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In investigation of (patho)physiological processes, cells represent frequently used analyte as an exceptional source of information. However, spectroscopic analysis of live cells is still very seldom in clinics, as well as in research studies. Among others, the reasons are long acquisition time during which autolysis process is activated, necessity of specified technical equipment, and inability to perform analysis in a moment of sample preparation. Hence, an optimal method of preserving cells in the existing state is of extreme importance, having in mind that selection of fixative is cell lineage dependent. In this study, two commonly used chemical fixatives, formaldehyde and methanol, are used for preserving primary mesenchymal stem cells extracted from periodontal ligament, which are valuable cell source for reconstructive dentistry. By means of Raman spectroscopy, cell samples were probed and the impact of these fixatives on their Raman response was analyzed and compared. Different chemical mechanisms are the core processes of formaldehyde and methanol fixation and certain Raman bands are shifted and/or of changed intensity when Raman spectra of cells fixed in that manner are compared. In order to get clearer picture, comprehensive statistical analysis was performed.

We have employed micro-Raman spectroscopy to get insight into intrinsic biomolecular profile of individual mesenchymal stem cell isolated from periodontal ligament. Furthermore, these cells were stimulated towards adipogenic, chondrogenic, and osteogenic lineages and their status of differentiation was assessed using micro-Raman spectroscopy. In both cases, glass coverslips were used as substrates, due to their wide availability and cost effectiveness. In all sample groups, the same type of behavior was observed, manifested as changes in Raman spectra: the increase of relative intensity of protein/lipid bands and decrease of nucleic acid bands. Comprehensive statistical analysis in the form of principal component analysis was performed, which revealed noticeable grouping of cells with the similar features. Despite the inhomogeneity of primary stem cells and their differentiated lineages, we demonstrated that micro-Raman spectroscopy is sufficient for distinguishing cells' status, which can be valuable for medical and clinical application.

We have employed micro-Raman spectroscopy to get insight into intrinsic biomolecular profile of individual mesenchymal stem cell isolated from periodontal ligament. Furthermore, these cells were stimulated towards adipogenic, chondrogenic, and osteogenic lineages and their status of differentiation was assessed using micro-Raman spectroscopy. In both cases, glass coverslips were used as substrates, due to their wide availability and cost effectiveness. In all sample groups, the same type of behavior was observed, manifested as changes in Raman spectra: the increase of relative intensity of protein/lipid bands and decrease of nucleic acid bands. Comprehensive statistical analysis in the form of principal component analysis was performed, which revealed noticeable grouping of cells with the similar features. Despite the inhomogeneity of primary stem cells and their differentiated lineages, we demonstrated that micro-Raman spectroscopy is sufficient for distinguishing cells' status, which can be valuable for medical and clinical application.

In investigation of (patho)physiological processes, cells represent frequently used analyte as an exceptional source of information. However, spectroscopic analysis of live cells is still very seldom in clinics, as well as in research studies. Among others, the reasons are long acquisition time during which autolysis process is activated, necessity of specified technical equipment, and inability to perform analysis in a moment of sample preparation. Hence, an optimal method of preserving cells in the existing state is of extreme importance, having in mind that selection of fixative is cell lineage dependent. In this study, two commonly used chemical fixatives, formaldehyde and methanol, are used for preserving primary mesenchymal stem cells extracted from periodontal ligament, which are valuable cell source for reconstructive dentistry. By means of Raman spectroscopy, cell samples were probed and the impact of these fixatives on their Raman response was analyzed and compared. Different chemical mechanisms are the core processes of formaldehyde and methanol fixation and certain Raman bands are shifted and/or of changed intensity when Raman spectra of cells fixed in that manner are compared. In order to get clearer picture, comprehensive statistical analysis was performed.

In investigation of (patho)physiological processes, cells represent frequently used analyte as an exceptional source of information. However, spectroscopic analysis of live cells is still very seldom in clinics, as well as in research studies. Among others, the reasons are long acquisition time during which autolysis process is activated, necessity of specified technical equipment, and inability to perform analysis in a moment of sample preparation. Hence, an optimal method of preserving cells in the existing state is of extreme importance, having in mind that selection of fixative is cell lineage dependent. In this study, two commonly used chemical fixatives, formaldehyde and methanol, are used for preserving primary mesenchymal stem cells extracted from periodontal ligament, which are valuable cell source for reconstructive dentistry. By means of Raman spectroscopy, cell samples were probed and the impact of these fixatives on their Raman response was analyzed and compared. Different chemical mechanisms are the core processes of formaldehyde and methanol fixation and certain Raman bands are shifted and/or of changed intensity when Raman spectra of cells fixed in that manner are compared. In order to get clearer picture, comprehensive statistical analysis was performed.

We have employed micro-Raman spectroscopy to get insight into intrinsic biomolecular profile of individual mesenchymal stem cell isolated from periodontal ligament. Furthermore, these cells were stimulated towards adipogenic, chondrogenic, and osteogenic lineages and their status of differentiation was assessed using micro-Raman spectroscopy. In both cases, glass coverslips were used as substrates, due to their wide availability and cost effectiveness. In all sample groups, the same type of behavior was observed, manifested as changes in Raman spectra: the increase of relative intensity of protein/lipid bands and decrease of nucleic acid bands. Comprehensive statistical analysis in the form of principal component analysis was performed, which revealed noticeable grouping of cells with the similar features. Despite the inhomogeneity of primary stem cells and their differentiated lineages, we demonstrated that micro-Raman spectroscopy is sufficient for distinguishing cells' status, which can be valuable for medical and clinical application.

In investigation of (patho)physiological processes, cells represent frequently used analyte as an exceptional source of information. However, spectroscopic analysis of live cells is still very seldom in clinics, as well as in research studies. Among others, the reasons are long acquisition time during which autolysis process is activated, necessity of specified technical equipment, and inability to perform analysis in a moment of sample preparation. Hence, an optimal method of preserving cells in the existing state is of extreme importance, having in mind that selection of fixative is cell lineage dependent. In this study, two commonly used chemical fixatives, formaldehyde and methanol, are used for preserving primary mesenchymal stem cells extracted from periodontal ligament, which are valuable cell source for reconstructive dentistry. By means of Raman spectroscopy, cell samples were probed and the impact of these fixatives on their Raman response was analyzed and compared. Different chemical mechanisms are the core processes of formaldehyde and methanol fixation and certain Raman bands are shifted and/or of changed intensity when Raman spectra of cells fixed in that manner are compared. In order to get clearer picture, comprehensive statistical analysis was performed.

We have employed micro-Raman spectroscopy to get insight into intrinsic biomolecular profile of individual mesenchymal stem cell isolated from periodontal ligament. Furthermore, these cells were stimulated towards adipogenic, chondrogenic, and osteogenic lineages and their status of differentiation was assessed using micro-Raman spectroscopy. In both cases, glass coverslips were used as substrates, due to their wide availability and cost effectiveness. In all sample groups, the same type of behavior was observed, manifested as changes in Raman spectra: the increase of relative intensity of protein/lipid bands and decrease of nucleic acid bands. Comprehensive statistical analysis in the form of principal component analysis was performed, which revealed noticeable grouping of cells with the similar features. Despite the inhomogeneity of primary stem cells and their differentiated lineages, we demonstrated that micro-Raman spectroscopy is sufficient for distinguishing cells' status, which can be valuable for medical and clinical application.

We report the low-temperature Raman scattering study of racemic ibuprofen. Detailed analysis of the racemic ibuprofen crystal symmetry, related to the vibrational properties of the system, has been presented. The first principle calculations of a single ibuprofen molecule dynamical properties are compered with experimental data. Nineteen, out of 26 modes expected for the spectral region below 200 cm(-1), have been observed.

We report the low-temperature Raman scattering study of racemic ibuprofen. Detailed analysis of the racemic ibuprofen crystal symmetry, related to the vibrational properties of the system, has been presented. The first principle calculations of a single ibuprofen molecule dynamical properties are compered with experimental data. Nineteen, out of 26 modes expected for the spectral region below 200 cm(-1), have been observed.

Production of high quality milk, milk containing low number of bacteria (several thousand to lt 141 000 cells/ml of milk) and low number of somatic cells count (SČ), is the target of Program of control and prevention of mastitis in dairy cows. Program of mastitis control must provide diagnostic tests for evaluation of udder infections by enviromental microorganizams and prevention of new intramammarian infections (IMI). The evidence is presented that longterm, intensive program of teat désinfection after milking and therapy of dryed cows are procedures which are enabling significant decrease of SČ and number of bacteria in 1 ml of milk. Clinical changes of udder are offtenly caused by Corynebacterium bovis, Streptococcus sp. and Staphylococcus sp. which are also responsible for increasment of SČ and IMI cases. Knowing that most of mastitisies are subclinical by character, than it is understandable that various IMI of dairy cows are appearing after usage of the various antiseptics. We have been using accessory remedy for dry milking VETER-D, for prevention of sub-clinical mastitisies, "dry" milking and evaluation of IMI spreadness in udder health ma-nagment program, on 403 dairy cows., Cilj programa i kontrole i suzbijanja mastitisa kod muznih krava je proizvodnja mleka visokog kvaliteta, tj. mleka koje će sadržati mali broj bakterija (od nekoliko hiljada do = 141 000 ćelija/ml mleka) i somatskih ćelija. Program kontrole mastitisa trebalo bi da ima kontrolu infekcija vimena sa organizmima sredine i efikasan sistem praćenja i sprečavanja novih intramamarnih infekcija vimena (MI). Postoje dokazi da dugoročni intenzivni programi dezinfekcije sisa posle muže i terapija zasušenih krava mogu izrazito da smanje broj SČ i bakterija u 1 ml mleka. Kliničke promene na vimenu najčešće izazivaju Corynebacterium bovis, streptokoke i stafiloke i to su faktori koji najviše povećavaju SČ i intramamarne infekcije. Budući daje većina mastitisa subklinička, postoji različit broj intramamarnih infekcija (IMI) vimena muznih krava posle dezinfekcije sisa sa različitim antisepticima. Mi smo upotrbljavaîi pomoćno lekovito sredstvo VETER-D za suzbijanje subkliniČkih mastitisa, "suvu" mužu i ispitivanje rasprostranjenosti IMI u menadžerskom programu zdravlja vimena, na 403 krave.

Preparat prirodnog porekla na bazi nevene, kantariona, hajdučke trave četinarske smole, voska i propolisa, pod nazivom VETER D, koji je prema osnovnim indikacijama namenjen za topikalno lečenje oboljenja kože, korišćen je kao sredstvo za potapanje sisa posle muže u tri stada mlečnih krava sa visokim procentom subkliničkih mastitisa i lošim kvalitetom mleka. Nakon dvomesečne primene zabeleženi su veoma dobri rezultati koji su se ogledali u veoma značajnom smanjenju broja bakterija i somatskih ćelija u mleku, zatim u smanjenom procentu kliničkih i subkliničkih mastitisa.

Production of high quality milk, milk containing low number of bacteria (several thousand to lt 141 000 cells/ml of milk) and low number of somatic cells count (SČ), is the target of Program of control and prevention of mastitis in dairy cows. Program of mastitis control must provide diagnostic tests for evaluation of udder infections by enviromental microorganizams and prevention of new intramammarian infections (IMI). The evidence is presented that longterm, intensive program of teat désinfection after milking and therapy of dryed cows are procedures which are enabling significant decrease of SČ and number of bacteria in 1 ml of milk. Clinical changes of udder are offtenly caused by Corynebacterium bovis, Streptococcus sp. and Staphylococcus sp. which are also responsible for increasment of SČ and IMI cases. Knowing that most of mastitisies are subclinical by character, than it is understandable that various IMI of dairy cows are appearing after usage of the various antiseptics. We have been using accessory remedy for dry milking VETER-D, for prevention of sub-clinical mastitisies, "dry" milking and evaluation of IMI spreadness in udder health ma-nagment program, on 403 dairy cows., Cilj programa i kontrole i suzbijanja mastitisa kod muznih krava je proizvodnja mleka visokog kvaliteta, tj. mleka koje će sadržati mali broj bakterija (od nekoliko hiljada do = 141 000 ćelija/ml mleka) i somatskih ćelija. Program kontrole mastitisa trebalo bi da ima kontrolu infekcija vimena sa organizmima sredine i efikasan sistem praćenja i sprečavanja novih intramamarnih infekcija vimena (MI). Postoje dokazi da dugoročni intenzivni programi dezinfekcije sisa posle muže i terapija zasušenih krava mogu izrazito da smanje broj SČ i bakterija u 1 ml mleka. Kliničke promene na vimenu najčešće izazivaju Corynebacterium bovis, streptokoke i stafiloke i to su faktori koji najviše povećavaju SČ i intramamarne infekcije. Budući daje većina mastitisa subklinička, postoji različit broj intramamarnih infekcija (IMI) vimena muznih krava posle dezinfekcije sisa sa različitim antisepticima. Mi smo upotrbljavaîi pomoćno lekovito sredstvo VETER-D za suzbijanje subkliniČkih mastitisa, "suvu" mužu i ispitivanje rasprostranjenosti IMI u menadžerskom programu zdravlja vimena, na 403 krave.

Preparat prirodnog porekla na bazi nevene, kantariona, hajdučke trave četinarske smole, voska i propolisa, pod nazivom VETER D, koji je prema osnovnim indikacijama namenjen za topikalno lečenje oboljenja kože, korišćen je kao sredstvo za potapanje sisa posle muže u tri stada mlečnih krava sa visokim procentom subkliničkih mastitisa i lošim kvalitetom mleka. Nakon dvomesečne primene zabeleženi su veoma dobri rezultati koji su se ogledali u veoma značajnom smanjenju broja bakterija i somatskih ćelija u mleku, zatim u smanjenom procentu kliničkih i subkliničkih mastitisa.