1. The Effect of Streptomycin on Freezing Rainbow Trout (Oncorhynchus mykiss) Sperm
- Author
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Ekici, Aygül, Baran, Alper, Özdaş, Ö. Banu, Sandal, A. İzem, Yamaner, Güneş, Güven, Erdoğan, Baltacı, Muhammed Ali, Ekici, Aygül, Baran, Alper, Özdaş, Ö. Banu, Sandal, A. İzem, Yamaner, Güneş, Güven, Erdoğan, and Baltacı, Muhammed Ali
- Abstract
Mature male and female rainbow trout (Oncorhynchus mykiss) were used to study the effect of different concentrations of streptomycin in a cryopreservation solution on the sperm motility, motility duration, and fertilization rate of rainbow trout. 300mM glucose and 10% DMSO containing diluent was used as a cryopreservation solution. Three different concentrations (3600, 7200 and 10800 μg/ml) of streptomycin were added to the diluent. Our study showed that various concentrations of streptomycin in cryopreservation solution caused a significant reduction in the percentage and duration of rainbow trout spermatozoa motility after freezing-thawing. The percentage of egg fertilization also decreased. When streptomycin-containing groups were compared with each other, a statistical difference was found (p<0.05). However, no statistical difference was observed in terms of the percentage of eyed-embryos.
- Published
- 2014