- p.53, Chapter 第二部分 --- 反驳与回应 --- p.60, Chapter 第四章 --- 习俗主义 --- p.60, Chapter 4.1 --- 城邦：习俗还是自然？ --- p.64, Chapter 4.2 --- 快乐与美德 --- p.68, Chapter 4.3 --- 伊壁鸠鲁主义与古今之争 --- p.71, Chapter 第五章 --- 启示问题 --- p.75, Chapter 5.1 --- 《自然权利与历史》章二的主题 --- p.77, Chapter 5.2 --- 韦伯立场的根基及其政治哲学含义 --- p.80, Chapter 5.3 --- 启示与哲学的冲突及其解决 --- p.85, Chapter 第六章 --- 历史主义 --- p.93, Chapter 6.1 --- 早期现代哲学的历史转向 --- p.94, Chapter 6.2 --- 历史学派及其现代预设 --- p.100, Chapter 6.3 --- 历史主义的哲学化 --- p.108, 结语 --- p.119, 参考文献 --- p.122, http://library.cuhk.edu.hk/record=b5549346, Use of this resource is governed by the terms and conditions of the Creative Commons “Attribution-NonCommercial-NoDerivatives 4.0 International” License (http://creativecommons.org/licenses/by-nc-nd/4.0/)

論文, Articles

[[abstract]]本論文是以硫化法成長硫化銅銦（CuInS2）薄膜，此方法需先成長一銅銦預沈積層然後再進行硫化。本論文以銅銦原子為0.55的比例來作預沉積，然後我們使用X光繞射儀與掃瞄式電子顯微鏡去分析硫化銅銦結晶狀況。 溫度在400℃至450℃之間硫化60分鐘， 可得到較多CuInS2的相而產生較少的Cu-S或In-S系列的二次相產生；而500℃硫化則會使薄膜分解。另外，銦在硫化過程中會因為長時間與高溫度的硫化，使得銦在硫化過程中快速的流失，而在硫化結束後導致銦不足的現象，在此可藉由預沈積層的調配來補足銦的散失。, [[abstract]]The thesis has a proposal for CuInS2-based solar cells are prepared by a rapid thermal sulfide process. All samples are pre-prepared under Cu excess with atomic ratio Cu/In = 0.55 on sola glass. The CuInS2 thin film preparation with Cu/In layers put into thermal sulfur vapor to sulfide. The CuInS2 thin film form by this process has well crystalline, as seen from XRD and SEM. The conversion rate of CuInS2 thin film solar cell has reached 11.4%. The optimal sulfide condition is at sulfur vapor from 400?aC to 450?aC for 60 minutes. This method can produce more CuInS2 form and less Cu-S or In-S form, but the CuInS2 film is resolved at 500?aC. In elements will loss in sulfide procedure. The surplus indium will be deposited to Cu/In film in pre-prepared procedure for remaining Cu/In ratio. We successfully have prepared high purified CuInS2 thin film by rapid thermal sulfide process.

[[abstract]]本套生理訊號量測分析回饋系統軟體方面，我們採用LabVIEW TM 6.1。，硬體方面，主要以BP-508為測量設備，與成功大醫院的精神科醫師合作， 透過這套系統，能及時將人體身上的生理訊號數據擷取出來，經由轉換而及時建立病患資料庫與圖形化，提供醫師對於病人的病情，預防、治療及後續的追蹤有更正確的了解與判斷。

利用ＲＴ－ＰＣＲ技術克隆了番茄ＡＣＣ氧化酶基因ＬＥＥＴＨＹＢＲ編碼區０．９ｋｂ的ｃＤＮＡ片段，經酶切圖譜分析和序列分析鑒定后，反向插入到植物表達載體ｐＢｉｎ４３８中，構建了表達ＡＣＣ氧化酶反義ＲＮＡ的二元載體。用農桿菌侵染美洲黑楊葉片，在含卡那霉素的ＭＳ培養基上選擇轉化子和植株再生，通過ＰＣＲ檢測篩選到１６株轉基因楊樹植株，Ｓｏｕｔｈｅｒｎｂｌｏｔ分析初步確證了外源基因是以單拷貝插入到楊樹基因組中；對楊樹幼苗乙烯釋放量的測定結果表明轉基因楊樹的乙烯釋放量為對照植株的２８％。

利用ＲＴ ＰＣＲ技術克隆了大豆ＡＣＣ合酶基因ＧＭＣＡＣＣＳＩ編碼區１５ｋｂ的ｃＤＮＡ片段，經酶切圖譜分析和序列分析鑒定后，反向插入到２元載體ｐＢｉｎ４３８中，構建了表達ＡＣＣ合酶反義ＲＮＡ的植物表達載體，轉化農桿菌后用該農桿菌侵染美洲黑楊葉片，在含卡那霉素的ＭＳ培養基上選擇轉化子和植株再生，通過ＰＣＲ檢測從抗卡那植株中選到１５株轉基因植株，Ｓｏｕｔｈｅｒｎ雜交分析初步確證了外源基因是以單拷貝插入到楊樹基因組ＤＮＡ中；對楊樹幼苗乙烯釋放的測定結果表明轉基因楊樹幼苗的乙烯釋放量為對照的２２％。

对深圳福田红树林群落两个优势种桐花树(Aegicerascorniculatum)和秋茄(Kandeli-acandel)进行横剖树干解析，研究这两个树种的胸径、地径、树高、材积生长量的年变化规律及形数年变化规律，结果为：胸径、地径、树高、材积生长量呈镊合状曲线态；其年生长量一般在其最大值的50%~98%之间；各种平均生长量随年龄的增加而平缓地增加，近峰值而未及，可见两树种有相当长的成熟及衰老期，它们的现存年龄为20a左右；两树种的基部形数不随年龄的增加而呈规律性的变化，很难确定其经验形数值. The mangrove community in Futian Nature Reserve, Shenzhen City, Guangdong province, is dominated by Aegiceras cormiculatum and Kandeha candel. The growth of these two plant species was investigated by stem-analysis. The mean annual increment and cumulative increment in tree height, stem basal and breast height diameter of these two species were compared. The cumulative increment of these trees increased with age, toward the peak values. Both species were about 20 years old and had reached their mature slatus for some time

李明恂., Thesis (M.A.)--香港中文大學., Reprint., Includes bibliographical references (leaves 115-123)., Li Mingxun., Thesis (M.A.)--Xianggang Zhong wen da xue., 嗚謝 --- p.I, 英文摘要 --- p.II, 附圖目錄 --- p.III, 附表目錄 --- p.VII, Chapter 第一章 --- 前言 --- p.1, Chapter 1. --- 硏究動機 --- p.2, Chapter 2. --- 硏究問題 --- p.4, Chapter 3. --- 硏究對象 --- p.5, Chapter 4. --- 本硏究的效果 --- p.8, Chapter 第二章 --- 人口年齡結構 --- p.11, Chapter 第三章 --- 香港人口的年齡結構 --- p.19, Chapter 1. --- 香港人口的年齡結構 --- p.19, Chapter 2. --- 香港各大統計區人口年齡結構 --- p.23, Chapter 3. --- 香港各統計區人口年齡結構 --- p.28, Chapter 第四章 --- 研究方法 --- p.40, Chapter 1. --- 引言 --- p.40, Chapter 2. --- 年齡結搆指數 --- p.40, Chapter 3. --- 統計方法 --- p.47, Chapter 4. --- 資料處理 --- p.48, Chapter 5. --- 硏究假設 --- p.58, Chapter 6. --- 總結 --- p.59, Chapter 第五章 --- 年齡結構指數 --- p.60, Chapter 1. --- 年齡結構指數數字 --- p.60, Chapter "2," --- 年齡結構指數的空間分佈 --- p.70, Chapter 第六章 --- 年齡結構指數與人口、社會及家庭變數 --- p.82, Chapter 1. --- 簡單相關 --- p.83, Chapter 2. --- 複迴歸 --- p.87, Chapter 3. --- 逐步迴歸 --- p.88, Chapter 4. --- 殘差分析 --- p.91, Chapter 5. --- 總結 --- p.95, Chapter 第七章 --- 總結 --- p.98, Chapter 1. --- 硏究總結 --- p.98, Chapter 2. --- 建議 --- p.100, Chapter 3. --- 進一步的硏究方向 --- p.103, 註釋 --- p.109, 參考書目 --- p.115, http://library.cuhk.edu.hk/record=b5887495, Use of this resource is governed by the terms and conditions of the Creative Commons “Attribution-NonCommercial-NoDerivatives 4.0 International” License (http://creativecommons.org/licenses/by-nc-nd/4.0/)

河合文庫, 河合文庫//101, 1枚 ; 44.8×52.8cm, 嘉慶4, 文書, 1799年(嘉慶4、正祖23)2月、韓益秀が、李明春より、石串里(現在のソウル市城北区)の堂字号の東犯五等直の水田3斗落只、長さ95尺、幅19尺、7負2束を買い、受け取った明文。価格は銅銭85両。, 附属図書館・人文科学研究所・韓国高麗大学校「韓国古文献の調査及び解題及びデジタルイメージの構築事業に関する協定」により電子化, 京都大学附属図書館 Main Library, Kyoto University || https://rmda.kulib.kyoto-u.ac.jp/reuse