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Contains fulltext : 222207.pdf (publisher's version ) (Open Access)

Contains fulltext : 222207.pdf (publisher's version ) (Open Access)

Contains fulltext : 222207.pdf (publisher's version ) (Open Access)

Mycobacterium tuberculosis remains one of the most problematic infectious agents, owing to its highly developed mechanisms to evade host immune responses combined with the increasing emergence of antibiotic resistance. Host-directed therapies aiming to optimize immune responses to improve bacterial eradication or to limit excessive inflammation are a new strategy for the treatment of tuberculosis. In this study, we have established a zebrafish-Mycobacterium marinum natural host-pathogen model system to study induced protective immune responses in mycobacterial infection. We show that priming adult zebrafish with heat-killed Listeria monocytogenes (HKLm) at 1 day prior to M. marinum infection leads to significantly decreased mycobacterial loads in the infected zebrafish. Using rag1−/− fish, we show that the protective immunity conferred by HKLm priming can be induced through innate immunity alone. At 24 h post-infection, HKLm priming leads to a significant increase in the expression levels of macrophage-expressed gene 1 (mpeg1), tumor necrosis factor α (tnfa) and nitric oxide synthase 2b (nos2b), whereas superoxide dismutase 2 (sod2) expression is downregulated, implying that HKLm priming increases the number of macrophages and boosts intracellular killing mechanisms. The protective effects of HKLm are abolished when the injected material is pretreated with nucleases or proteinase K. Importantly, HKLm priming significantly increases the frequency of clearance of M. marinum infection by evoking sterilizing immunity (25 vs 3.7%, P=0.0021). In this study, immune priming is successfully used to induce sterilizing immunity against mycobacterial infection. This model provides a promising new platform for elucidating the mechanisms underlying sterilizing immunity and to develop host-directed treatment or prevention strategies against tuberculosis.

© 2018 The Author(s). Background: There is a significant deficiency of national health information for Indigenous peoples in Canada. This manuscript describes the Community Profile Survey (CPS), a community-based, national-level survey designed to identify and describe existing healthcare delivery, funding models, and diabetes specific infrastructure and programs in Indigenous communities. Methods: The CPS was developed collaboratively through FORGE AHEAD and the First Nations and Inuit Health Branch of Health Canada. Regional and federal engagement and partnerships were built with Indigenous organizations to establish regionally-tailored distribution of the 8-page CPS to 440 First Nations communities. Results were collected (one survey per community) and reported in strata by region, with descriptive analyses performed on all variables. Results were shared with participating communities and regional/federal partners through tailored reports. Results: A total of 84 communities completed the survey (19% response rate). The majority of communities had a health centre/office to provide service to their patients with diabetes, with limited on-reserve hospitals for ambulatory or case-sensitive conditions. Few healthcare specialists were located on-site, with patients frequently travelling off-site (> 40 km) for diabetes-related complications. The majority of healthcare professionals on-site were Health Directors, Community Health Nurses, and Home Care Nurses. Many communities had a diabetes registry but few reported a diabetes surveillance system. Regional variation in healthcare services, diabetes programs, and funding models were noted, with most communities engaging in some type of innovative strategy to improve care for patients with diabetes. Conclusions: The CPS is the first community-based, national-level survey of its kind in Canada. Although the response rate was low, the CPS was distributed and successfully administered across a broad range of First Nations com

© 2018 The Author(s). Background: There is a significant deficiency of national health information for Indigenous peoples in Canada. This manuscript describes the Community Profile Survey (CPS), a community-based, national-level survey designed to identify and describe existing healthcare delivery, funding models, and diabetes specific infrastructure and programs in Indigenous communities. Methods: The CPS was developed collaboratively through FORGE AHEAD and the First Nations and Inuit Health Branch of Health Canada. Regional and federal engagement and partnerships were built with Indigenous organizations to establish regionally-tailored distribution of the 8-page CPS to 440 First Nations communities. Results were collected (one survey per community) and reported in strata by region, with descriptive analyses performed on all variables. Results were shared with participating communities and regional/federal partners through tailored reports. Results: A total of 84 communities completed the survey (19% response rate). The majority of communities had a health centre/office to provide service to their patients with diabetes, with limited on-reserve hospitals for ambulatory or case-sensitive conditions. Few healthcare specialists were located on-site, with patients frequently travelling off-site (> 40 km) for diabetes-related complications. The majority of healthcare professionals on-site were Health Directors, Community Health Nurses, and Home Care Nurses. Many communities had a diabetes registry but few reported a diabetes surveillance system. Regional variation in healthcare services, diabetes programs, and funding models were noted, with most communities engaging in some type of innovative strategy to improve care for patients with diabetes. Conclusions: The CPS is the first community-based, national-level survey of its kind in Canada. Although the response rate was low, the CPS was distributed and successfully administered across a broad range of First Nations com

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Most of the studies investigating the diagenetic trajectory of fossil bones focus on open-air sites and very little work have been published in confined environments such as catacombs. While the stable thermal history of catacombs should favor bone preservation, the accumulation of corpses over a short period of time could favor their destruction. The aim of this study is to describe the diagenetic trajectory of 128 human bone samples coming from six different burial chambers of the catacomb of Sts Peter and Marcellinus (SSPM, Rome, Italy). A multi-proxy approach was undertaken to provide an assessment of the molecular preservation as well as a direct record of the isotopic composition itself. Collagen yield, carbon and nitrogen abundances, C:N ratio, FT-IR based collagen and carbonate contents and crystallinity index, radiocarbon dating and stable isotope analysis of bone collagen and carbonate indicate that both the mineral and the organic fractions are impacted by diagenesis to various degrees, and that bones originating from the small burial chambers are more affected than those coming from the large ones. While some of the bones were strongly recrystallized, the impact of bone diagenesis on the stable isotope values of bone carbonate was limited. Comparison with contemporary sites from the Latium showed that conditions prevailing in catacombs seem overall to favor, rather than disadvantage bone preservation., info:eu-repo/semantics/published

Within SARNET, the corium topic covers all the behaviors of corium from early phase of core degradation to in or ex-vessel corium recovery with the exception of corium interaction with water, direct containment heating and fission product release. The corium topic regroups in three work packages the critical mass of competence required to improve significantly the corium behavior knowledge. The spirit of the SARNET networking is to share the knowledge, the facilities and the simulation tools for severe accidents, so to reach a better efficiency and to rationalize the R&D effort at European level. Extensive benchmarking has been launched in most of the areas of research. These benchmarks were mainly dedicated to the recalculation of experiments, while, in the next periods, a larger focus will be given to integral experiments or reactor applications. Eventually, all the knowledge will be accumulated in the ASTEC severe accident simulation code through physical model improvements and extension of validation database. This paper summarizes the progress that has been achieved in the frame of the networking activities. A special focus is placed on the melt pool and debris coolability and corium-concrete interaction, in which, the effects due to multidimensional geometries and heterogeneities has been shown, during SARNET, to play a crucial role and for which further research is still needed., QC 20141111

Electrical Engineering, Mathematics and Computer Science

Electrical Engineering, Mathematics and Computer Science

We present new grids of pre-main sequence (PMS) tracks for stars in the mass range 0.1 to 7.0 Msun. The computations were performed for four different metallicities (Z=0.01, 0.02, 0.03 and 0.04). A fifth table has been computed for the solar composition (Z=0.02), including a moderate overshooting. We describe the update in the physics of the Grenoble stellar evolution code which concerns mostly changes in the equation of state (EOS) adopting the formalism proposed by Pols et al. (1995) and in the treatment of the boundary condition. Comparisons of our models with other grids demonstrate the validity of this EOS in the domain of very low-mass stars. Finally, we present a new server dedicated to PMS stellar evolution which allows the determination of stellar parameters from observational data, the calculation of isochrones, the retrieval of evolutionary files and the possibility to generate graphic outputs. WWW site : http://www-laog.obs.ujf-grenoble.fr/activites/starevol/evol.html, Comment: 7 pages, 3 figures, accepted for publication in A&A Main Journal

We present new grids of pre-main sequence (PMS) tracks for stars in the mass range 0.1 to 7.0 Msun. The computations were performed for four different metallicities (Z=0.01, 0.02, 0.03 and 0.04). A fifth table has been computed for the solar composition (Z=0.02), including a moderate overshooting. We describe the update in the physics of the Grenoble stellar evolution code which concerns mostly changes in the equation of state (EOS) adopting the formalism proposed by Pols et al. (1995) and in the treatment of the boundary condition. Comparisons of our models with other grids demonstrate the validity of this EOS in the domain of very low-mass stars. Finally, we present a new server dedicated to PMS stellar evolution which allows the determination of stellar parameters from observational data, the calculation of isochrones, the retrieval of evolutionary files and the possibility to generate graphic outputs. WWW site : http://www-laog.obs.ujf-grenoble.fr/activites/starevol/evol.html, Comment: 7 pages, 3 figures, accepted for publication in A&A Main Journal

Appartient à l’ensemble documentaire : IconMUS1, Appartient à l’ensemble documentaire : IconMUSNum, Appartient à l’ensemble documentaire : IconMUS0