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8 results on '"Chinese Academy of Medical Sciences"'

1. Species-Level Analysis of Human Gut Microbiota With Metataxonomics

Author

Ministry of Science and Technology of the People's Republic of China, Chinese Academy of Medical Sciences, Yang, Jing, Pu, Ji, Lu, Shan, Bai, Xiangning, Wu, Yangfeng, Jin, Dong, Cheng, Yanpeng, Zhang, Gui, Zhu, Wentao, Luo, Xuelian, Rosselló-Mora, Ramón, Xu, Jianguo, Ministry of Science and Technology of the People's Republic of China, Chinese Academy of Medical Sciences, Yang, Jing, Pu, Ji, Lu, Shan, Bai, Xiangning, Wu, Yangfeng, Jin, Dong, Cheng, Yanpeng, Zhang, Gui, Zhu, Wentao, Luo, Xuelian, Rosselló-Mora, Ramón, and Xu, Jianguo

Abstract

The current understanding of human gut microbial community is mainly limited to taxonomic features at the genus level. Here, we examined the human gut microbial community at the species level by metataxonomics. To achieve this purpose, a high-throughput approach involving operational phylogenetic unit analysis of the near full-length 16S ribosomal RNA (rRNA) gene sequence was used. A total of 1,235 species-level phylotypes (SLPs) were classified in the feces of 120 Chinese healthy individuals, including 461 previously classified species, 358 potentially new species, and 416 potentially new taxa, which were categorized into low, medium, and high prevalent bacteria groups based on their prevalence. Each individual harbored 186 ± 51 SLPs on average. There was no universal bacterial species shared by all the individuals. However, 90 ± 19 of 116 SLPs were shared in the high prevalent bacteria group. Thirty-two out of thirty-eight species in the high prevalent bacteria group detected in this study were also found in at least one previous study on human gut microbiota based on either culture-dependent or culture-independent approaches. Through compositional analysis, a hierarchical clustering of the prevalence and relative abundance of the 1,235 SLPs revealed two types of gut microbial communities, which were dominated by Prevotella copri and Bacteroides vulgatus, respectively. The type dominated by P. copri was more prevalent in northern China, while the B. vulgatus-dominant type was more prevalent in southern China. Therefore, P- and B-type gut microbial communities in China were proposed. It was found that 166 out of 461 known bacterial species have been previously reported as potential pathogens, and the individuals sampled for this study harbored 20 of these potential pathogenic species on average. The top two most abundant and prevalent potential pathogenic species were Klebsiella pneumoniae and Bacteroides fragilis.

Published
2020

2. China Health and Retirement Longitudinal Study（2011 baseline）

Author

Yaohui Zhao (Ational School Of Development Of Peking University), John Strauss (Economic Institute,University Of Southern California ), Gonghuan Yang (School Of China Union Medical University, Chinese Academy Of Medical Sciences), Yaohui Zhao (Ational School Of Development Of Peking University), John Strauss (Economic Institute,University Of Southern California ), and Gonghuan Yang (School Of China Union Medical University, Chinese Academy Of Medical Sciences)

Published
2015

3. The China Health and Retirement Longitudinal Study（2012 pilot wave2)

Author

Yaohui Zhao (National School Of Development Of Peking University), John Strauss (Economic Institute,University Of Southern California ), Gonghuan Yang (School Of China Union Medical University, Chinese Academy Of Medical Sciences), Yaohui Zhao (National School Of Development Of Peking University), John Strauss (Economic Institute,University Of Southern California ), and Gonghuan Yang (School Of China Union Medical University, Chinese Academy Of Medical Sciences)

Published
2015

4. China Health and Retirement Longitudinal Study（2013 wave2）

Author

Yaohui Zhao (National School Of Development Of Peking University), John Strauss (Economic Institute,University Of Southern California ), Gonghuan Yang (School Of China Union Medical University, Chinese Academy Of Medical Sciences), Yaohui Zhao (National School Of Development Of Peking University), John Strauss (Economic Institute,University Of Southern California ), and Gonghuan Yang (School Of China Union Medical University, Chinese Academy Of Medical Sciences)

Published
2015

5. The China Health and Retirement Longitudinal Study（2012 pilot wave2)

Author

Yaohui Zhao (National School Of Development Of Peking University), John Strauss (Economic Institute,University Of Southern California ), Gonghuan Yang (School Of China Union Medical University, Chinese Academy Of Medical Sciences), Yaohui Zhao (National School Of Development Of Peking University), John Strauss (Economic Institute,University Of Southern California ), and Gonghuan Yang (School Of China Union Medical University, Chinese Academy Of Medical Sciences)

Published
2015

6. China Health and Retirement Longitudinal Study（2013 wave2）

Author

Yaohui Zhao (National School Of Development Of Peking University), John Strauss (Economic Institute,University Of Southern California ), Gonghuan Yang (School Of China Union Medical University, Chinese Academy Of Medical Sciences), Yaohui Zhao (National School Of Development Of Peking University), John Strauss (Economic Institute,University Of Southern California ), and Gonghuan Yang (School Of China Union Medical University, Chinese Academy Of Medical Sciences)

Published
2015

7. China Health and Retirement Longitudinal Study（2011 baseline）

Author

Yaohui Zhao (Ational School Of Development Of Peking University), John Strauss (Economic Institute,University Of Southern California ), Gonghuan Yang (School Of China Union Medical University, Chinese Academy Of Medical Sciences), Yaohui Zhao (Ational School Of Development Of Peking University), John Strauss (Economic Institute,University Of Southern California ), and Gonghuan Yang (School Of China Union Medical University, Chinese Academy Of Medical Sciences)

Published
2015

8. Puerarin Suppresses Angiotensin II-Induced Cardiac Hypertrophy by Inhibiting NADPH Oxidase Activation and Oxidative Stress-Triggered AP-1 Signaling Pathways

Author

Gang, Chen; Beijing Chao-Yang Hospital, Capital Medical University, Beijing, China., Qiang, Cao; Clinical College of Traditional Chinese Medicine, Gansu University of Chinese Medicine, Lanzhou, China., Xiangli, Cui; Beijing Chao-Yang Hospital, Capital Medical University, Beijing, China., Shifen, Pan; Beijing Chao-Yang Hospital, Capital Medical University, Beijing, China., Chong, Shen; Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, China., Lihong, Liu; Beijing Chao-Yang Hospital, Capital Medical University, Beijing, China., Gang, Chen; Beijing Chao-Yang Hospital, Capital Medical University, Beijing, China., Qiang, Cao; Clinical College of Traditional Chinese Medicine, Gansu University of Chinese Medicine, Lanzhou, China., Xiangli, Cui; Beijing Chao-Yang Hospital, Capital Medical University, Beijing, China., Shifen, Pan; Beijing Chao-Yang Hospital, Capital Medical University, Beijing, China., Chong, Shen; Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, China., and Lihong, Liu; Beijing Chao-Yang Hospital, Capital Medical University, Beijing, China.

Abstract

PURPOSE. To examine the effects of puerarin (Pue) on angiotensin II (AngII)-induced nicotinamide adenine dinucleotide phosphate (NADPH) oxidase activation and oxidative stress-related signaling pathways in the hypertrophic response of cardiomyocytes. METHODS. Primary cardiomyocytes of neonatal C57BL/6J mice were pretreated with Pue (50, 100 μmol/L) and were then stimulated with AngII 1 μmol/L. NADPH oxidase activity and reactive oxygen species (ROS) levels were measured by lucigenin-enhanced chemiluminescence assay and flow cytometry. Western blotting was used to detect the distribution of the oxidase subunits, extracellular signal-regulated kinase (ERK1/2) and c-jun N-terminal kinase (JNK1/2) activation, and an electrophoretic mobility shift assay (EMSA) was performed to analyze the DNA binding activity of activator protein-1 (AP-1). Adult C57BL/6J mice were infused with AngII and were administered with Pue (100, 200 mg·kg-1·d-1) for 15 d. After the treatment, systolic blood pressure (SBP) and left ventricular wall thickness were examined. The ratios of heart weight to body weight (HW/BW) and left ventricular weight to body weight (LVW/BW) were measured, and heart morphometry was assessed. RESULTS. In vitro, Pue dose-dependently blocked the phosphorylation of ERK1/2 and JNK1/2 and eventually abolished AP-1 binding activity through the inhibition of ROS production. Further studies revealed that AngII treatment resulted in increased NADPH oxidase activity, which was suppressed by Pue via the disruption of Rac1 activation and membrane translocation of oxidase subunits. In vivo, Pue attenuated cardiac hypertrophy, as evaluated by decreased HW/BW, LVW/BW, myocyte surface area, and left ventricular wall thickness. CONCLUSIONS. The anti-hypertrophic mechanism of Pue occurred by blocking Rac1-dependent NADPH oxidase activation and downstream redox-sensitive AP-1 signaling pathways. This article is open to POST-PUBLICATION REVIEW. Registered readers (see “For Readers”) may

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